C형 간염바이러스항체 검출을 위한3세대 효소면역측정 시약의 비교

이장혁,서순팔,기승정,송정원,신명근,신종희,양동욱,김세종 대한임상병리학회 1997 대한임상병리학회지 Vol.17 No.4

高麗史 叛逆 列傳 譯註(1)

申東根 영남이공대학 1997 論文集 Vol.26 No.-

This study is to interpreter and comment on Kim Chi Yang(金政陽) and Kang Jo(康兆) Yeoljun(列傳) in Koryosa(高麗史) Revolt Yeoljun.

《高麗史》 叛逆列傳 譯註 Ⅱ

申東根 영남이공대학 1999 論文集 Vol.28 No.-

This Paper is to interpretation the parts of the contents in Koryosa(高麗史) Revolt Yeoljun(判逆列傳), of Hwan, sin-gil(桓宣吉) Im, choon-gilt(林春吉) and Yi, hun-am(伊昕嚴) who tried to betray the King, Wang gun(王建) shortly after the foundation of Koryo.

Visible Human Data의 3차원 가시화에 대한 연구

최형근,김동현,탁계래,신현준 건국대학교 의과학연구소 2000 건국의과학학술지 Vol.10 No.-

Visualization is the process of exploring, transforming and viewing data as images to gain understanding and insight into the data. Visualization in medicine is helpful in understanding human anatomy by presenting the information in a form that is not only pleasing but also easily recognizable as well as in gaining functional attributes of medical systems, such as biomechanical and physiological properties. Medical applications include accurate anatomy and function mapping, enhanced diagnosis. accurate treatment planning and rehearsal, and education/training. The Visible Human data set of the National Library of Medicine, the most complete anatomically detailed. computerized database of the human body ever assembled, has been used for these purposes. In this study, we have visualized the Visible Human Data set with VTK(Visualization ToolKit, Kitware Inc.), IAP(Image Application Platform, ISG Inc.) and Visual C++ 6.0. A motivation of this study is the fact that computer imaging techniques have become an important diagnostic tool in the practice of modern medicine. The 3D visualization system consists of a basic 2D image processing such as filtering, panning, zooming and measuring image as well as 3D image processing such as multiplanar reformatting, surface rendering and volume rendering. The wide-spread use and accessibility of the web have been required to provide the visualization of 3D image on the web. Thus, we have developed web-based medical 3D visualization system that supports World-Wide-Web using VRML and client/server architecture.

경부도상 피판을 이용한 구강내 결손부의 재건

양동규,신상훈,서종천,이성근,김종렬 大韓顎顔面成形再建外科學會 1995 Maxillofacial Plastic Reconstructive Surgery Vol.17 No.3

Various cutaneous as well as myocutaneous flaps have been designed for the reconstruction of tissue defects caused by the excision of oral cancer. Among these flaps, cervical island skin flap have been introduced by Farr et al and more have developed by Tashiro et al. This flap has many advantages. The flap minimizes donor size by use of cervical operation wound, flap size available is adequate for most oral defects and the procedure is relatively simple and time saving. However, this flap is not applicable in patients where there are large tissue defects and metastasis is suspected. We used this flap for it's rapid simple, and effective, primary closure of oral defects after cancer ablation and we have found this flap very useful for the reconstruction of relatively small oral defects.

IMT-2000/WLAN/DMB 단말기용 광대역 PIFA 설계 및 SAR 분석

최동근,신찬수,김남 충북대학교 컴퓨터정보통신 연구소 2004 컴퓨터정보통신연구 Vol.12 No.2

본 논문에서는 IMT-2000/WLAN/DMB 단말기용 광대역 PIFA(Planar Inverted-F Antenna)를 설계하였다. 일반적인 PIFA 구조의 단점인 협대역 특성을 개선하기 위하여 미앤더 구조를 변형한 두 개의 branch 형태를 사용하였다. 안테나의 크기를 효율적으로 줄이기 위하여 단락스트립을 사용하였고, 미앤더 형태에 따른 방사패치를 가지는 PIFA를 제안하였다. 설계 제작된 안테나의 -10dB 반사 손실 대역폭은 38.2 %(1.84~2.71 GHz)로 IMT-2000/WLAN/DMB 대역을 모두 만족하는 광대역 특성을 얻었다. 전자파 흡수율의 감소량을 알아보기 위해 실제로 폴더형 구조를 가지는 휴대폰에 제안된 안테나를 탑재하여 1 g 및 10 g 평균 첨두 SAR를 측정한 값과 시뮬레이션 결과를 비교하였다. 그 결과는 아주 유사하게 나왔으며, 1 g 및 10 g 평균 첨두 SAR 제한치인 1.6 W/kg, 2 W/kg보다 낮은 값을 얻었다. This paper proposed a new broadband PIFA(Planar Inverted-F Antenna) for IMT-2000/WLAN/DMB terminal. Two branches type changed meander structure was utilized in order to improve the characteristics of PIFA which usually has a narrow band. The shorting strip between the ground plane and the antenna and meander-type radiation patches were used in order to minimize the size of the antenna. The -10 dB return loss bandwidth of a realized antenna was 38.2 %(1.84~2.71 GHz), which contains the broadband with triple band. And the simulated and measured values of 1 g and 10 g averaged peak SAR on human head caused by the triple band PIFA mounted on folder-type handsets were analyzed and discussed. As a result, the measured 1 g and 10 g averaged peak SARs of PIFA was similar to simulated values and were lower than the 1.6 W/kg, 2 W/kg of 1 g and 10 g averaged peak SAR limits.

苦汁으로 부터 炭酸마그네슘과 酸化마그네슘의 最適合成條件에 關한 硏究

鄭東燻,辛宗根,申和雨 圓光大學校 1989 論文集 Vol.23 No.2

고즙과 탄산나트륨을 원료로 1)반응액의 온도, 2)반응액의 농도, 3)혼합비율(〔Bittern〕/〔Na₂CO₃〕), 4)작용시간 및 5)건조온도를 합성요인으로 하여 Box-Wilson 실험계획법에 의해 탄산마그네슘의 최적합성조건을 추구하고 그 중 우수제품에 대해 D.S.C.를 측정하여 이를 기준으로 하소시켜 산화마그네슘을 제조하여 용적시험, 제산도시험 및 광학현미경사진을 관찰한 결과 1. 탄산마그네슘의 최적합성조건의 범위는 ①반응액의 온도범위는 50∼70℃ ②반응액의 농도범위는 고즙은 37.3∼38.1%, 탄산나트륨은 9.9∼10.3%, ③혼합비율(〔Bittern〕/〔Na₂CO₃〕)의 범위는 1.485∼1.503, ④작용시간의 범위는 10∼12분, ⑤건조온도의 범위는 73∼83℃이다. 2. 시료탄산마그네슘의 D.S.C.측정결과 75℃정도에서 결정수의 이탈로 보이는 흡열Peak를 나타냈고, 290℃정도에서 구조수의 이탈로 생각되는 흡열Peak를 나타냈으며, 408℃정도에서 탄산깨스의 이탈로 생각되는 흡열Peak를 나타냈다. 3. 탄산마그네슘의 하소온도는 500∼600℃가 적당하겠으며, 용적시험결과는 모두 경질품이었고, 시판품보다 모두 용적시험치가 큰 결과를 나타냈다. 4. 제산도시험의 결과는 시판품의 제산능보다 모두 크며 광학현미경사진에 의한 관찰을 하였다. Optimal Synthesis Condition of Magnesium Carbonate were investigated from the viewpoint of bulkiness according to a randomized complete block design proposed by G.E.P. Box and K.B. Wilson. Bittern and Sodium Carbonate were utilized as reactants in order to prepare Magnesium Carbonate. It was found that optimum temperature range of reactant solutions was 50∼70℃ and the optimum concentration range of the reactant solutions was 37.3∼38.1%(Bittern), 9.9∼10.3%(Sodium Carbonate), on the viewpoint of bulkiness. The optimum mole ratio of Bittern to Sodium Carbonate was in the range of 1.485∼1.503and the optimum reacting time range was 10∼12minutes. The optimum drying temperature range was 73∼83℃. Magnesium Oxide comes two types of granules when prepared by calcination of Magnesium Carbonate. The outcome of D.S.C. indicated a desolvation of Magnesium Carbonate occurred at about 75℃. The dehydration of the compound ceased at about 290℃ and the decarboxylation ceased at about 408℃. The physical and chemical properties of Magnesium Oxide as medicine were studied by use of Volume Test, Optical microscopic photographs and Acid Consuming Capacity measurments.

중소기업형 CIM시스템 구축을 위한 생산계획수립 및 실적파악 모듈

최후곤,신완선,장중순,서준성,여명구,안동근,김진봉 成均館大學校 科學技術硏究所 1996 論文集 Vol.47 No.2

This study Focuses on developing several modules involved in an integrated production information system for medium- or small-sized industries. The modules of order management, quality control, production scheduling, and shop management are executed for an example case industry. The potential values of this research includes that the major information modules to collect, analysis, and display production data and shop data for constructing the computer integrated manufacturing system(CIMS) are programmed with various features of medium- or small-sized industries

내열성 Chitinase 생산균주의 분리 및 효소생산 특성

홍범식,윤호근,신동훈,조홍연 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

자연계 고온환경으로부터 내열성 chitinase 우수하고 반응산물로 N-acetyl-D-glucosamine 이량체(GlcNAc)_2를 생산하는 균주를 분리 선별하고 Bacillus licheniformis KFB-C14로 동정하였다. 선별균주의 효소생산 특성은 탄소원으로서 효소기질인 colloidal chitin이 첨가될 때만이 생합성이 유도되었으며 유도제의 첨가시기에 의해 효소생산이 크게 영향을 받았다. 각종 무기, 유기태 질소원 중 yeast extract가 활성과 비활성을 각각 약 2배 증가시켰으며 높은 친화도를 나타내었다. 균의 최대생육과 효소의 최대생산온도는 55℃이었다. 본 균주의 내열성 chitinase 생산에 미치는 최적배양조건은 1.2% colloidal chitin, 0.15% K_2HPO_4, 0.05% KH_2PO_4 0.01% MgSO_4·7H_2O, 0.1% yeast extract, pH 6.5의 배지를 55℃, 150rpm에서 40시간 회전진탕배양 하였을 때로 3.89 units/ml의 효소활성과 7.4 units/mg의 비활성을 나타내었다. A strain capable of producing thermostable chitinase suitable for chitooligosaccharide production was isolated from high temperature environment and identified as Bacillus licheniformis. The chitinase from Bacillus licheniformis KFB-C14 was only induced by addition of colloidal chitin into the basal medium as carbon source, showing the decrease of the chitinase production by supplemental addition of other carbon sources into the medium containing 1.0% colloidal chitin. Among organic and inorganic nitrogen sources, yeast extract was the most effective for the increase of total activity and specific activity, and had high affinity for the enzyme production. The optimum temperature of cell growth and thermostable chitinase production was 55℃. The optimum culture medium was composed of 1.2% colloidal chitin, 0.15% K_2HPO_4, 0.05% KH_2PO_4 0.01% MgSO_4·7H_2O, 0.1% yeast extract (pH 6.5). Bacillus licheniformis KFB-C14 produced the thermostable chitinase of 3.89 units per culture fluid and 7.4 units per mg protein under rotary shaking at 150 rpm for 40 hr.

Bacillus licheniformis KFB-C14가 생산하는 내열성 Chitinase의 정체 및 특성

홍범식,윤호근,신동훈,조홍연 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.5

Bacillus licheniformis KFB-C14가 생산하는 내열성 chitinase를 30~70% ammonium sulfate fractionation, DEAE-Toyopearl 650M, Butyl-Toyopearl 650M, TSK-Gel Toyopearl HW-55F에 의해 정제도 66배, 수율 21%로 전기영동적으로 균일하게 정제하였다. 정제 단백질은 gel permeation chromatography에 의해 86,000±2,000의 분자량을 나타내었으며, SDS 전기영동에 의해 밝혀진 본 효소의 subunit 구조는 monomer였다. 효소 단백질의 안정성을 검토한 결과 80℃에서 30분 열처리에 의해 56%, 37℃에서 20분간 40% ethanol과 ethyl acetate, 단백질 변성제 등의 처리시에도 50% 이상의 잔존활성을 나타냄으로써 공업적으로 유용성이 높은 안정한 단백질로 판명되었다. 효소반응의 최적 pH와 온도는 pH 6.0과 60℃이었고 Mn^2+ 이온에 의해 효소 활성이 저해되었으나 EDTA, N-ethylmaleimide, p-chloromercuribenzoate 등에 의한 활성감소는 관찰되지 않음으로써 금속효소 또는 thiol계 효소에 속하지 않음을 알 수 있었다. 본 효소는 colloidal chitin, 시판용 chitin에는 반응성이 높았으나 exo형 chitinase의 대표적인 기질인 p-nitrophenyl-2-aectamido-2deoxy-β-glucopyranoside, NN'-diacetylchitobiose에는 전혀 반응성을 보이지 않는 전형적인 endo형의 chitinase였다. 본 효소는 colloidal chitin으로부터 주로 (GlcNAc)_2를, 반응시간 경과에 따라 (GlcNAc)_1과 (GlcNAc)_3을 생성하는 반응성을 보였다. Chitinase (EC 3.2.1.14) from culture fluid of Bacillus licheniformis KFB-C14 was purified 66-folds to homogenity in overall yield of 21% by ammonium sulfate fractionation, DEAE-Toyopearl, Butyl-Toyopearl and TSK-Gel HW-55F column chromatography. The enzyme protein had a molecular weight of about 86,000 and was composed of one subunit. The enzyme was significantly stable not only at high temperature but also on treatment with organic solvents and protein denaturants such as SDS, urea and guanidine·HCl. The optimum temperature and pH for reaction was 60℃ and 6.0,respectively. The enzyme activity was inhibited by only Mn^2+ ion, but not inhibited by EDTA, N-ethylmaleimide and pCMB. The enzyme had high activity with colloidal chitin (V_max: 421) and commercial chitin (V_max :480), but not with typical substrates of exo type chitinase. The thermostable chitinase had an useful reactivity for producing functional chitooligosaccharide, showing the production of (GlcNAc)_1, (GlcNAc)_3, and (GlcNAc)_2 as major product.