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Shin, Eun Kyoung,Lee, Sa Ra,Kim, Mi Kyoung,Kang, Eun Ji,Ju, Woong,Lee, Shi Nae,Han, Woon Sup,Kim, Seung Cheol Wiley Subscription Services, Inc., A Wiley Company 2008 Diagnostic cytopathology Vol.36 No.5
<P>For cervical cancer screening, HPV-DNA test is expensive and is not easily available in all clinical situations. Thus, we investigated the role of p16<SUP>ink4a</SUP> immunostaining as another adjunct test to diagnose cervical neoplasia in equivocal liquid based cytology. Eighty-seven patients were randomly selected for this study (3 patients with normal, 84 patients with abnormal including 24 ASCUS, 30 LSIL, and 30 HSIL). We performed p16<SUP>ink4a</SUP> immunostaining on ThinPrep® slide and on each case from the corresponding cervical biopsy tissues. High-risk HPV-DNA testing was also performed on all the subjects. We found that the immunoreactivity of p16<SUP>ink4a</SUP> is strongly correlated with the grade of cytologic and histologic diagnoses as well as with Hybrid Capture 2. In comparing the p16<SUP>ink4a</SUP> immunostaining with the Hybrid Capture 2 for accuracy of the diagnosis of CIN II/III or a higher-grade disease in the case of ASCUS/LSIL on ThinPrep®, no significant differences were observed. Our data implies that p16<SUP>ink4a</SUP> immunocytochemical staining in liquid-based cytology specimens might be used as a good adjunct test to predict cervical histology in equivocal ThinPrep® tests. Diagn. Cytopathol. 2008;36:311–316. © 2008 Wiley-Liss, Inc.</P>
Ha, Hye-Yeong,Cho, Ik-Hyun,Lee, Kang-Woo,Lee, Ko-Woon,Song, Ji-Young,Kim, Kyoung-Shim,Yu, Young-Mi,Lee, Ja-Kyeong,Song, Jin-Sook,Yang, Sung-Don,Shin, Hee-Sup,Han, Pyung-Lim Elsevier 2005 Developmental Biology Vol.277 No.1
<P><B>Abstract</B></P><P>The JNK interacting protein, JSAP1, has been identified as a scaffold protein for mitogen-activated protein kinase (MAPK) signaling pathways and as a linker protein for the cargo transport along the axons. To investigate the physiological function of JSAP1 in vivo, we generated mice lacking JSAP1. The JSAP1 null mutation produced various developmental deficits in the brain, including an axon guidance defect of the corpus callosum, in which phospho-FAK and phospho-JNK were distributed at reduced levels. The axon guidance defect of the corpus callosum in the <I>jsap1</I><SUP><I>−/−</I></SUP> brain was correlated with the misplacement of glial sling cells, which reverted to their normal position after the transgenic expression of JNK interacting protein 1(JIP1). The transgenic JIP1 partially rescued the axon guidance defect of the corpus callosum and the anterior commissure of the <I>jsap1</I><SUP><I>−/−</I></SUP> brain. The JSAP1 null mutation impaired the normal distribution of the Ca<SUP>+2</SUP> regulating protein, calretinin, but not the synaptic vesicle marker, SNAP-25, along the axons of the thalamocortical tract. These results suggest that JSAP1 is required for the axon guidance of the telencephalic commissures and the distribution of cellular protein(s) along axons in vivo, and that the signaling network organized commonly by JIP1 and JSAP1 regulates the axon guidance in the developing brain.</P>
Shin Jeong-Sup,Choi Yong-Woon,Sung Hark-Mo,Ryu Yeon-Woo,Kim In-Seop The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.1
With particular regards to the hepatitis A virus (HAV), a terminal dry-heat treatment ($100^{\circ}C$ for 30 min) process, following lyophilization, was developed to improve the virus safety of a solvent/detergent-treated antihemophilic factor IX concentrate. The loss of factor IX activity during dry-heat treatment was of about 3%, as estimated by a clotting assay. No substantial changes were observed in the physical and biochemical characteristics of the dry-heat-treated factor IX compared with those of the factor IX before dry-heat treatment. The dry-heat-treated factor IX was stable for up to 24 months at $4^{\circ}C$, The dry-heat treatment after lyophilization was an effective process for inactivating viruses. The HAV and murine encephalomyocarditis virus (EMCV) were completely inactivated to below detectable levels within 10 min of the dry-heat treatment. Porcine parvovirus (PPV) and bovine herpes virus (BHV) were potentially sensitive to the treatment. The log reduction factors achieved during lyophilization and dry-heat treatment were ${\ge}5.60$ for HAV, ${\ge}6.08$ for EMCV, 2.64 for PPV, and 3.59 for BHV. These results indicate that dry-heat treatment improves the virus safety of factor IX concentrates, without destroying the activity. Moreover, the treatment represents an effective measure for the inactivation of non-lipid enveloped viruses, in particular HAV, which is resistant to solvent/detergent treatment.
이신원(Shin-Won Lee),최연철(Yeon-Chul Choi),이운성(Woon-Sung Lee),안현식(Hyun-Sik Ahn),김상섭(Sang-Sup Kim) 한국자동차공학회 1995 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1995 No.11_1
A systematic design environment for integrated control of chassis system is developed and its effectiveness is demonstrated in this paper. 4WSS based on yaw rate feedback and a hydropneumatic ASS based on PID control are developed first. A decision logic to identify present driving conditions is also developed using sensed vehicle signals such as vertical acceleration. steering angle, lateral acceleration, etc. An algorithm that adjusts control parameters of the 4WSS and the ASS automatically according to driving conditions is proposed. MATRIXx/SYSTEMBULLD software is used to illustrate that vehicle stability, maneuverability, and ride comfort are improved by the proposed integrated control method.<br/>
( Yong Sup Shin ),( Hyung Won Kim ),( Chang Deok Kim ),( Hyun Woo Kim ),( Jin Woon Park ),( Sunggyun Jung ),( Jeung Hoon Lee ),( Young Kwon Ko ),( Young Ho Lee ) 대한피부과학회 2015 Annals of Dermatology Vol.27 No.4
Background: Protease-activated receptor 2 (PAR-2) participates in various biological activities, including the regulation of epidermal barrier homeostasis, inflammation, pain perception, and melanosome transfer in the skin. Objective: To evaluate the basic physiological role of PAR-2 in skin. Methods: We investigated PAR-2 expression in human epidermis, skin tumors, and cultured epidermal cells using western blot and immunohistochemical analysis. Additionally, we examined the effect of the PAR-2 agonist, SLIGRL-NH2, on cultured keratinocytes. Results: Strong PAR-2 immunoreactivity was observed in the granular layer of normal human skin and the acrosyringium of the eccrine sweat glands. In contrast, weak PAR-2 immunoreactivity was seen in the granular layer of callused skin and in the duct and gland cells of the eccrine sweat glands. Interestingly, PAR-2 immunoreactivity was very weak or absent in the tumor cells of squamous cell carcinoma (SCC) and syringoma. PAR-2 was detected in primary keratinocytes and SV-40T-transformed human epidermal keratinocytes (SV-HEKs), an immortalized keratinocyte cell line, but not in SCC12 cells. SV-HEKs that were fully differentiated following calcium treatment displayed higher PAR-2 expression than undifferentiated SV-HEKs. Treatment of cultured SV-HEKs with PAR-2 agonist increased loricrin and filaggrin expression, a terminal differentiation marker. Conclusion: Our data suggest that PAR-2 is associated with terminal differentiation of epidermis and eccrine sweat glands. (Ann Dermatol 27(4) 364∼370, 2015)