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Sequence Analysis of the Nuclear Matrix - Associated DNA Segments
Lee, Sang Eun,Park, Sang Dai,Lee, Chee Gun,Kim, Chan Kil 한국유전학회 1988 Genes & Genomics Vol.10 No.4
Nuclear matrix isolated from synchronized LP1-1 cells at G1/S boundary digested with DNase 1 to separate a nuclear-matrix(N.M)-associated DNA segments from the rest Through the cloning of the N.M-associated DNA segments (300-500 base pair in size) into a pUC18 vector, 800 clones (named as pMRs) were selected with X-Gal/IPTG and amphiciline. Putative clones harboring replication origin sequence were sequenced and characterized. We found that pMR15 and 16 act as a good template for origins of DNA replication in in vitro replication assay. Furthermore, pMR15 shows A/T richness and has a palindromic sequence. However, pMR62 shows G/C richness (65.7%) and has a homologous sequence to that of Adenovirus replication origin i.e. TGAPyGCC AGG.
( Kil Hyo Park ),( Soon Ha Kwon ),( Yong Sub Lee ),( Soung Won Jeong ),( Jae Young Jang ),( Sae Hwan Lee ),( Sang Gyune Kim ),( Sang Woo Cha ),( Young Seok Kim ),( Young Deok Cho ),( Hong Soo Kim ),( 대한간학회 2015 Clinical and Molecular Hepatology(대한간학회지) Vol.21 No.2
Background/Aims: The predictive role of contrast-enhanced ultrasonography (CEUS) before performing transarterial chemoembolization (TACE) has not been determined. We assessed the possible predictive factors of CEUS for the response to TACE. Methods: Seventeen patients with 18 hepatocellular carcinoma (HCC) underwent TACE. All of the tumors were studied with CEUS before TACE using a second-generation ultrasound contrast agent (SonoVue®, Bracco, Milan, Italy). The tumor response to TACE was classified with a score between 1 and 4 according to the remaining enhancing-tumor percentage based on modified response evaluation criteria in solid tumors (mRECIST): 1, enhancing tumor <25%; 2, 25%≤enhancing tumor<50%; 3, 50%≤enhancing tumor<75%; and 4, enhancing tumor≥75%). A score of 1 was defined as a “good response” to TACE. The predictive factors for the response to TACE were evaluated during CEUS based on the maximum tumor diameter, initial arterial enhancing time, arterial enhancing duration, intensity of arterial enhancement, presence of a hypoenhanced pattern, and the feeding artery to the tumor. Results: The median tumor size was 3.1 cm. The distribution of tumor response scores after TACE in all tumors was as follows: 1, n=11; 2, n=4; 3, n=2; and 4, n=1. Fifteen tumors showed feeding arteries. The presence of a feeding artery and the tumor size (≤5 cm) were the predictive factors for a good response ( P=0.043 and P=0.047, respectively).Conclusions: The presence of a feeding artery and a tumor size of less than 5 cm were the predictive factors for a good response of HCC to TACE on CEUS. (Clin Mol Hepatol 2015;21:158-164)
Topical formulation of retinyl retinoate employing nanostructured lipid carriers
Sang Gon Lee,Jae Han Jeong,Sung Rae Kim,Kyung Min Lee,Byeong Kil Ahn,Mean Hyung Kang,최영욱 한국약제학회 2012 Journal of Pharmaceutical Investigation Vol.42 No.5
Topical formulation of retinyl retinoate (RR)was developed with nanostructured lipid carriers (NLCs),composed of Compritol or Precirol as a solid lipid, canola oil as an oil, and Tween 80 as a surfactant. Hot high pressure homogenization method was efficiently employed to yield a homogenous nanodispersion in the size range of 230–300 nm with PDI values of\0.2, regardless of lipid selection. Precirol-based NLC (P-NLC) showed higher drug entrapment than that of Compritol-based NLC (C-NLC): RR encapsulation efficiency (%) of P- and C-NLC was 97.8 and 93.8 in average, respectively; drug loading (mg RR/g lipid)was 89.6 and 83.3 in average, respectively. Processing condition at relatively low temperature of 60 C was a key factor for maintaining RR stability. Drug release of P-NLC was greater than that of C-NLC, even though both NLCs revealed controlled release pattern. Therefore, P-NLC system was considered as a suitable vehicle for topical drug delivery, especially for heat-labile ingredient like RR.
Sang-Guen Jung(정상근),Rheo B. Lamorena, Woo Jin Lee(이우진),Gwi-Nam Bae(배귀남),Kil-Choo Moon(문길주),Shin-Do Kim(김신도) 한국실내환경학회 2004 한국실내환경학회지 Vol.1 No.1
입자, 알데히드류, 케톤 같은 유해한 휘발성 유기화합물들이 생성된다는 보고가 있었다. 이번 연구에서는 천연 페인트에서 방출되는 털핀과 오존의 반응에 의한 미세 입자와 이차 유기화합물 생성에 대해서 조사하였다. 시편에 페인트를 칠하여 실내에서 건조시킨 후 테플론 챔버 내에서 오존과 반응시켰다. α, β-파이닌은 GC-MS와 FTIR을 사용하여 정성하였다. 입자생성에 대한 오존의 영향을 조사하기 위해 여러가지 실험이 수행되었다. 오존 농도가 100ppb에서 1000ppb로 증가할 때 입자 수농도는 8,000에서 70,000 particles/㎤까지 증가하였다. 포름알데하이드, 아세트알데하이드, 아세톤+아크로레인, 프로피온알데하이드 등의 반응 생성물은 HPLC로 분석하였다. 이런 화합물들은 잠재적으로 유해한 화합물이고, 인체에 해로운 영향을 끼친다. 이번 연구결과는 친환경제품의 실내공기질에 대한 해로운 영향에 대한 보기를 보여주었다. The use of natural paint for the application to walls and furnishings is now increasing to improve indoor air quality, thereby the natural paint could be a significant source of biogenic volatile organic compounds (BVOCs) in indoor environments. Recent studies have shown that gas-phase reactions between terpenes and ozone can generate sub-micron size particles and toxic volatile organic compounds such as aldehydes and ketones. In this research, we have studied the formation of particles and secondary organic compounds during the reaction of ozone with terpenes emitted from commercial natural paint. The paint applied onto stainless steel was dried and oxidized in a teflon chamber. Two monoterpenes (α- and β-pinenes) were identified by FTIR and GC/MS. Several tests were performed to evaluate the effects of ozone concentration on particle formation. Increased ozone levels significantly affect the increase of particle number concentration (monitored with SMPS), which results in the increase of particle counts ranging from 8,000 to 70,000 particles/㎤. Gas-phase products such as formaldehyde, acetaldehyde, acetone + acrolein, and propionaldehyde were identified during the terpene/ozone reactions by HPLC. These compounds are potential hazardous chemical compounds having harmful health effects to animals and plants. The results obtained from this study provide an insight on the adverse effect of eco-friendly natural product on indoor air quality (IAQ).
PCR-Based Detection of Mycoplasma Species
Sung Hyeran,Kang Seung Hye,Bae Yoon Jin,Hong Jin Tae,Chung Youn Bok,Lee Chong-Kil,Song Sukgil The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.1
In this study, we describe our newly-developed sensitive two-stage PCR procedure for the detection of 13 common mycoplasmal contaminants (M. arthritidis, M. bovis, M. fermentans, M. genitalium, M. hominis, M. hyorhinis, M. neurolyticum, M. orale, M. pirum, M. pneumoniae, M. pulmonis, M. salivarium, U. urealyticum). For primary amplification, the DNA regions encompassing the 16S and 23S rRNA genes of 13 species were targeted using general mycoplasma primers. The primary PCR products were then subjected to secondary nested PCR, using two different primer pair sets, designed via the multiple alignment of nucleotide sequences obtained from the 13 mycoplasmal species. The nested PCR, which generated DNA fragments of 165-353 bp, was found to be able to detect 1-2 copies of the target DNA, and evidenced no cross-reactivity with the generated DNA of related microorganisms or of human cell lines, thereby confirming the sensitivity and specificity of the primers used. The identification of contaminated species was' achieved via the performance of restriction fragment length polymorphism (RFLP) coupled with Sau3AI digestion. The results obtained in this study furnish evidence suggesting that the employed assay system constitutes an effective tool for the disagnosis of mycoplasmal contamination in cell culture systems.
cDNA Sequences for Asialoglycoprotein Receptor from Human Fetal Liver
Lee, Dong-Gun,Lee, Sung-Gu,Kim, Kil-Lyong,Hahm, Kyung-Soo Korean Society for Biochemistry and Molecular Biol 1997 Journal of biochemistry and molecular biology Vol.30 No.4
The asialoglycoprotein receptor (ASGPR) was the first described mammalian lectin that mediates the specific binding and internalization of galactose/N-acetylgalactosamine-terminating glycoproteins by hepatic parenchymal cells. H1 and H2 are known as essential subunits of the functional ASGPR. There were close similarities in ASGPR H2 subunits between cultured cell line HepG2 and normal human liver cells including identical sequences at both termini. It was therefore expected that there may be some similarities between the subunits from normal liver cells and fetal liver cells. The two subunits of human fetal liver ASGPR. designated FL-H1 and FL-H2. were cloned from cDNA library by peR and the sequences were compared with the known HI and H2 sequences of HepG2, and the H1 sequence of nornal human liver cells. The results showed that FL-H1 was identical to H1 of HepG2. Whereas FL-H2 contains a 15-bp miniexon, but missing 57-bp at the near upstream from the membrane-spanning domain compared to H2 of HepG2 and normal human liver cells indicating that FL-H2 resulted from a differential splicing compared to HepG2 and normal liver cells.
( Sang Hyun Yoon ),( Da Hyun Jung ),( Jun Chul Park ),( Sung Kwan Shin ),( Sang Kil Lee ),( Yong Chan Lee ),( Hyun Soo Chung ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Background: Rate of cancer-positive lateral margin (LM) after endoscopic submucosal dissection (ESD) for early gastric cancer (EGC) have been reported 2 to 12%. Sometimes cancer cells abut to multiple lateral margins. The aim of this study was to compare clinicopathologic characteristics and treatment outcomes between single and multiple cancer-positive LM group. Methods: From September 2010 to April 2014, 1346 patients underwent ESD for EGC. Among them, 33 patients (2.5%) had cancer-positive LM. Clinicopathological data of these patients were investigated. Results: There were 25 patients with single positive LM (group LM1+) and 8 with multiple positive LM (group LM2+). No patients met the absolute indication of ESD, and all patients met the expanded indication (n=17) or beyond indication (n=16). LM2+ group tended to have more undifferentiated carcinoma (62.5% vs. 36.0%) and larger size of the lesion (>30mm) (62.5% vs. 28%), but there were no statistical signifi cance. Sixteen patients (64%) and 1 patient (12.5%) met expanded indication of ESD in LM1+ and LM2+ group, respectively. After ESD, 5 patients (20.0%) and 1 patient (12.5%) underwent regular endoscopic follow-up, and 11 patients (36.4%) and 1 patient (12.5%) underwent local control with redo-ESD or argon plasma coagulation in LM1+ and LM2+ group, respectively. There were no locoregional or distant metastasis during the follow-up periods (median 25.1 months). Nine (36.0%) in LM1+ group and 6 (75.0%) in LM2+ group underwent surgery after ESD. Residual cancer was detected in 69.2% (9/13) and 66.7% (4/6) after surgery or redo-ESD in LM1+ and LM2+ group, respectively. Conclusions: Even though there was no statistical signifi cance, proportions of large lesion size (>3cm) and undifferentiated carcinoma were higher in LM2+ group than LM1+ group. Careful investigation of margin is necessary for these patients.