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RISS 인기검색어
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- 저자 : Shovan Kumar Majumder (검색결과 1 건)
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Khan Mohammad Khan,Ragesh Kumar,Hemant Krishna,Nirmalya Ghosh,Shovan Kumar Majumder 대한의용생체공학회 2016 Biomedical Engineering Letters (BMEL) Vol.6 No.4
Purpose To report development of a depth-sensitivefluorescence spectroscopy system based on the configurationof point collection fixed at the centre of an illumination ringon the surface of a target sample. Methods The system makes use of an axicon for convertingthe collimated laser beam into ring shaped illumination andachieves the ability of subsurface interrogation by varyingthe radii of the illumination rings thereby introducing spatialoffsets between collection and illumination. The system wasvalidated on a non-biological phantom comprising a thin tissuepaper overlaying a thick dye card and also on a biologicaltissue sample that was a chicken leg tissue consisting of athin epithelial membrane on the top of a much thicker muscletissue. Results It was found that while the fluorescence spectracorresponding to zero spatial offset are dominated by thefluorescence emission bands associated with the top layer ofthe layered samples, the fluorescence spectra measured withspatial offsets contain higher contribution of fluorescencesignatures characteristics of the bottom layer as compared tothe spectrum measured with the zero offset. Conclusions The SOFS based system is able to interrogatesubsurface depths beyond the reach of the conventionalconfocal fluorescence by simply varying the position ofthe axicon in the excitation arm. Due to the simplicity inits instrumentation, the approach has the potential to bedeveloped into a portable system for in situ measurement ina tissue.
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