한의진단명과 진단요건의 표준화 연구 II (표준화 실례) : 2차년도 연구결과 중간 보고

양기상,최선미,최승훈,안규석,박경모,박종현,김성우,신승호,정우열,전병훈,고현,김정범,신상우,김성훈,김동희,권영규,엄현섭,장혜옥 한국한의학연구원 1996 한국한의학연구원논문집 Vol.2 No.1

The diagnostic requirements were suggested and explained regarding the systems of differentiation of symptoms and signs in the second year study of standardization and unification of the terms and conditions used for diagnosis in oriental medicine. The systems were as follows; - differential diagnosis according to condition of body fluid, differentiation of syndromes according to the state of qi and blood, differential diagnosis according to relative excessiveness or deficiency of yin and yang(氣血陰陽津液辨證) - differentiation of diseases according to pathological changes of the viscera and their interrelation - analyzing and differentiating of febrile diseases in accordance with the theory of the six channels(傷寒辨證) The individual diagnosis pattern was arranged by the diagnostic requirements in the following order : another name(異名), notion of diagnosis pattern, index of differentiation of symptoms and signs(辨證指標), the main point of diagnosis, analysis of diagnosis pattern(證候分析), discrimination of diagnosis pattern(證候鑑別), a way of curing a diseases(治法), prescription(處方), herb in common use(常用藥物), diseases appearing the diagnosis pattern(常見疾病), documents(文獻調査). This study was carried out on the basis of the Chinese documents and references.

간의 배아성 육종 -부분적으로 연골육종성 분화를 보인 1예 보고-

정우회,임현이,김호근,박찬일,오기근,최승훈 대한병리학회 1992 Journal of Pathology and Translational Medicine Vol.26 No.5

음성 인식 헬스케어 시스템

신진우,조우승,조성빈,김재훈,정지은,사예지,백경동,박동규 순천향대학교 부설 산업기술연구소 2021 순천향 산업기술연구소논문집 Vol.27 No.2

As modern people's interest in health grows, wearable healthcare-related technologies that can collect biometric information and receive health-related services regardless of time and place are developing. This paper proposes the healthcare system based on voice recognition function. Unlike conventional wearable healthcare systems, the proposed system provides real-time telemedicine and diagnosis and the direct connection with doctors is possible through it. And almost all functions of the application in it are operated with voice commands to lower user access barriers. Because it deals with personal information including medical information, encryption algorithms have been applied between all data movements. Finally, the healthcare system proposed in this paper is expected to increase convenience in health care and reduce the medical gap.

mecA 양성 메티실린 내성 포도구균의 mec 조절유전자에 대한 분자역학적 연구 및 다양성 분석

우희연,이남용,맹성호,한승훈,인경수,김상욱,성승용,김익상,최명식 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.3

목적 : 병원 감염과 지역 감염의 원인균으로서의 중요성이 증가되고 있는 MRSA와 MRCNS의 메티실린 내성 관련 유전자에 대해 분자역학적인 조사를 시행하고 mec 조절유전자의 다양성을 분석하여 분자유전학적 연구와 감염 관리에 기본적 자료를 제공하고자 연구를 시행하였다. 방법 : 본 연구에서는 국내 동일 대학병원에서 분리된 mecA 양성 MRSA 78균주와 MRCNS 36균주를 대상으로 항균제 감수성 검사와 중합효소연쇄반응을 시행하여 표현형과 유전형의 분포를 알아보고 내성 정도와 유전형의 상관성을 분석하였다. 결론 : 최소억제농도를 측정하여 oxacillin 감수성 양상을 알아본 결과 총 90개의 MRSA 임상검체 중 3균주에서 감수성을 보였고, MRCNS 36균주는 모두 내성을 보였으며, 고도 내성을 보이는 균주일수록 다제내성을 보이고 있었다. 중합효소연쇄반응으로 mecA 유전자 유무를 분석하여 mecA 양성을 보인 MRSA 78균주와 MSCNS 36균주 만을 대상으로 mec 조절유전자에 대한 중합효소연쇄반응을 시행하여 유전형의 분포를 조사하였다. 그 결과 MRSA와 MRCNS 모두에서 mecA-Pr-mecA-mecR1-mecI형, mecA-Pr-mecA-mecR1형과 mecA-Pr-mecA-mecR-1의 5′말단형의 3가지 유전형이 공통적으로 관찰되었는데, 이외에 MRSA에서는 mecA-mecR1형, mecA-mecR1의 5′말단형이, MRCNS에서는 mecA-Pr-mecA형과 mecA형이 추가로 분리되었다. 유전형의 분포를 연도별로 분석한 결과 연도별로 우세한 유전형이 서로 다른 것을 알 수 있었다. 유전형과 내성 정도의 상관성을 분석한 결과, mecA-Pr-mecA-mecR1을 보유한 MRSA의 경우 mecA 억제유전자로 생각되는 mecI 유전자 유무에 관계없이 모두 고도 내성을 나타내어 mecI의 유무는 내성 정도와는 일정한 관련이 없었으나, MRSA와 MRCNS 모두에서 mecI와 동시에 mecR1의 3′말단이 결혼된 유전형이 저도 및 중등도 내성을 보이는 균주에서 유의하게 많이 관찰되어 mecI가 결손된 균주에서는 mecR1의 3′말단부위의 유무가 내성 정도의 결정에 중요한 역할을 할 것으로 생각하였다. 결론 : 국내에서 분리되는 메티실린 내성 포도구균의 유전형의 분포가 국외 분리주와 차이를 보이는 것으로 관찰되었다. 또한 기존의 보고와는 달리 메티실린 내성 정도와 유전형 사이에 유의한 연관성이 있는 것으로 관찰되어 mec조절유전자가 내성 발현 뿐만 아니라 내성 정도의 결정에 중요한 역할을 하는 것으로 관찰되었다. MRSA가 중요한 감염균임에도 불구하고 아직까지 MRSA의 내성기전에 대해 확실하게 규명되지 않아 치료가 더욱 어려워지고 있는 실정이므로 이에 대한 더욱 종합적인 연구와 동시에 발생과 확산을 막기 위한 노력이 필요할 것으로 생각된다. Background : High prevalence of methicillin resistance has been noticed in staphylococci which also have been recognized as important nosocomial and multi-drug resistant pathogens. In this study, we investigated the distribution of mec regulator genes and the presence of the mutation in mecIgene to reveal the resistance mechanism at molecular level. Methods : The isolates included 90 clinical isolates of MRSA, 36 MRCNS of which methicillin resistance were determined by disk diffusion test and isolated in a single hospital during 1996-1999. We performed microdilution MIC test for oxacillin resistance and Kirby-Bauer test for other antibiotics. Genotypes of mecA positive (determined by PCR) isolates (78 MRSA, 36 MRCNS) were investigated by PCR amplification of mec regulator genes such as 3' end and 5' end of mecR1. mecI, and Pr-mecA. Results : In MIC assay, all MRSA strains except 3 strains and all MRCNS strains showed oxacillin resistance. The level of resistance correlated to the multi-drug resistance. Seven genotypes were observed in mecA positive MRSA and MRCNS. Differences of predominant genotypes among years isolated and among species were observed. Deletions of the 3' end of mecR1 and the mecI were more frequently observed in the low- and borderline-level resistant MRSA and MRCNS (P(0.05). So the important role of 3' end of mecRI in determining resistance level was suggested. Conclusion : This study demonstrated that genomic distribution of methicillin-resistant staphylococci isolated in Korea was different from that of strains isolated in other countries. And important role of mec regulator genes for expression of methicillin resistance was suggested.

木連屬 植物의 花粉發芽에 관한 硏究

金薰植,李承雨 경희대학교 부설 식량자원개발연구소 1989 硏究論文集 Vol.10 No.-

Research was conducted to investigate the effects of media composition on pollen germination and pollen tube growth in Magnolia spp. Boron remarkably promoted the pollen germination and pollen tube growth. However, no pollen germination occurred on the media containing no boron in M. sieboldii and M. obovata. The highest capacity for pollen germination and pollen tube growth was obtained with the combination of 10% sucrose and l00ppm boron. On the other hand, K, Ca, and Mg did not promote the pollen germination and pollen tube growth in both species. For the pollen tube growth, however, M. denudata showed higher sensitivity than M. liliflora to medium acidity. In M. denudata, low GA_(3) concentration in the medium hastened the pollen germination, while pollen tube growth was retarded by high GA_(3) concentration. In contrast to GA_(3), no pollen germination occurred on the medium containing 50ppm and 100ppm IBA. The capacity for the pollen germination was markedly higher in M. liliflora and M. denudata than in M. sieboldii and M. obovata.

반도체 레이저의 이득 스위칭에 의한 초단 펄스 발생

이상훈,명승일,이명우,서동선,소대화 명지대학교 대학원 1997 대학원논문집 Vol.1 No.-

반도체 레이저의 이득 스위칭에 의한 초단 펄스 발생에 대해 체계적으로 연구하였다. 즉, 동작 파라메터인 DC 바이어스 및 RF 정현파 전력에 따른 초단 이득 스위칭된 출력 펄스의 특성을 조사하였다. 최적의 펄스는 문턱 전류 부근의 DC 바이어스에서 얻어졌으며, RF 전력이 클수록 이 최적의 DC 바이어스는 감소하였다. 출력 펄스 폭은 RF 전력이 증가할수록 DC 바이어스의 변화에 덜 민감하게 나타났다. We study systematically on short pulse generation from a semiconductor laser by gain-switching. We examine the dependence of gain-switched pulse characteristics on the operating parameters, such as DC bias and RF power. The optimum short pulses are obtained around threshold DC bias. As the RF power increases, The DC bias to show shorter pulse width decreases slightly and the pulse width becomes less sensitive to the variations of the DC bias.

홍모기 (Culex pipiens pallens)에 있어서 trypsin의 분리 및 특성에 관한 연구

김상우,이승훈,성기창 성균관대학교 기초과학연구소 1992 論文集 Vol.43 No.2

Culex pipiens 모기에 있어서 흡혈 후 중장내에서 생성되는 단백질 분해효소인 trypsin의 특성에 대하여 조사하였다. 중장내의 trypsin의 활성을 조사한 결과 적정 pH는 8.1로 나타났다. 무기이온의 영향을 조사하였을 때 칼슘이온의 경우 50mM 농도에서 활성을 가장 높게 증가시켰으며, 마그네슘이온의 경우는 활성도를 미세하게 증가하거나 영향을 나타내지 못하였고, 아연이온에 의하여는 그 활성이 저해되었다. 흡혈 후 trypsin 활성은 20시간후부터 증가하기 시작하여 30시간에 최대의 활성을 나타내었고 그 후 감소하였다. Anion-exchange chromatography를 이용하여 흡혈 후 30시간 지난 중장내의 trypsin을 부분 분리한 결과 trypsin 활성을 나타내는 2개의 peak(PⅠ, PⅡ)가 존재하는 것으로 나타났다. 흡혈 후 24시간된 모기의 중장에서는 2종류의 단백질 peak가 나타났으나 PⅠ의 부위에서만 활성을 나타내었다. 흡혈 후 30시간이 지난 중장에서 활성이 나타나는 PⅡ는 흡혈 후 24시간에서 30시간 사이에 PⅡ의 해당하는 zymogen이 활성화됨으로써 나타나는 것으로 추정된다. 이들의 전기영동결과 C. pipiens는 7가지의 동이원소를 가지고 있었으며 그 중 4번∼7번째가 흡혈 후 30시간된 중장의 PⅡ에서 활성이 나타났다. Differential centrifugation 방법에 의한 trypsin의 중장내 분포를 조사한 결과 trypsin이 가용성 단백질인 것으로 밝혀졌으며 이 사실에서 microvilli로부터 중장의 강 내부로 분비되어 소화작용을 일으키는 것으로 추측된다. 이런 실험의 결과는 trypsin이 microvillar endoperitropic membrane을 통과하는 70,000 dalton 이하의 분자량을 갖음을 간접적으로 증명하고 있다. Trypsin, protease, in the midgut after bled meal from the mosquito, Culex pipiens pallens female has been examined. In the crude homogenate, the trypsin showed optimum activity at pH 8.1. The activity was stimulated by Ca^2+ at 50mM, slightly or no affected by Mg^2+, inhibited by Zn^2+. The trypsin activity in the adult females began to rise at. 20 hours after blood meal and reached at maximal level at 30 hours and started to declease. The result of anion -exchange(DEAE-cellulose) chromatography, trypsin has been found two active peaks(PⅠ, PⅡ) at the 30 hour female after blood meal. But at the 24 hour female after blood meal had two protein peaks, while only peak(PI) showed trypsin activity. The PII activity, showed in the midgut at 30 hour after blood meal, was supposed activation of zymogen PII during 24 hour to 30 hour. The result of electrophoresis, C. pipiens had seven isozymes and the 4-7th isozyme activity were appeared at PII fraction of 30hr midgut after blood meal. The distribution of trypsin in the midgut was investigated by differential centrifugation method. The result suggested that trypsin was secreted from microvilli to luminal space of midgut and the digestion was processed in the luminal space and the molecular weight was lower than 70,000 delton.

마이크로 드릴링 M/C에 의한 미세구멍가공특성에 관한 연구

민승기,이동주,이응숙,강재훈,김동우 한국공작기계학회 2001 한국공작기계학회 춘계학술대회논문집 Vol.2001 No.-

Recently, the trends of industrial products grow more miniaturization, variety and mass production. Micro drilling which take high precision in cutting work is requested more micro hole and high speed working. Especially, Micro deep hole drilling is becoming more important in a wide spectrum of precision production industries, ranging from the production of automotive fuel injection nozzle, watch and camera parts, medical needles, and thick multi-layered Printed Circuit Boards(PCB) that are demanded for very high density electric circuitry. This paper shows the tool monitoring results of micro drill with tool dynamometer. And additionally, microscope with built-in monitor inspection show the relationship between burr in work piece and chip form of micro drill machining.

딜티아젬 함유 코아 펠렛으로부터 약물의 용출에 미치는 폴록사머 함량의 영향

이승우,감성훈,홍지웅,최기송,박은석,지상철 한국약제학회 2002 Journal of Pharmaceutical Investigation Vol.32 No.4

In order to evaluate the effect of poloxamer 407 content on the dissolution profiles of pellets, diltiazem HCl (DTL) core pellets were prepared with poloxamer 407 (50∼90% w/w, with lactose as filler) using an extruder and a spheronizer. Any possible interaction between the drug and excipients was evaluated using DSC, IR and TLC. Dissolution tests were performed using USP basket method. In addition, scanning electron micrograph was performed to examine the surface roughness and cross sections. The release of DTL from the core pellets was decreased with increasing poloxamer 407 content. Cracks appeared on the surface of the core pellets with increasing the poloxamer 407 content, which may play a role on the retardation of the release of DTL from core pellets. There was no any significant interaction between the drug and excipients employed to prepare the core pellets.

西歐 代替醫學의 癌 治療에 關한 硏究

兪和承,李容淵,徐祥熏,崔宇鎭,曺政孝,李淵月,孫彰奎,趙鍾寬 대한한방종양학회 2000 대한한방종양학회지 Vol.6 No.1

At here, we investigated the historical background, current stage, and concrete therapies of altematives in cancer therapy. We obatined the conclusion below. 1. Alternative medicine(AM) is a unothodoxial medical physiology, medical practice or intervension that overcome the side effects. faults, limits of coventional medicine. 2. Recentely, as emerging New-age movement, collgpse of Decarte's mecanical cosmology, and emphasis on patient rights, the interest of AM is increased, and foreign & domestic use rates of AM are risen. 3. After coventional medicine in cancer therapies tha depends on poerations, radiations, and chemotherapy is bounded, Altematives in cancer therapy are rapidly developed, and the majority of users are often considered satisfactory. 4. Alternatives in cancer therapy are classified six boundaries; Diet and Nutrition, MInd-body Technique, Integrated System, Pharmacologic and Biologictreatments, Immuno Enhanced Therapy, Immuno Enthaced Therapy, Natural and herbal medicine. 5. Looking at contents of Alternatives in cancer therapy, we get to know most of them are similar to the treatment of oriental medicine in holistic method, therapy, philosophy. If we synthesize the alternative medicine's treatment mentioned above and investigate it with oriental medical viewpoint, that may help us approach the conquest of cancer and improve the rate of cure. that may help us approach the conquest of cancer and improve the rate of cure. 6. It is certain that foreign AM will be imported reversely, and we must prepare for rebuilding of Korean traditional AM systemically, investing for activity positively, making national policy for medical system.