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Siriporn Chamniansawat,Sukumal Chongthammakun 생화학분자생물학회 2012 Experimental and molecular medicine Vol.44 No.6
The localization of estrogen (E2) has been clearly shown in hippocampus, called local hippocampal E2. It enhanced neuronal synaptic plasticity and protected neuron form cerebral ischemia, similar to those effects of exogenous E2. However, the interactive function of hippocampal and exogenous E2 on synaptic plasticity activation and neuroprotection is still elusive. By using hippocampal H19-7 cells, we demonstrated the local hippocampal E2 that totally suppressed by aromatase inhibitor anastrozole. Anastrozole also suppressed estrogen receptor (ER)β, but not ERα, expression. Specific agonist of ERα (PPT) and ERβ (DPN) restored ERβ expression in anastrozole-treated cells. In combinatorial treatment with anastrozole and phosphoinositide kinase-3 (PI-3K) signaling inhibitor wortmannin,PPT could not improve hippocampal ERβ expression. On the other hand, DPN induced basal ERβ translocalization into nucleus of anastrozole-treated cells. Exogenous E2 increased synaptic plasticity markers expression in H19-7 cells. However, exogenous E2could not enhance synaptic plasticity in anastrozoletreated group. Exogenous E2 also increased cell viability and B-cell lymphoma 2 (Bcl2) expression in H2O2-treated cells. In combined treatment of anastrozole and H2O2, exogenous E2 failed to enhance cell viability and Bcl2 expression in hippocampal H19-7cells. Our results provided the evidence of the priming role of local hippocampal E2 on exogenous E2-enhanced synaptic plasticity and viability of hippocampal neurons.
Hydrolysis of raw hide using proteolytic enzyme extracted from papaya latex
Siriporn Damrongsakkul,Sittiruk Pitpreecha 한국화학공학회 2006 Korean Journal of Chemical Engineering Vol.23 No.6
proteolytic enzyme was extracted from papaya latex using two solvents, water and phosphate bufferthe solvent extraction, the extracted enzyme was precipitated in 45 wt% saturated amonium sulfate solution. Theyield and activity of precipitated enzyme considerably decreased. Crude proteolytic enzyme extracted using water asan extracting liquid was, therefore, selected to use in gelatin production from raw hide hydrolysis, comparing to theuse of comercial papain. The effects of hydrolysis conditions on gelatin recovery and properties of obtained gelatinwere investigated. The optimum conditions for the activities of both crude extracted enzyme and comercial papainwere at 75oC and pH 7. At this condition, the highest percentages of gelatin recovery were obtained from raw hidemercial papain hydrolysis were similar. This proved that crude extracted enzyme from papaya latex could be effec-tively used in gelatin production, instead of the use of commercial papain, with a comparatively low cost.
Siriporn Tuntipopipat,Channarong Muangnoi,Mark L. Failla 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.6
Nitric oxide (NO) and tumor necrosis factor-α (TNF-α) play important roles in inflammatory processes. This study examined whether 13 spices/herbs commonly used in Thai dishes modulate the production of NO and TNF-α by the RAW 264.7 mouse macrophage cell line pretreated with plant extracts (1–100μg/mL) prior to activation by bacterial lipopolysaccharide (LPS). Tested plant tissues were extracted with ethanol with the exception of roselle, which was extracted with 70% acetone. Eight of the 13 plant extracts inhibited NO and TNF-α production in a dose-dependent manner without exerting cytotoxicity. Extract from Limnophila aromatica (Kyeng) was the most robust suppressor of NO production, followed by dill, kaffer lime, chili, Teaw, mint, sweet basil, and pea eggplant, respectively (range of 50% inhibitory concentration [IC50]=11.4–74.6μg/mL). Kyeng also exhibited the greatest inhibition of TNF-α production (IC50=10.5μg/mL). IC50 values for NO and TNF-α production in LPS-activated RAW 264.7 cells for these extracts were highly correlated (r=0.772, P=.025). These results suggest that extracts from some spices/herbs in the habitual Thai diet possess anti-inflammatory activity. Moreover, the results support the use of NO production in LPS-activated RAW 264.7 cells as a rapid and cost-effective tool for screening the anti-inflammatory activity of extracts of spices/herbs.
University and industry collaboration in Japan and Thailand: influence of university type
Siriporn Pittayasophon,Patarapong Intarakumnerd 기술경영경제학회 2017 ASIAN JOURNAL OF TECHNOLOGY INNOVATION Vol.25 No.1
This paper aims at investigating influence of university types on both modes and size ofcollaborating firm. The remarkable findings could be concluded as follows: (1) Universitytypes relate to modes in a certain degree. National universities tend to have R&Dcollaboration with firms, while other types of university do not predominantly rely on anyspecific mode. Not only government policy, historical background does matter as well. (2) Instead of university type, types of modes are likely to relate to the size of collaboratingfirm. Large firms tend to be potential R&D partners, whereas small and medium enterprises(SMEs) are focused partners for consulation. (3) Socio-economic characteristics of thelocation where universities are located affect roles of universities in supporting industry. National universities in local area often work with both large firms and SMEs in order torespond to local demands. In addition, universities’ specialisations perhaps influenceuniversity and industry collaboration. Different types of universities having relatively highspecialisation in engineering courses have similar roles in supporting industry and(4) country contexts influence roles of universities due to different policy focuses, anddifferent levels of universities and firms’ technological capabilities. Japanese university –industry collaboration seem to focus more on R&D acitivities, comparing to Thai universities.
Application of biomechanics to bone health
( Siriporn Sasimontonkul ) 한국체육학회 2016 국제스포츠과학 학술대회 Vol.2016 No.1
Impact activities are essential for triggering bone remodeling process throughout life. However, an optimal magnitude of impact loading to activate bone remodeling resulting in bone gain, without causing microscopic injuries of the bone, has not been explored. We found that fast running could increase bone mineral density (BMD) of young women’s tibia. It inferred that the magnitude of impact loads resulted from foot impact could activate osteocytes and bone remodeling in the tibia sequentially. Nevertheless, BMD of lumbar spine was unaltered. It was possible that the shock waves from impact loading were attenuated, by soft tissue of lower extremities, while they propagated from lower limb up to the trunk. Moreover, the frequency of shock wave at the lumbar was also reduced compared with that at the shank (Figure 1). Figure 1. Power spectral density (PSD) of acceleration measured at the shank (Run) and at the lumbar (RunWaist) In contrast to young bone, bone of the elders may not withstand, and get injured from, the impact loading. Our finite element analysis showed that the impact force occurred from fast running could induce the occurrence of large stresses inside elderly tibia (fig 2). However, the peak stresses are depended on bone contact area, bone shape and bone mass. Figure 2. Von-Misses stress distributed on the tibial surface
Siriporn Chaikaew,Sasitorn Baipong,Teruo Sone,Apinun Kanpiengjai,Naradorn Chui-chai,Kozo Asano,Chartchai Khanongnuch 한국미생물학회 2017 The journal of microbiology Vol.55 No.9
The microbiota of lactic acid bacteria (LAB) in thirty-five samples of Miang, a traditional fermented tea leaf product, collected from twenty-two different regions of eight provinces in upper northern Thailand was revealed through the culture-dependent technique. A total of 311 presumptive LAB strains were isolated and subjected to clustering analysis based on repetitive genomic element-PCR (rep-PCR) fingerprinting profiles. The majority of the strains belonged to the Lactobacillus genera with an overwhelming predominance of the Lb. plantarum group. Further studies of species-specific PCR showed that 201 of 252 isolates in the Lb. plantarum group were Lb. plantarum which were thus considered as the predominant LAB in Miang, while the other 51 isolates belonged to Lb. pentosus. In contrast to Lb. plantarum, there is a lack of information on the tannase gene and the tea tannin-tolerant ability of Lb. pentosus. Of the 51 Lb. pentosus isolates, 33 were found to harbor the genes encoding tannase and shared 93-99% amino acid identity with tannase obtained from Lb. pentosus ATCC 8041T. Among 33 tannase gene-positive isolates, 23 isolates exhibited high tannin- tolerant capabilities when cultivated on de Man Rogosa and Sharpe agar-containing bromocresol purple (0.02 g/L, MRS-BCP) supplemented with 20% (v/v) crude tea extract, which corresponded to 2.5% (w/v) tannins. These Lb. pentosus isolates with high tannin-tolerant capacity are expected to be the high potential strains for functional tannase production involved in Miang fermentation as they will bring about certain benefits and could be used to improve the fermentation of tea products.