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Rosentritt, Martin,Schumann, Frederik,Krifka, Stephanie,Preis, Verena The Korean Academy of Prosthodonitics 2020 The Journal of Advanced Prosthodontics Vol.12 No.1
PURPOSE. To investigate the influence of crown material (lithium-disilicate, 3Y-TZP zirconia) and abutment type (rigid implant, resin tooth with artificial periodontium) on wear performance of their antagonist teeth and adjacent teeth. MATERIALS AND METHODS. A mandibular left first molar (#36) with adjacent human teeth (mandibular left second premolar: #35, mandibular left second molar: #37) and antagonistic human teeth (maxillary left second premolar: #25, maxillary left first molar: #26, maxillary left second molar: #27) was prepared simulating a section of the jaw. Samples were made with extracted human molars (Reference), crowned implants (Implant), or crowned resin tooth analogues (Tooth). Crowns (tooth #36; n = 16/material) were milled from lithium-disilicate (Li, IPS e.max CAD) or 3Y-TZP zirconia (Zr, IPS e.max ZirCAD, both Ivoclar Vivadent). Thermal cycling and mechanical loading (TCML) in the chewing simulator were applied simulating 15 years of clinical service. Wear traces were analyzed (frequency [n], depth [㎛]) and evaluated using scanning electron pictures. Wear results were compared by one-way-ANOVA and post-hoc-Bonferroni (α = 0.05). RESULTS. After TCML, no visible wear traces were found on Zr. Li showed more wear traces (n = 30-31) than the reference (n = 21). Antagonistic teeth #26 showed more wear traces in contact to both ceramics (n = 27-29) than to the reference (n = 21). Strong wear traces (> 350 ㎛) on antagonists and their adjacent teeth were found only in crowned groups. Abutment type influenced number and depth of wear facets on the antagonistic and adjacent teeth. CONCLUSION. The clinically relevant model with human antagonistic and adjacent teeth allowed for a limited comparison of the wear situation. The total number of wear traces and strong wear on crowns, antagonistic and adjacent teeth were influenced by crown material.
Optimization of the Secretion Pathway for Heterologous Proteins in Bacillus subtilis
박성훈,Wolfgang Schumann 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4
Secretion of homologous proteins in large amounts has been accomplished for many proteins, but no efficient secretion system has been described so far which can be generally applied for heterologous proteins. The objective of this review article is to compare the three major secretion pathways in E. coli and in B. subtilis and review the stages of conversion of the secreted proteins from the unfolded polypeptide chains into the correctly folded and fully active protein. Furthermore, bottlenecks in the production of heterologous proteins and the ways to resolve them are briefly discussed.
( James F. Hokanson ),( Allison Schumann ),( Casey Austin ) 한국체육학회 2020 International journal of human movement science Vol.14 No.1
Lower body positive pressure treadmills (LBPPT) are beneficial for rehabilitation and exercise training. Running on a LBPP treadmill (AlterG<sup>TM</sup>) involves sealing the lower half of the body in a high air pressure flexible sealed chamber to simulate exercise at lower body weight. The effects of the sealed chamber on treadmill and tympanic temperature during exercise have yet to be investigated. The purpose of the study was to measure treadmill and tympanic temperatures while participants were running on a normal treadmill and compare temperatures to running on a LBPP treadmill. Fifteen well-trained runners completed three running trials on a LBPP treadmill at 60, 75, and 85% of body weight (BW). A 100% BW trial was run on a normal treadmill. Participants ran at three steady state speeds (2.9, 3.4, and 3.8 m·s<sup>-1</sup>) for four minutes each. Room air, tympanic, and LBPPT chamber temperatures (°C) were recorded. The main finding was that treadmill temperature was significantly greater among the three BW conditions on the LBPPT compared to normal treadmill (average of all LBPPT, 30.7 ± 2.3°C, normal treadmill, 22.5 ± 2.1°C). Tympanic temperatures were not different between all BW conditions. Runners may use LBPPT for rehabilitation or low-impact training yet should be aware of the increase in LBPP chamber temperature.