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Yuan, Yong-cai,Li, Shu-cai,Zhang, Qian-qing,Li, Li-ping,Shi, Shao-shuai,Zhou, Zong-qing Techno-Press 2016 Geomechanics & engineering Vol.11 No.4
A modified grey clustering method is presented to systematically evaluate the risk of water inrush in karst tunnels. Based on the center triangle whitenization weight function and upper and lower limit measure whitenization weight function, the modified grey evaluation model doesn't have the crossing properties of grey cluster and meets the standard well. By adsorbing and integrating the previous research results, seven influence factors are selected as evaluation indexes. A couple of evaluation indexes are modified and quantitatively graded according to four risk grades through expert evaluation method. The weights of evaluation indexes are rationally distributed by the comprehensive assignment method. It is integrated by the subjective factors and the objective factors. Subjective weight is given based on analytical hierarchy process, and objective weight obtained from simple dependent function. The modified grey evaluation model is validated by Jigongling Tunnel. Finally, the water inrush risk of Shangjiawan Tunnel is evaluated by using the established model, and the evaluation result obtained from the proposed method is agrees well with practical situation. This risk assessment methodology provides a powerful tool with which planners and engineers can systematically assess the risk of water inrush in karst tunnels.
Shao-shuai Yu,Hai-yan Che,Sheng-jie Wang,Cai-li Lin,Ming-xing Lin,Wei-wei Song,Qing-hua Tang,Wei Yan,Wei-quan Qin 한국식물병리학회 2020 Plant Pathology Journal Vol.36 No.5
Areca palm yellow leaf (AYL) disease caused by the 16SrI group phytoplasma is a serious threat to the development of the Areca palm industry in China. The 16S rRNA gene sequence was utilized to establish a rapid and efficient detection system efficient for the 16SrI-B subgroup AYL phytoplasma in China by loopmediated isothermal amplification (LAMP). The results showed that two sets of LAMP detection primers, 16SrDNA-2 and 16SrDNA-3, were efficient for 16SrIB subgroup AYL phytoplasma in China, with positive results appearing under reaction conditions of 64oC for 40 min. The lowest detection limit for the two LAMP detection assays was the same at 200 ag/μl, namely approximately 53 copies/μl of the target fragments. Phytoplasma was detected in all AYL disease samples from Baoting, Tunchang, and Wanning counties in Hainan province using the two sets of LAMP primers 16SrDNA-2 and 16SrDNA-3, whereas no phytoplasma was detected in the negative control. The LAMP method established in this study with comparatively high sensitivity and stability, provides reliable results that could be visually detected, making it suitable for application and research in rapid diagnosis of AYL disease, detection of seedlings with the pathogen and breeding of diseaseresistant Areca palm varieties.
( Ruo Peng Cai ),( Yan Long Jiang ),( Wei Yang ),( Wen Tao Yang ),( Shao Hua Shi ),( Chun Wei Shi ),( Jing Tao Hu ),( Wei Gu ),( Li Ping Ye ),( Fang Yu Zhou ),( Qing Long Gong ),( Wen Yu Han ),( Guil 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.2
Recently, poly-γ-glutamic acid synthetase A (pgsA) has been applied to display exogenous proteins on the surface of Lactobacillus casei or Lactococcus lactis, which results in a surfacedisplayed component of bacteria. However, the ability of carrying genes encoded by plasmids and the expression efficiency of recombinant bacteria can be somewhat affected by the longer gene length of pgsA (1,143 bp); therefore, a truncated gene, pgsA, was generated based on the characteristics of pgsA by computational analysis. Using murine IL-10 as an exogenous gene, recombinant Lactobacillus plantarum was constructed and the capacity of the surface-displayed protein and functional differences between exogenous proteins expressed by these strains were evaluated. Surface expression of IL-10 on both recombinant bacteria with anchorins and the higher expression levels in L. plantarum-pgsA’-IL-10 were confirmed by western blot assay. Most importantly, up-regulation of IL-1β, IL-6, TNF-α, IFN-γ, and the nuclear transcription factor NF-κB p65 in RAW264.7 cells after stimulation with Poly(I:C) or LPS was exacerbated after co-culture with L. plantarum-pgsA. By contrast, IL-10 expressed by these recombinant strains could reduce these factors, and the expression of these factors was associated with recombinant strains that expressed anchorin (especially in L. plantarum-pgsA’-IL-10) and was significantly lower compared with the anchorin-free strains. These findings indicated that exogenous proteins could be successfully displayed on the surface of L. plantarum by pgsA or pgsA’, and the expression of recombinant bacteria with pgsA’ was superior compared with bacteria with pgsA.
The establishment of IB-SEM numerical method and verification of fluid-solid interaction
Wang, Jing,Li, Shu-cai,Mao, Xuerui,Li, Li-ping,Shi, Shao-shuai,Zhou, Zong-qing Techno-Press 2018 Geomechanics & engineering Vol.15 No.6
The interaction between particles and fluid was investigated by IB-SEM numerical method which is a combination of combing the spectral/hp element method and the rigid immersed boundary method. The accuracy of this numerical method was verified based on the computed results with the traditional body-fitted mesh in numerical simulation of the flow through the cylinder. Then the governing equations of particles motion and contact in fluid are constructed. The movement of the particles and the interaction between the fluid and the particles are investigated. This method avoided the problem of low computational efficiency and error caused by the re-division of the grid when the solids moved. Finally, the movement simulation of multi particles in the fluid was carried out, which can provide a completely new numerical simulation method.
박종희,박상일,황명석,채소청 釜山大學校 新藥開發硏究所 1998 藥學硏究誌 Vol.32 No.1
'Jeong Wi Dan' is Chinese patent medicine, which is used for stomachic disorder, gastric and duodenal ulcer. This medicine is in the large of pills consisting 14 kinds of powdered crude drugs. For the identification of individual ingredients in such powdery mixtures, microscopic method may advantageously be used as it requires only a small amount of specimens. In this paper, effective of this method will be exemplified by the identification of the ingredients in Jeong We Dan which contains 14 powdered crude drug ingredients.
박상일,황명석,채소청,박종희,Park, Sang-Il,Whang, Myung-Seog,Cai, Shao Qing,Park, Jong-Hee 한국생약학회 1998 생약학회지 Vol.29 No.1
'Jeong Wi Dan' is Chinese patent medicine. which is used for stomachic disorder, gastric and duodenal ulcer. This medicine is in the large of pills consisting 14 kinds of powdered crude drugs. For the identification of individual ingredients in such powdery mixtures, microscopic method may advantageously be used as it requires only a small amount of specimens. In this paper, effective of this method will be exemplified by the identification of the ingredients in Jeong We Dan which contains 14 powdered crude drug ingredients.
한지아,김진수,문전옥,채소청,박종희,Han, Jee-Ah,Kim, Jin-Soo,Moon, Jeon-Ok,Cai, Shao-Qing,Park, Jong-Hee 한국생약학회 1997 생약학회지 Vol.28 No.3
Korean folk medicine 'O-I-Pul' has been used to cure arthritis, neuralgia, leucorrhea, snake-bite, vomiting and diarrhea. With regard to the botanical origin of O-I-Pul, it has been considered to be Sanguisorba species of Rosaceae, but there has no pharmacognostical confirmation on it. Morphological and anatomical examination of Sanguisorba leaves show that 'O-I-Pul' was the leaves of S. Officinalis and S. tenuifolia var. alba.
박종희,박상일,변정석,채소청,Park, Jong-Hee,Park, Sang-Il,Pyeon, Jeong-Seok,Cai, Shao-Qing 한국생약학회 1999 생약학회지 Vol.30 No.2
'Chung Kwei Won(淸快園)' is a Chinese patent medicine, which is used for stomachic disorder, acute and chronic indigestion, dispepsia, vomiting and diarrhea in Korea. This preparation consists of 16 kinds of powdered crude drugs. For the identification of individual crude drugs in such powdery mixtures, microscopic method may be used conviniently as it requires only a small amount of specimens. In this paper, the effectiveness of this method is exemplified by the identification of the ingredients in 'Chung Kwei Won' which contains 16 powder crude drug ingredients.