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곰팡이에서 분리한 Helicobacter pylori 항균물질의 분리 및 구조규명
남궁준,연승우,백남수,김태한,김영호,김창진,김기원 충남대학교 약학대학 의약품개발연구소 1998 藥學論文集 Vol.14 No.-
Helicobacter pylori is a Gram-negative bacterium which causes chronic gastritis and is associated with gastric ulcer, duodenal ulcer and gastric carcinoma. In the process of screening of antibacterial activities against H. pylori from soil microorgani는, fungus No. 60686 was isolated. After fementaion of No. 60686, the antibacterial compound was isolated, purified and followed by extraction of mycelium with orgainic solvents, acetone and ethyl acetate, through silica gel chromatography, LH-20 gel chromatography and HPLC. As a result of the structural analyses of the compound by IR, ^1H- and ^13C-NMR, FAB/Mass spectrophotomer, the compound having the antimicrobial activity was idenfitied as chaetoglobosin A (C_32H_36N_2O_5), a cytochalasan derivative. The antimicrobial activity of chaetoglobosin A was tested against Gram-positive and negative bacteria by paper disk method. Among the test strains of 9 Gram-positive bacteria and 18 Gram-negative bacteria containing 4 H. pylori strains, the growth of 4 H. pylori strains and 3 S. aureus strains (SG 511, 285 and 503) was only inhibited by chaetoglobosin A. Also it was shown that its growth inhibition against H. pylori strains was stronger than that against S. aureus strains at the treatment of the same concentration. Therefore it was concluded that chaetoglobosin A has a specific growth inhibition against H. pylori of the tested bacteria.
1-MCP 처리 농도 및 1-MCP 처리된 切花의 저장기간이 切花 카네이션의 품질에 미치는 영향
김경숙,이승우,이정명 경희대학교 생명자원과학연구원 2004 硏究論文集 Vol.23 No.-
카네이션 'Desio'와 'Nelson' 절화의 장기저장 전의 1-MCP 1.0(2.0 μL·L^(-) ¹ 처리는 절화의 장기저장 및 저장 후의 수명연장, 품질 및 開花率 증진에 효과적이었다. 'Desio' 카네이션에서는 1-MCP 전처리 효과가 1주 저장한 절화에서 가장 높게 나타났으며 저장기간이 경과함에 따라서 1-MCP 전처리 효과가 감소되었다. 그러나 'Nelson' 카네이션에서는 1-MCP 전처리후 저온 저장기간이 4주까지 크게 나타나서 품종에 따른 반응성의 차이를 보여주었다. Treatment of 1-MCP at 1.0 μL·L^(-1) before long-term cold storage was effective in improving storability and extending vase life of cut 'Desio' and 'Nelson' carnations. The effect of 1-MCP pretreatment prior to a long term low temperature storage was most pronounced in 'Desio' carnation cut flowers stored for 1 week. In contrast, 'Nelson' showed the best effectiveness of 1-MCP pretreatment in 4-weeks stored cut flowers, thus strongly indicating the differential response of 1-MCP depending upon cultivars.
김봉석,이수훈,송준엽,이승우 한국공작기계학회 2005 한국생산제조학회지 Vol.14 No.1
For reliability assessment for machine tools, failure mode analyses by two viewpoints were studied in this paper. First, this study developed the reliability data analysis program, which searches for optimal failure distribution like failure rate or MTBF(Mean Time Between Failure) using failure data and reliability test data of mechanical parts in the web. Moreover, this data analysis program saves both failure data or reliability test data and their failure rate or MTBF for database establishment. Second, this paper conducted failure mode analysis through such performance tests as circular movement test and vibration testing for machine tools when reliability data is not available. A developed web-based analysis program shows correlations between failure mode and performance test result, and also accumulates all the data. These kinds of data analysis programs and stored data furnish valuable information for improving the reliability of mechanical system.
김승우,정용래,장경준 순천향대학교 부설 산업기술연구소 2004 순천향 산업기술연구소논문집 Vol.10 No.2
Most previous robots had used the wheels as means for movement. These structures were relatively simple and easy to control and this is why the method had been used until currently. However, there are many realistic problems to move from one place to another in human life, for instance, steps and edges. So we need to develop the two-legged walking humanoid robot. The 2-legged walking Robot system has been vigorously developed in so many corporations and academic circles of several countries. However, 2-legged walking Robot has been mostly studied in view of the static walk. We design a stable humanoid Robot which can walk in high-speed through the research of the dynamic walk in this paper. Especially, worldwide companies have been interested in developing humanoid robots for a long time to solve the before mentioned problems so that they can become more familiar with the human form. The most important thing, for the novel two-legged walk, is to create a stable and fast walking in two-legged robots. For realization of this movement, an optimal mechanical design of 12 DOFS, a distributed control and a parallel processing control are implemented in this paper. This paper proves that high speed and stable walking can be achieved, through experiments.
Begonia semperflorens 器內繁殖에 미치는 置床方法과 TIBA效果
金卿淑,閔炳訓,李承雨 경희대학교 부설 식량자원개발연구소 1993 硏究論文集 Vol.14 No.-
Begonia somperflorens 'Prelude Scarlet' 줄기組織의 再分化 및 生長에 미치는 NAA, TIBA 및 置床方法의 效果를 調査하기 위하여 遂行된 實驗結果를 要約하면 다음과 같다. 1. 不定芽 및 不定根의 再分化 및 伸長은 NAA 無處理區에서 가장 優秀하였다. 2. TIBA 處理에 의한 不定芽形成은 TIBA 0.01 및 0.05㎎/l에서 促進되었으며 TIBA 高濃度에서는 심하게 抑制되었다. 반면에 不定根의 形成은 TIBA 處理에 의해 抑制되었다. 3. 最長不定芽의 길이는 TIBA 無處理에서 가장 길었고 最長根은 TIBA 0.005㎎/l에서 가장 길었다. 4. 置床方法에 따른 再分化에 있어서 不定芽形成은 水平置床에서 가장 優秀하였고 不定根形成은 逆位置床이 水平置床과 垂直置床에 비해 높은 分化率을 보였는데 特히 TIBA 0.01과 0.05㎎/l에서 5培 以上의 많은 뿌리를 分化시켰다. 5. 置床方法別로 再分化된 最長길이는 水平置床은 不定芽의 生長, 垂直置床은 不定根의 生長에 가장 效果적이었고 逆位置床에서는 不定芽와 不定根의 길이가 가장 짧은 것으로 나타났다. Experiments were carried out to investigate the effects of NAA, TIBA and orientation methods on regeneration and growth in Begonia semperflorens "Prelude Scarlet" stem segments cultured in vitro. The regeneration and growth of adventitious shoot and root showed the best result without NAA in the medium. The concentrations of 0.01 and 0.05 ㎎/l TIBA promoted adventitious shoot formation, but higher concentrations considerably supressed. On the other hand, the formation of adventitious root was suppressed with all TIBA concentrations. The longest shoot length obtained without TIBA while length of the longest root did at 0.005㎎/l TIBA concentration. Adventitious shoot formation showed the best result in horizontal orientation. However, the formation of adventitious root showed higher regeneration rate in invented orientation compared with both horizontal or vertical orientation. Especially, the concentrations of 0.01 or 0.05㎎/l TIBA were regenerated adventitious root of 5 times or more in invented orientation. The growth of adventitious shoot and root was the most effective in horizontal and vertical orientation, respectively. The inverted orientation suppressed the growth of both adventitious shoot and root.
김준태,신승호,이승우,차성도 강원대학교 정보통신연구소 1998 정보통신논문지 Vol.2 No.-
The signal beam gains of 4% mol MgO doped LiNbO3 crystal were measured with two wave mixing geometry at room temperature. The experimental investigations have been carried out to obtain gain coefficients as a function two beam's incident angle 2θ, beam ratioβand the angleφof pump beam with respect to the c-axis. The experimental results are analyzed with diffusion only transport mechanism, and two beam coupling and absorption coefficient of the crystal obtained by using curve fitting method are presented.
김승우 순천향대학교 부설 산업기술연구소 1996 순천향 산업기술연구소논문집 Vol.2 No.2
This paper proposes an elliptical world-modeling method in order to secure the safety of the mobile robot from the unreliability of the ultrasonic sensors and estimate the lateral orientation of the detected objects. This method is advantageous because the total amount of data that the robot utilizes is reduced when old and new data is clustered during each sensing period and also because the objects in the environment can be recognized almost exactly. This paper also proposes a new local path planning algorithm which is divided into four different situations and guides the robot through the safe local path using data collected in elliptical from. This algorithm can help the AMR navigate by allowing it to sum up the virtual vector forces made from the ellipses in order to select a safe subgoal. This local path planning method using the elliptical world model has been proved by several navigation experiments.