Whole-genome resequencing analysis of 20 Micro-pigs

Da‑Hye Son,Nam‑Hyun Hwang,Won‑Hyong Chung,Ha‑Seung Seong,Hyungbum Lim,Eun‑Seok Cho,Jung‑Woo Choi,Kyung‑Soo Kang,Yong‑Min Kim 한국유전학회 2020 Genes & Genomics Vol.42 No.3

Background Miniature pigs have been increasingly used as mammalian model animals for biomedical research because of their similarity to human beings in terms of their metabolic features and proportional organ sizes. However, despite their importance, there is a severe lack of genome-wide studies on miniature pigs. Objective In this study, we performed whole-genome sequencing analysis of 20 Micro-pigs obtained from Medi Kinetics to elucidate their genomic characteristics. Results Approximately 595 gigabase pairs (Gb) of sequence reads were generated to be mapped to the swine reference genome assembly (Sus scrofa 10.2); on average, the sequence reads covered 99.15% of the reference genome at an average of 9.6-fold coverage. We detected a total of 19,518,548 SNPs, of which 8.7% were found to be novel. With further annotation of all of the SNPs, we retrieved 144,507 nonsynonymous SNPs (nsSNPs); of these, 5968 were found in all 20 individuals used in this study. SIFT prediction for these SNPs identified that 812 nsSNPs in 402 genes were deleterious. Among these 402 genes, we identified some genes that could potentially affect traits of interest in Micro-pigs, such as RHEB and FRAS1. Furthermore, we performed runs of homozygosity analysis to locate potential selection signatures in the genome, detecting several loci that might be involved in phenotypic characteristics in Micro-pigs, such as MSTN, GDF5, and GDF11. Conclusion In this study, we identified numerous nsSNPs that could be used as candidate genetic markers with involvement in traits of interest. Furthermore, we detected putative selection footprints that might be associated with recent selection applied to miniature pigs.

무용전공별 척주만곡도 비교

임혜자,권혜영,서지혜,이성노 韓國舞踊敎育學會 2003 韓國舞踊敎育學會誌 Vol.14 No.1

The purpose of this study was to analyze the differences in the shape of the front, lateral, and rear sections of the spinal columns by dance majors. The results of the study are described below. First, with respect to the cervical spinal curvature of the lateral spinal column, there were a significantly differences between the general public and modern dance major person. Among other groups, however, there were no differences. Second, in the case of the thoracic spinal curvature, there were no significant differences between the general public and the people who major in Korean traditional dance. However, the measure of the thoracic curvature in modern dancers was greater than that found in ballerinas and Korean traditional dancers. Furthermore, the general public's thoracic spinal curvature was greater than the modern dancers'. Finally, there were no significant differences between modern dancers and the general public in the measure of their lumbar spinal curvature. Korean traditional dancers' lumbar spinal columns, however, were much more curved than the ballerinas'. In addition, modern dancers' lumbar spinal columns were much more curved than Korean traditional dancers'. Based on the results of the study, two recommendations can be proposed. First, training programs and practical courses for dance majors need to be improved simultaneously so that dancers can strengthen their abdominal muscles and increase their flexibility. Second, to avoid deterioration of backbone growth, the general public needs to build up their health through dancing or other exercise programs.

치매환자 조호자를 위한 교육지지프로그램의 효과성에 관한 비교연구

송 흡(Heup Song),강재명(Jae Myeong Kang),김병규(Steven B. Kim),조성진(Seong Jin Cho),윤선경(Seon kyung Yun),윤혜진(Hye jin Yoon),연병길(Byeong Kil Yeon) 대한노인정신의학회 2017 노인정신의학 Vol.21 No.1

Objective:We studied the effect of Hyu-Sig-Gong-Gan and Hye-Ah-Rim on several evaluations and compared the two programs. We then investigated factors associated with the difference between pre- and post-ratings. Methods:Thirty-six patients were randomized to Hyu-Sig-Gong-Gan (n=23) or Hye-Ah-Rim (n=13). We tested for the effect of each program on several evaluations and compared the two programs through Zarit Burden Interview (ZBI), Center for Epidemiologic Studies-Depression Scale, Dementia Attitude Scale (DAS)-intelligence, DAS-comfort and DAS-total. We then investigated whether the difference between pre- and post-rating depends on other factors. Results:We observed significant positive effect of Hyu-Sig-Gong-Gan on DAS-comfort (t=1.843, p=0.039) and significant positive effect of Hye-Ah-Rim on ZBI (t=-2.327, p=0.019), DAS-comfort (t=2.241, p=0.022), and EuroQol-5 Dimensions-Visual Analogue Scale (t=2.537, p=0.013). For comparing the two programs, we found a lack of evidence for the difference in any of the five commonly observed evaluations. In the secondary analysis, we observed a strong trend that the two programs were particularly effective for caregivers with negative pre-ratings. Conclusion:The two programs showed positive effects on the various evaluations, but some evaluations could not reach statistical significance. The programs appear to be particularly helpful for those who are in need before the program participation.

개의 콜레스테롤 담석 증례

전혜영,박성환,한재익,이숙진,김근형,정동우,나기정 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

본문참조

성장기 어린이에서 상악 중절치 조기 상실 후 치조골 소실

나혜진,송제선,이제호,최형준,김성오,손흥규,최병재 大韓小兒齒科學會 2012 大韓小兒齒科學會誌 Vol.39 No.1

어린이는 8-10세 경에 외상을 자주 받으며 구강 내에서는 상악 절치부가 호발하는 부위이다. 외상으로 인하여 상악 절치가 상실되면 어린이에게 합병증을 야기할 수 있으므로 가능한 한 상악 절치를 유지해야 하지만 조기 상실되는 경우가 있다. 상악 절치의 조기 상실로 인한 합병증으로는 심미적 문제나 치조골의 수직적 높이와 수평적 너비의 감소, 인접치의 경사, 악궁 장 경 감소 등이 있다. 그 중에서 치조골이 소실되면 보철적 수복 시에 기능성과 안정성, 심미적인 면에 영향을 준다. 이 증례는 외상으로 인하여 상악 중절치가 조기 상실된 9세 여아와 6세 남아에서 각각 5년 5개월, 3년 7개월 후의 치조골 소실 정도를 cone beam computed tomography와 진단 모형 상에서 관찰하였기에 보고하는 바이다. The anterior maxillary incisor is the most traumatized region in the mouth and trauma is frequent between the ages of 8-10. Traumatic loss of teeth, can lead to many complications in children. Thus, as possible to keep traumatic teeth, but if you need extractions There may be. Complications occur and early tooth loss is frequent. Complications of early loss of central incisors are esthetic compromise, loss of vertical and horizontal width, height, contour of alveolar bone, tilting of adjacent teeth, arch length loss. Alveolar bone loss may affect normal function and stability, and results in esthetic problem for future prosthesis restoration. The 9-year-old girl and 6-year-old boy got early loss of upper central incisor. The amount of alveolar bone resorption was measured using cone beam computed tomograph and cast analysis.

운동신경원성 질환을 동반한 전측두엽 치매 1례

최성혜,나덕렬,김병준,이정일,서연림,김상은 대한신경과학회 1998 대한신경과학회지 Vol.16 No.4

[논문] trn L-trn F에 의한 한국 족도리풀속 식물 종 분류

성혜경,허만규,허홍욱 釜山大學校 師範大學 科學敎育硏究所 2011 科學敎育硏究報 Vol.38 No.-

Asarum consists low-growing herbs and is a genus in the Aristo-lochiaceae family with species found in the north temperate zones with most species in Asia. Any species with a highly structured variation pattern must show different distributions of phenotypic characteristics at different locations. I analysed intra- and interspecific phylogenic relationships within genus Asarum in Korea. I evaluated a representative sample of the fourteen taxa (eight Korean taxa and six obtained taxa from GenBank) with the trn L intron and the trn L - trn F spacer region of the chloroplast genome to estimate genetic relationships within genus Asarum. No variable positions were found within the trn L 5` exon and trn L 3` exon. Variable positions within the intron region were mostly distributed outside the structural elements required for intron processing, i.e., not within the first 140 bp at the 5` end and not within the 90 bp at the 3` end of the intron region. Within the spacer region, variable positions were evenly distributed. Alignment of the DNA sequences required adding numerous gaps. Sequence variation within the Asarum is mostly due to nucleotide substitutions, although several small indels and 20 inserts can be found. Another source of sequence divergence is length variation due to stretches of short repeats that occur at the intergenic spacer region ITS2 in all the Asarum. A + T content for eight Korean species of genus Asarum ranged between 64.3% and 64.6%. The base furtherance did not showed the significant difference to the by a total taxon. These values are similar to those (64.5%~67.1%) for the angiosperm alignments of the total trn L and trn F region. The main phylogenetic analysis revealed two distinct clades. The first clade includes ten species (eight Korean species, A. nipponicum, and A. tamaensis). The second clade includes four species (A. canadense , A. cautatum, A. chingchengense , and A. yakusimense). However, some internal nodes are poorly supported. Within Korean Asarum, four species were unsolved portions.

고양이에서 영양학적인 원인에 의해 발생한 속발성 부갑상선기능항진증 1례

이혜원,박성준,김명철,정성목 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2009 動物醫科學硏究誌 Vol.16 No.1

A 10 month-old Korean domestic shorthaired cat(male, 1.4kg)was admitted to the Veterinary Medical Teaching Hospital of Chungnam National University with the history of astasia the onest of which was 3 month ago and all-meat diets. Physical examination revealed thin, growth retarded and lying in lateral recumbency. Radiograph showed abnormally radiolucent bones, thin cortices, kyphosis and lordosis of the spine and multiple fractures of the femurs. Result of serum biochemical test revealed mild hypocarcemia. Serum parathormone and 1, 25(OH)2-vitamin D3 levels were markedly elevated, whereas 25(OH)-vitamin D3 level was in normal range. Nutritional secondary hyperparathyroidism was diagnosed by history taking, physical examination, laboratory examination and radiography. Calcium gluconate with balanced commercial cat food was given. On 24 days later, improvements were found in clinical signs and radiographs.

개에서 발생한 만성 단독 비장 염전의 진단 및 치료 1례

이혜원,최호정,김명철,정성목 忠南大學校 獸醫科大學 附設 動物醫科學硏究所 2009 動物醫科學硏究誌 Vol.16 No.1

A 5-year-old German Shepherd dog(intact male, 27kg) was admitted to the Veterinary Medical Teaching Hospital of Chungnam National University with the history of anorexia and vomiting. On physical examination, he was depressed and had abdominal distention. A complete blood count indicated leukocytosis and anemia. Radiographic findings included a large soft tissue mass in the middle to caudal abdomen and loss of serosal detail. Ultrasonographs showed splenomegaly with mixed ethoic parenchyma, hyperechoic area around the splenic hilus, and loss of venous flow. Computed tomography revealed enlarged spleen with partially enhanced parenchyma. Chronic isolated splenic torsion was diagnosed and splenectomy was performed. Histopathologic examination revealed chronic passive congestion of the spleen. He was recovered normally after surgery without any complications.

상악 절치부에 매복된 다수 과잉치의 외과적 발거

홍은혜,김성오,이제호,최형준,송흥규,최병재 大韓小兒齒科學會 2008 大韓小兒齒科學會誌 Vol.35 No.2

과잉치란 정상에 비해 많은 수의 치아를 일컫는 것으로 유치열기와 혼합치열기에 발생하며,여러 가지 임상적 문제를 일으킬 수 있다. 특히 상악 전치부의 과잉치는 상악 전치의 맹출 장애 및 전위,정중부의 치간 이개,치근 흡수,함치성 낭종 형성 등의 문제를 유발할 수 있으므로 조기에 진단하고,과잉치의 위치 및 수,형태에 따라 적절한 치료를 시행하는 것이 중요하다. 이 증례는 상악 전치부에 네 개의 과잉치가 영구치의 맹출을 방해하고 있는 경우로,발거 시 합병증을 최소화하기 위하여 두 단계로 나누어 발거하였다. 컴퓨터 단층 촬영에서 네 개의 과잉치가 주위의 영구치 맹출을 방해하고 있었고,그 중 두 개의 과잉치는 절치 치배에 매우 근접해 있었다. 네 개의 과잉치를 동시에 제거할 경우 영구 치배에 손상을 줄 위험이 있으므로 두 개의 역위 매복된 원추형의 과잉치만 먼저 발거하였다. 남은 두 개의 과잉치는 위치가 변화되기를 기다린 후 두 번째 발거 수술을 시행하여,주위 절치 치배에 손상을 가하지 않고 치조골 삭제를 적게 하여 합병증 발생을 줄일 수 있었다. Supernumerary teeth are characterized by an excess number of teeth, which can be responsible for a variety of irregularities in the primary and transitional dentition, Supernumerary teeth, especially in the maxillary anterior region, may prevent the eruption of adjacent permanent teeth and cause their ectopic eruption, diastema, root resorption, or formation of dentigerous cyst. Therefore, early diagnosis of supernumerary teeth is important for prevention of such complications, and adequate treatment should be given according to their location, number, and morphologic features. In this case. four supernumerary teeth in the maxillary anterior region were disturbing the eruption of adjacent permanent incisors. Two of them were located in proximity to the central incisor tooth germs that their immediate removal may injure the permanent tooth germs, In order to minimize such complications, surgical extraction of the four supernumerary teeth was performed in two stages. At first, only two inverted conical supernumerary teeth were extracted. The other two tuberculous supernumerary teeth, close to the permanent tooth germs, were extracted later after their natural dislocation, In that way, we could minimize affects on the neighboring permanent tooth germs and also the amount of alveolar bone removed during surgery.