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Dimethylnitrosamine 유발 급성 간 손상 흰쥐에서 ^(99m)Tc-Lactosylated Serum Albumin을 이용한 간 기능의 평가
정신영,이재태,서명랑,유정아,배진호,안병철,황재석,정재민,하정희,이규보 대한핵의학회 2003 핵의학 분자영상 Vol.37 No.6
목적: ^(99m)Tc-lactosylated serum albumin (^(99m)Tc-LSA)은 간세포에 특이적으로 결합하는 간수용체 영상용 방사성의약품으로 새로이 합성되었다. 간섬유화를 유발하는 dimethylnitrosamine (DMN)을 투여한 간 손상 휜쥐 모델에서 ^(99m)Tc-LSa의 역동학적인 간섭취를 조사하고 간효소치의 변화와 조직학적 소견을 비교하여, LSA의 간섭취가 간기능의 변화를 반영하는지를 연구하였다. 대상 및 방법: SD계 흰쥐에 DMN를 27 mg/kg으로 복강 내 주사하여 급성 간손상을 유도하고 대조군과 비교하였다. DMN을 주사한 흰쥐를 3일(DMN-3), 8일(DMN-8), 21일(DMN-21)에 ^(99m)Tc=LSA (1,665 mg/kg) 29 MBq를 정맥 주사하여, 30분 동안 동적 영상을 획득하고 간과 신장부위에 관심영역을 설정하여 간과 심장부위의 시간방사능 곡선을 얻었다. 간기능 평가를 위해 시간방사능 곡선을 이용하여 간섭취지수와 혈중제거지수를 구하였고 곡선 최적화를 시행하였다. DMN 투여군과 대조군의 간효소치의 변화와 간조직의 광학현미경 소견을 비교하였다. 결과: 대조군에서는 ^(99m)Tc-LSA가 빠르게 간에 섭취되고 혈중에서 제거되었으나 DMN을 처리한 군에서는 간섭취가 낮았다. 간섭취지수의 비교에서 대조군에 비해 DMN 처리군에서 유의하게 간섭취지수가 낮았다(DMN-3: 0.842, DMN-8: 0.898, DMN-21: 0.91, 대조군: 0.96, p<0.05). 혈중제거지수의 비교에서도 대조군에 비해 DMN 처리군에서 혈중제거지수가 유의하게 높았다(DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, 대조군: 0.473, p<0.05). 비선형 회귀분석에서 R_(2) 값은 0.9이상으로 좋은 일치를 보였고, 대조군에ㅓ K값이 DMN처리군에 비해 크고(DMN-3: 0.28, DMN-8: 0.41, DMN-21: 0.46, 대조군: 0.97, p<0.05), T_(1/2)값은 작았다(DMN-3: 2.5, DMN-8: 1.7, DMN-21: 1.5, 대조군: 0.7, p<0.05). 간효소치의 변화는 DMN-3군에서는 대조군에 비해 상승하였으나 DMN-8, DMN-21군에서는 간효소치의 상승이 관찰되지 않았다. 간조직 소견의 경우 DMN-3군에서 중심정맥 주위에 괴사가 관찰되었으나 DMN-8군, DMN-21군에서는 미약한 정도의 염증세포 침윤만이 관찰되었다. 결론: ^(99m)Tc-LSA 간신티그래피의 간섭취 정도는 간손상과 반비례하였으며 간섭취의 변화는 조직학적 손상이 심한 정도와 간손상후 회복되는 과정을 반영하여 주었다. ^(99m)Tc-LSA 간신티그래피가 간손상을 평가하고 간손상후 회복되는 과정을 추적하는 간수용체 영상용 방사성 의약품으로 사용될 수 있을 것으로 생각된다. Objects: ^(99m)Tc-lactosylated human serum albumin(LSA) is a newly synthesized radiopharmaceutical that binds to asialoglycoprotein receptors, which are specifically presented on the hepatocyte membrane. Hepatic uptake and blood clearance of LSA were evaluated in rat with acute hepatic injury induced by dimethylnitrosamine(DMN) and results were compared with corresponding findings of liver enzyme profile and these of histologic changes. Materials and Methods: DMN (27 mg/kg) was injected intraperitoneally in Sprague-Dawley rat to induce acute hepatic injury. At 3(DMN-3), 8(DMN-8), and 21(DMN-21) days after injection of DMN, LSA injected intravenously, and dynamic images of the liver and heart were recorded for 30 minutes. Time-activity curves of the heart and liver were generated from regions of interest drawn over liver and heart area. Degree of hepatic uptake and blood clearance of LSA were evaluated with visual interpretation and semiquantitative analysis using parameters (receptor index : LHL3 and index of blood clearance : HH3), analysis of time-activity curve was also performed with curve fitting using Prism program. Results: Visual assessment of LSA images revealed decreased hepatic uptake in DMN treated rat, compared to control group. In semiquantitative analysis, LHL3 was significantly lower in DMN treated rat group than control rat group (DMN-3:0.842, DMN-8: 0.898, DMN-21: 0.91, Control: 0.96, p<0.05), whereas HH3 was significantly higher than control rat group (DMN-3: 0.731, DMN-8: 0.654, DMN-21: 0.604, Control: 0.473, p<0.05). AST and ALT were significantly higher in DMN-3 group than those of control group. Centrilobular necrosis and infiltration of inflammatory cells were most prominent in DMN-3 group, and were decreased over time. Conclusion: The degree of hepatic uptake of LSA was inversely correlated with liver transaminase and degree of histologic liver injury in rat with acute hepatic injury.
서진기,정상훈,박동원 동아대학교 공과대학부설 생산기술연구소 1996 生産技術硏究所硏究論文集 Vol.1 No.2
The experimental binodal curves and tie lines were determined for solvent-5% KF salt solution-1 propanol systems at 25℃, and those tie line data were used to test thermodynamic consistency. From experimental data of tie line, the parameter estimated using NRTL and the caculated values fo tie line were predicted. The effect of KF salt on liquid-liquid equilibria was also studied.
서진욱,정재훈,최용의,이학성,신차균 중앙대학교 유전공학연구소 2003 遺傳工學硏究論集 Vol.16 No.1
The enzyme, squalene synthase, represents a branch point in the isoprenoid pathway capable of diverting carbon flow specifically to the biosynthesis of phytosterol and Triterpenoid. Transgenic Eleutherococcus senticosus plants were prepared by introducing the genes for squalene synthase derived from Panax ginseng (PgSS1), hygromycin phosphotransferase (HPT) and green fluorescent protein (GFP) through Agrobacterium-mediated transformation. Establishment of transgenic plantlets were confirmed by the presence of PgSS1 and HPT bands in the genomic DNA preparation using a PCR method and a Southern blotting. In addition, expression of the introduced GFP DNA was confirmed by observing green fluorescence of GFP from the embroyos. In the in vitro analysis of the PgSS1 enzymatic activities the transgenic plants showed to have 1.5 to 3 times higher than wild type plant, indicating that the PgSS1 genes was well over-expressed in the transgenic plants. Especially the PgSS1 acivities of embroyogenic cell was 120 times higher than those of plantlet.
구간 분할 제어를 이용한 형상기억합금 와이어의 온도제어에 관한 연구
정상화,김주환,김태호,서호진,박진완 朝鮮大學校 機械技術硏究所 2007 機械技術硏究 Vol.10 No.1
The key technology of robot is the design of the actuator. Particularly breakthroughs of power-to-weight ratio or energy-density III actuator technology have significant impacts upon the design and the control of robotic systems. To realize the anthropomorphic motion, artificial muscles such as shape memory alloy(SMA) and electro active polymer(EAP) are used. These actuators have the higher energy density than the electromechanical actuators such as the electric motor. The EAP has the property of good elasticity, but it is difficult to get the required power. The SMA has the good power density and simple structure, but the control scheme of the actuator is difficult. In this paper, temperature control of SMA wire using segment control is studied. The SMA wire is segmented by thermo-electric module(TEM). To supply constant currents in TEMs, MOSFET is used. Hysteresis in one segment of SMA wire is evaluated. The force and displacement of SMA wire are measured according to the number of segment. The program to control the temperature of TEM is developed by using LabVIEW software.
효소고정화막과 반투막성질의 PVA적층막 제조 및 산소센서에서의 특성
서종원,김태진,정용섭,윤정원 경북대학교 센서기술연구소 1998 센서技術學術大會論文集 Vol.9 No.1
The oxygen electrode of biosensor needs enzyme immobilized membrane and dialysis membrane for measurement of oxygen concentration, which in solution after enzyme reaction with its substrate. The one-layer PVA laminated CTA/PCL membrane was developed for oxygen electrode of biosensor. Enzymes have immobilized on cellulose triacetate/polycarprolactone membrane by CDI method, and laminated with polyvinyl alcohol, aldehyde and acid. This membrane immobilized with glucose oxidase, ascorbate oxidase, pyruvate oxidase and alcohol oxidase by CDI mehod, and PVA laminated CTA/PCL membrane have been tested with various concentration of substrates of enzymes by use of YSI oxygen sensor. Under 5-10mmol substrates become 0.37-0.83μA(r=0.995) currents, and about 56% of glucose oxidase activity was remained after 8 weeks, others were remained very low. SEM shows smooth surface and tightly attached PVA on enzyme immobilized CTA/PCL membrane.