Effect of Conjoint Application of Sewage Sludge and Fertilizers on Trace Metals Accumulation in Plant and Soil under Rice Cultivation

Satish Kumar Singh,Ashish Latare 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Alkaline Peroxide Pretreatment of Waste Lignocellulosic Sawdust for Total Reducing Sugars

Satish Kumar Singh,Sweety Verma,Ishan Gulati,Suman Gahlyan,Ankur Gaur,Sanjeev Maken The Korean Institute of Chemical Engineers 2023 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.61 No.3

The surge in the oil prices, increasing global population, climate change, and waste management problems are the major issues which have led to the development of biofuels from lignocellulosic wastes. Cellulosic or second generation (2G) bioethanol is produced from lignocellulosic biomass via pretreatment, hydrolysis, and fermentation. Pretreatment of lignocellulose is of considerable interest due to its influence on the technical, economic and environmental sustainability of cellulosic ethanol production. In this study, furniture waste sawdust was subjected to alkaline peroxide (H₂O₂) for the production of reducing sugars. Sawdust was pretreated at different concentrations from 1-3% H₂O₂ (v/v) loadings at a pH of 11.5 for a residence time of 15-240 min at 50, 75 and 90 ℃. Optimum pretreatment conditions, such as time of reaction, operating temperature, and concentration of H₂O₂, were varied and evaluated on the basis of the amount of total reducing sugars produced. It was found that the changes in the amount of lignin directly affected the yield of reducing sugars. A maximum of 50% reduction in the lignin composition was obtained, which yielded a maximum of 75.3% total reducing sugars yield and 3.76 g/L of glucose. At optimum pretreatment conditions of 2% H₂O₂ loading at 75 ℃ for 150 min, 3.46 g/L glucose concentration with a 69.26% total reducing sugars yield was obtained after 48 hr. of the hydrolysis process. Pretreatment resulted in lowering of crystallinity and distortion of the sawdust after the pretreatment, which was further confirmed by XRD and SEM results.

COLOR IMAGE PROCESSING ON A DIGITAL IMAGE

Satish Kumar Singh 한국디지털융합학회 2021 IJICTDC Vol.6 No.2

Color image processing is very useful because color is a strong marker that also simplifies the recognition and extraction of objects from a scene and humans can distinguish thousands of shades of color and intensity compared to just around two dozen shades of grey. The processing of color images can be classified into two main areas as full-color and pseudo-colour. The images are first acquired with a full color sensor such as color TV camera, color scanner and second category has the question of assigning a color to a different monochrome intensity and intensity ranges. The aim of a color model is to promote color specification in a widely agreed way to some standard. A color model is a coordinate system specification and a subspace within that system where a single point represents each color. Some particular conversions can convert different color models to one another. This paper specify overview of different types of images, color models, their conversion and applications.

hetADEEPS: ADEEPS for Heterogeneous Wireless Sensor Networks

Satish Chand,Samayveer Singh,Bijendra Kumar 보안공학연구지원센터 2013 International Journal of Future Generation Communi Vol.6 No.5

Moving load response on the stresses produced in an irregular microstretch substrate

Tanupreet Kaur,Satish Kumar Sharma,Abhishek Kumar Singh,Mriganka Shekhar Chaki 국제구조공학회 2016 Structural Engineering and Mechanics, An Int'l Jou Vol.60 No.2

The present article is aimed at an investigation of stresses produced in a microstretch elastic halfspace due to a moving load. The expressions of normal stress, shear stress and tangential couple stress produced in this case have been obtained in closed form. To find the displacement fields the perturbation method is applied. Significant effect of moving load on variation of stresses developed at different depths below the surface due to the depth of substrate and frictional coefficient of the rough surface of the medium has been observed. The effects of different shapes of irregularity and depth of irregularity on normal, shear and tangential couple stresses have been discussed. Some particular cases have also been deduced from the present investigation. Finally, the analytical developments have been illustrated numerically for aluminiumepoxy- like material substrate under the action of moving load.

Facile Synthesis of Bio-Composite Films Obtained from Sugarcane Bagasse and Cardboard Waste

Sanjeev Makin,Satish Kumar Singh,Sweety Verma,Himanshu Gupta,Avneesh Kumar Gehlaut,Suantak Kamsonlian,Surya Narain Lal,Ankur Gaur 한국화학공학회 2023 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.61 No.4

In this study, we focus on the recycling of cardboard waste and sugarcane bagasse (SCB) for the preparation of carboxymethyl cellulose (CMC) and its conversion into a biodegradable film. Sodium alginate (SA) was added to form a biodegradable composite film. SA was used to increase film permeability. Glycerol, which is a plasticizer, was used to increase the tensile strength (TS) and film expansion. To characterize the CMC, X-ray diffraction, Fourier transform infrared spectroscopy, and scanning electron microscopy were used. The addition of olive oil to the CMC-SA matrix highlighted its antimicrobial property against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). A slight decrease in tensile strength was observed with the addition of olive oil (OO), which improved the functional properties of the control films as well as lowered moisture content and water solubility. But considering all other factors, the composite films obtained from sugarcane bagasse and cardboard waste incorporated with olive oil are suitable for applications in the field of food packaging.

A novel approach for amine derivatization of MoS₂ nanosheets and their application toward label-free immunosensor

Kukkar, Manil,Tuteja, Satish K.,Kumar, Parveen,Kim, Ki-Hyun,Bhadwal, Akhshay Singh,Deep, Akash Elsevier 2018 Analytical Biochemistry Vol.555 No.-

<P><B>Abstract</B></P> <P>The application of molybdenum disulfide (MoS<SUB>2</SUB>) nanosheets has assumed great significance in the design of next-generation biosensors. The immobilization of biomolecules on MoS<SUB>2</SUB> nanosheets has generally been achieved via hydrophobic interactions or through other complicated surface modifications. In this work, we report a novel strategy for electrochemically assisted amine derivatization of MoS<SUB>2</SUB> nanosheets. This newly proposed approach helped to immobilize the MoS<SUB>2</SUB> nanosheets with antibodies via facile EDC/NHS {N-(3-dimethylaminopropyl)-N-(ethylcarbodiimide/N-hydroxysuccinimide)} cross-linking chemistry. The MoS<SUB>2</SUB> nanosheets were first exfoliated and then electrochemically modified with 2-aminobenzylamine. Through a subsequent bioconjugation of the above amine-derivatized MoS<SUB>2</SUB> nanosheets with anti-prostate-specific antigen (PSA) antibodies, an immunosensing device was realized for the detection of the ‘prostate specific antigen’. The application of the proposed immunosensor was characterized with a low detection limit (10<SUP>−3</SUP> ng/mL) over a very wide quantitation range (10<SUP>−3</SUP> to 200 ng/mL).</P> <P><B>Highlights</B></P> <P> <UL> <LI> The bioconjugation of MoS<SUB>2</SUB> nanosheets has been processed with a novel strategy. </LI> <LI> MoS<SUB>2</SUB> nanosheets were exfoliated and electrochemically modified with 2-aminobenzylamine. </LI> <LI> MoS<SUB>2</SUB> nanosheets have been interfaced with antibodies via facile EDC/NHS cross-linking chemistry. </LI> <LI> MoS<SUB>2</SUB> nanosheets immunosensor for PSA has offered a low detection limit and a very wide range of analysis. </LI> </UL> </P>

A comprehensive review on nano-molybdenum disulfide/DNA interfaces as emerging biosensing platforms

Kukkar, Manil,Mohanta, Girish C.,Tuteja, Satish K.,Kumar, Parveen,Bhadwal, Akhshay Singh,Samaddar, Pallabi,Kim, Ki-Hyun,Deep, Akash Elsevier 2018 Biosensors & Bioelectronics Vol.107 No.-

<P><B>Abstract</B></P> <P>The development of nucleic acid-based portable platforms for the real-time analysis of diseases has attracted considerable scientific and commercial interest. Recently, 2D layered molybdenum sulfide (2D MoS<SUB>2</SUB> from here on) nanosheets have shown great potential for the development of next-generation platforms for efficient signal transduction. Through combination with DNA as a biorecognition medium, MoS<SUB>2</SUB> nanostructures have opened new opportunities to design and construct highly sensitive, specific, and commercially viable sensing devices. The use of specific short ssDNA sequences like aptamers has been proven to bind well with the unique transduction properties of 2D MoS<SUB>2</SUB> nanosheets to realize aptasensing devices. Such sensors can be operated on the principles of fluorescence, electro-cheumuluminescence, and electrochemistry with many advantageous features (e.g., robust biointerfacing through various conjugation chemistries, facile sensor assembly, high stability with regard to temperature/pH, and high affinity to target). This review encompasses the state of the art information on various design tactics and working principles of MoS<SUB>2</SUB>/DNA sensor technology which is emerging as one of the most sought-after and valuable fields with the advent of nucleic acid inspired devices. To help achieve a new milestone in biosensing applications, great potential of this emerging technique is described further with regard to sensitivity, specificity, operational convenience, and versatility.</P> <P><B>Highlights</B></P> <P> <UL> <LI> In this review, an outlook of 2-D nanomaterial inspired biosensors is described. </LI> <LI> This article focused on MoS<SUB>2</SUB>-DNA/aptamer biosensors and their practical applications. </LI> <LI> 2-D MoS<SUB>2</SUB>-DNA interfaces are recognized as the next-generation biosensors. </LI> </UL> </P>

Impact of iron deficiency anemia on chronic periodontitis and superoxide dismutase activity: a cross-sectional study

Chakraborty, Souvik,Tewari, Shikha,Sharma, Rajinder Kumar,Narula, Satish Chander,Ghalaut, Pratap Singh,Ghalaut, Veena Korean Academy of Periodontology 2014 Journal of Periodontal & Implant Science Vol.44 No.2

Purpose: Both chronic periodontitis (CP) and iron deficiency anemia (IDA) induce oxidative stress in the body and cause an imbalance between reactive oxygen species and antioxidants, such as superoxide dismutase (SOD). This study explored the SOD enzyme activity of saliva and serum in CP patients with and without IDA and analyzed the impact of IDA on CP. Methods: A total of 82 patients were divided into four groups: control group (CG, 22), periodontally healthy IDA patients (IDA-PH, 20), CP patients (CP, 20), and IDA patients with CP (IDA-CP, 20). After clinical measurements and samplings, serum and salivary SOD levels were determined using an SOD assay kit. Results: IDA-CP patients exhibited a higher gingival index, bleeding on probing, probing pocket depth, and percentage (%) of sites with a clinical attachment loss (CAL) of ${\geq}6mm$ (P<0.008) than CP patients. The mean salivary and serum SOD levels were significantly lower in the IDA-PH, CP, and IDA-CP patients than in the CG group (P<0.008). A significant positive correlation between salivary and serum SOD activity was observed in IDA (P<0.05). Furthermore, serum and salivary SOD levels were significantly and negatively correlated with all periodontal parameters including the percentage of sites with CAL of 4-5 and ${\geq}6mm$ (P<0.05) except the significant correlation between salivary SOD activity and mean CAL and the percentage of sites with CAL of 4-5 mm (P>0.05) in these patients. Conclusions: Within the limits of this study, it may be suggested that IDA patients with chronic periodontitis have more periodontal breakdowns than patients with chronic periodontitis. Serum and salivary SOD activity levels were lower in the IDA-PH, CP and IDA-CP groups than in the CG. Iron deficiency anemia influenced the serum SOD activity but did not seem to affect the salivary SOD activity in these patients.

Impact of iron deficiency anemia on chronic periodontitis and superoxide dismutase activity: a cross-sectional study

Souvik Chakraborty,Shikha Tewari,Rajinder Kumar Sharma,Satish Chander Narula,Pratap Singh Ghalaut,Veena Ghalaut 대한치주과학회 2014 Journal of Periodontal & Implant Science Vol.44 No.2

Purpose: Both chronic periodontitis (CP) and iron deficiency anemia (IDA) induce oxidativestress in the body and cause an imbalance between reactive oxygen species and antioxidants,such as superoxide dismutase (SOD). This study explored the SOD enzyme activity ofsaliva and serum in CP patients with and without IDA and analyzed the impact of IDA on CP. Methods: A total of 82 patients were divided into four groups: control group (CG, 22), periodontallyhealthy IDA patients (IDA-PH, 20), CP patients (CP, 20), and IDA patients with CP(IDA-CP, 20). After clinical measurements and samplings, serum and salivary SOD levels weredetermined using an SOD assay kit. Results: IDA-CP patients exhibited a higher gingival index, bleeding on probing, probingpocket depth, and percentage (%) of sites with a clinical attachment loss (CAL) of ≥6 mm(P<0.008) than CP patients. The mean salivary and serum SOD levels were significantly lowerin the IDA-PH, CP, and IDA-CP patients than in the CG group (P<0.008). A significantpositive correlation between salivary and serum SOD activity was observed in IDA (P<0.05). Furthermore, serum and salivary SOD levels were significantly and negatively correlated withall periodontal parameters including the percentage of sites with CAL of 4–5 and ≥6 mm(P<0.05) except the significant correlation between salivary SOD activity and mean CAL andthe percentage of sites with CAL of 4–5 mm (P>0.05) in these patients. Conclusions: Within the limits of this study, it may be suggested that IDA patients withchronic periodontitis have more periodontal breakdowns than patients with chronic periodontitis. Serum and salivary SOD activity levels were lower in the IDA-PH, CP and IDA-CPgroups than in the CG. Iron deficiency anemia influenced the serum SOD activity but did notseem to affect the salivary SOD activity in these patients.