Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein‐2 on periodontal regeneration

Song, D‐,S.,Park, J‐,C.,Jung, I‐,H.,Choi, S‐,H.,Cho, K‐,S.,Kim, C‐,K.,Kim, C‐,S. Blackwell Publishing Ltd 2011 Journal of periodontal research Vol.46 No.2

<P> <I>Song D‐S, Park J‐C, Jung I‐H, Choi S‐H, Cho K‐S, Kim C‐K, Kim C‐S. Enhanced adipogenic differentiation and reduced collagen synthesis induced by human periodontal ligament stem cells might underlie the negative effect of recombinant human bone morphogenetic protein‐2 on periodontal regeneration. J Periodont Res 2011; 46: 193–203. © 2010 John Wiley & Sons A/S</I> </P><P><B>Background and Objective: </B> Recombinant human bone morphogenetic protein‐2 (rhBMP‐2) is a potent inducer for the regeneration of mineralized tissue, but has a limited effect on the regeneration of cementum and periodontal ligament (PDL). The aim of the present study was to determine the effects of rhBMP‐2 on the <I>in vitro</I> and <I>in vivo</I> biologic activity of well‐characterized human PDL stem cells (hPDLSCs) and to elucidate the underlying mechanism of minimal periodontal regeneration by rhBMP‐2.</P><P><B>Material and Methods: </B> hPDLSCs were isolated and cultured, and then transplanted into an ectopic subcutaneous mouse model using a carrier treated either with or without rhBMP‐2. Comprehensive histologic, histometric and immunohistochemical analyses were performed after an 8‐wk healing period. The effects of rhBMP‐2 on the adipogenic and osteogenic/cementogenic differentiation of hPDLSCs were also evaluated. The effect of rhBMP‐2 on both soluble and insoluble collagen synthesis was analyzed, and the expression of mRNA and protein for collagen types I, II, III and V was assessed.</P><P><B>Results: </B> In the present study, rhBMP‐2 promoted both adipogenic and osteogenic/cementogenic differentiation of hPDLSCs <I>in vitro</I>, and the <I>in vivo</I> potential of hPDLSCs to form mineralized cementum and organized PDL tissue was down‐regulated following treatment with rhBMP‐2. Collagen synthesis, which plays a crucial role in the regeneration of cementum and the periodontal attachment, was significantly reduced, with associated modification of the relevant mRNA and protein expression profiles.</P><P><B>Conclusion: </B> In summary, the findings of the present study suggest that enhanced adipogenic differentiation and inhibition of collagen synthesis by hPDLSCs appear to be partly responsible for the minimal effect of rhBMP‐2 on cementum and PDL tissue regeneration by hPDLSCs.</P>

Particle Balance Study for the Fueling Efficiency in KSTAR Experiments 2013

J-. W. Juhn,S. H. Hahn,S. H. Hong,J. I. Song,Y. O. Kim,K. P. Kim,J. S. Kim 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.65 No.8

The efficiencies of the fueling system in the KSTAR have been studied for the 2013 experimentalresults. The absolute amount of injected fuel or deuterium has been calibrated in advance of themain plasma experiments. This includes the primary actuator of fueling, i.e., gas puffing (GP)with piezoelectric valves located on mid-plane, and supersonic molecular beam injector (SMBI). In addition, neutral beam injectors (NBIs) and their base facility chamber also contribute to theamount of fuel injection whenever they are operated. Low fueling efficiency combined with particleloss due to wall retention essentially results in poor performance of density feedback control magneticfusion devices such as the KSTAR.

중년 사무직 남성의 직무스트레스와 허리둘레, 체질량지수의 관계에 관한 연구

강수임,고보경,김지영,노정연,리청,송주은,이현영,이혜민,최유경,정덕유,이민경 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

Purpose: The purpose of this study was to determine the relationship between job stress, body mass index and waist circumference among middle aged male workers. Methods: Using a descriptive correlation study design, a total of 122 middle aged male workers were recruited through convenience sampling from November 8 to December 13, 2015. The questionnaire included in this study were general characteristics and the Korean Occupational Stress Scale-Short Form (KOSS-SF). Data were statistically analyzed using SPSS windows version 22 program with Pearson’s correlation coefficient. Results: A significant relationship was found between job stress, body mass index (p=.043) and waist circumference (p=.007). Conclusion: These results suggest that high job stress are significantly associated with both body mass index and waist circumference of middle aged male workers. This implies a need for intervention that focuses on reducing obesity and metabolic syndrome influenced by job stress.

한국 임해 공단 연안에서 퇴적물, 해수 및 굴(Crassostrea gigas)의 중금속 함량

이인숙,노분조,송준임,김은정 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-

본 연구는 한국 연안의 오염도를 파악하기 위해 마산만, 온산만, 대산공업 단지 및 각각의 대조구에서 해수, 퇴적물 및 굴의 구리, 납, 아연 및 카드뮴의 농도를 조사하였다. 임해 공단 연안과 각각의 대조구에서의 중금속 농도는 대산공단 지역의 해수를 제외하고는 임해 공단 연안의 중금속 농도가 대조구에서보다 높게 나타났다. 해수내의 용존성 구리, 납, 아연, 및 카드뮴의 농도는 각각 0.3∼1.75㎍/1, 0.013∼0.12㎍/1, 0.20∼6.14㎍/1, 0.007∼0.021㎍/1 범위를 나타냈다. 퇴적물내의 구리, 납, 아연 및 카드뮴의 농도는 12.0∼47.8㎍/g, 6.16∼59.5㎍/g, 43.0∼230㎍/g, 0.52∼11.2㎍/g 범위를 보였다. 굴의 구리, 납, 아연 및 카드뮴 농도는 12.1∼85.6㎍/g, 0.267∼1.49㎍/g, 1,070∼3,250㎍/g, 3.23∼7.71㎍/g 범위로 나타났다. 본 연구에서 임해공단 연안에 있는 굴의 중금속 농도는 Mussel Watch(1992)조사와 비교해 볼 때 크게 오염되지 않은 것으로 사료된다. To investigate the degree of contamination from Korean coastal region the concentration of Cu, Pb, Zn and Cd in sediments, seawater and oyster(Crassostrea gigas)at Masan Bay, Onsan Bay, Daesan industrial complex and their control areas were analysed. Values for sediments, seawater and oyster in the industrial complex coastal region were higher than those in the control area except for seawater in Daesan. The values for dissolved phase of Cu, Pb, Zn and Cd in seawater showed 0.3∼1.75㎍/1, 0.013∼0.12㎍/1, 0.20∼6.14㎍/1 and 0.007∼0.021㎍/1, respectively. The concentrations of Cu, Pb, Zn and Cd in sediments were 12.0∼47.8㎍/g, 6.16∼59.5㎍/g, 43.0∼230㎍/g and 0.52∼11.2㎍/g, respectively. The concentrations of Cu, Pb, Zn and Cd in oyster showed 12.1∼85.6㎍/g, 0.267∼1.48㎍/g, 1,070∼3,250㎍/g, and 3.23∼7.71㎍/g, respectively. The contents of heavy metals in oysters at industrial complex coastal region showed that they were not seriously contaminated compared with those of Mussel Watch(1992).

Detection of Herpes Simplex Virus Type-1 DNA by In Situ Polymerase Chain Reaction

Choi, J. H Korean Dermatological Association 1995 Annals of Dermatology Vol.7 No.3

1987년 한국에서 발생한 렙토스피라병의 혈청역학적 조사

이증훈,박영수,이우곤,김석용,정선식,우준희,박성광,박경희,송영욱,김선영,기정일,최두혁,강성귀,김주완,최강원,김우열,최명식,최인학,장우현,윤성열 대한감염학회 1988 감염 Vol.20 No.3

Human leptospirosis was an unfamiliar disease in Korea until 1984 that outbreak of leptospirosis occurred among farmers and soldiers after field works for harvesting rice. During that time, Lee and Jo confirmed the first Korean cases of leptospirosis by serological test, isolation of causative agent and autopy findings. Afterward several outbreaks occurred also during autumn especially after flood in every years and some characterisitcs of leptospirosis in Korea such as clinical manifestations, serotypes and seroepidemiological features has been revealed by many investigators. Because of the major mode of transmission between rodents and human is by direct contact with leptospiral urine of rodents or contaminated soil by the urine, leptospirosis in Korea has been primarily a disease of person in occupations heavily exposed to contaminated soil or infected urine such as farmer, army and etc. Therefore it seems that leptospirosis is one of the main communicable diseases to be controlled urgently in Korea, for an agricultural people account for almost half of total Korean people. For clarifying the seroepidemiological patterns of human leptospirosis in Korea by sex, month region and main reacting serovars of L. interrogans among acute febrile disease occurred in 1987, 1,773 patient's sers with acute febrile episodes were tested by microagglutination test using 19 representative strains of leptospiral serogroup as antigen. All of those sera were collected from 10 collaborative clinics located in Kyunggi, Kangwon, Chungbuk, Chungnam, Chonbuk, Chonnam province and Seoul. The results wee summerized as follows. 1) Among 1,773 sera of patients with acute febrile episodes, 219 (12.4%) were seropositive to L. interrogans, 487(27.5%) to R. tsutsugamushi, 241(13.6%) to R.typhi and 160(90.0%) to Hantaan virus. 2) Among seropositives to L.interrogans, the male outnumbered the female, 65% and 35%. 3) For age distribution, 26.9% of seropositives to L.interrogans were fifties, 19.6% were forties, 9.1% were sixties, 5.9% were thirties and 4.1% were twenties. 4) Eighty three percent of seropositives had occurred between September and October in 1987 with a peak in September. 5) Main leptospiral serovars reactive to patient's sera were Icterohaemorrhagiae(54.3%), Canicola(31.0%), CH-48(13.2%), Tarassovi(0.9%)and Cynopteri(0.5%). 6) For regional distribution, 65.8% of seropositives to L.interrogans were residents from Chonbuk, 12.3% were Chonnam, 7.3% were Chungnam, 5.5% were Kyunggi and 1.4% were Kangwon.

한국 임해 공단 연안에서 퇴적물, 해수 및 굴(Crassostrea gigas)의 중금속 함량

김은정,송준임,노분조,이인숙 이화여자대학교 환경문제연구소 1997 이화환경연구 Vol.1 No.-

본 연구는 한국 연안의 오염도를 파악하기 위해 마산만, 온산만, 대산공업 단지 및 각각의 대조구에서 해수, 퇴적물 및 굴의 구리, 납, 아연 및 카드뮴의 농도를 조사하였다. 임해 공단 연안과 각각의 대조구에서의 중금속 농도는 대산공단 지역의 해수를 제외하고는 임해 공단 연안의 중금속 농도가 대조구에서보다 높게 나타났다. 해수내의 용존성 구리, 납, 아연 및 카드뮴의 농도는 각각 0.3∼1.75㎍/L, 0.013∼0.12㎍/L, 0.20∼6.14㎍/L, 0.007∼0.021㎍/L범위를 나타냈다. 퇴적물내의 구리, 납, 아연 및 카드뮴의 농도 12.0∼47.8㎍/g, 6.16∼59.5㎍/g, 43.0∼230㎍/g, 0.52∼11.2㎍/g 범위를 보였다. 굴의 구리, 납, 아연 및 카드뮴 농도는 12.1∼85.6㎍/g, 0.267∼1.48㎍/g, 1,070∼3,250㎍/g, 3.23∼7.71㎍/g 범위로 나타났다. 본 연구에서 임해공단 연안에 있는 굴의 중금속 농도는 Mussel Watch(1992) 조사와 비교해 볼 때 크게 오염되지 않은 것으로 사료된다. To investigate the degree of contamination from Korean coastal region, the concentrations of Cu, Pb, Zn and Cd in sediments, seawater and oyster(Crassostrea gigas) at Masan Bay, Onsan Bay, Daesan industrial complex and their control areas were analysed. Values for sediments, seawater and oyster in the industrial complex coastal region were higher than those in the control area except for seawater in Daesan. the values for dissolved phase of Cu, Pb, Zn and Cd in seawater showed 0.3∼1.75㎍/L, 0.013∼0.12㎍/L, 0.20∼6.14㎍/L and 0.007∼0.021㎍/L, respectively. The concentrations of Cu, Pb, Zn and Cd in sediments were 12.0∼47.8㎍/g, 6.16∼59.5㎍/g, 43.0∼230㎍/g and 0.52∼11.2㎍/g, respectively. The concentrations of Cu, Pb, Zn and Cd in oyster showed 12.1∼85.6㎍/g, 0.267∼1.48㎍/g, 1,070∼3,250㎍/g and 3.23∼7.71㎍/g, respectively. The contents of heavy metals in oysters at industrial complex coastal region showed that they were not seriously contaminated compared with those of Mussel Watch(1992).

EHP와 RRV의 Coinfection에 의한 Reassortants 선발에 관한 연구

유제현,조홍찬,송진욱,김유성,이영건,박선오,이종익,김응률,차광종,류영수,Harry B. Greenberg 건국대학교 동물자원연구센터 1998 動物資源硏究誌 Vol.19 No.-

본 연구는 두 종류의 rotavirus strain MA104 cell 상에서 동시감염시킨 후 gene이 서로 교차된 새로운 reassortants를 선발하기 위해 수행하였으며, 수행한 결과는 다음과 같다. 1. EHP T 와 RRV를 MA104 세포에 감염시켜 cytopathic effect(CPE)를 확인하였으며 확인된 virus를 수확하였다. 2. Coinfection의 mobility of infection은 각각 EHP T는 0.3(3*10?? PFU), RRV는 2.5(2.5*10?? PFU)이었다. 3. EHP T와 RRV를 coinfection하여 plaque assay에 의해 형성된 plaque들을 pick up하였다. 4. EHP T와 RRV를 동시감염시켜 얻어진 plaques를 MA104 cell에 재감염한 후 grow up하여 수확하였다. 5. Plaques를 24 Well의 MA104 cell에 감염하여 수확한 후 dsRNA를 추출하여 전기영동에 의해 26개 reassortants를 선발하였다. This study was carried out to select new reassortants whose genes were exchanged by confection of two strains. The results obtained in these experiments were as follows: 1. Cytopathic effect was checked out in the experiment in which made MA104 cells infected by EHP T (murine rotavirus) and RRV(simian rhesus rotavirus). And then the viruses were obtained from the procedure. 2. Mobility of infection(MOI) of EHP T and RRV were 0.3(3*10?? plaque forming units) and 2.5(2.5*10?? plaque forming units)respectively. 3. After coinfection of EHP T and RRV, plaques which formed in plaque assay were picked up. 4. Coinfected EHP T and RRV plaques were grown up in 24 well of MA104 cell and then grown-ups or those were obtained. 5. By polyacrylamide gel electrophoresis(PAGE) of dsRNA extracted above, 26 reassortants were selected.

치즈 제조 부산물을 이용한 ELISA Blocking Agent의 개발에 관한 연구

유제현,송진욱,조흥찬,차광종,박범석,김응률,이종익,이중복,谷口孝喜 건국대학교 동물자원연구센터 1999 動物資源硏究誌 Vol.20 No.-

본 연구는 유청과 탈지분유를 blotto로 이용하여 ELISA 수행시 항원·항체반응에 비특이반응을 막는 Blocking agent를 탐색하기 위해 실험을 수행했다. MAb의 역가측정 결과 유의차가 인정되는 OD값이 0.3이상 검색된 희석배율은 1B2의 경우 819,200배, 6D4의 경우 1,638,400배, 15B10의 경우 204,800배이었다. 서로 다른 Blotto가 항원·항체반응에 미치는 영향을 각기다른 MAb를 이용하여 조사해 본 결과 MAb Yo-156, Yo-5는 Blocking agent로 표준적으로 사용하고 있는 BAS가 비교하여 group A 바이러스 7종 모두에 비슷한 정도의 발색을 일으켰으며, MAb G6는 G6 type바이러스 4종 모두에서 비슷한 정도의 발색을 일으켰고, MAb G10은 G10 type 바이러스 3종 모두에서 비슷한 정도의 발색을 일으켰다. 따라서 본 실험에 사용한 모든 종류의 blotto소재인, 탈지분유1(서울우유(협)), 탈지분유2((주)매일유업), 혼합탈지분유((주)매일유업), whey powder1(Sigma), whey powder2 ((주)삼익유가공), WPC34((주)환이상사), WPC800((주)후드피아), WPC8000 ((주)후드피아), WPI((주)환이상사)는 현재 Blocking agent로 사용하고 있는 BSA와 비교하여 유사하게 비특이 반응을 막아주는 것으로 밝혀져 경제적으로 보다 저렴한 blotto 소재로 이용이 가능하다는 사실을 확인하였다. Enzyme-Linked-Immuno-Sorbent-Assay (ELISA) has been frequently used for detection of interaction of antigen and antibody. Many efforts have been made to reduce nonspecific reaction of ELISA. Bovine serum albumin (BSA) is usually used for blocking the nonspecific reaction even thought it is expensive. Recently skin milk has been focused on blocking nonspecific reaction of ELISA. This study aimed to substitute expensive blocking agent of ELISA with cheap by-product of cheese manufacture. To evaluate the substitution of BSA with wheys made in different companies, ELISA was carried out using monoclonal antibodies (MAbs) against rotavirus. The blocking capacity of the whey was compared with that of the BSA. The commercial wheys showed the same activity as BSA when ELISA was performed using MAb YO-156, S2-37, and Yo-5. To further investigate whether the whey affects the detection specificity of MAb, the ELISA was carried out using type specific MAbs. MAb G6 specific to serotype G6 bound to only rotavirus serotype G6 such as NCDV, UK, JBR, and Jedong strain whereas MAb G10 specific to serotype G10 bound to serotype G10 rotavirus only, demonstrating that the whey a and no effect on the binding specificity of MAb with respect to antigen-antibody reaction. These data indicate that the whey may have no effect on specific interation of antigen-antibody and may be widely used for blocking substitute because of competitive priority of its price.

Syntheses of PCDTBT containing tetrafluorobenzene as electron-withdrawing group with deep HOMO energy level and applications for photovoltaics

Kim, J.,Kim, N.H.,Song, S.,Park, S.Y.,Chae, S.,Bae, E.,Kim, I.,Kim, H.J.,Kim, J.Y.,Suh, H. IPC Science and Technology Press 2016 Polymer Vol.102 No.-

Conjugated polymers using tetrafluorophenylene unit were synthesized and deployed for photovoltaics. The tetrafluorophenylene unit which has the strong electron-withdrawing ability, was utilized for the copolymers of push-pull type with low bandgaps for organic solar cells. 9H-Carbazole as electron-rich unit and di-2-thienyl-2,1,3-benzothiadiazole (DTBT) or tetrafluorophenylene unit as electron-deficient unit were utilized for the syntheses of donor-acceptor (D-A) types of conjugated polymers with different ratios of tetrafluorophenylene unit (PCDTBTF-1, PCDTBTF-3 and PCDTBTF-10). By introduction of tetrafluorophenylene unit in the backbone of PCDTBT, the energy level of the highest occupied molecular orbital (HOMO) and the bandgap were decreased as compared to those of PCDTBT. The higher amount of tetrafluorophenylene unit, the lower energy levels of the HOMO of the polymers were observed. The device of PCDTBTF-1:PC<SUB>71</SUB>BM (1:1) with additive of diphenyl ether (DPE) showed an open-circuit voltage (V<SUB>OC</SUB>) of 0.82 V, a short circuit current (J<SUB>SC</SUB>) of 9.66 mA/cm<SUP>2</SUP>, and a fill factor (FF) of 0.44, which yields PCE of 3.44%.