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Safavi, M.S.,Shojaosadati, S.A.,Dorkoosh, F.A.,Jo, H.J.,Kwon, Y.,Lee, K.C.,Yang, H.G.,Park, E.J.,Na, D.H. Association de pharmacie galénique industri 2017 Journal of drug delivery science and technology Vol.41 No.-
The aim of this study was to develop mechanical homogenization processes to fabricate protein-based nanoparticles. The high-pressure homogenizer (HPH) and high-speed homogenizer (HSH); were used to encapsulate the hydrophobic drug, tamoxifen, in albumin nanoparticles. The results revealed that the rotational speed with HSH and the pressure with HPH were the main factors affecting the size, while increasing the residence time led to more homogenous nanoparticles. Seven homogenization cycles at 14917 psi and 8.24 min of mixing at 17360 rpm ensured a drug loading of 14.2 +/- 1.9% and 11.6 +/- 2.3% for HPH and HSH, respectively. We found a direct correlation between the obtained size and energy input and retention time with both homogenizing devices. The characteristics of the optimized nanoparticles were within the desired range to meet the requirements of intravenous injection. The surface morphology of the nanoparticles determined by transmission electron microscopy showed semi-spherical nanoparticle shapes. Further, the secondary structure of albumin in nanoparticles was determined via circular dichroism, which showed only slight structural changes versus native albumin, making it a promising, self-targeted drug delivery system. Finally, BT474 viability assays and western blot analysis showed the effectiveness of the tamoxifen-loaded albumin nanoparticles prepared via homogenization.
M. Hashemi,S.A. Shojaosadati,S.H. Razavi,S.M. Mousavi 한국공업화학회 2015 Journal of Industrial and Engineering Chemistry Vol.21 No.1
The impact of nitrogen substrate on the a-amylase performance at two assay conditions (pH = 4.5, 65 8C;pH = 6.8, 55 8C) was investigated. The highest a-amylase activity difference ( 38%) was related to themedium consist of dextrin, meat and yeast extracts at ( 1) levels, while the lowest variation ( 7%) wasrecorded in experiment contained the minimum amount of yeast extract followed by one with thelowest concentration ofmeat extract ( 9%). The only medium resulted in lower a-amylase activity (27%)at pH = 4.5 was the treatment with meat and yeast extracts at ( 1) levels. The effect of C/N ratio on theresponses was also investigated.
Bahman Ebrahimi,Seyed Abbas Shojaosadati,Seyyed Mohammad Mousavi 한국생물공학회 2010 Biotechnology and Bioprocess Engineering Vol.15 No.3
A flow-through system based on acetylcholineesterase (AChE) was studied. The system was prepared by mixing AChE and a multiwall carbon nanotube (MWCNT). Two important parameters, the ratios of AChE:MWCNT (X1) and AChE-MWCNT:sol-gel (X2)were optimized using response surface methodology. The results revealed that an enzyme immobilized within the MWCNT-sol-gel was more effective compared to one conducted with sol-gel. The optimum feed flow rate was 0.4 mL/min and ATChI concentration was found to be 1 mM. The optimum ratios of X1 and X2 for immobilization on ceramic packing were 1.07 and 0.43, respectively. The sensitivity of this flow-through system was 1.82 × 10−5/μM and long-term stability analyzed after 120 days was 74% of initial absorbance. With respect to an incubation time of 14 min, the detection limit for paraoxon was 7.3 × 10−12 mol.
Porosity changes during packed bed solid-state fermentation
A. Karimi,S.A. Shojaosadati,P. Hejazi,E. Vasheghani-Farahani,M. Hashemi 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.6
Porosity is one of the key parameters in a typical solid-state fermentationmodel that represents the intraand inter-particle void spaces in bioreactor bed. In this research, the particle density and moisturecontent of the bed were used to determine the porosity changes of the solid substrate bed during thefermentation of Aspergillus niger in a laboratory scale packed-bed bioreactor. Biomass growth was alsomonitored by means of measuring CO2 and O2 in the bioreactors outlet gas online. As such, anexperimental relationship between humidity and porosity has been developed and can be used in solidstatefermentation modeling.
Safavi, M.S.,Shojaosadati, S.A.,Yang, H.G.,Kim, Y.,Park, E.J.,Lee, K.C.,Na, D.H. Elsevier/North Holland 2017 International Journal of Pharmaceutics Vol. No.
The purpose of this study was to prepare curcumin-loaded bovine serum albumin nanoparticles (CCM-BSA-NPs) by reducing agent-free self-assembly at room temperature. A 2<SUP>4</SUP> factorial design approach was used to investigate the CCM-BSA-NP preparation process at different pH values, temperatures, dithiothreitol amounts, and CCM/BSA mass ratios. Increasing the ionic strength enabled preparation of CCM-BSA-NPs at 25<SUP>o</SUP>C without reducing agent. CCM-BSA-NPs prepared under the optimized conditions at 25<SUP>o</SUP>C showed a particle size of 110+/-6nm, yield of 88.5%, and drug loading of 7.1%. The CCM-BSA-NPs showed strong antioxidant activity and neuroprotective effects in glutamate-induced mouse hippocampal neuronal HT22 cells. This study suggests that ionic strength can be a key parameter affecting the preparation of albumin-based NPs.
Niloofar Nasirpour,Seyyed Mohammad Mousavi,Seyed Abbas Shojaosadati 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.5
We investigated a novel wastewater treatment method for the remediation of crude oil refinery effluents with large number of recalcitrant organic compounds. The treatment system consists of an up-flow anaerobic sludge blanket (UASB) reactor and an aerobic packed-bed biofilm reactor (PBBR) in combinatory pattern to increase the efficiency of treatment and to remove polycyclic aromatic hydrocarbons (PAHs) of the wastewater. The mean chemical oxygen demand (COD) removal efficiency in the UASB reactor and PBBR over 118 days of sampling was 68.48% and 38.28%, respectively. The total COD removal efficiency of the system was 81.07%. The GC-MS abundance and area values for each of the substances in the effluent decreased greatly from the corresponding value in the influent. Specifically, the PAHs were totally removed during the treatment process. This study presents a feasible technology for the treatment of refinery effluents.
Amir Maghsoudi,Safoura Hosseini,Seyed Abbas Shojaosadati,Ebrahim Vasheghani-Farahani,Mohsen Nosrati,Ali Bahrami 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.1
Developing novel methanol-feeding strategies for the improved production of heterologous proteins in high cell-density fed-batch cultures of Pichia pastoris has been of great interest during recent years. In this study, a recombinant P. pastoris strain (GS115/His+ Mut+) producing β-galactosidase (β-Gal) was used to investigate conventional feeding strategies and to develop a new strategy to increase the recombinant protein production during fedbatch cultures on methanol. Three types of conventional methanol-feeding strategies, including μ-stat, dissolved oxygen-stat (DO-stat) and constant methanol concentration were investigated and compared with respect to alcohol oxidase (AOX), formate dehydrogenase (FDH) and β-gal activities, and cell dry weight (CDW), methanol, and formaldehyde concentration variations during the production phase. Methanol feeding with μ-stat 0.025/h exhibited the highest β-gal activity. Supplementing ammonium and magnesium in μ-stat 0.025/h did not affect the cell growth or methanol or formaldehyde concentrations throughout the fermentation but did improved the maximum β-gal activity from 148.2 to 158.1 kU/mL. A new three-step methanol-feeding strategy was developed based on the results obtained from conventional feeding strategies, which started with μ-stat 0.025/ h for 5 h, then μ-stat 0.030/h, and finally, was switched to DO-stat when maintaining the DO above 20% air saturation became difficult. Implementation of this new feeding strategy resulted in a CDW of 107.2 ±0.7 g/L, AOX specific activity of 0.1890 ± 0.0030 U/mg CDW, and β-gal activity of 173.5 ± 2.1 kU/mL after 29 h of fermentation, which shows a 5.6, 29.1, and 15.7%increase in CDW, AOX, and β-gal activity, respectively,compared to that of μ-stat at 0.025/h.
The possible mechanisms involved in nanoparticles biosynthesis
H. Moteshafi,S.M. Mousavi,S.A. Shojaosadati 한국공업화학회 2012 Journal of Industrial and Engineering Chemistry Vol.18 No.6
Several aspects of silver nanoparticle (NP) biosynthesis using Fusarium oxysporum under different conditions, that have not been previously considered, were explored. NP synthesis with different nitrate concentrations and silver sulfate instead of silver nitrate was investigated. The ability of F. oxysporum to synthesize NP under oxygen-limited conditions was also evaluated. In the presence of a nitrate reductase inhibitor, different mechanism rather than the reduction of metal ions by a nitrate-dependent reductase,was revealed. SDS-PAGE analysis indicated that a protein with 14 kDa molecular weight could be involved in a specific response of the fungus in the presence of silver ions.
S.O. Rastegar,S.M. Mousavi,M. Rezaei,S.A. Shojaosadati 한국공업화학회 2014 Journal of Industrial and Engineering Chemistry Vol.20 No.5
This study examines the bioleaching ability of a molybdenite concentrate by an adapted bacterium in batch cultures. Acidianus brierleyi was used to mobilize Mo/Cu/Fe from the molybdenite concentrate. Response surface methodology (RSM) was applied to predict the behavior of effective parameters and their interactions in the bioleaching process. Obtained results showed that the best model for the recovery of each metal was the reduced cubic model. At the optimum condition, (0.3%, (w/v) pulp density, 0.2%, (w/v) amount of sulfur, and 1% (v/v) inoculation percentage), the maximum values of recovery were achieved at 2%, 100%, and 100% for Mo, Cu, and Fe, respectively, with a pH of 2.0, and a temperature of 60 ℃.