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        Refolding with Simultaneous Purification of Recombinant Core Streptavidin Using Single-step High-performance Hydrophobic Interaction Chromatography

        Siyao Wang,Yuejuan Zhang,Dong Gao,Jing Zi,Wenpeng Wang,Nianzhe Zhang,Yi Wan,Lili Wang 한국생물공학회 2019 Biotechnology and Bioprocess Engineering Vol.24 No.4

        Streptavidin has applied to many areas including detection, purification, labeling, crosslinking and immobilization resulting in a high demand on its production. In this study, we report a method for preparation of recombinant core streptavidin (cSAV) protein using highperformance hydrophobic interaction chromatography (HPHIC). Firstly the cSAV was successfully cloned and expressed in Escherichia coli as inclusion bodies. A bifunctional stationary phase mainly working as HIC mode accompanied by weak anion exchange chromatography (WAX) was prepared using β-phenylethylamine (PEA) as a ligand. The denatured cSAV was then refolded and simultaneously purified by PEA hydrophobic interaction chromatography (PEA-HIC). The mass recovery and purity of cSAV by single-step were 30.2% and 98%, respectively. The bioactivity was determined to be 13.2 U/mg by biotin binding capacity assay. This method provides a new possibility for fast separation with simultaneous renaturation of cSAV.

      • KCI등재
      • KCI등재

        백범 김구의 문화국가 정치사상

        형사요(Siyao XING) 한국정치사회연구소 2023 한국과 국제사회 Vol.7 No.1

        이 연구는 백범 김구의 문화국가 정치사상에서 드러난 국제정치관을 분석함으로써 그의 사상적 특징을 밝히는 것을 목적으로 한다. 백범의 국제정치관에서 다른 국가에 대한 군사적 개입을 배제하는 불개입원칙은 평화지향적이고, 방어적인 군사가치관이 명확했으며, 국제 도의(道義)라는 도덕성을 기반으로 형성하는 협력적인 국제관계를 제시했다. 국가 간의 주권적, 정신적 평등에 대한 강조는 백범의 평등 사상을 구현했다. 세계 평화를 창출하고 대동세계(大同世界)를 실현하는 것은 문화국가가 추구하는 궁극적인 목표이다. 이에 대한 백범의 주장에서 평등, 호조주의(互助主義), 세계주의, 비군국주의, 평화지향성 등의 사상적 특징을 보여주었다. 문화국가를 통해 드러난 백범의 국제정치관은 높은 수준의 윤리와 도덕을 가진 국가 도덕성을 강조한다. 그의 이러한 사상은 무력과 폭력을 배제하고, 문화와 윤리 도덕으로 국가와 천하를 다스리는 왕도(王道)정치라는 유가(儒家)적 특징이 매우 뚜렷하게 드러났다. This study aims to reveal Kim Gu’s ideological characteristics by analyzing his perspective on international politics as revealed in his political thought on the cultural state. Grounded in his perspective on international politics, Kim’s non-intervention principle, which excludes military intervention in other countries, is clearly peace-oriented and imbued with defensive military values. Kim also favored cooperative international relations based on international morality. The emphasis on sovereign and spiritual equality between states was at the core of Kim’s idea of equality. The ultimate goals of a cultural state are creating world peace and realizing a cosmopolitan world. Such ideological characteristics as equality, cooperation, globalism, non-militarism, and peace-orientedness were touchstones of Kim Gu’s thought. Kim’s view of international politics as revealed through the cultural state emphasizes national morality with a high level of ethics and morality. His thinking clearly reflected the Confucian approach to politics grounded in the way of the true king, which excludes force and violence yet governs the state and the world through culture, ethics, and morality.

      • A comparative study of natural and ventilated supercavitation across two closed-wall water tunnel facilities

        Shao, Siyao,Karn, Ashish,Ahn, Byoung-Kwon,Arndt, Roger E.A.,Hong, Jiarong Elsevier 2017 Experimental thermal and fluid science Vol.88 No.-

        <P><B>Abstract</B></P> <P>Despite half a century of experimental investigation into both natural and ventilated supercavitation, there are still significant discrepancies among the results, in terms of supercavity geometry and ventilation demand, etc., under approximately similar conditions from different water tunnel facilities. To understand the influences of the flow facilities on the supercavitation experiments, a systematic comparison is conducted using the results from two closed-wall water tunnels, i.e. the Saint Anthony Falls high-speed water tunnel and the Chuangnam National University Closed Tunnel. For both ventilated and natural supercavitation, the experimental conditions from the two facilities are designed to match over a wide range of Froude number and blockage ratio, etc. For the ventilated supercavitation, the cavitation number for generating a ventilated supercavity and the hysteresis process for sustaining a supercavity show a proper match across the two facilities while holding the Froude number and blockage ratio constant. However, the ventilation demand to form a supercavity shows a noteworthy difference across the facilities even under the same Froude number and blockage ratio. Such a difference in the ventilation requirement is attributed to the mismatch of Reynolds number, the detailed geometry of the cavitator models as well as the test section which influences the pressure distribution along the span of the supercavity. Similarly, for natural supercavitation, both facilities yield a similar vaporous cavitation number for the supercavity formation under the same Froude number and blockage ratio, as well as similar choking behavior, i.e. cavitation number stays constant despite the decrease of test section pressure once a natural supercavity forms. The theoretical analysis of the choking phenomenon explains the trend of cavitation number under choking and its dependence on cavitator geometry, Froude number as well as the pressure loss in the water tunnel. A geometry comparison is conducted for both natural and ventilated supercavities in the two facilities under the same Froude number, blockage ratio and cavitation number. The comparison results show differences in the normalized cavity total length across different facilities as well as supercavity types despite the similarities in the supercavity maximum diameter and half-length. These differences were attributed to the variance in the pressure and flow distributions from the different facilities and across ventilated and natural supercavitation. The natural supercavities from the two facilities are further compared with the estimated natural supercavitation in unbounded flow under the same cavitation conditions. The comparison result highlights the limitation of the conventional theory in capturing the cavity geometries in actual experiments.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A systematic comparison of supercavitation is conducted across two water tunnel facilities. </LI> <LI> Mismatch of ventilation demand for supercavity formation occurs across the facilities. </LI> <LI> Both facilities show similar choking phenomena in natural supercavitation experiments. </LI> <LI> The overall geometry of supercavity differs across the facilities and supercavitation modes. </LI> <LI> Discrepancies are attributed to the difference in cavitators and test section pressure distribution across the facilities. </LI> </UL> </P>

      • KCI등재

        아시아인프라투자은행(AIIB) 가입 및 미가입 요인 분석: 한국, 영국, 미국, 일본 사례를 중심으로

        邢思遙(Siyao Xing),김현(Hyun Kim) 한국아시아학회 2020 아시아연구 Vol.23 No.4

        이 논문은 중국의 주도로 설립되어 2016년 1월에 공식 출범한 아시아인프라투자은행(AIIB)에 한국과 영국이 창립회원국으로 가입한 요인들과 미국과 일본이 현재까지 가입하지 않은 요인들을 분석하고 국제레짐이론의 시각에서 설명하는데 목적이 있다. 분석의 결과로서 첫째, 한국과 영국이 미국의 반대에도 불구하고 AIIB에 가입한 주요 요인은 신자유주의적 제도주의에서 설명하듯이 AIIB가입을 통해 경제적 상호 이익을 얻을 수 있다고 판단했기 때문이었고, 또한 신현실주의에서 예상하듯이 아시아 금융체제에서의 자국의 상대적 지위 상승에 대한 기대 요인이 동시에 작용하였기 때문이었다. 둘째, 미국이 AIIB에 가입하지 않은 근본적 요인은 신현실주의 시각에서 예상하듯이 World Bank, ADB, IMF 등 국제금융체제에서 미국이 향유하던 지배적인 영향력이 약화됨으로써 자국의 상대적 지위가 저하될 것을 우려하였기 때문이었다. 셋째, 일본이 가입하지 않은 주요 요인은 아시아 금융체제에서 자국이 주도하는 ADB의 상대적 영향력과 위상이 저하될 수 있다고 예상하였기 때문인데 이는 신현실주의 시각을 통해 적절히 설명이 된다. 반면에 신자유주의적 제도주의는 미국과 일본이 가입을 지속적으로 거부해온 요인들을 적절히 설명하지 못한다. This paper aims to analyze the factors for the Republic of Korea and the United Kingdom to join the Asian Infrastructure Investment Bank (AIIB), which was founded under the leadership of China and officially launched in January 2016, and the factors for the United States and Japan not to join it so far. And it explains them from the perspective of international regime theory. As a result of the analysis, first, the main reason for Korea and the United Kingdom to join the AIIB, despite opposition from the United States, was that they took into consideration mutual economic benefits resulting from their participation in the AIIB, as properly explained by neo-liberalism. Another reason was because they anticipated the rise of their relative influence and position in the Asian financial system, as expected by neo-realism. Second, the underlying factor for the United States not to join the AIIB was that its existing dominant influence in the World Bank, the Asian Development Bank, and the IMF would be so undermined that its relative position in the international financial system could be reduced. Third, the main reason for Japan not to join was that it expected the relative cost of undermining its leading position in ADB for the Asian financial system, as properly explained by neo-realism. Finally, neo-liberalism does not properly explain the reasons for the United States or Japanese refusal to accede to the AIIB.

      • KCI등재

        Eco-friendly Strategies for the Material and Fabrication of Wearable Sensors

        Yan Liu,Siyao Shang,Shuting Mo,Peng Wang,Hai Wang 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.8 No.4

        Wearable sensors are attracting great attentions due to their potential applications in human health monitoring and caring systems. The wide utilization of wearable electronics may cause great burden to the environment, due to the vast contaminants generated in their fabrication and after their usage. Consequently, great eff orts are devoted to the eco-friendly strategies for the material and fabrication in wearable sensors. Herein, recent advantages in developing wearable sensors with eco-friendly materials and green manufacturing approaches are reviewed. The functional materials with accessibility, recoverability and degradability have participated in the sensors as substrates, sensing elements and conductors. The fabrication strategies, from facile manual schemes to low-emission automated techniques are also introduced with their merits and demerits. Finally, the existing challenges and future opportunities in this field are summarized.

      • KCI등재

        The Effect of Professor's Individualized Consideration on the Personal Growth of Chinese Students Study Abroad in Korea : The Moderating Effect of Perceived School Support

        SangWoo Hahm,SiYao Sun 국제문화기술진흥원 2020 International Journal of Advanced Culture Technolo Vol.8 No.2

        In general, members of an organization want their own growth and development. As these members grow or develop, they feel satisfied and fulfilled in the process. Growth or development can motivate members to become more involved in their jobs. Therefore, these concepts are very important for organizations as well as individual members. Various elements of the organization affect the growth of its members. Leaders influence members to achieve organizational goals. Leaders can therefore be an important variable influencing the growth of members. Specific leadership informally develops the potential to identify and satisfy members' personal needs and to achieve higher performance. These leader behaviors motivate members and drive their growth. Thus, this leadership will have a significant impact on the growth of members. In addition, in this relationship, if members have perceived organizational support, they can further improve their expectations for growth. Therefore, support from leaders and organizations will have a positive impact on employee growth. This study highlights the importance of the growth of members of the organization. It demonstrates the moderating effects of leader's individualized consideration and perceived organizational support necessary elements for member growth. Through this process, the purpose and significance of the study are to identify the variables that affect the personal growth of members and explain how they can grow.

      • KCI등재

        중국 사회신용체계와 디지털 권위주의: 농민공 임금 미지급 사례를 중심으로

        이가용,XING SIYAO,정종필 경희대학교 사회과학연구원 2022 社會科學硏究 Vol.48 No.3

        This study suggests that China's Social Credit System could fill the institutional gaps and resolve rural migrant workers' issues. China's Social Credit System has been portrayed as a 'critical case' of digital authoritarianism, which explains the development of internet technologies leads to stronger authoritarian regimes. A perspective of digital authoritarianism contends that the Chinese government monitors and controls its citizens by exploiting an artificial intelligence technology, the so-called Social Credit System. However, we argue that explaining the Social Credit System only as a surveillance tool misunderstands the policy. China has struggled with several social problems provoked by insufficient laws and regulations, including Internet financial scams and labor safety. In order to resolve the issues and promote trust in Chinese society, the Chinese Government established the Social Credit System. This research introduces a case that the Social Credit System contributes to solving complicated issues rather than working as a surveillance system. Specifically, we analyze how the Social Credit System resolves the wage nonpayment issue of rural migrant workers(nongmingongs). Since the population of rural migrant workers is enormous, the unpaid wages issue could invoke severe social instability if it maintains. The Social Credit System collects information of firms that did not pay for rural migrants, establishes blacklists, and imposes sanctions on the blacklists. Those companies registered as blacklists are penalized on several activities, such as financial loans, governmental subsidies, and governmental procurement. As the punishments become larger than the economic benefits achieved from not paying employees, the companies voluntarily compensate rural migrant workers to secure social credit scores. This research suggests that the Social Credit System could promote social stability and protect citizens' interests when leaders use it effectively 본 연구는 중국의 사회신용체계가 사회 문제 해결에 기여한 사례를 소개하였다. 사회신용체계는 디지털 권위주의 이론의 핵심 사례로 자주 언급되고 있다. 디지털 권위주의 이론을 지지하는 학자들은 디지털 기술의 발전이 더 강력한 권위주의 체제를 창출하고 있다고주장하며 그 대표적인 예로 중국의 사회신용체계를 소개하고 있다. 즉, 중국이 사회신용체계를 이용하여 개인 삶의 일거수일투족을 감시하고 통제한다는 것이다. 하지만 사회신용체계를 단순히 감시 도구로만 보는 시각은 한계가 있다. 중국은 미흡한 법 제도로 인하여금융 사기 등의 사회 문제를 겪고 있고, 이와 같은 문제를 해결하고 사회적 신뢰를 증진하기 위해서 사회신용체계를 고안해냈기 때문이다. 사회신용체계가 실제로 문제 해결에 어떻게 기여하는지 살펴보기 위하여 본 논문은 농민공 임금 미지급 문제를 분석하였다. 중국농민공은 인구 규모가 크기 때문에 임금 미지급 문제가 지속될 시 심각한 사회 문제로 발전할 가능성이 있음에도 불구하고 법 제도의 미흡함과 복잡한 절차 등으로 인하여 문제해결에 어려움을 겪고 있다. 중국은 사회신용체계를 통해 농민공의 임금을 미지급한 기업의 정보를 수집하고, 블랙리스트를 제정하여, 블랙리스트에 등재된 기업에 융자 대출, 정부 지원 보조금, 정부 조달 등 다양한 영역에서 제재를 가하였다. 농민공 임금 미지급으로 얻는 금전적 이익보다 불이익이 더 커지자 기업은 자발적으로 농민공의 임금을 지급하고신용 점수를 지키고자 노력하였다. 이와 같이 본 연구는 사회신용체계가 효과적으로 사용될 시, 중국 내의 고질적인 사회 문제를 해결하고 시민의 이익을 보호하는 용도로도 사용될 수 있다는 것을 밝히고자 한다.

      • KCI등재

        An experimental investigation of artificial supercavitation generated by air injection behind disk-shaped cavitators

        안병권,정소원,김지혜,Siyao Shao,Jiarong Hong,Roger E.A. Arndt 대한조선학회 2017 International Journal of Naval Architecture and Oc Vol.9 No.2

        In this paper, we investigated physical characteristics of an artificial supercavity generated behind an axisymmetric cavitator. Experiments for the same model were carried out at two different cavitation tunnels of the Chungnam National University and the University of Minnesota, and the results were compared and verified with each other. We measured pressures inside the cavity and observed the cavity formation by using a high-speed camera. Cavitation parameters were evaluated in considering blockage effects of the tunnel, and gravitational effects on supercavity dimensions were examined. Cavity dimensions corresponding to the unbounded cavitation number were compared. In addition, we investigated how artificial supercavitation develops according to the combination of injection positions and direction.

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