재래한우의 보존을 위한 혈청 및 혈구단백질의 유전적 다형현상

한상기(S . K . Han),윤희섭(H . S . Yoon),정의룡(E . Y . Chung),신유철(Y . C . Shin),변희대(H . D . Byun) 한국축산학회 1995 한국축산학회지 Vol.37 No.1

Biochemical polymorphisms of five red cell and semen proteins, Hemoglobin(Hb), Transferrin(Tf), Post-transferrin 2(Ptf2), Post-albumin(Pa) and Albumin(Alb) as genetic markers in Korean cattle were analyzed by Starch and Polyacryamide gel electrophoresis and their phenotypes, genotypes and gene frequencies were estimated in order to analysis the genetic constitution of Korean native cattle population. In the Hemoglobin(Hb) locus four different phenotypes AA, AB, BB and CH were observed and assumed to be controlled by four different alleles designated Hb^A, Hb^B, Hb^C and Hb^H, and the Hb^H type was rare variant of Korean native cattle. The observed distribution of phenotypes were 73.37% for AA type, 23.37% for AB type. 2.72% for BB type and 0.54%r for CH type. Gene frequencies of Hb^A, Hb^B, Hb^C and Hb^H were 0.8505, 0.1440, 0.0027 and 0.0027. Semen Transfetrin(Tf) locus, 11 different phenotypes AA, AD₁, AD₂, AE, AH, D₁D₁, D₁D₂, D₁E, D₂H, D₂D₂, D₂E, EE and EH type were identified, which considered to be controlled by codominant alleles TF,^A Tf^D, Tf^D, Tf^E and Tf^H at a single locus. The frequencies of Tf genotypes AD₁, D₁E, D₁D₂, D₂E, AA, AE, D₁D₂, AD₂, D₁D₁, EE, AH, D₂H and EH were found to be 16.30, 13.33, 11.85, 10.37, 9.69, 8.15, 7.41, 9.63, 5.93, 4.44, 1.48, 0.74 and 0.01%, respectively. Gene frequencies of TF^A, Tf^(D1) Tf^(D2) and Tf^H were 0.2741, 0.2704, 0,2333, 0.2074 and 0.0148, respectively. And TfH gene were newly identified in Korean native cattle. Considering Post-transterrin 2 locus, three different phenotypess FF, FS and SS were identified, which considers to he controlled by two alleles Ptf^F and Ptf^S at a single autosomal locus. The frequencies of Rf genotypes FS, FF and SS were found to be 51.06. 36.88 and 12.06%n, respectively and gene frequencies of Ptf^F and Ptf^S were 0.6241 and 0.3759. In the Postalbumin(Pa) locus, three different phenotypes FF, FS and SS type were observed to be genetically controllled by Pa^F and Pa^S gene. And genotypes frequencies FS. FF amd SS type were 48.65, 36.(H and 1_5.32%, respectively. The gene frequencies of Pa^F and Pa^S were 0.6036 and 0.3964. The Albumin(Alb) locus were observed to lack any individual variation. Therefore, this locus were defined to be monomorphic. In comparison of genetic distance and dendogram calculated from the gene frequencies, close relationship was obtained between the Japanese cattle and the Korean cattle.

RF 스위치 적용을 위한 박막 PZT 엑추에이터의 d<SUB>31</SUB> 구동과 d<SUB>33</SUB> 구동 특성 비교

신민재(M. J. Shin),서영호(Y. H. Seo),최두선(D-S. Choi),황경현(K-H. Hwang) 한국정밀공학회 2006 한국정밀공학회 학술발표대회 논문집 Vol.2006 No.5월

In this work, we present the comparison between d<SUB>31</SUB> and d<SUB>33</SUB> mode characterization using the PZT micro-actuator for large displacement. The PZT micro-actuator consisted of Si, PZT, and Pt layer on SOI wafer. The electrode shapes were laminated and interdigitated for d<SUB>31</SUB> and d<SUB>33</SUB> mode, respectively. In order to characterize the actuation mode, we measured the displacement using laser interferometer. The maximum displacement of d<SUB>31</SUB> mode was 12.2㎛ at 10V, the actuation characterization of d<SUB>31</SUB> was better than that of d<SUB>33</SUB> mode. We estimated that displacement of d<SUB>33</SUB> mode would be larger than that of d<SUB>31</SUB> above 30V.

Predictors of survival for patients with cancer after cryptogenic stroke

Shin, Y. W.,Lee, S. T.,Jung, K. H.,Kim, D. Y.,Park, C. K.,Kim, T. M.,Choi, S. H.,Chu, K.,Lee, S. K. M. Nijhoff 2016 Journal of neuro-oncology Vol.128 No.2

<P>Patients with active cancer experience ischemic stroke via cryptogenic mechanisms, with cancer-associated hypercoagulability being considered a major contributor to such strokes. Despite the remarkably shortened survival of these patients, the clinical predictors of survival are poorly understood. We determined the clinical factors including D-dimer levels serving as the predictors of overall survival in these patients. Retrospective study was conducted on cancer patients who visited our hospital for acute ischemic stroke with cryptogenic mechanisms from April 2012 through November 2014. Demographics, clinical characteristics, imaging and laboratory results including coagulation markers were collected, and overall survival was calculated from the patient medical records and a governmental national database. A high D-dimer level was defined as a D-dimer level exceeding the median value from the study population (> 5.50 mu g/ml). A total of 93 patients were identified, with a median survival of 62 days (interquartile range 32-223 days). A high D-dimer level (p = 0.004; hazard ratio [HR] 2.01, 95 % confidence interval [CI] 1.26-3.21), systemic metastases (p = 0.02; HR 2.08, 95 % CI 1.11-3.90), and diabetes mellitus (p = 0.03; HR 1.78, 95 % CI 1.03-3.10) were identified as independent predictors of poor overall survival using multivariate Cox proportional hazard analysis. Most of the patients (87 %) were primarily treated with low-molecular-weight heparin (dalteparin, n = 49; enoxaparin, n = 32). The type of low-molecular-weight heparin had no association with survival. A high D-dimer level, systemic metastases, and diabetes are independent predictors of poor survival in cancer patients with cryptogenic stroke.</P>

Chemical Bath Deposition 방법으로 제작한 CdSe 박막의 특성

홍광준,이상열,유상하,서상석,문종대,신영진,정태수,신현길,김택성,송정훈,유기수 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

Chemical bath deposition 방법으로 다결정 CdSe 박막을 세라믹 기판 위에 성장시킨 다음 온도를 변화시켜 열처리하고 X-선 회절무늬를 측정하여 결정구조를 밝혔다. 450℃로 열처리한 시료가 X-선 회절무늬로 부터 외삽법에 의해 a_o와 c_o 는 각각 4.302 Å과 7.014 Å인 육방정계임을 알았다. 이 때 낱알크기는 약 0.3㎛이었다. Van der Pauw 방법으로 Hall 효과를 측정하여 운반자 농도와 이동도의 온도의존성을 연구하였다. 이동도는 33 K 에서 200 K 까지는 압전산란에 의하여, 200 k 에서 293 K 까지는 극성광학산란에 의하여 감소하는 경향이 나타냈다. 광전도 셀의 특성으로 스텍트럼 응답, 감도(γ), 최대허용소비전력 및 응답 시간을 측정하였다. Polycrystalline CdSe thin films were grown on creamic substrate using a chemical bath deposition (CBD) method. They were annealed at various temperature and X-ray diffraction patterns were measured by X-ray diffractometer in order to study CdSe polycrystal structure. Using extrapolation method of X-ray diffraction patterns for the CdSe samples annealed in N_2 gas at 450℃ it was found hexagonal structure whose lattice parameters a_o and c_o were 4.302 Å and 7.014 Å, respectively. It grain size was about 0.3 ㎛. Hall effect on this sample was measured by Van der Pauw method and studied on carrier density and mobility depending on temperature. From Hall data, the mobility was likely to be decreased by piezo electric scattering at temperature range of 33 K and 200 K, and by polar optical potical scattering at temperature range of 200 K and 293 K. We measured also spectral response, sensitivily (γ), maximum allowable power dissipation and response time on these samples.

CBD 방법에 의한 CdS 박막의 성장과 광전도 특성

황광준,이상열,유상하,서상석,문종대,신영진,정태수,신현길,김택성,송정훈,유기수 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

Chemical bath deposition 방법으로 다결정 CdS 박막을 세라믹 기판 위에 성장시킨 다음 온도를 변화시켜 열처리하고 X-선 회절무늬를 측정하여 결정구조를 밝혔다. 550℃로 열처리한 시료의 경우 X-선 회절무늬로 부터 외삽법에 의해 a_。와 c_。는 각각 4.1364 Å과 6.7129 Å인 육방정계임을 알았다. 이 때 낱알크기는 약 0.35㎛이었다. Van der Pauw 방법으로 Hall 효과를 측정하여 운반자 농도와 이동도의 온도의존성을 연구하였다. 이동도는 33 K 에서 150 K 까지는 압전산란에 의하여, 150 K 에서 293 K 까지는 극성광학산란에 의하여 감소하는 경향이 나타냈다. 광전도 셀의 특성으로 스텍트럼 응답, 감도(γ), 최대허용소비전력 및 응답 시간을 측정하였다. Polycrystalline CdS thin films were grown on creamic substrate using a chemical bath deposition method. They were annealed at various temperature and X-ray diffraction patterns were measured by X-ray diffractometer in order to study CdS polycrystal structure. Using extrapolation method of X-ray diffraction patterns for the CdS samples annealed in N_2 gas at 550℃ it was found hexagonal structure whose lattice constants a_o and c_o were 4.1364 Å and 6.7120Å, respectively. Its grain size was about 0.35 ㎛. Hall effect on this sample was measured by Van der Pauw method and studied on carrier density and mobility depending on temperature. From Hall data, the mobility was likely to be decreased by piezo electric scattering at temperature range of 33K and 150K and by polar optical scattering at temperature range of 150K and 293K. We measured also spectral response, sensitivity (γ), maximum allowable power dissipation and response time on these samples.

Production of d-psicose from d-fructose by whole recombinant cells with high-level expression of d-psicose 3-epimerase from Agrobacterium tumefaciens

Park, C.S.,Park, C.S.,Shin, K.C.,Oh, D.K. Society for Bioscience and Bioengineering, Japan ; 2016 Journal of bioscience and bioengineering Vol.121 No.2

<P>The specific activity of recombinant Escherichia coli cells expressing the double-site variant (133L-S213C) D-psicose 3-epimerase (DPEase) from Agrobacterium tumefaciens was highest at 24 h of cultivation time in Terrific Broth (TB) medium among the media tested. The contents of crude protein and DPEase in recombinant cells at 24 h were 37.0 and 8.6% (w/w), respectively, indicating that the enzyme was highly expressed. The reaction conditions for the production of D-psicose from D-fructose by whole recombinant cells with the highest specific activity were optimal at 60 degrees C, pH 8.5, 4 g/l cells, and 700 g/l D-fructose. Under these conditions, whole recombinant cells produced 230 g/I D-psicose after 40 min, with a conversion yield of 33% (w/w), a volumetric productivity of 345 g/l/h, and a specific productivity of 86.2 g/g/h. These are the highest conversion yield and volumetric and specific productivities of D-psicose from D-fructose by cells reported thus far. (C) 2015, The Society for Biotechnology, Japan. All rights reserved.</P>

Induction of Cell Death in Human Macrophages by a Highly Virulent Korean Isolate of <i>Mycobacterium tuberculosis</i> and the Virulent Strain H37Rv

Sohn, H.,Lee, K.-S.,Kim, S.-Y.,Shin, D.-M.,Shin, S.-J.,Jo, E.-K.,Park, J.-K.,Kim, H.-J. Blackwell Publishing Ltd 2009 Scandinavian journal of immunology Vol.69 No.1

<P>Abstract</P><P>Recent studies have suggested that virulent strains of <I>Mycobacterium tuberculosis</I> induce apoptosis in macrophages less often than do attenuated strains. K-strain, which belongs to the Beijing family, is the most frequently isolated clinical strain of <I>M. tuberculosis</I> in Korea. In this study, we investigated the differential induction of cell death in human monocytic THP-1 cells by K-strain and H37Rv, a virulent but laboratory-adapted strain of <I>M. tuberculosis</I>. Although no significant difference in growth rate was observed between the cells exposed to K-strain and those exposed to H37Rv, the levels of protective cytokines such as tumour necrosis factor (TNF)-&agr;, interleukin (IL)-6 and IL-12p40 were lower in K-strain-infected cells than in H37Rv-infected cells. Cell viability assays showed that both K-strain and H37Rv, but not heat- or streptomycin-killed bacteria, induced THP-1 cell death in a TNF-independent manner. In contrast, double staining with fluorochrome-labelled inhibitors of caspase and propidium iodide and lactate dehydrogenase release assays revealed that K-strain induced significantly higher levels of necrotic cell death, rather than apoptosis, in THP-1 cells than did H37Rv. Anti-apoptotic <I>Bcl-2</I>, <I>Mcl-1</I>, <I>Bfl-1</I> and <I>Bcl-xL</I> in the cells were significantly upregulated following infection with K-strain compared with H37Rv, whereas <I>Bax</I> was slightly upregulated in response to infection with both H37Rv and K-strain. These results suggest that the highly virulent K-strain keeps cellular apoptosis as a host defense mechanism to a minimum and induces necrosis in macrophages.</P>

Characterization of Bacteriophage of Lactococcus cremoris

Shin, D. K 韓國酪農學會 1996 韓國酪農學會誌 Vol.18 No.2

Chemical Bath Deposition 방법으로 제작한 CdSe 박마의 특성

신영진,홍광준,이상열,유상하,서상석,문종대,신현길,김택성,송정훈,유기수,정태수 한국센서학회 1993 센서학회지 Vol.2 No.1

Polycrystalline CdSe thin films were grown on ceramic substrate using a chemical bath deposition (CBD) method. They were annealed at various temperature and X-ray diffraction patterns were measured by X-ray diffractometer in order to study CdSe polycrystal structure. Using extrapolation method of X-ray diffraction patterns for the CdSe samples annealed in N₂ gas at 450℃ it was found hexagonal structure whose lattice parameters a_o and c_o were 4.302 A and 7.014 A, respectively. Its grain size was about 0.3 ㎛. Hall effect on this sample was measured by Van der Pauw method and studied on carrier density and mobility depending on temperature. From Hall data, the mobility was likely to be decreased by piezo electric scattering at temperature range of 33 K and 200 K, and by polar optical scattering at temperature range of 200 K and 293 K. We measured also spectral response, sensitivity (γ), maximum allowable power dissipation and response time on these samples.

간헐포기를 이용한 고도처리공정에서 SRT와 인제거의 상관관계

신응배,주봉현,박주섭,이두진,김연권,이미경 漢陽大學校 環境科學硏究所 2001 環境科學論文集 Vol.22 No.-

생물학적 하·폐수처리에서 고형물 체류시간(Solid Retention Time, SRT)은 공정내에서 적절한 미생물량과 성장상태를 유지하기 위한 중요한 운전 인자들 가운데 하나이다. 특히 고도처리 공정에서 생물학적 인제거는 궁극적으로 잉여슬러지의 폐기를 통해 이루어지므로, 미생물의 성장률에 영향을 미치는 SRT와 폐기되는 슬러지내의 인 함유량은 매우 중요한 의미를 지닌다. 본 연구에선 lab scale의 간헐포기공정을 이용하여 SRT의 변화에 따른 인제거 효율 평가, 슬러지내 인 함유량 변화 및 미생물 분포에 대한 예측으로 SRT에 따른 인제거 특성을 살펴보았다. 반응기의 SRT를 15, 20 및 30일로 운전하여 인제거 효율을 평가한 결과, 인제거 효율은 각각 78.4%, 93.3% 및 84.0%로 나타나 SRT 20일이 가장 높았으나, 슬러지내의 인 함유량은 5.41%, 5.88%로서 SRT가 증가할수록 인 함유량이 증가하였다. SRT가 30일인 경우가 슬러지내 인 함유량이 가장 높았음에도 불구하고, SRT 20일로 운전한 경우의 인제거율이 가장 높았던 이유는 잉여슬러지로 폐기되는 인부하량이 가장 많았기 때문이다. SRT의 변화에 따라 미생물의 분포 변화를 살펴보기 위해 일반 표준활성슬러지와 SRT 15, 20, 30일로 운전된 본 반응기의 슬러지를 대상으로 호기성 소화실험을 실시하였다. 일반 표준활성슬러지는 소화실험 초기에 급격한 VSS의 감소를 보였으나, 인 방출량은 매우 적어, 슬러지내에 phosphate accumulating organisms(PAOs)가 거의 존재하지 않는다는 것을 확인할 수 있었으며, 고도처리 슬러지의 경우 SRT가 긴 슬러지 일수록 VSS의 감소율은 작아지지만, 인 용출량은 증가하여 슬러지내에 PAOs가 우점화되어 있는 것으로 조사되었다. The solid retention time (SRT) is one of the important control parameters in biological phosphorus removal. In this study, the lab scale biological nutrient removal (BNR) reactor using intermittent aeration was operated at various SRTs to evaluate the phosphorus removal efficiency. At SRTs of 15, 20, and 30 days, the phosphorus removal efficiencies were 78.4%, 93.3%, and 84.0%, respectively, giving an optimal SRT at 20 days and phosphorus contents in the biomass were 5.41%, 5.88%, and 6.26%, respectively. The amount of phosphorus removed by wasting excess sludge was estimated as 87.78 mgP/d, 114.03 mgP/d, and 90.90 mgP/d respectively. Aerobic digestion experiments were also carried out to investigate the behavior of the phosphate accumlating organisms (PAOs) in biomass produced at different reactors. An activated sludge from the BNR process showed slower volatile suspended solids (VSS) destruction rate and higher amount of phosphorus release, compared to the conventional activated sludge (CAS), suggesting the CAS had little PAOs compared to the activated sludge from the BNR process Also slower destruction rate and higher amount of phosphorus release in the sludge at 30 days SRT compared to the sludge at 15 days SRT. suggested PAOs were more predominant at longer SRT.