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<P><B>Abstract</B></P><P>Haplotypes and allele frequencies of 17 Y-chromosomal STR loci included in the AmpF<I>l</I>STR<SUP>®</SUP> Yfiler<SUP>®</SUP> system were obtained from a sample of 1021 unrelated individuals living in 6 provinces of South Korea. A total of 938 haplotypes were observed in the 1021 individuals studied, of which 885 were unique. The overall haplotype diversity for the 17 Y-STR loci was 0.9998, and the discrimination capacity was 0.9187. We found 11 atypical alleles (null, duplicated, triplicated, and microvariant alleles), that have not previously been reported in South Korean populations. It seems that these 17 loci are useful genetic markers for forensic personal identification and paternity testing in the South Korean population.</P>
Though a great amount of chicken by-products are consumed everyday in many countries worldwide, however, no attention has been paid to the investigation of nutritional composition of these by-products. In the present work, the basic information regarding the aspects of nutritional composition of chicken by-products such as; liver, gizzard, heart, lung, crop, small intestines, cecum and duodenum was studied. Our results revealed that the approximate composition range (minimum to maximum) of these by-products was found as such: moisture 76.68-83.23%; fat 0.81-4.53%, protein 10.96-17.70% and calories 983.20-1,426.0 cal/g tissue, in which liver and gizzard had the highest protein content. Liver had higher (p<0.05) vitamin A, B1, B2, B3, B5 and B6 contents in comparison to other remaining byproducts. Total saturated fatty acids (SFA), unsaturated fatty acids (UFA), polyunsaturated fatty acids (PUFA) levels ranged between the by-products from 31.82% to 43.96%, 56.04% to 68.19%, and 18.27% to 32.05%, respectively. Remarkably, all of by-products showed desirable PUFA/SFA ratios. Furthermore, all of chicken by-products, especially liver, contained higher levels of trace elements (e.g., Fe, Mn and Zn) in comparison with those from muscle tissues published in literature. Overall, the study indicated that most of chicken byproducts examined are good sources of essential nutrients and these obtained results will be the useful information to consumers and meat processors.
<P>We have developed a web server for the high-throughput annotation of expressed sequence tags (ESTs) called pipeline for EST analysis service (PESTAS). PESTAS processes entire datasets with an automated pipeline of 13 analytic services, then deposits the data into the MySQL database and transforms it into three kinds of reports: preprocessing, assembling and annotation. All annotated information is provided to the scientist and can be downloaded through a web browser. To get more relevant functional annotation results, a curation function was introduced with which biologists can easily change the best-hit annotation information. We included a gene chip module that detects gene expression differences between libraries by comparing accession number counts from BLAST search results. PESTAS also provides access to the pathway information of KEGG, which is useful for mapping the relationships among networks of annotated enzymes, and is especially valuable for those researchers interested in biological pathways.</P>
A support vector machine (SVM) is a state-of-the-art machine learning model rooted in structural risk minimization. SVM is underestimated with regards to its application to real world problems because of the difficulties associated with its use. We aim at showing that the performance of SVM highly depends on which kernel function to use. To achieve these, after providing a summary of support vector machines and kernel function, we constructed experiments with various benchmark datasets to compare the performance of various kernel functions. For evaluating the performance of SVM, the F1-score and its Standard Deviation with 10-cross validation was used. Furthermore, we used taylor diagrams to reveal the difference between kernels. Finally, we provided Python codes for all our experiments to enable re-implementation of the experiments.
Zymomonas mobilis의 envelope에 관련된 특성을 Pseudomonas aeruginosa와 Escherichia coli를 대조군으로 사용하여 비교 검토하였다. Z.mobilis는 P.aeruginosa와 E.coli에 비해 lysozyme에 의해 쉽게 용해되지 않았으며, aminoglycosides와 양이온성 계면활성제인 polymyxin에 대한 내성이 높았다. 그러나 Z.mobilis는 대조군에 비해 sodium dodesyl sulfate(SDS)와 triton x-100에 대해 감수성이 높았다. 이러한 차이점을 분석하기 위해 이들 세균으로부터 분리한 outermembrane을 SDS-polyacrylamide gel을 사용하여 전기영동하였다. 그 결과 Z.mobilis의 outermembrane 단백질은 P.aeruginosa와 E.coli의 doutermembrane 단백질보다 상대적으로 컸다. Properties relating to the envelope of Zymomnas mobilis ATCC 31821 were evaluted as compared with those of Pseudomonas aeruginosa and Escherichia coli. Z.mobilis was more resistant than P.aeruginosa and E.coli to lysozyme hydrolysis, aminoglycosides, and cationic detergent, but was far more sensitive to detergents including sodium dodesylsulfate(SDS) and Triton X-100. In order to analyze these differences, outermembrance proteins obtained from these bacteria were compared by using SDS-polyacrylamide gelelectrophoresis. The relative sizes of outermembrance proteins of Z.mobilis appeared to be larger than those of P.aeruginosa and E.coli.
Seong-bum,Baek,Soon-jong,Kweon,Dong-soo,Park,Jong-nae,Hyun,Jong-min,Ko,Duck-yong,Suh,Young-koo,Son,Choon-woo,Lee,Hong-sik,Kim,Jong-jin,Hwang,Jung-hyun,Nam,Si-ju,Kim,Jae-seong,Choi,Jae-hwan,Seo,Yang-ki 한국육종학회 2007 한국육종학회지 Vol.39 No.1
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Gwangan' is a new six-rowed covered barley cultivar developed by the National Institute of Crop Science (NICS), R.D.A in 2004. This cultivar is developed from a cross between “Mirak” and ‘Milyang59' in 1995. An F6 selection was made at Milyang in 2001 and it was tested as ‘Suwon386' in regional adaptation yield trial (RYT) for subsequent three years. ‘Gwangan' has a winter habit (group IV) variety and compact spikes with long rough awns. It shows erect plant type with good 1000 kernel weight (34.9 g) when grown under normal cultivation in Korea. It also has strong winter hardiness and tolerance to wet-soil. The average culm length of ‘Gwangan' was 7cm shorter than that of ‘Olbori'. It is susceptible to powdery mildew. However it has strong resistance to barley yellow mosaic virus (BaYMV) except for strains of Milyang province. ‘Gwangan' yielded 12%, 10% more than ‘Olbori' in upland and paddy field of RYT, respectively. This cultivar is recommended for all of the barley cultivation area in Korea. ‘Gwangan' is a new six-rowed covered barley cultivar developed by the National Institute of Crop Science (NICS), R.D.A in 2004. This cultivar is developed from a cross between “Mirak” and ‘Milyang59' in 1995. An F6 selection was made at Milyang in 2001 and it was tested as ‘Suwon386' in regional adaptation yield trial (RYT) for subsequent three years. ‘Gwangan' has a winter habit (group IV) variety and compact spikes with long rough awns. It shows erect plant type with good 1000 kernel weight (34.9 g) when grown under normal cultivation in Korea. It also has strong winter hardiness and tolerance to wet-soil. The average culm length of ‘Gwangan' was 7cm shorter than that of ‘Olbori'. It is susceptible to powdery mildew. However it has strong resistance to barley yellow mosaic virus (BaYMV) except for strains of Milyang province. ‘Gwangan' yielded 12%, 10% more than ‘Olbori' in upland and paddy field of RYT, respectively. This cultivar is recommended for all of the barley cultivation area in Korea.
Purpose: Maspin is known as a tumor suppressor gene, but its significance has been questioned in various human cancers. The aim of this study was to investigatethe expression pattern of Maspin in human gastric adenocarcinomas and its possible correlation with clinicopathological findings. Materials and Methods: The expression of Maspin mRNA was measured by nested RT-PCR using 60 frozen adenocarcinomas of the stomach and 31 noncancerous tissues from the proximal resection margin. Immunohistochemical study for Maspin protein expression was carried out using 62 paraffin-embedded tissues, composed of both cancer and noncancerous tissues.Results: Maspin mRNA expression was detected in 80.0% (48 of 60) of the gastric adenocarcinomas, but in only 22.6% (7 of 31) of the normal gastric mucosa (p〈0.001). The positive rate of Maspin protein expression was higher in the adenocarcinomas than the normal tissues(62.9% vs. 27.4%, p〈0.05). In addition, the intestinal type of tumors showed significantly higher expression levels compared to the diffuse type of tumors (81.5% vs. 48.6%,p〈0.05). Conclusion: Our results suggest that Maspin is frequently expressed in human gastric cancers, and its expression might be associated with tumorigenesis of theintestinal type of gastric cancer