Modulation of the Specific Interaction of Cardiolipin with Cytochrome c by Zwitterionic Phospholipids in Binary Mixed Bilayers : A2H - and 31P - NMR Study

Shim, Yoon Bo,Park, Jang Su,Kang, Shin Won,Kim, Andre,Jeong, In Chul 한국생화학회 1978 Vol. No.

The interaction of cytochrome c with binary phospholipid mixtxres was investigated by solid-state ²H- and ^(31)P-NMR. To examine the effect of the interaction on the glycerol backbones, the glycerol moieties of phosphaddylcholine (PC), phosphafidylethanolamine (PE), phosphatidylglycerol (PG), and cardiolipin (CL) were specifically deuterated. On the binding of cytochrome c to the binary mixed bilayers, no changes in the quadrupole splittings of each of the components were observed for the PGPG, PE/CL and PFJ PG 6posomes. In contrast, the splittings of CL decreased on binding of protein to the PGCL liposomes, although those of PC did not change at all. This showed that cytochrome c specifically interacts with CL in PGCL bilayers, and penetrates into the lipid bilayer to some extent so as to perturb the dynamic structure of the glycerol backbone. This is distinctly different from the mode of interaction of cytochrome c with other binary mixed bilayers. In the ^(31)P-NMR spectra, line broadening and a decrease of the chemical shift anisotropy were observed on the binding of cytochrome c for all binary mixed bilayers that were examined. These changes were more significant for the PG CL bilayers. Furthermore, the line broadening is more significant for PC than for CL in PGCL bilayers. Therefore, it can be concluded that with the polar head groups, not only CL but also PC are involved in the interaction with cytochrome c.

The Relation between Histopathologic Findings on Surgical Specimen and Outcomes in Patients with N2 Positive Stage IIIA Non-Small Cell Lung Cancer Receiving Preoperative Concurrent ..

Bo Kyong Kim,임도훈,Won Park,Joungho Han,Keunchil Park,Kwan Min Kim,김진국,Young Mog Shim,Yong Chan Ahn,Kyoung Ju Kim 대한암학회 2003 Cancer Research and Treatment Vol.35 No.6

Purpose: To evaluate the prognostic implication of histopathologic findings on the surgical specimens of N2 positive stage IIIA non-small cell lung cancer (NSCLC) patients who were treated with preoperative concurrent radiochemotherapy (CRCT) and surgery.Materials and Methods: From May 1997 to April 2000, 48 patients with N2 positive stage IIIA NSCLC were treated with preoperative CRCT and surgery. Retrospective analyses were performed on 33 patients who underwent surgical resection. The thoracic radiation therapy (TRT) dose was 45 Gy over 5 weeks with a 1.8 Gy daily fraction using 10 MV X-rays. Chemotherapy consisted of two cycles of intravenous cisplatin (100 mg/m2, on days 1 and 29) and oral etoposide (50 mg/m2/day, on days 1?? 14 and 29??42), concurrently delivered with TRT. Surgery was performed around 4 weeks of the completion of CRCT. The median follow up was 18 months. The histopathologic findings, including the proportions of viable tumor cells, fibrosis, and necrosis, as well as the tumor and nodal statuses on the surgical specimens following the preoperative CRCT, were analyzed.Results: The 3-year overall survival, disease-free survival, and local control rates were 46.1%, 49.5%, and 85.5%, respectively. Post-surgical stages decreased in 18 patients (54.5%), including 3 pathologic complete responses, were unchanged in 13 (39.4%), and increased in two (6.1%). On univariate analyses, the low proportion of the viable tumor cells was the only factor favorably affecting the overall survival rate (p=0.0386), and the histologic type of squamous cell carcinoma was a favorable factor affecting disease free survival rate (p=0.0452). On multivariate analyses, however, no factor affected the overall survival, disease free survival, or local control rates.Conclusion: The histopathologic findings of the proportion of viable tumor cells, fibrosis, and necrosis on the surgical specimens following preoperative CRCT had few prognostic implications on uni-and multi-variate analyses.Furthermore, the primary tumor and nodal responses to preoperative CRCT did not influence the outcomes. Longer- term follow-up with a larger number of patients, however, is awaited. (Cancer Research and Treatment 2003; 35:497-501)

Electrochemical Oxidation of Benzidine and Hydrazobenzene

Won Mi-Sook,Shim Yoon-Bo,Park Su-Moon Korean Chemical Society 1992 Bulletin of the Korean Chemical Society Vol.13 No.6

The electrochemistry of benzidine and hydrazobenzene was studied in water-acetonitrile mixed solutions at various pHs and the results are reported. The cyclic voltammetric peak for the oxidation of benzidine shows a pH dependency of -62 mV/pH in the pH range of 0-3.5, no pH dependency between pH values of 3.5 and about 10.5, and of about -50 mV/pH between pH=10.50 and 14.0, indicating that oxidation mechanisms differ depending on the pH of the medium. However, the CV peak for the hydrazobenzene oxidation is shown to be independent of pH of the medium, suggesting that the proton is not involved in the rate limiting step of the electrochemical oxidation of hydrazobenzene to azobenzene. Results of in situ spectroelectrochemical experiments indicate that the oxidation products obtained during the oxidation of benzidine and hydrazobenzene depend on the result of dynamic equilibria taking place at various pHs.

The Cathodic Reduction of N'-Phenyl-N-Alkyl-N-Nitrosoureas

Won Mi-Sook,Kim Jack C.,Shim Yoon-Bo Korean Chemical Society 1992 Bulletin of the Korean Chemical Society Vol.13 No.2

Porosity effect of 3D-printed polycaprolactone membranes on calvarial defect model for guided bone regeneration

Shim, Jin-Hyung,Jeong, Jae-hyang,Won, Joo-Yun,Bae, Ji-Hyeon,Ahn, Geunseon,Jeon, Hojun,Yun, Won-Soo,Bae, Eun-Bin,Choi, Jae-Won,Lee, So-Hyoun,Jeong, Chang-Mo,Chung, Ho Yun,Huh, Jung-Bo Institute of Physics Publishing Ltd 2018 Biomedical Materials Vol.13 No.1

<P>The appropriate porosity and pore size of barrier membranes were associated with the transportation of biomolecules required for new bone formation and angiogenesis. In this study, we fabricated three-dimensional (3D)-printed resorbable polycaprolactone (PCL) membranes with different porosities (30%, 50%, and 70%) to evaluate the effective pore size for guided bone regeneration (GBR) membranes. To analyze mechanical properties and cytocompatibility, PCL membranes prepared using extrusion-based 3D printing technology were compared in dry and wet conditions and tested in vitro. The proliferation rates and pattern of fibroblasts and preosteoblasts on PCL membranes with different porosities were determined using a cell counting kit-8 assay and scanning electron microscopy. PCL membrane porosity did not affect cell proliferation, but osteogenic differentiation and mechanical properties were increased with lower porosity (30%) on day 14 (p < 0.001). Similar results were found in an in vivo calvarial defect model; new bone formation was significantly higher in PCL membranes with lower porosity (p < 0.001). These results indicate that 3D-printed PCL with 30% porosity (130 mu m pore size) is an excellent pore size for GBR membranes.</P>

Effect of irradiation on kinetic behavior of <i>Salmonella</i> Typhimurium and <i>Staphylococcus aureus</i> in lettuce and damage of bacterial cell envelope

Shim, Won-Bo,Je, Gil-Soo,Kim, Kyeongyeol,Mtenga, Adelard B.,Lee, Won-Gyeong,Song, Jeong-Un,Chung, Duck-Hwa,Yoon, Yohan Elsevier 2012 Radiation physics and chemistry Vol.81 No.5

<P><B>Abstract</B></P><P>This study evaluated effect of gamma irradiation on survival of <I>Salmonella Typhimurium</I> and <I>Staphylococcus aureus</I> on lettuce and damage of cell envelope. <I>S.</I> Typhimurium and <I>S. aureus</I> were inoculated on red leaf lettuce, and they were irradiated at 0, 0.5, 1, 1.5, 2, 2.5, and 3kGy, and the samples were then stored at 7 and 25°C for 7 days. Survival of <I>S.</I> Typhimurium and <I>S. aureus</I> were enumerated on xylose lysine deoxycholate agar and Baird–Parker agar, respectively. <I>D</I><SUB>10</SUB> value (dose required to reduce 1log CFU/leaf) was calculated, and kinetic parameters (maximum specific growth rate; <I>μ</I><SUB>max</SUB> and lag phase duration; LPD) were calculated by the modified Gompertz model. In addition, cell envelope damage of the pathogens was observed by scanning electron microscope (SEM) and transmission electron microscope (TEM). <I>D</I><SUB>10</SUB> values were 0.35 and 0.33kGy for <I>S.</I> Typhimurium and <I>S. aureus</I>, respectively. During storage at 7°C, <I>S.</I> Typhimurium and <I>S. aureus</I> had significant (<I>P</I><0.05) growth only on non-irradiated samples up to about 2.5 and 4log CFU/leaf at 0.42 and 1.28log CFU/leaf/day of <I>μ</I><SUB>max</SUB>, respectively. At 25°C, cell counts of <I>S.</I> Typhimurium and <I>S. aureus</I> on the samples irradiated at 0 and 0.5kGy increased (<I>P</I><0.05) up to 3–6log CFU/leaf. The <I>μ</I><SUB>max</SUB> of both pathogens were higher in 0kGy <I>(</I>1.08–2.27log CFU/leaf/day) and 0.5kGy (0.58–0.92log CFU/leaf/day), and LPDs ranged from 1.53 to 3.14 day. SEM and TEM observations showed that cells irradiated at 1.5 and 3kGy showed disrupted cell membrane. These results indicate that gamma irradiation could be a useful decontamination technology to improve food safety of lettuce by destroying cells of <I>S.</I> Typhimurium and <I>S. aureus</I>.</P> <P><B>Highlights</B></P><P>► Low dose of gamma irradiation destroyed cell envelope of the pathogens. ► Gamma irradiation decreased cell counts of the pathogens on lettuce. ► Gamma irradiation could be useful in improving food safety of lettuce.</P>

Stable Isotope Labeled Cytochrome c3 from Desulfovibrio vulgaris on a Defined Medium as Sole Nitrogen Source

Shim, Yoon Bo,Park, Jang Su,Kang, Shin Won,Kim, Andre 생화학분자생물학회 2001 BMB Reports Vol.33 No.6

To obtain Cytochrome c₃ labeled with a stable isotope, the conditions of cultivation and the composition of medium for DvMF were examined. The growth of DvMF was steady and reproducible under purging with N₂ and under pH control. DvMF was able to go on a defined medium without natural products. The composition of the medium containing a small amount of NH,CI as sole nitrogen source was established. Then, uniformly ^(15)N-labeled Cytochrome c₃ was obtained during the culture of DvMF in a defined medium with ^(15)NH₄Cl; it was confirmed by ¹H-^(15)N HMQC.

Effects of 3D-Printed Polycaprolactone/β-Tricalcium Phosphate Membranes on Guided Bone Regeneration

Shim, Jin-Hyung,Won, Joo-Yun,Park, Jung-Hyung,Bae, Ji-Hyeon,Ahn, Geunseon,Kim, Chang-Hwan,Lim, Dong-Hyuk,Cho, Dong-Woo,Yun, Won-Soo,Bae, Eun-Bin,Jeong, Chang-Mo,Huh, Jung-Bo MDPI 2017 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.18 No.5

<P>This study was conducted to compare 3D-printed polycaprolactone (PCL) and polycaprolactone/β-tricalcium phosphate (PCL/β-TCP) membranes with a conventional commercial collagen membrane in terms of their abilities to facilitate guided bone regeneration (GBR). Fabricated membranes were tested for dry and wet mechanical properties. Fibroblasts and preosteoblasts were seeded into the membranes and rates and patterns of proliferation were analyzed using a kit-8 assay and by scanning electron microscopy. Osteogenic differentiation was verified by alizarin red S and alkaline phosphatase (ALP) staining. An in vivo experiment was performed using an alveolar bone defect beagle model, in which defects in three dogs were covered with different membranes. CT and histological analyses at eight weeks after surgery revealed that 3D-printed PCL/β-TCP membranes were more effective than 3D-printed PCL, and substantially better than conventional collagen membranes in terms of biocompatibility and bone regeneration and, thus, at facilitating GBR. </P>

Case Report : Two Cases of Adrenal Abscesses Following Histoacryl(R) (N -butyl-2-cyanocrylate) Injection

( Bo Young Lee ),( Jae Young Jang ),( Soung Won Jeong ),( Gene Hyun Bok ),( Jeong Ho Ham ),( Joo Young Cho ),( Joon Seong Lee ),( Chan Sup Shim ) 대한소화기기능성질환·운동학회(구 대한소화관운동학회) 2011 Gut and Liver Vol.5 No.2

We report two cases of adrenal abscesses that occurred following a Histoacryl(R) (N-butyl-2-cyanocrylate) injection for variceal bleeding. Patients had been diagnosed with alcoholic liver cirrhosis and gastric varices bleeding and received a Histoacryl(R) injection for the variceal bleeding. Patients had fever and abdominal tenderness and were diagnosed with an adrenal abscess at 2 months following the Histoacryl(R) injection. One patient received open drainage and the other underwent percutaneous drainage. When a patient has previously been injected with Histoacryl(R) for the treatment of variceal bleeding and presents with fever, an evaluation for an unusual complication such as adrenal abscess is recommended. (Gut Liver 2011;5:242-244)

Comparative proteomics of the mixotrophic dinoflagellate Prorocentrum micans growing in different trophic modes

Shim, Jun-Bo,Klochkova, Tatyana A.,Han, Jong-Won,Kim, Gwang-Hoon,Yoo, Yeong-Du,Jeong, Hae-Jin The Korean Society of Phycology 2011 ALGAE Vol.26 No.1

Protein profiles of a common mixotrophic dinoflagellate, Prorocentrum micans, growing autotrophically and mixotrophically (fed on the cryptophyte Rhodomonas salina) were compared using two-dimensional gel electrophoresis (2-DE) to determine if they vary in different trophic modes. Approximately 2.3% of the detected proteins were differentially expressed in the different trophic modes. Twelve proteins observed only in the mixotrophic condition had lower pI value (<5) than the fifteen proteins observed only in the autotrophic condition (>5). When the internal amino acid sequences of five selected proteins differentially expressed between autotrophic and mixotrophic conditions were analyzed using matrix-assisted laser desorption time-of-flight (MALDI-TOF) mass spectrometry, two proteins that were specifically expressed in the autotrophic condition showed homology to glyceraldehyde-3-phosphatase dehydrogenase (GAPDH) and a bacterial catalase. Three mixotrophy-specific proteins showed homology to certain hypothetical proteins from an insect and bacteria. These results suggested the presence of certain gene groups that are switched on and off according to the trophic mode of P. micans.