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Kim, H.H.,Matthijnssens, J.,Kim, H.J.,Kwon, H.J.,Park, J.G.,Son, K.Y.,Ryu, E.H.,Kim, D.S.,Lee, W.S.,Kang, M.I.,Yang, D.K.,Hyun, B.H.,Park, S.I.,Park, S.J.,Cho, K.O. Elsevier Science 2012 INFECTION GENETICS AND EVOLUTION Vol.12 No.7
Group A rotaviruses (RVAs) are agents causing severe gastroenteritis in infants and young animals. G9 RVA strains are believed to have originated from pigs. However, this genotype has emerged as the fifth major human RVA genotype worldwide. To better understand the relationship between human and porcine RVA strains, complete RVA genome data are needed. For human RVA strains, the number of complete genome data have grown exponentially. However, there is still a lack of complete genome data on porcine RVA strains. Recently, G9 RVA strains have been identified as the third most important genotype in diarrheic pigs in South Korea in combinations with P[7] and P[23]. This study is the first report on complete genome analyses of 1 G9P[7] and 3 G9P[23] porcine RVA strains, resulting in the following genotype constellation: G9-P[7]/P[23]-I5-R1-C1-M1-A8-N1-T1-E1-H1. By comparisons of these genotype constellations, it was revealed that the Korean G9P[7] and G9P[23] RVA strains possessed a typical porcine RVA backbone, similar to other known porcine RVA strains. However, detailed phylogenetic analyses revealed the presence of intra-genotype reassortments among porcine RVA strains in South Korea. Thus, our data provide genetic information of G9 RVA strains increasingly detected in both humans and pigs, and will help to establish the role of pigs as a source or reservoir for novel human RVA strains.
Genetic diversity of the VP7, VP4 and VP6 genes of Korean porcine group C rotaviruses
Jeong, Y.J.,Matthijnssens, J.,Kim, D.S.,Kim, J.Y.,Alfajaro, M.M.,Park, J.G.,Hosmillo, M.,Son, K.Y.,Soliman, M.,Baek, Y.B.,Kwon, J.,Choi, J.S.,Kang, M.I.,Cho, K.O. Elsevier Scientific Pub. Co 2015 Veterinary microbiology Vol.176 No.1
Porcine group C rotaviruses (RVCs) are considered important pathogens due to their economic impact on pig industry and may also cross the host species barrier toward humans. Unlike RVA, however, genetic and phylogenetic data on RVCs from pigs and other host species are scarce. In the present study, full-length ORF sequences of 26 VP7, 9 VP4 and 9 VP6 genes of Korean porcine RVC strains were compared with those of other known RVC strains by phylogenetic analyses and pairwise identity frequency graphs. Applying the established 85% nucleotide identity cut-off value for RVC VP7 classification, the 26 Korean porcine RVC strains belonged to the G1, G3, G6 and G7 genotypes. Although more complete RVC VP4 sequences are warranted before a definitive cut-off value could be determined, a provisional 83% nucleotide cut-off value proposed for RVC VP4 classification resulted in 7 P-genotypes, 5 of which possessed porcine RVC strains. A 90% nucleotide cut-off value for VP6 divided RVC strains into 7 I-genotypes, 5 of which had porcine RVC strains. G/P/I-genotype comparisons suggested the occurrence of rather frequent reassortment events among Korean porcine RVC strains, and strong geographical differences in the distribution of RVC G-genotypes worldwide. Our data indicate that a large genetic diversity exists among porcine RVC strains. For the final genotype determination of each gene segment, more intensified epidemiological studies on animal and human RVC strains throughout the world are needed.
Lee, S. Y.,Choi, J. E.,Jeon, H. S.,Hong, M. J.,Choi, Y. Y.,Kang, H. G.,Yoo, S. S.,Lee, E. B.,Jeong, J. Y.,Lee, W. K.,Lee, J.,Cha, S. I.,Kim, C. H.,Kim, Y. T.,Jheon, S.,Son, J. W.,Park, J. Y. Oxford University Press 2015 ANNALS OF ONCOLOGY Vol.26 No.6
<P>In this study, <I>KRT81</I> rs3660G>C was associated with survival of patients with NSCLC after surgical resection. Mechanistic study suggested that the G-to-C change caused reduced binding efficiency of miRNA, leading to decreased translational repression, thereby increased <I>KRT81</I> expression. The <I>KRT81</I> rs3660G>C may be a useful prognostic biomarker in early-stage NSCLC patients.</P><P><B>Background</B></P><P>MicroRNAs (miRNAs) have a key role in carcinogenesis through negative regulation of their target genes. Therefore, genetic variations in miRNAs or their target sites may affect miRNA–mRNA interactions, thereby result in altered expression of target genes. This study was conducted to investigate the associations between single-nucleotide polymorphisms (SNP) located in the miRNA target sites (poly-miRTSs) and survival of patients with early-stage non-small-cell lung cancer (NSCLC).</P><P><B>Methods</B></P><P>Using public SNP database and miRNA target sites prediction program, 354 poly-miRTSs were selected for genotyping. Among these, 154 SNPs applicable to Sequenom's MassARRAY platform were investigated in 357 patients. A replication study was carried out on an independent patient population (<I>n</I> = 479). <I>Renilla</I> luciferase assay and reverse transcription-polymerase chain reaction were conducted to examine functional relevance of potentially functional poly-miRTSs.</P><P><B>Results</B></P><P>Of the 154 SNPs analyzed in a discovery set, 14 SNPs were significantly associated with survival outcomes. Among these, <I>KRT81</I> rs3660G>C was found to be associated with survival outcomes in the validation cohort. In the combined analysis, patients with the rs3660 GC + CC genotype had a significantly better overall survival compared with those with GG genotype [adjusted hazard ratio (aHR) for OS, 0.65; 95% confidence interval (CI) 0.50–0.85; <I>P</I> = 0.001]. An increased expression of the reporter gene for the C allele of rs3660 compared with the G allele was observed by luciferase assay. Consistently, the C allele was associated with higher relative expression level of <I>KRT81</I> in tumor tissues.</P><P><B>Conclusion</B></P><P>The rs3660G>C affects KRT81 expression and thus influences survival in early-stage NSCLC. The analysis of the rs3660G>C polymorphism may be useful to identify patients at high risk of a poor disease outcome.</P>
Do, K.H.,Choi, Y.W.,Kim, E.K.,Yun, S.J.,Kim, M.S.,Lee, S.Y.,Ha, J.M.,Kim, J.H.,Kim, C.D.,Son, B.G.,Kang, J.S.,Khan, I.A.,Bae, S.S. G. Fischer 2009 Phytomedicine Vol.16 No.6
Lignans are major constituents of plant extracts and have important pharmacological effects on mammalian cells. Here we showed that pinoresinol-4,4'-di-O-β-d-glucoside (PDG) from Valeriana officinalis induced calcium mobilization and cell migration through the activation of lysophosphatidic acid (LPA) receptor subtypes. Stimulation of mouse embryo fibroblast (MEF) cells with 10μM PDG resulted in strong stimulation of MEF cell migration and the EC<SUB>50</SUB> was about 2μM. Pretreatment with pertussis toxin (PTX), an inhibitor of G<SUB>i</SUB> protein, completely blocked PDG-induced cell migration demonstrating that PDG evokes MEF cell migration through the activation of the G<SUB>i</SUB>-coupled receptor. Furthermore, pretreatment of MEF cells with Ki16425 (10μM), which is a selective antagonist for LPA<SUB>1</SUB> and LPA<SUB>3</SUB> receptors, completely blocked PDG-induced cell migration. Likewise, PDG strongly induced calcium mobilization, which was also blocked by Ki16425 in a dose-dependent manner. Prior occupation of the LPA receptor with LPA itself completely blocked PDG-induced calcium mobilization. Finally, PDG-induced MEF cell migration was attenuated by pretreatment with a phosphatidylinositol 3-kinase (PI3K) inhibitor such as LY294002. Cells lacking downstream mediator of PI3K such as Akt1 and Akt2 (DKO cells) showed loss of PDG-induced migration. Re-expression of Akt1 (but not Akt2) completely restored PDG-induced DKO cell migration. Given these results, we conclude that PDG is a strong inducer of cell migration. We suggest that the pharmacological action of PDG may occur through the activation of an LPA receptor whereby activation of PI3K/Akt signaling pathway mediates PDG-induced MEF cell migration.
Yorkshire 수퇘지의 경제형질 및 선발지수에 대한 검정종료월 보정계수 개발
이정규,황선숙,손창준,박중양 한국동물자원과학회 2001 한국축산학회지 Vol.43 No.4
The present study was undertaken to evaluate environmental effects on average daily gain(ADG), backfat thickness and feed/gain and selection index and thereby to derive correction factors for above traits and index, using 5,048 Yorkshire boars that had been performance-tested from January, 1993, through December, 1999, at the Korea Swine Test Station. The results obtained were as follows; Each test year and month was called according to the last day of the performance test. 1. Effects of test year and test month were significant(P$lt;0.01) in all the traits that had been included in the present study. 2. The ADG and selection index increased each year, whereas backfat thickness decreased. Feed/gain, albeit significantly affected by the test year, did not exhibit any apparent temporal trend. 3. The ADG was greatest between March and June(942.45∼955.89g); backfat thickness was lowest during August, September and November(1.302∼1.314㎝); feed/gain was lowest during March, April and June(2.261∼2.272); and the selection index also was superior during March, April and June(225.07∼226.69) to those during the rest of the year. 4. The selected ratio was estimated to range from 0.782 for April to 0.801 for July when the traits and selection index were adjusted in an additive mode, whereas in a multiplicative adjustment mode, it ranged from 0.733(February) to 0.808(July). 5. By the additive mode analysis, the test month-adjusted ADG ranged from 885.79g(December) to 900.33g(August); backfat thickness from 1.332㎝(April) to 1.339(July and December); feed/gain from 2.320(April) to 2.346(September and October); and selection index from 216.94(October) to 219.09(March). 6. By the multiplicative mode analysis, the adjusted ADG ranged from 886.58g(December) to 903.65g(August); backfat thickness from 1.332㎝(April) to 1.339(July, September and December); feed/gain from 2.320(April) to 2.348(October); and selection index from 217.03(October) to 219.15(March). 7. Rank correlation coefficients between unadjusted and adjusted values were satisfactorily high in all the examined traits and index; they, after multiplicative and additive adjustments, respectively, were 0.903 and 0.900 in ADG, 0.987 and 0.987 in backfat thickness, 0.970 and 0.971 in feed/gain and 0.935 and 0.936 in selection index. 8. The rank correlation coefficient was 1.000 between the selection index which had been calculated using the additive mode-adjusted ADG, backfat thickness and feed/gain and that which had been one-step-adjusted by the additive mode.
Identification of Candidates for Early Discharge After Gastrectomy
Park, J. H.,Son, Y. G.,Kim, T. H.,Huh, Y. J.,Yang, J. Y.,Suh, Y. J.,Suh, Y. S.,Kong, S. H.,Lee, H. J.,Yang, H. K. Springer Science + Business Media 2017 Annals of Surgical Oncology Vol.24 No.1
<P>Patients younger than 68 years of age who underwent laparoscopic gastrectomy without combined resection might be candidates for early discharge on POD 5 if the POD 5 to preoperative WBC ratio is <= 1.2, POD 5 CRP level is <= 5.38 g/mL, and POD 4 body temperature is <= 37.4 degrees C.</P>
Jang, I. C.,Son, C. G.,Yang, S. M. G.,Lee, J. W.,Cho, A. R.,Aravindan, V.,Park, G. J.,Kang, K. S.,Kim, W. S.,Cho, W. I.,Lee, Y. S. Royal Society of Chemistry 2011 Journal of materials chemistry Vol.21 No.18
<P>LiCoPO<SUB>4</SUB> and Li<SUB>1.02</SUB>(Co<SUB>0.9</SUB>Fe<SUB>0.1</SUB>)<SUB>0.98</SUB>PO<SUB>4</SUB> were prepared by conventional solid state reactions. The surface modification of Li<SUB>1.02</SUB>(Co<SUB>0.9</SUB>Fe<SUB>0.1</SUB>)<SUB>0.98</SUB>PO<SUB>4</SUB> particulates by LiFePO<SUB>4</SUB> was successfully carried out by a dry coating procedure. TEM analysis confirmed the presence of a LiFePO<SUB>4</SUB> coating layer of about 20 nm on the surface of the Li<SUB>1.02</SUB>(Co<SUB>0.9</SUB>Fe<SUB>0.1</SUB>)<SUB>0.98</SUB>PO<SUB>4</SUB> particles. All three cells delivered high initial discharge capacities of 122, 130 and 128 mA h g<SUP>−1</SUP> for LiCoPO<SUB>4</SUB>, Li<SUB>1.02</SUB>(Co<SUB>0.9</SUB>Fe<SUB>0.1</SUB>)<SUB>0.98</SUB>PO<SUB>4</SUB>, and LiFePO<SUB>4</SUB> modified Li<SUB>1.02</SUB>(Co<SUB>0.9</SUB>Fe<SUB>0.1</SUB>)<SUB>0.98</SUB>PO<SUB>4</SUB>, respectively. However, these cells presented quite different cycle retention rates after 20 cycles, 21, 22 and 70% for LiCoPO<SUB>4</SUB>, Li<SUB>1.02</SUB>(Co<SUB>0.9</SUB>Fe<SUB>0.1</SUB>)<SUB>0.98</SUB>PO<SUB>4</SUB>, and LiFePO<SUB>4</SUB> modified Li<SUB>1.02</SUB>(Co<SUB>0.9</SUB>Fe<SUB>0.1</SUB>)<SUB>0.98</SUB>PO<SUB>4</SUB>, respectively. The improved cycle retention of the LiFePO<SUB>4</SUB>-modified Li<SUB>1.02</SUB>(Co<SUB>0.9</SUB>Fe<SUB>0.1</SUB>)<SUB>0.98</SUB>PO<SUB>4</SUB> resulted from its reduced reactivity towards the electrolyte and the effective prevention of resistive layer formation on the LiCoPO<SUB>4</SUB> surface during high voltage cycling.</P> <P>Graphic Abstract</P><P>LiFePO<SUB>4</SUB> modified Li<SUB>1.02</SUB>(Co<SUB>0.9</SUB>Fe<SUB>0.1</SUB>)<SUB>0.98</SUB>PO<SUB>4</SUB> cathodes resulted in improved cyclability, which is due to reduced reactivity towards electrolytes. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1jm10574d'> </P>
Kook, S-H.,Son, Y-O.,Choe, Y.,Kim, J-H.,Jeon, Y-M.,Heo, J-S.,Kim, J-G.,Lee, J-C. Blackwell Publishing Ltd 2009 Journal of periodontal research Vol.44 No.3
<P>Background and Objective: </P><P>The cellular response of human gingival fibroblasts to a mechanical force is considered to be primarily anti-osteoclastic because they produce relatively high levels of osteoprotegerin. However, there is little information available on the effects of compression force on the production of osteoprotegerin and osteoclastic differentiation by these cells. In this study, we examined how mechanical force affects the nature of human gingival fibroblasts to produce osteoprotegerin and inhibit osteoclastogenesis.</P><P>Material and Methods: </P><P>Human gingival fibroblasts were exposed to mechanical force by centrifugation for 90 min at a magnitude of approximately 50 g/cm<SUP>2</SUP>. The levels of osteoprotegerin, receptor activator of nuclear factor-&kgr;B ligand (RANKL), interleukin-1&bgr; and tumor necrosis factor-&agr; were measured at various time-points after applying the force. The effect of the centrifugal force on the formation of osteoclast-like cells was also determined using a co-culture system of human gingival fibroblasts and bone marrow cells.</P><P>Results: </P><P>Centrifugal force stimulated the expression of osteoprotegerin, RANKL, interleukin-1&bgr; and tumor necrosis factor-&agr; by the cells, and produced a relatively high osteoprotegerin to RANKL ratio at the protein level. Both interleukin-1&bgr; and tumor necrosis factor-&agr; accelerated the force-induced production of osteoprotegerin, which was inhibited significantly by the addition of anti-(interleukin-1&bgr;) immunoglobulin Ig isotype; IgG (rabbit polyclonal). However, the addition of anti-(tumor necrosis factor-&agr;) immunoglobulin Ig isotype; IgG1 (mouse monoclonal) had no effect. Centrifugal force also had an inhibitory effect on osteoclast formation.</P><P>Conclusion: </P><P>Application of centrifugal force to human gingival fibroblasts accelerates osteoprotegerin production by these cells, which stimulates the potential of human gingival fibroblasts to suppress osteoclastogenesis. Overall, human gingival fibroblasts might have natural defensive mechanisms to inhibit bone resorption induced by a mechanical stress.</P>
Developmental Efficiency of Bovine Embryos Cloned with Fetal Fibroblast Arrested at G0/G1 Phase
Cho S.R.,Son W.J.,Park C.S.,Park G.J.,S.Y. Choe 한국발생생물학회 2003 한국발생생물학회 학술발표대회 Vol.2003 No.1
The study evaluated the effect of donor cell treatments for G0/Gl synchronization and the donor ceil type on development and incidence of apoptosis in cloned cattle embryos. Primary cultures were established from a female fetus on day 50 of gestation and