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Sampath Natarajan,강린우,김진광,Tae-Kyun Jung,Thanh Thi Ngoc Doan,Ho-Phuong-Thuy Ngo,홍명기,Seunghwan Kim,Viet Pham Tan,Seok Joon Ahn,이상희,한예선,안예진 한국분자세포생물학회 2012 Molecules and cells Vol.33 No.1
Xanthomonas oryzae pv. oryzae (Xoo) is a plant bacterial pathogen that causes bacterial blight (BB) disease, resulting in serious production losses of rice. The crystal structure of malonyl CoA-acyl carrier protein transacylase (XoMCAT), encoded by the gene fabD (Xoo0880) from Xoo, was determined at 2.3 Å resolution in complex with N-cyclohexyl-2-aminoethansulfonic acid. Malonyl CoA-acyl carrier protein transacylase transfers malonyl group from malonyl CoA to acyl carrier protein (ACP). The transacylation step is essential in fatty acid synthesis. Based on the rationale, XoMCAT has been considered as a target for antibacterial agents against BB. Protein-protein interaction between XoMCAT and ACP was also extensively investigated using computational docking, and the proposed model revealed that ACP bound to the cleft between two XoMCAT subdomains.
Sampath M B,Prakash C,Senthil Kumar M 한국섬유공학회 2019 Fibers and polymers Vol.20 No.3
In this study an attempt has been made to study the influence of laundry performance on the various comfortcharacteristics of Moisture Management Finished (MMF) knitted fabrics. The influence of laundering on comfortcharacteristics of MMF microdenier polyester knitted fabrics was studied to find out the durability of the MMF and itscapability to withstand at least 20 home laundry cycles. In order to study this effect, the MMF knitted fabrics were subjected to different laundering cycles. It was observed that 10 wash and 20 wash fabrics exhibited a superior performance thereby fulfilling an important parameter of permanent wash proofness.
Novel Antioxidants and Atherosclerosis
Sampath Parthasarathy,Nalini Santanam 한국식품영양과학회 2004 한국식품영양과학회 학술대회발표집 Vol.55 No.-
Coronary heart disease (CHD) has been the number one killer in western society for a long time, and CHD in most instances is due to atherosclerosis. One of the earliest events in atherogenesis is the intracellular accumulation of lipids, particularly cholesterol esters, in the aortic intima. The lipids presumably came from the uptake of plasma lipoproteins, particularly from LDL. These foam cells were identified as being predominantly as macrophages. Currently, it is believed that oxidation of low density lipoprotein (LDL) might contribute to the generation of foam cells. An outcome of the oxidation hypothesis is that the consumption of antioxidants would be beneficial. In this study, Boldine, an alkaloid of Peumus boldus was tested for their antioxidant potency both in, in vitro oxidation system and in mouse models. Boldine decreased the ex-vivo oxidation of Low-density lipoprotein (LDL). In vivo studies were performed to study the effect of these compounds on the atherosclerotic lesion formation in LDL r-/- mice. Three groups of LDL r-/- mice (N=12 each) were fed an atherogenic diet. Group 1 was given vehicle and group 2 and 3 were given 1 and 5 mg of Boldine/day in addition to the atherogenic diet. The results indicated that there was a decrease in lesion formation reaching a 40% reduction due to Boldine compared to controls. The in vivo tolerance of Boldine in humans (has been used as an herbal medicine in other diseases) should make it an attractive alternative to vitamin E.
Sampath Suranjan Salins,Shiva Kumar,S. V. Kota Reddy 대한설비공학회 2020 International Journal Of Air-Conditioning and Refr Vol.28 No.1
Desiccant cooling mechanism is one of the alternate methods to control the humidity of air and temperature, compared to the conventional vapor compression method of air conditioning. Desiccant cooling doesn’t use harmful chemicals which effect the ozone layer and it saves lots of energy. Summer air condition system can use this technology since it removes the latent heat load from the room effectively and this process is economical. Selection of the appropriate liquid desiccant and packing material is very vital to obtain maximum dehumidification. This paper focuses on the different desiccants and packings used by different researchers to enhance the dehumidification. Simple and hybrid systems are also reviewed, and their comparison are presented based on the construction and dehumidification performance.
Cluster-based Routing Method for Wireless Sensor Networks with Heterogeneous Node Types
Sampath Priyankara,Kazuhiko Kinoshita,Hideki Tode,Koso Murakami 한국멀티미디어학회 2009 한국멀티미디어학회 국제학술대회 Vol.2009 No.-
With advances of low-cost hardware such as CMOS cameras and microphones, multimedia sensor networking technologies have developed very rapidly in recent years. Maintaining higher alive node ratio and wider coverage area is indispensable for ubiquitous retrieval of multimedia contents such as video and audio streams, still images and scalar sensor data from the environment. To achieve those requirements, sensor network systems are gradually moving into heterogeneous designs, incorporating a variety of sensor nodes. In this paper, we propose a new cluster-based routing method for wireless sensor networks with heterogeneous node types. It selects cluster heads considering not only transmission power and residual energy of each node but also those of its adjacent nodes. Simulation results show that the proposed method significantly increases network lifetime and also maintains high coverage rate in comparison with existing methods.
Comparative analysis of <i>Cassandra</i> TRIMs in three Brassicaceae genomes
Sampath, Perumal,Yang, Tae-Jin Cambridge University Press 2014 Plant genetic resources Vol.12 No.1
<P>Terminal-repeat retrotransposon in miniature (TRIM) elements are a miniature form of retrotransposons and play an important role in genome organization. The <I>Cassandra</I> TRIM family has been identified in over 50 plant species, including both monocots and dicots. <I>Cassandra</I> elements carry an independently transcribed 5S RNA sequence in their terminal repeat regions, which is unique compared with other TRIM families. Although the existence of <I>Cassandra</I> elements has been documented in many plants, much work remains to characterize <I>Cassandra</I> family members and elucidate their distribution. In this study, we comparatively analysed the <I>Cassandra</I> family members in the <I>Brassica oleracea</I>, <I>B. rapa</I> and <I>Arabidopsis thaliana</I> genomes. A total of 602, 451 and 173 members, of which 130, 60 and 9 were relatively intact, were identified from the <I>B. oleracea</I>, <I>B. rapa</I> and <I>A. thaliana</I> genomes, respectively. Most of the <I>Cassandra</I> elements (1120/1226) were found in intergenic spaces, but 106 elements were inserted in genic regions such as introns, exons and untranslated regions. Our comparative analysis of the <I>Cassandra</I> family members in <I>A. thaliana</I>, <I>B. rapa</I> and <I>B. oleracea</I> reveals that some <I>Cassandra</I> elements have been commonly retained during the last 20 million years in three species and some elements have been uniquely evolved in <I>Brassica</I> species. This study promotes our understanding of the role and utility of <I>Cassandra</I> elements in the evolution of the Brassicaceae family.</P>
Sampath Perumal,Jee Young Park,Shailendra Karki,Hong-Il Choi,Jonghoon Lee,Nur Kholilatul Izzah,Yun-Sun Lee,Nam Hoon Kim,Ju Yoen Jung,Jun Ki Lee,Hyun-Seung Park,Hye Mi Park,Beom-Seok Park,Tae-Jin Yang 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04
family in the Brassica genome sequences by computational approach. The MITE family showed a total of 264bp length including 36bp terminal inverted repeats and remained 2bp (TA) targets it eduplication by its insertion. By searching the genome database of Brassica species, 516, 227, and 15 members were identified from 470Mbp of Brassica oleraceae, 154Mbp of B.rapa and 15Mbp of B.napus, respectively, indicating that there are approximately 692, 760, 1235 copies in B.oleracea, B.rapa and B.napus genomes,respectively. A total of 225 relatively intact MITE members, 146,68, and 11 members, which showed >80% sequence similarity and sequence coverage were identified and retrieved for MITE analysis from B.oleracea, B.rapa and B.napus genomes, respectively. Out of 225 MITE family members 159 having full structure of MITE and 66 having the truncated end either in right TIR or left TIR. Insertion polymorphism due to insertion or non-insertion of MITEs showed high level of polymorphism among accessions intra and inter species of Brassica. The new MITE would provide abetter tool for study molecular breeding in Brassica species and also helpful to understand their contribution in evolution and diversification of the highly duplicated Brassica genome.
Inhibition of Atherosclerosis in Low-Density Lipoprotein Receptor-Negative Mice by Sesame Oil
Sampath Parthasarathy,Shylesh Bhaskaran,Nalini Santanam,Meera Penumetcha 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.4
Diet has profound effects on the development of atherosclerosis. Fatty acid composition, antioxidants, andother components such as lignans have major effects on the atherosclerotic process. Sesame oil has both mono- and polyun-saturated fatty acid constituents in equal proportions. In addition, it also has high levels of numerous antioxidants and induc-ers of peroxisome proliferator-activated receptor. The objective of this study was to determine the anti-atherosclerotic effectsof sesame oil. In this study, male low-density lipoprotein (LDL) receptor (LDLR) . /. mice were fed atherogenic diet oratherogenic diet reformulated with the same level of sesame oil (sesame oil diet). Plasma lipids and atherosclerotic lesionswere quantified after 3 months of feeding. Sesame oil-containing diet significantly reduced the atherosclerotic lesion forma-tion and plasma cholesterol, triglyceride, and LDL cholesterol levels in LDLR . /. mice. These findings suggest that sesameoil could inhibit atherosclerosis lesion formation effectively, perhaps because of the synergistic actions of fatty acid and non-saponifiable components.