Heme oxygenase-1 induced by desoxo-narchinol-A attenuated the severity of acute pancreatitis via blockade of neutrophil infiltration

Bae, Gi-Sang,Kim, Dong-Goo,Jo, Il-Joo,Choi, Sun-Bok,Kim, Myoung-Jin,Shin, Joon Yeon,Kim, Dong-Uk,Song, Ho-Joon,Joo, Myungsoo,Park, Sung-Joo ELSEVIER 2019 INTERNATIONAL IMMUNOPHARMACOLOGY Vol.69 No.-

<P><B>Abstract</B></P> <P>Heme oxygenase-1 (HO-1) has an anti-inflammatory action in acute pancreatitis (AP). However, its mechanism of action and natural compounds/drugs to induce HO-1 in pancreas are not well understood. In this study, we investigated the regulatory mechanisms of HO-1 during AP using desoxo-narchinol-A (DN), the natural compound inducing HO-1 in the pancreas. Female C57/BL6 Mice were intraperitoneally injected with supramaximal concentrations of cerulein (50 μg/kg) hourly for 6 h to induce AP. DMSO or DN was administered intraperitoneally, then mice were sacrificed 6 h after the final cerulein injection. Administration of DN increased pancreatic HO-1 expression through activation of activating protein-1, mediated by mitogen-activated protein kinases. Furthermore, DN treatment reduced the pancreatic weight-to-body weight ratio as well as production of digestive enzymes and pro-inflammatory cytokines. Inhibition of HO-1 by tin protoporphyrin IX abolished the protective effects of DN on pancreatic damage. Additionally, DN treatment inhibited neutrophil infiltration into the pancreas via regulation of chemokine (C-X-C motif) ligand 2 (CXCL2) by HO-1. Our results suggest that DN is an effective inducer of HO-1 in the pancreas, and that HO-1 regulates neutrophil infiltration in AP via CXCL2 inhibition.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Desoxo-narchinol-A (DN) is a natural compound of HO-1 inducer in pancreas. </LI> <LI> Mechanism of DN-induced HO-1 is mediated by MAPK/Activator Protein-1/HO-1 signaling. </LI> <LI> DN-induced HO-1 blocks neutrophil infiltration into pancreas via inhibition of CXCL2. </LI> <LI> DN inhibits cerulein-induced acute pancreatitis (AP) and AP-associated lung injury. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Induction of heme oxygenase-1 protects against podocyte apoptosis under diabetic conditions

Lee, Sang Choel,Han, Seung Hyeok,Li, Jin Ji,Lee, Sun Ha,Jung, Dong-Sub,Kwak, Seung-Jae,Kim, Seung Hye,Kim, Dong Ki,Yoo, Tae-Hyun,Kim, Jin Hyun,Chang, Se-Ho,Han, Dae Suk,Kang, Shin-Wook International Society of Nephrology 2009 Kidney international Vol.76 No.8

Heme oxygenase-1 (HO-1) is an anti-oxidant enzyme normally upregulated in response to oxidant injury. Here we determined the role of HO-1 in podocyte apoptosis in glomeruli of streptozotocin-treated rats and in immortalized mouse podocytes cultured in media containing normal or high glucose. HO-1 expression, its activity, the ratio of Bax/Bcl-2 protein, and active caspase-3 fragments were all significantly higher in isolated glomeruli of diabetic rats and in high glucose–treated podocytes. These increases were inhibited by zinc protoporphyrin treatment of the rats or by HO-1 siRNA treatment of the podocytes in culture. The number of apoptotic cells was also significantly increased in the glomeruli of diabetic rats and in high glucose–treated podocytes. Inhibition of HO-1 accentuated the increase in apoptotic cells both in vivo and in vitro. Our findings suggest that HO-1 expression protects against podocyte apoptosis under diabetic conditions.

동의보감 탕액편에 기재된 식이본초의 독성유무에 대한 분류 연구

신호동(Ho-Dong Shin),정종운(Jong-Un Jeong) 대한한의학회 2011 대한한의학회지 Vol.32 No.1

혈관질환 정보관리 시스템

김동익,김덕경,허세호,이병붕,김용신,김은숙,문지영,도영수,신성욱,김동수,김만태,진재욱,김용신 대한혈관외과학회 2002 Vascular Specialist International Vol.18 No.1

As medical technology progresses rapidly, there is a rise in the average age along with the Korean dietary lifestyle becoming more westernized, which leads to an increase in the number of vascular disease patients in Korea. Thus, we need to manage the medical information of a disease systematically in order to diagnose and treat constructively. However, since there has been no standardized method of man agement to date, a great deal of information could not be properly utilized nor studied. Therefore, the departments of Cardiology, Radiology and Neurology of Samsung Seoul Hospital recently got together to develop an information management system called the Vascular Data System. This program was developed to be run on win98 O/S, upper Pentium Ⅲ, and upper 128 MB Memory, and its source code is Dephi 4.0. It was configured for the user to set the configurations as well as do a variety of search and analysis. If this program were to be updated continuously, it may be used extensively as well as in various parts of clinical research activities.

만성 견비통에 대한 동씨침 치료의 무작위 대조군의 임상 연구

김찬영,권나현,신예지,남동우,김건형,김종인,최도영,이윤호,이재동 WHO COLLABORATING CENTRE FOR TRADITIONAL MEDICINE 2007 東西醫學硏究所 論文集 Vol.2007 No.-

Objectives : To observe the effect of acupuncture treatment in chronic shoulder pain patients. Methods : 36 voluntary patients were randomly assigned to an acupuncture treatment group(E GrouP, n=18) and a control group(C GrouP, n=18). The E Group patients received acupuncture treatment on LI_(15), TE_(14), GB_(21) and Master Dong's acupuncture points, Shin-gwan and Gyun-joong, twice a week for four weeks. The C Group patients received no treatment. All patients in both groups were instructed to practise self exercise in their daily lives. Evaluations were made at baseline and after four weeks of study. The Constant Shoulder Assessment(CSA), Shoulder Pain and Disability Index(SPADI) and the patient's subjective pain was measured by Visual Analogue Scale(VAS). The obtained data was analyzed. Results '. The E Group showed significant(p<0.05) improvement in CSA, SPADI and VAS after four weeks of treatment. The C Group showed significant(p<0.05) improvement in CSA, but the change of SPADI and VAS was insignificant(P>0.05). CSA and SPADI of E Group significantly(p<0.05) improved compared to the C GrouP, but the difference of VAS change in the two groups was insignificant(p>0.05). Conclusions : Four weeks of acupuncture treatment significantly improved CSA, SPADI and VAS. The improvement of CSA and SPADI was significant(p<0.05) compared to untreated patients.

구성주의 교육철학과 교수-학습

신동로,주호수 全北大學校 敎育大學院 1998 敎育論叢 Vol.18 No.-

The study reviews the current changes in the Korean education. It identifies constructivism as the change agent for the classrooms. Students learn more when the practices are characterized as child-centered, experiential, social, constructivist, and challenging learning environment. Thus, the recent educational reform have been focused on setting up such learning environment that enhance student's learning in the classroom. These changes can be made only by the classroom teachers' paradigm-shift in teaching-learning practices form objectivist to constructivist. The study reviews constructivism's philosophy in the teaching-leaning practices. The constructivist advocates that the learning is a process of knowledge construction. In constructivistic learning envionment, larning is also knowledge-dependent, and highly tuned to the situation in which it takes place. Thus, the constructivistic learning should be occurred in learner-centered, knowledge constructed learning environment. For better education, there should be as many teaching-learning methods as needed for each learning settings. If educators not changed their philosophical orientation in education from objectivism to constructivism, there will be no actual changes in the classrooms. There should be a paradigm-shift in teaching and learning from objectivism to constructivism. Therefore, this paper attempted to introduce the Constructivist Learning Model(CLM) for Improving Science Education.

莊子 「齊物論 」硏究

申東浩 충남대학교 인문과학연구소 1980 인문학연구 Vol.7 No.2

The aim of this paper is to survey the meaning of "Chi Wu Lun", the subject matter of Chapter II in Chuang-tzu. According to the traditional concept, some scholars assert Chi Wu Lun means "all things are the same afterer all", and the others assert that it means "all the opposed concepts become the same after all." The author has got the following conclusion. Many different concepts are derived from the fact that all things are the different other things. If we find all things are the same after all, we are able to perceive that different other concepts become the same in the long run. In this viewpoint, it is known that the two concepts are connected each other in Chi Wu Lun Chuang Tzu suggested.

全北의 交通事故 特性 및 事故減少對策에 관한 硏究

申動鎬 圓光大學校 附設 都市地域開發硏究所 1994 都市計劃論文集 Vol.1 No.-

Traffic accidents bring about not only personnel injuries but also enormous social economic losses. In Chunbuk Province, the total amount of traffic accidents has increased at the annual average rate of 8.8% during recent 10 years (1982∼1992), which is higher than that of 6.2% in the whole country of Korea during the same period. The purposes of this study were to investigate the characteristics of traffic accidents and to propose the countermeasures diminishing traffic accidents, paticulary appropriate to the regional dimension, in Chunbuk Province. In Chunbuk Province the proportion of pedestrian accidents and the rate of traffic deaths to the whole traffic accidents were respectively higher than those of the whole country paticularly on national highways. In this study the countermeasures to diminish these traffic accidents were proposed from the viewpoint of comprehensive approach including human, road environmental and institutional aspects.

희생양극을 이용한 자동차 소음기의 음극방식에 관한 연구

신동호,심재주,최윤석,김정구 대한금속재료학회 2004 대한금속·재료학회지 Vol.42 No.12

In order to apply a sacrificial anode cathodic protection system for automotive muffler materials, determination of reliable sacrificial anode and its protection ability were evaluated by laboratory tests in synthetic condensed water. Three kinds of sacrificial anodes were selected: aluminum (AI), zinc (Zn) and magnesium (Mg), and the muffler materials were Al-coated steel and uncoated steel. The corrosion behavior in synthetic condensed water for the muffler materials and sacrificial anodes was investigated by potentiodynamic tests. The cathodic protection ability was evaluated by galvanic corrosion test and weight-loss test. The results of the potentiodynamic test indicated that Al was less suitable for sacrificial anode because it became passive. Mg was also unqualified due to the high dissolution rate. However, Zn was adequate for sacrificial anode because of its low dissolution rate, high stability and suitable potential difference. Results of weight-loss test demonstrated that the corrosion rate of Al-coated steel was reduced from 0.028 to 0.018 mm/year, and that of uncoated steel was 0.08 to 0.002 mm/year by coupling with Zn anode. In addition, the proper area ratio of anode to cathode was 1 : 10. From these results, it can be concluded that Zn anode can protect both the Al-coating with passivation, and steel substrate with low dissolution rate. (Received January 8, 2004)

하수 방류수 살균소독을 위한 무전극 UV Lamp의 개발

신동호,이규현,이용택 한국공업화학회 2003 응용화학 Vol.7 No.2

하수방류수 살균소독에 이용되는 UV 램프를 기존의 전극용보다 효율을 높이고 수명이 긴 무전극 UV 램프를 제작하고 그 성능을 알아보았다. 우선 활성물질을 변화시키면서 제조한 램프의 UV강도 및 온도의 변화를 살펴보았다. 그 결과 활성물질이 Hg/Ind의 무게비 95/5로 만든 램프를 250분간 운전한 결과 UV 강도 300 mW/㎤ 및 표면온도 200-250 ℃로 가장 안정적인 결과를 가져왔다. 그러나 무전극 램프를 장시간 발광시켰을 경우 램프의 표면온도가 상승하기 때문에 실제 하수처리공정에 사용하기 위하여 냉각이 가능한 이중관 형태로 제작하여 UV강도와 온도 변화특성도 알아보았다. 또한 제작된 무전극 UV lamp를 하수 방류수의 살균소독을 위하여 대장균(E-coli.)으로 실험한 결과 99.9% 이상의 살균효율을 보였다.