A dual inhibitor of PIP5K1C and PIKfyve prevents SARS-CoV-2 entry into cells

Seo Yuri,Jang Yejin,Lee Seon-gyeong,Rhlee Joon Ho,Kong Sukyeong,Vo Thi Tuyet Hanh,Kim Myung hun,Lee Myoung Kyu,Kim Byungil,Hong Sung You,Kim Meehyein,Lee Joo-Yong,Myung Kyungjae 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-

The SARS-CoV-2 pandemic has had an unprecedented impact on global public health and the economy. Although vaccines and antivirals have provided effective protection and treatment, the development of new small molecule-based antiviral candidates is imperative to improve clinical outcomes against SARS-CoV-2. In this study, we identified UNI418, a dual PIKfyve and PIP5K1C inhibitor, as a new chemical agent that inhibits SARS-CoV-2 entry into host cells. UNI418 inhibited the proteolytic activation of cathepsins, which is regulated by PIKfyve, resulting in the inhibition of cathepsin L-dependent proteolytic cleavage of the SARS-CoV-2 spike protein into its mature form, a critical step for viral endosomal escape. We also demonstrated that UNI418 prevented ACE2-mediated endocytosis of the virus via PIP5K1C inhibition. Our results identified PIKfyve and PIP5K1C as potential antiviral targets and UNI418 as a putative therapeutic compound against SARS-CoV-2.

Laminated Graphene Films for Flexible Transparent Thin Film Encapsulation

Seo, Hong-Kyu,Park, Min-Ho,Kim, Young-Hoon,Kwon, Sung-Joo,Jeong, Su-Hun,Lee, Tae-Woo American Chemical Society 2016 ACS APPLIED MATERIALS & INTERFACES Vol.8 No.23

<P>We introduce a simple, inexpensive, and large area flexible transparent lamination encapsulation method that uses graphene films with polydimethylsiloxane (PDMS) buffer on polyethylene terephthalate (PET) substrate. The number Of stacked graphene layers (n(G)) was increased from 2 to 6, and 6-layered graphene-encapsulation showed high impermeability to moisture and air. The graphene-encapsulated polymer light emitting diodes (PLEDs) had stable operating characteristics; and the operational lifetime of encapsulated PLEDs increased as nG increased. Calcium oxidation test data confirmed the improved impermeability of graphene-encapsulation with increased nG. As a practical application, we demonstrated large-area flexible organic light emitting diodes (FOLEDs) and transparent FOLEDs that were encapsulated by our polymer/graphene encapsillant.</P>

Korean Association for Clinical Oncology : Slide Session ; OP4-2 : Others ; Chemotherapy for the Patients with Metastatic Primary Adenocarcinoma of Bladder

( Sung Yong Oh ),( Suee Lee ),( Sung Hyun Kim ),( Ji Hyun Lee ),( Hyo Jin Kim ),( Jung Hun Kang ),( Soon Il Lee ),( Young Mi Seo ),( Young Jin Choi ),( Min Jae Park ),( Moon Jin Kim ),( Ho Yeong Lim ) 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

Background: By defi nition, primary adenocarcinoma of the bladder (PAB) is a malignant neoplasm derived from urothelium of the bladder showing histologically pure glandular differentiation.Because of its rarity, role of chemotherapy for metastatic PAB is still questionable. Therefore, we performed a retrospective analysis of the clinical features and chemotherapy outcomes of metastatic PAB. Methods: Eligible patients for this retrospective analysis were initially diagnosed with adenocarcinoma and presented with a clinically no other primary site of origin. Unifi ed case report forms were provided to participating institutions. Results: We retrospectively reviewed 27 patients who treated with chemotherapy as metastatic PAB at nine Korean medical institutions from 2004 to 2014.The median age of patients was 58 years (range, 26 to 78 years) and 51.9% of the patients were female. Fourteen patients were urachal adenocarcinoma. Of 25 symptomatic patients, 19 experienced gross hematuria. Ten patients had two or more metastatic sites of the lungs (48.1%), peritoneum (33.3%), and ovary (22.2%), as the most common sites. Eleven patients were treated with 5-FU based chemotherapy, 4 were gemcitabine based, 3 were taxane and adriamycin based, and others. 13 of them achieved CR (14.8%) or PR (33.3%). Median PFS and OS for all patients were 10.6 months (95% confi dence interval [CI], 9.6 to 11.6 months) and 23.5 months (95% CI, 13.6 to 33.4 months), respectively. In the prognostic factor analysis, the cases of urachal adenocarcinoma had worse tendency in PFS and OS (p=0.058 and P=0.113, respectively). Conclusions: Metastatic PAC is a highly aggressive form of bladder cancer. Despite most of chemotherapy, PFS and OS were short, however, there were some long-term survivors, therefore, additional research on the predictive markers of several clinical, pathological differences and their treatments will be needed.

A Study on the Desulfurization Efficiency of Limestone Sludge with Various Admixtures

Seo, Sung Kwan,Chu, Yong Sik,Shim, Kwang Bo,Lee, Jong Kyu,Song, Hun The Korean Ceramic Society 2015 한국세라믹학회지 Vol.52 No.6

The flue gas desulfurization (FGD) process is one of the most effective methods to reduce the amount of $SO_2$ gas (up to 90%) generated by the use of fossil fuel. Limestone is usually used as a desulfurizing agent in the wet-type FGD process; however, the limestone reserves of domestic mines have become exhausted. In this study, limestone sludge produced from the steel works process is used as a desulfurizing agent. Seven different types of additives are also used to improve the efficiency of the desulfurization process. As a result, alkaline additive is identified as the least effective additive, while certain types of organic acids show higher efficiency. It is also observed that the amount of FGD gypsum, which is a by-product of the FGD process, increases with the used of some of those additives.

Safety and efficacy of nilotinib in adult patients with chronic myeloid leukemia: a post-marketing surveillance study in Korea

Seo-Yeon Ahn,Sang Kyun Son,Gyu Hyung Lee,Inho Kim,June-Won Cheong,Won Sik Lee,Byung Soo Kim,Deog-Yeon Jo,Chul Won Jung,Chu Myoung Seong,Jae Hoon Lee,Young Jin Yuh,Min Kyoung Kim,Hun-Mo Ryoo,Moo-Rim Pa 대한혈액학회 2022 Blood Research Vol.57 No.2

Background Nilotinib is a tyrosine kinase inhibitor approved by the Ministry of Food and Drug Safety for frontline and 2nd line treatment of Philadelphia chromosome-positive chronic myeloid leukemia (Ph+ CML). This study aimed to confirm the safety and efficacy of nilotinib in routine clinical practice within South Korea. Methods An open-label, multicenter, single-arm, 12-week observational post-marketing surveillance (PMS) study was conducted on 669 Korean adult patients with Ph+ CML from December 24, 2010, to December 23, 2016. The patients received nilotinib treatment in routine clinical practice settings. Safety was evaluated by all types of adverse events (AEs) during the study period, and efficacy was evaluated by the complete hematological response (CHR) and cytogenetic response. Results During the study period, AEs occurred in 61.3% (410 patients, 973 events), adverse drug reactions (ADRs) in 40.5% (271/669 patients, 559 events), serious AEs in 4.5% (30 patients, 37 events), and serious ADRs in 0.7% (5 patients, 8 events). Furthermore, unexpected AEs occurred at a rate of 6.9% (46 patients, 55 events) and unexpected ADRs at 1.2% (8 patients, 8 events). As for the efficacy results, CHR was achieved in 89.5% (442/494 patients), and minor cytogenetic response or major cytogenetic response was achieved in 85.8% (139/162 patients). Conclusion This PMS study shows consistent results in terms of safety and efficacy compared with previous studies. Nilotinib was well tolerated and efficacious in adult Korean patients with Ph+ CML in routine clinical practice settings.

Development of an Efficient Brown Macroalgae Bioprocess Using a Novel Microbial Host; Vibrio sp. dhg

Sung Hwa WOO,Hyun Gyu LIM,Dong Hun KWAK,Sungwoo PARK,Snag Woo SEO,Gyoo Yeol JUNG 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.4

Poster Session:PS 1170 ; Cardiology : Prevalence of Gastrointestinal Diseases and Status of Treatment in Noncardiac Chest Pain Patients

( Sung Hun Park ),( Chang Yu Park ),( Ji No Na ),( Cheol Ung Choi ),( Hongeuy Lim ),( Jin Won Kim ),( Eung Joo Kim ),( Seung Woon Rha ),( Hong Seog Seo ),( Dong Joo Oh ) 대한내과학회 2014 대한내과학회 추계학술발표논문집 Vol.2014 No.1

Background: We evaluated the prevalence of gastroesophageal refi ux diseases (GERD) in noncardiac chest pain (NCCP) patients, risk factors for GERD, and status of prescriptions for GERD in Korean population. Methods: This was a retrospective non-interventional observational nation-wide multicenter (45 centers) study. Patients who had a normal coronary angiogram (CAG) and upper gastroendoscopy within 2 years after CAG were enrolled. The prevalence of GERD among them was examined. Other gastrointestinal diseases including peptic ulcer diseases or gastritis were also examined. We compared the risk factors for GERD between the GERD group and non-GERD group. The ratio of patients who had a medications for gastrointestinal diseases (antacids or proton-pump inhibitor) was also examined. Results: Total of nine hundred four patients were enrolled. The prevalence of GERD among NCCP patients was 436(48. 2%) of 904 patients. Peptic ulcer disease were present in 154 patients, 17. 0%. The prevalence of gastritis was 659(72. 9%) of 904 patients. There was no difference in risk factors for GERD between GERD group and non- GERD group. Medications for GERD and other gastrointestinal diseases were prescribed in 742 patients, 82. 1% of all the NCCP patients. Conclusions: GERD were common (42. 8%) in Korean NCCP patients and most of them (82. 1%) were prescribed for GI medications in Korea. But we could not fi nd any differences in risk factors between GERD and non-GERD groups.

Osteological Development of the Larvae and Juvenile of Bullhead torrent catfish, Liobagrus obesus

Seo, Won-Il,Park, Jae-Min,Lee, Sung-Hun,Yoon, Seong Min,Hwang, Seon-Yeong,Han, Kyeong-Ho The Korean Society of Developmental Biology 2018 발생과 생식 Vol.22 No.1

This study was conducted to investigate the skeletal development of bullhead torrent catfish, Liobagrus obesus larvae and to utilize them as basic data for the taxonomic study of Liobagrus larvae. Skeletal development was observed by being divided into cranium, visceral skeleton, shoulder girdle bone, pelvic girdle bone and vertebra. On the first day after hatching, the pre-larvae had an average total length of 7.92 mm, and a line-shaped parasphenoid ossified in the cranium. In the jaw bone, the dentary supporting the lower jaw and the maxillary supporting the upper jaw were ossified. In the anterior abdominal vertebrae of the vertebra, seven centrums began to ossify and five neural spines ossified simultaneously. On the 3 day after hatching, pre-larvae had an average total length of 8.95 mm, and the prefrontal ossified in cranium. The number of abdominal vertebrae was increased to 14, and three parapophysis developed from the front side. On the 24th day after hatching, post-larvae had an average total length of 15.2 mm and the epural bone ossified in coccyx. The parhypural bone was ossified, and ossification of coccyx and pelvic girdle bone was completed. On the 30th day after hatching, the average total length of the juvenile was 17.8 mm, and the ossification of cranium and visceral skeleton was all completed while the preorbital and three suborbitals were ossified in the orbital region of the cranium.

Single-crystal Structure of Fully Dehydrated and Largely NH₄⁺-exchanged Zeolite Y (FAU, Si/Al = 1.70), │(NH₄)₆₀Na₁₁│[Si₁₂₁Al₇₁O₃₈₄]-FAU

Seo, Sung-Man,Kim, Ghyung-Hwa,Kim, Young-Hun,Wang, Lian-Zhou,Lu, Gao-Qing,Lim, Woo-Taik Korean Chemical Society 2009 Bulletin of the Korean Chemical Society Vol.30 No.3

The single-crystal structure of largely ammonium-exchanged zeolite Y dehydrated at room temperature (293 K) and 1 ${\times}\;10^{-6}$ Torr. has been determined using synchrotron X-radiation in the cubic space group $Fd\overline{3}m\;(a=24.9639(2)\AA)$ at 294 K. The structure was refined to the final error index $R_1$ = 0.0429 with 926 reflections where $F_o>4\sigma(F_o)$; the composition (best integers) was identified as |$(NH_4)_{60}Na_{11}$|[$Si_{121}Al_{71}O_{384}$]-FAU. The 11 $Na^{+}$ ions per unit cell were found at three different crystallographic sites and 60 ${NH_4}^{+}$ ions were distributed over three sites. The 3 $Na^{+}$ ions were located at site I, the center of the hexagonal prism ($Na-O\;=\;2.842(5)\;\AA\;and\;O-Na-O\;=\;85.98(12)^{\circ}$). The 4 $Na^{+}$ and 22 ${NH_4}^{+}$ ions were found at site I' in the sodalite cavity opposite the double 6-rings, respectively ($Na-O\;=\;2.53(13)\;\AA,\;O-Na-O\;=\;99.9(7)^{\circ},\;N-O\;=\;2.762(11)\;\AA,\;and\;O-N-O =\;89.1(5)^{\circ}$). About 4 $Na^{+}$ ions occupied site II ($(Na-O\;=\;2.40(4)\;\AA\;and\;O-Na-O\;=\;108.9(3)^{\circ}$) and 29 ${NH_4}^{+}$ ions occupy site II ($N-O\;=\;2.824(9)\;\AA\;and\;O-N-O\;=\;87.3(3)^{\circ}$) opposite to the single 6-rings in the supercage. The remaining 9 ${NH_4}^{+}$ ions were distributed over site III' ($N-O\;=\;2.55(3),\;2.725(13)\;\AA\;and\;O-N-O\;=\;94.1(13),\;62.16(15),\;155.7(14)^{\circ}$).

Carotid artery intima-media thickness in Behcet`s disease patients without significant cardiovascular involvement

( Seo Na Hong ),( Jong Chun Park ),( Nam Sik Yoon ),( Sang Rok Lee ),( Kye Hun Kim ),( Young Joon Hong ),( Hyung Wook Park ),( Ju Han Kim ),( Young Keun Ahn ),( Myung Ho Jeong ),( Jeong Gwan Cho ),( J 대한내과학회 2008 The Korean Journal of Internal Medicine Vol.23 No.2

Background/Aims: Behcet`s disease (BD) is a systemic disorder associated with a characteristic vasculitis that can involve both veins and arteries of all sizes. Endothelial activation or injury is a characteristic feature of BD. Endothelial dysfunction is widely regarded as being the initial lesion in the development of atherosclerosis. The carotid artery intima-media thickness (IMT) is a widely accepted marker of subclinical atherosclerosis. We aimed to determine the carotid IMT in BD patients with using high-resolution B-mode Doppler ultrasonography. Methods: We studied 40 patients (24 males, mean age: 39.1±8.5 years) who were diagnosed by the international diagnostic criteria of Behcet`s disease and 20 healthy controls (13 males, mean age: 40.2±5.1 years), and the two groups were matched by age and gender. No subject in either group had a history of atherosclerosis or its complications. The clinical data, including the age of onset, the duration of disease, a history of medication, the activity score and the laboratory data were analyzed. Results: The carotid IMT in the BD group was significantly higher than that in the control group (0.71±0.22 mm vs. 0.59±0.09 mm, respectively, p<0.01). Cardiac and major vessel involvements were not identified in the BD group. However, minor vascular involvements were documented in 2 patients with deep vein thrombosis, in 4 patients with superficial thrombophlebitis and in 2 patients with pseudoaneurysm. The carotid IMT in the patients with posterior uveitis or retinal vasculitis was higher than that of the patients without these findings (0.85±0.21 mm vs. 0.64±0.10 mm, respectively, p=0.007), but there was no difference of the IMT according to minor vascular involvement. Conclusions: Despite that there was no significant cardiovascular involvement in the BD patients, the carotid IMT was significantly higher in the BD patients as compared with the healthy controls.