Metabolic engineering in E. coli for production of C-glycosylated flavones and their physio-chemical studies.

Shrestha,DipeshDhakal,RameshPrasadPandey,Jae Kyung Sohng 한국당과학회 2018 한국당과학회 학술대회 Vol.2018 No.07

Effect of Daylength on the Panicle Exsertion of Panicle Enclosing Gamadi Rice Cultivar

Shrestha,G,L,M,H,Heu,S,Z,Park 한국작물학회 1981 한국작물학회지 Vol.26 No.2

수도의 묘대일수가 절간신장에 미치는 영향

Gyan L. Shrestha갼 랄 슈레스터 韓國作物學會 1985 Korean journal of crop science Vol.30 No.2

네팔에서 보급단계로 진전된 수도 3계통 IET 7251, BG 400-1 및 B 44 b-50-2-2-5-1 을 6월 5일 파종하여 10일, 20일, 30일, 40일, 50일 및 60일 묘를 이앙하고 그들의 출수일수와 절간신장을 조사하여 다음과 같은 결과를 얻었다. 1. 간장은 30일묘 이앙구에서 가장 길었고 그보다 묘대일수가 길어짐에 따라 현저하게 간장이 단축되었다. 2. 육면으로 감별이 가능한 절수도 30일묘 이앙구에서 가장 많았는데 40일묘까지는 큰 차가 없었으나 그보다 묘대기간이 길어지면 유의하게 절수가 감소 되었다. 3. 간장은 40일묘 이앙구에서 가장 길었다. 4. 출수 및 성숙까지의 일수는 이앙이 늦어질수록 증가되었다. Three different improved pre-release rice varieties, when transplanted at different ages of seedlings, showed that 40 day old seedlings produced the longest panicle, whereas 30 day old seedlings produced the highest culm length among treatments in all treated varieties. 40 day or older seedlings reduced the culm length remarkably mainly due to decrease in lower internodes in IET7251 and BG400-1, and due to upper as well as lower internodes in B44b-50-2-2-5-1. 30 day old seedlings produced maximum number of visible internodes. Heading as weil as maturity was delayed with increasing age of seedling.

In vitro에서 큰번데기 동충하초 자실체형성의 불안정성

부샨 쓰레스타 외 한국버섯학회 2004 한국버섯학회지 Vol.2 No.3

Traditionally, Cordyceps species have been used as a part of herbal medicine in Oriental countries, including Korea for internal health, vigor and to cure different diseases related to heart, lung etc. In recent years, research on artificial fruiting of some species of the genus Cordyceps including C. militaris has been carried out extensively because of their medicinal value. Instability observed in the in vitro fruiting of C. militaris is reported in the present study. 전통적으로 Cordyceps종은 한국을 포함한 동양에서 건강증진과 심장과 폐에 관련된 질환을 치료하는 약용식물의 하나로 사용되어왔다. 최근에 동충하초의 약리적 가치 때문에 큰번데기 동충하초를 포함한 몇가지 Cordyceps종의 인공재배에 관한 연구가 활발하게 이루어지고 있다. 본 연구에서는 큰번데기 동충하초를 in vitro에서 인공재배한 결과 자실체 형성이 불안정한 것을 관찰하였기에 보고하고자 한다.

Assessment on Scaling-Up of Mini-Grid Initiative: Case Study of Mini-Grid in Rural Nepal

Pramish Shrestha,Ashish Shrestha,Namrata Tusuju Shrestha,Antonis Papadakis,Ramesh Kumar Maskey 한국정밀공학회 2021 International Journal of Precision Engineering and Vol.8 No.1

A majority of Nepalese population are living in rural areas, where the electricity access by grid extension is not feasible. Development of micro-hydropower in the isolated state is one of the solutions to provide the electricity to these areas. However, the micro-hydro systems are facing numerous challenges, such as high investment cost, low reliability, low load factor and protection issues. For this reason, the concept of mini-grid has been developed to address the discussed challenges. In this study, a case study of a mini-grid, which is formed by interconnecting six isolated Micro Hydropower Plants (MHPs) with an installed capacity of 107 kW at a transmission voltage of 11 kV, is considered. This study performs the technical and financial analysis, to study the possibility for scaling up the system. Based on different factors, optimum models have been selected, considering a numbers of different cases including cost–benefit and sensitivity analysis. The mini-grid connection of several isolated MHPs is technically possible, but the financial feasibility depends upon various factors, such as the distance between the MHPs, end-use promotion, total capacity of MHPs, future load growth, promotion mechanisms, power trading with utility, etc. The findings show that sustainable operation of mini-grid requires the local capacity building, coordination, and understanding among community cooperatives.

δ-Catenin Increases the Stability of EGFR by Decreasing c-Cbl Interaction and Enhances EGFR/Erk1/2 Signaling in Prostate Cancer

Shrestha, Nensi,Shrestha, Hridaya,Ryu, Taeyong,Kim, Hangun,Simkhada, Shishli,Cho, Young-Chang,Park, So-Yeon,Cho, Sayeon,Lee, Kwang-Youl,Lee, Jae-Hyuk,Kim, Kwonseop Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.4

${\delta}$-Catenin, a member of the p120-catenin subfamily of armadillo proteins, reportedly increases during the late stage of prostate cancer. Our previous study demonstrates that ${\delta}$-catenin increases the stability of EGFR in prostate cancer cell lines. However, the molecular mechanism behind ${\delta}$-catenin-mediated enhanced stability of EGFR was not explored. In this study, we hypothesized that ${\delta}$-catenin enhances the protein stability of EGFR by inhibiting its lysosomal degradation that is mediated by c-casitas b-lineage lymphoma (c-Cbl), a RING domain E3 ligase. c-Cbl monoubiquitinates EGFR and thus facilitates its internalization, followed by lysosomal degradation. We observed that ${\delta}$-catenin plays a key role in EGFR stability and downstream signaling. ${\delta}$-Catenin competes with c-Cbl for EGFR binding, which results in a reduction of binding between c-Cbl and EGFR and thus decreases the ubiquitination of EGFR. This in turn increases the expression of membrane bound EGFR and enhances EGFR/Erk1/2 signaling. Our findings add a new perspective on the role of ${\delta}$-catenin in enhancing EGFR/Erk1/2 signaling-mediated prostate cancer.

PE-163: Association Liver Enzymes with Blood Pressure in Diabetic Patients

( Shrestha Rojeet ),( Shrestha Nirajan ),( Bhatt Nirmal Prasad ),( Dahal Sudimna ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Aims: Gamma-glutamytransferase (GGT) is routinely used laboratory investigation for liver damage and alcohol consumption. Serum GGT concentration is determined by body fat content, lipid and glucose levels, alcohol consumption and various medications. Several lines of evidence show that serum GGT is associated with cardiovascular and all-case mortality. It is also a marker of oxidative stress. Although serum GGT is associated with hypertension, the correlation between GGT and blood pressure has not been well established. The aim of the present study was to investigate the association between serum GGT and blood pressure in type 2 diabetic patients (T2DM). Methods: A total number of 105 patients with T2DM, who attended Nepal Medical College Teaching Hospital, Kathmandu, Nepal, were recruited for the study. There were 39 males (mean age: 56.6±5.2 years) and 66 females (mean age: 49.6±6.3). The patients with history of liver disease and alcohol intake were excluded from the study. After basic anthropometric measurement, fasting venous blood was collected and subjected for the estimation of liver enzymes. All data were expressed as mean± SD. p-values less than 0.05 were considered as statistically significant. Results: Although serum ALT and AST levels were significantly elevated within the normal range in T2DM compared to control, they are not correlated with systolic blood pressure (SBP) (r= 0.07 and 0.128, respectively) and diastolic blood pressure (DBP) (r= 0.08 and 0.02, respectively) in both men and women. However, serum GGT levels within their normal range were positively correlated with SBP (r=0.4, p<0.01) and DBP (r=0.275, p<0.05) in women. Interestingly, neither of these liver enzymes was correlated with blood pressure among men diabetic patients. Conclusions: These findings suggest that GGT may be linked with blood pressure balance in women and evaluating the levels of GGT could help in the monitoring of hypertension in diabetic individuals.

A conducting neural interface of polyurethane/silk-<i>f</i>unctionalized multiwall carbon nanotubes with enhanced mechanical strength for neuroregeneration

Shrestha, Sita,Shrestha, Bishnu Kumar,Lee, Joshua,Joong, Oh Kwang,Kim, Beom-Su,Park, Chan Hee,Kim, Cheol Sang Elsevier S.A. 2019 Materials Science and Engineering C Vol. No.

<P><B>Abstract</B></P> <P>A fibrous scaffold, fully assimilating polyurethane (PU) and silk fibroin associated with functionalized multi-walled carbon nanotubes (<I>f</I>MWCNTs) was developed by electrospinning technique. Herein, we engineered the PU/Silk fibroin-<I>f</I>MWCNTs-based biomaterial that shows great promise as electrospun scaffolds for neuronal growth and differentiation, because of its unique mechanical properties, hydrophilicity, and biodegradability, with outstanding biocompatibility in nerve tissue engineering. The morphology and structural properties of the scaffolds were studied using various techniques. In particular, the presence of <I>f</I>MWCNTs enhances the electrical conductivity and plausible absorption of sufficient extracellular matrix (ECM). The <I>in vitro</I> tests revealed that the aligned scaffolds (PU/Silk-<I>f</I>MWCNTs) significantly stimulated the growth and proliferation of Schwann cells (S42), together with the differentiation and spontaneous neurite outgrowth of rat pheochromocytoma (PC12) cells that were particularly guided along the axis of fiber alignment. The conductive PU/Silk-<I>f</I>MWCNTs scaffold significantly improves neural expression <I>in vitro</I> with successful axonal regrowth, which was confirmed by immunocytochemistry and qRT-PCR analysis. Inspired by the comprehensive experimental results, the <I>f</I>MWCNTs-based scaffold affords new insight into nerve-guided conduit design from both conductive and protein rich standpoints, and opens a new perspective on peripheral nerve restoration in preclinical applications.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Polyurethane-silk/multiwall carbon nanotubes based aligned electrospun scaffold was fabricated. </LI> <LI> A protein rich biomaterial showed high mechanical strength and good electrical conductivity. </LI> <LI> PC12 cells are well proliferated and differentiated on scaffold along with fibers orientation. </LI> <LI> The scaffold exhibited strong bioactivity, suited for <I>in vitro</I> nerve cell regeneration. </LI> </UL> </P>

π-Conjugated polyaniline-assisted flexible titania nanotubes with controlled surface morphology as regenerative medicine in nerve cell growth

Shrestha, Bishnu Kumar,Shrestha, Sita,Baral, Ek Raj,Lee, Ji Yeon,Kim, Beom-Su,Park, Chan Hee,Kim, Cheol Sang Elsevier 2019 CHEMICAL ENGINEERING JOURNAL -LAUSANNE- Vol.360 No.-

<P><B>Abstract</B></P> <P>Biologically active conjugated polymers, for example polyaniline (PANI), have drawn attention as emerging materials for applications in bio-medical implant devices, due to their inherent abilities with regard to charge-carrier properties, and their ability to immobilize biomolecules or proteins. Herein, we report an electrocoating of PANI on titania nanotubes (TNTs) via electrochemical oxidation of aniline with PANI layers of appropriate thickness (∼274 nm). Uniform titanium oxide nanotubes were first developed from titanium (Ti) foil through an anodization process, followed by calcination to obtain high purity TNTs vertically aligned on a Ti substrate. These had a large surface area, controllable tube height and diameter, and were highly biocompatible, and doping with PANI further improved their properties, like being antibacterial, having a lower charge transfer resistance (22.51 Ω) and strong anti-corrosion behavior (<I>E<SUB>corr</SUB> </I> ∼ − 184 mV, <I>I<SUB>corr</SUB> </I> ∼ 9.7 × 10<SUP>−7</SUP> Amp). <I>In vitro</I> experiments revealed that the cellular functions of PC12 and S42 cells on TNTs-PANI scaffolds show characteristic improvement in proliferation and differentiation owning to approach neuronal cells activation associated with axonal growth and migration in the peripheral nervous system (PNS). Thus, the flexible bioactive substrate is capable of stimulating neuronal cells, and can inspire neural transduction through direct neural interfaces.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A highly conductive electroactive polyaniline (PANI) was electrodeposited uniformly on titania nanotubes (TNTs) substrate. </LI> <LI> Titania nanotubes-polyaniline (TNTs-PANI) showed antibacterial activity and corrosion resistivity. </LI> <LI> The biointerface TNTs-PANI was used as basal substrate for PC12 cells regeneration and differentiation. </LI> <LI> The flexible TNTs-PANI substrate accelerated the neurites outgrowth of PC12 cells. </LI> </UL> </P>

Effect of Gibberellic Acid (GA₃) Treated at Various Growth Stages on Internode Contraction and Gamadiness in Rice

Shrestha, G.L.,Heu, M.H. 서울大學校 農科大學 1984 서울대농학연구지 Vol.9 No.2

GA₃처리에 依하여 나타나는 苞內成熟性品種 Gamadi의 節間短縮性이 生育단계別 GA₃처리로 어떻게 變化하는가를 검토하기 위하여 Japonica短稈品種이며 生體內 GA₃合成이 되지 않는 것으로 알려진 Tan-ginbozu와 보통 長稈品種인 Indica의 Pokhareli Mashino 그리고 Gamadi 品種을 發根期, 分얼中期, 幼穗分化期 및 穗孕期에 GA₃처리를 하였다. 그 結果는 다음과 같이 要約된다. 1. 처리에 依한 草丈伸長效果는 分얼中期와 幼穗分化期에서 가장 현저하였고 穗孕期에는 모든 品種에서 效果가 減少되었다. 生育初期의 처리效果는 Gamadi 品種에서 가장 微微하였고 Tan-ginbozu 品種에서 가장 현저하였다. 2. 草丈 伸長效果는 分얼中期에 처리한 것과 그 以後에 1回, 2回, 3回, 4回 처리한 것 들 間에 큰 差가 없었다. 3. 節間短縮에 미친 처리의 영향은 品種과 처리시기에 따라 다르게 나타났다. Tan-ginbozu는 發根期 처리로 稈長全體가 短縮되었고 그 以後의 처리에서는 上位節間들이 伸長되어 全體的으로 長稈이 되었다. Pokhareli Mashine도 上位節間이 伸長되어 全體的으로 長稈이 되었다. Gamadi는 下位節間들의 伸長으로 全體的으로 長稈이 되었지만 幼穗分化期처리로 苞內成숙성과 第3節間의 極端的인 短縮이 이뤄지고 그 以後의 처리로는 穗梗이 若于 伸長되었다. GA₃was treated on the rice cultivars Tan-ginbozu which is a dwarf Japonica, Pakhareli-Mashino which is a tall Indica, and the Gamadi is an Indica and their panicles do not exsert and remain in the sheath of flag leaf, at the growth stages of rooting, mid tillering panicle initiation and booting, to make clear about the influence of growth stages on the internode contraction of Gamadi cultivar by the treatment of GA₃. The results are summarized as follows; 1. The immediate effect of GA₃treatment was most pronounced at mid-tillering and panicle initiation stages and at booting stage it was reduced. At the rooting stage, Gamadi showed least effect and Tanginbozu showed most significant. 2. After mid-tillering stage. the immediate GA₃effect was not different significantly among treatments. 3. GA₃effects on the internode contraction was variable depending on the cultivar and the growth stages treated. Tan ginbozu treated at the rooting stage showed reduction in culm length, but increased it through elongation of upper internodes in other growth stages. Pokhareli-Mashino also increased the culm length the elongation of upper internodes. The Gamadi increased their culm length through elongation of lower internodes, contracted their 1st and 3rd internodes extreamly by the GA₃ treatment at the panicle initiation stage, and showed symptoms of peduncle elongation by the treatment at later growth stage.