동결보존한 마우스 집합배의 생존성과 chimera의 생산에 관한 연구

신상태,조충호 충남대학교부설 생명공학연구소 1991 생물공학연구지 Vol.1 No.-

The present study was carried out to investigate the viability of frozen-thawed aggregated mouse embryos and to produce the chimeras. Different phenotypic embryos were obtained by mating ICR female mice with ICR or CBA male mice. The aggregated morulae were produced following aggregation of embryos at 4-, 8- and 12- to 16-cell stage. The desirable stage for the aggregation of the mouse embryos was 8- to 16-cell stage. The post-thawed in vitro survival rates of aggregated embryos in glycerol, DMSO and ethylene glycerol were 51.5, 78.6 and 69.4%, respectively. Although the higher survival was obtained in DMSO, there were no significant differences in the survival rates among the three cryoprotectants. A total of 155 frozen-thawed aggregated embryos were transferred to 11 recipient mice, 3 out of 7 offspring were born to overt chimera. These experiments have proven that mouse chimeras can be obtained from forzen-thawed aggregated embryos.

요양소에 수용중인 정신장애자의 입소전 치료방법에 관한 연구

신병호,윤진상,이충경,이형영 大韓神經精神醫學會 1988 신경정신의학 Vol.27 No.5

In Korea, a large number of the mentally ill are still institutionalized in asylums where they can not receive modern psychiatric treatments. This survey was designed to investigate previous treatment methods of these psychiatric patients and to offer basic data in order to develop modern psychiatric delivery system for patients institutionalized in asylums. The 694 subjects who could give cooperative informations wee selrected form 6 asylums which are located in the Kwang-ju and Chonnam area. The data on this survey was collected by investigator's interview from Mar. 30, 1987 to Apr. 10, 1987. The previous treatment methods were divided into 8 groups(modern psychiatric treatment, asylym, Korean shamanic rite, religious healing, herb medicine, drug store, others and no treatment) and multiple responses were allowed. The methods were studied relating to the following psychiatric and sociodemographic factors : psychiatric diagnosis, age at the time of institutionalization, morbid duration before institutionalization, sex, occupation, residence, religion, economic status, marital status, educational level, and guardian. 49% of the patients had no experienced any previous treatment for their mental illness. 35% of the patients had been given modern psychiatric treatment and 23% had been previously institutionalized in asylums. The other treatment methods were reported in less than 10% of the patients. There were significant relationships between the choice of treatment methods and the psychiatric and sociodemographic factors.

당근을 이용한 식초 및 음료수의 개발

신진한,류충호,조성환 경상대학교 농업생명과학연구원 2002 농업생명과학연구 Vol.36 No.1

당근파쇄물을 Termamyl, Dextrozyme 및 Viscozyme과 같은 당질 가수분해 효소로 가수분해시킨 당화물을 Saccharomyces cerevisiae로 발효시켜 얻어진 알코올 용액을 기질로하여 Acetobacter aceti 배양에 의하여 식초를 제조하고, 이와같은 제조한 식초를 이용하여 여러 형태의 식음료를 조제하여 관능검사를 실시하였다. 먼저, 당질가수분해효소로 당근파쇄물을 가수분해아여 환원당 농도가 0.6%이던 당근 파쇄물의 환원당 농도가 7.0%로 증가된 당근 당화물을 제조할 수 있었다. 이어서, 당근당화물에 포도당을 첨가하여 환원당 농도를 20%로 조절한 후, 알코올 발효시켜 알코올농도를 6.0%로 조절한 후, 종초(Acetobacer aceti의 전 배양액)를 가해 배지의 초기산도가 1.0% 부근이 되도록 조절하여 30℃에서 정치배양 및 진탕 배양시켰다. 진탕 배양의 경우, 발효 22일 경에 최대 산도 7.0%에 도달하였고, 정치배양의 경우에는 발효 30일 경에 최대 산도 6.8%에 도달하였다. 이와같이 조제한 당근식초를 저온살균하고 이를 이용하여 제조한 식음료로는 액상과당을 10%가량 첨가하여 제조한 음료 시제품이 당근과즙과 각종 부원료를 첨가하여 제조한 시제품보다 높은 관능 평가 결과를 얻었다. To develop vinegar and beverage products from carrots, carbohydrates in carrots were hydrolyzed with hydrolyzing enzymes such as Termamyl, Dextrozyme, and Viscozyme. The hydrolysate was used as the substrates of alcohol and acetic acid fermentation by Saccharomyces cerevisiae and Acetobacter aceti, respectively. The sensory evaluation for the prepared vinegar and vinegar-containing beverages was carried out. After hydrolyzing crushed carrots, the saccharified product could be manufactured in the 7.0% ratio of deoxidized saccharification, whose concentration was originally 0.6%. After the concentration of reducing sugars was made up to 20% by adding glucose to the saccharified product, the alcohol fermentation was done at 30℃. After alcohol fermentation, 6.0% of alcohol content was composed in the liquid medium precultivated by acetobacter aceti and the acidity in the early stage was reached around 1.0%. While it arrived at the maximum acidity of 7.0% at the 22th day of fermentation in the case of shaking cultivation, the alcohol concentration in the culture was the maximum acidity of 6.8% at the 30th day of fermentation in the case of the static cultivation. In the sensory evaluation for the various kinds of beverage prepared with the carrot vinegar, the testing beverage manufactured with adding 10% of liquid fructose to the culture showed best results.

Chlorpromazine으로 유발된 음경지속발기증 1례

신유호,윤도준,이충현,송지영,이상철 大韓神經精神醫學會 1996 신경정신의학 Vol.35 No.3

저자들은 항정신병약물 chlorpromazine 치료 중에 음경지속발기증이 발생하여 수술까지 시행하여 회복된 환자 1례를 체험하였기에 문헌고찰과 함께 보고하였다. 항우울제 trazodone과 함께 chlorpromazine, thioridazine과 같은 항정신병약물이 음경지속발기증의 원인과 관련이 있는 것으로 알려지고 있다. 이러한 약물에 의해 유발되는 음경지속발기증의 기전에 대해서는 알파-아드레날린 차단으로 인한다는 설이 많은 지지를 받고 있다. 치료는 보존적인 치료와 외과적인 치료가 있으며, 외과적인 치료로는 음경해면체 천자와 흡입 세척술 그리고 분로를 만들어 주는 방법들이 있다. 음경지속발기증은 신속한 진단과 치료를 하지 않을 경우 영구적인 발기불능을 초래할 수도 있는 부작용이므로 향정신약물 투여시 이에 대한 관심과 주의를 가져야 할 것이다. Antipsychotic drugs can induce several sexual side effects. Priapism, one of the side effects, is defined as "the persistent abnormal erection of the penis, which usually occurs without sexual desire". There has been an increasing number of reports recently linking the etiology of priapism with psychotropic medication. The drugs known to be associated with priapism are psychotropic drugs such as trazodone, chlorpromazine, thioridazine as well as antihypertensives, anticoagulants and so on. The mechanism of a drug which induces priapism is proposed to be mediated by its alpha-adrenergic blocking effect. Prompt diagnosis and treatment are essential. Priapism is a severe side effect, which can lead to impotence if prompt diagnosis and treatment are not carried out. There are medical and surgical treatments for priapism. The surgical treatments usually involve aspiration, irrigation and the creation of shunt. We report a case of priapism which developed after two years of chlorpromazine treatment. He is a 20-year-old man with schizophrenia. He experienced two times of brief episode of prolonged penile erection before developing intractable priapism. He was treated by shunt operation which showed satisfactory relief of priapism. It seemed by this case that duration and dosage of chlorpromazine were not closely related with priapism. Psychiatrist is needed to give attention to the priapism as one of sexual side effects due to antipsychotics though it is not common.

한국산 독말풀에 대한연구(Ⅰ) : HPLC에 의한 Hyoscyamine 과 Scopolamine의 정량

도경삼,신민교,송호준,선중기,손인경,백승화 원광대학교기초자연과학연구소 1992 基礎科學硏究誌 Vol.11 No.3

A new method for quantitative determination of Hyoscyamine and Scopolamine in Datura stramonium L. by High Performance Liquid Chromatography(HPLC) was established. A reversed-phase system with a μ Bondapak C18 column using methanol in 0.2% aqueous phosphoric acid(40:60) with PIC A as a mobile phase was developed. Hyoscyamine and Scopolamine were detected at 254nm and the analysis was successfully carried out within 20min.

양극성 기분장애에서 세로토닌 수용체 유전자의 다형성(102T/C)에 관하여

지익성,신석철,왕성근,이선우,신용재,김충수,김상국,최하석,오승용,류택근,이영호 대한생물치료정신의학회 1999 생물치료정신의학 Vol.5 No.2

만성 간장 질환자에서 뇌 MRI 상의 pallidal index와 생물학적 망간지표들 간의 관련성

최영희,박능화,신정우,김효경,김성률,정태흠,박지강,이헌,유철인,이충렬,이지호,김양호 大韓産業醫學會 2004 대한직업환경의학회지 Vol.16 No.2

목적 : 만성 간장 질환자를 대상으로 T1강조 자기공명영상(MRI)에서 나타나는 담창구 부위의 고신호강도 소견(pallidal index, PI)과 생물학적 망간 지표들 간의 관련성을 알아본다. 방법 : B형간염 바이러스에 의한 간경변증으로 진단 받은 40세 이상의 환자 31명과 정상 대조군 10명을 대상으로 설문조사와 혈액 및 소변검사, 뇌 MRI를 시행하였다. 전체 41명 가운데 검사 전 수혈을 받았던 환자군 9명을 제외하고 전체 32명의 자료를 분석하였다. 망간 축적에 관련된 객관적 지표로서 T1 강조 MRI에서 나타나는 담창구 부위의 고신호강도를 전두부 백색질 신호강도로 나눈 수에 100을 곱하여 구한 PI를 택하였다. 환자군과 대조군 사이의 PI와 망간농도들의 평균을 비교하였다. 여러 변수들과 PI와의 상관성을 보기 위해서는 상관분석을 시행하였다. 결과 : 간장 질환자 22명 가운데 18명(81.8%)에서 뇌 MRI 상 고신호강도가 나타났다. 대조군 10명에서는 아무도 MRI 소견상 고신호강도가 나타나지 않았다. 간장 질환자에서의 평균 PI는 122.62로 대조군의 106.23보다 유의하게 높게 나타났다(p<0.01). 간장 질환자의 전혈에서 망간농도는 2.34㎍/㎗으로 대조군의 1.44㎍/㎗와 비교하여 유의하게 높게 나타났다(p<0.05). 혈장과 소변 내 망간농도는 대조군에서 다소 높게 나타났으나, 통계적으로 유의하지는 않았다. 또한 혈색소, 빌리루빈, AST가 대조군과 환자군 사이에서 유의한 차이가 있었다(p<0.05). 전혈(p=0.489, P<0.01, n=29)과 적혈구(p=0.573, P<0.01, n=22) 내 망간농도와 PI 사이에는 각각 유의한 상관관계가 있었다. 그러나 혈장과 소변 내 망간 농도와 PI와의 상관관계는 통계적으로 유의하지 않았다. 총빌리루빈 또한 PI와 유의한 상관관계를 나타내었다(p=0.509, P<0.01, n=32). 결론 : 전혈 또는 적혈구 내의 망간 농도가 뇌 내 망간 침착으로 인한 T1 강조 MRI상 담창구 부위의 고신호 강도를 잘 반영하는 유용한 지표이다. Objectives: The objectives of this study were to clarify which biological manganese exposure indices reflect the pallidal signal intensities in magnetic resonance imaging (MRI) in liver cirrhotics. Methods: We examined whole blood, plasma, RBC and urinary manganese concentrations, as well as, brain MRI in 22 cirrhotic patients and 10 healthy controls. From MRI scans we calculated the signal intensity of the globus pallidus using the pallidal index (PI), the ratio of the globus pallidus to subcortical frontal white-matter signal intensity in axial T1 -weighted MRl planes multiplied by 100. In addition, we studied the relationships between PI and other measurements. Results: The high signal intensity in the globus pallidus on T1-weighted MRI was observed in 18 (81.8%) patients. There was a significant correlation between whole blood and RBC manganese concentration, and PI on MRI. According to multiple linear regression, whole blood and RBC manganese concentration reflected PI on MRI better the other indices did. Conclusions: Whole blood and RBC manganese concentrations could be useful as biological manganese exposure indices that reflect PI on MRI.

한국 정신장애의 역학 조사 연구[I] : 각 정신장애의 유병률

조맹제,함봉진,김장규,박강규,정은기,서동우,김선욱,조성진,이준영,홍진표,최용성,박종익,이동우,이기철,배재남,신정호,정인원,박종한,배안,이충경 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.4

Objectives : This study aims to estimate the prevalence of the DSM-IV psychiatric disorders in Korean population using the Korean version of Composite International Diagnostic Interview (K-CIDI). Methods : Subjects were selected by taking multi-stage, cluster samples of 7,867 adult household residents, 18 to 64 years of age, in ten catchment areas. Total 78 trained interviewers administered the K-CIDI to the selected respondents, from June 1 to November30,2001. Results : Total 6,275 respondents completed the interview. Some 33.5% of respondents reported at least one lifetime disorder, 20.6% reported at least one-year disorder, and 16.7% reported at least one-month disorder. The most common lifetime disorders were alcohol abuse/dependence (17.24%), nicotine dependence/withdrawal (11.19%), specific phobia (5.16%), and major de-pressive disorder (4.25%). The lifetime prevalence of substance abuse/dependence (0.25%) and schizophrenia (0.16%) was very low. Nicotine and alcohol use disorder showed very high male/female ratio. Mood disorder and anxiety disorder were more prevalent among female than male. Conclusion : The prevalence of psychiatric disorders was high. In comparison with other studies, remarkable differences in distributions of psychiatric disorders across the areas and times were observed.

An Optimal Method to Improve the Visual Quality of Medical Images

Choong-ho Shin, Chai-yeoung Jung 조선대학교 기초과학연구원 2015 조선자연과학논문집 Vol.8 No.2

As the visual quality of X-ray images is a critical reference for the accuracy of the clinical diagnosis, the methods to improve the quality of X-ray images have been investigated. Among many existing methods, using frequency domain filter is a very powerful method to improve the visual quality of images. In this paper, the inherent noises of the input images are suppressed by adding the Laplacian image to the subjected image. The medical X-ray images using the optimal high pass filter has shown improved edges. Further, the optimal high frequency emphasis filter has shown the improved contrast of flat areas by using the result image from the optimal high pass filter. Also the resulting images of the global contrast have improved by the histogram equalization. As a result, the proposed methods have shown enhanced contrast and edges of the images with noise canceling effect.

An Optimal Algorithm for Enhancing the Contrast of Chest Images Using the Frequency Filters Based on Fuzzy Logic

Shin, Choong-Ho,Jung, Chai-Yeoung The Korea Institute of Information and Commucation 2017 Journal of information and communication convergen Vol.15 No.2

Chest X-ray image cannot be focused in the same manner as optical lenses and the resultant image generally tends to be slightly blurred. Therefore, appropriate methods to improve the quality of chest X-ray image have been studied in this paper. As the frequency domain filters work well for slight blurring and moderate levels of additive noises, we propose an algorithm that is particularly suitable for enhancing chest image. First, the chest image using Gaussian high pass filter and the optimal high frequency emphasis filter shows improvements in the edges and contrast of the flat areas. Second, as compared to using histogram equalization where each pixel of chest image is characterized by a loss of detail and much noises, in using fuzzy logic, each pixel of chest image shows the detail preservation and little noise.