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( Sabina Shrestha ),( Ji Hae Park ),( Dae Young Lee ),( Jin Gyeong Cho ),( Jong Su Yoo ),( Seung Woo Yeon ),( Hee Cheol Kang ),( Nam In Baek ) 한국응용생명화학회(구 한국농화학회) 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.6
Stigmast-5-en-3β,7β-diol (1), stigmast-5-en-3β,7α-diol (2), (+)-isolariciresinol-9-O-β-D-xylopyranoside (3), procyanidin B2 (4), and epicatechin-(4β→8)-epicatechin-(4β→6)-epicatechin (5) were isolated for the first time from the bark of Machilus thunbergii. The chemical structures of the compounds were determined based on nuclear magnetic resonance, electronic ionization mass spectrometry, fast atom bombardment mass spectrometry, and IR. Compound 4 demonstrated inhibitory activity against cyclin-dependent kinases (CDK2, CDK4, and CDK5) in vitro with IC50 values 17.22, 1.08, and 0.26 μM, respectively.
Shrestha, Sabina,Park, Ji-Hae,Lee, Dae-Young,Cho, Jin-Gyeong,Cui, En-Ji,Chung, In-Sik,Kwon, Byoung-Mog,Cho, Moon-Hee,Jeong, Tae-Sook,Baek, Nam-In The Korean Society for Applied Biological Chemistr 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
A new xanthene was isolated from the methanolic extract of Desmostachya bipinnnata through repeated silica gel and octadecyl silica gel column chromatographies. Based on spectroscopic data including nuclear magnetic resonance, fast atom bombardment mass spectrometry, and infrared spectroscopy, chemical structure of the compound was determined to be 2,6-dihydroxy-7-methoxy-3H-xanthen-3-one. The compound exhibited inhibitions of signal transducer and activator of transcription 3-dependent luciferase activity in HCT-116 colon cancer cell line with $IC_{50}$ value of $5{\mu}M$ and low-density lipoprotein-oxidation with $IC_{50}$ value of $27.2{\mu}M$.
( Sabina Shrestha ),( Ji Hae Park ),( Dae Young Lee ),( Jin Gyeong Cho ),( Do Gyeong Lee ),( Moon Hee Cho ),( Tae Sook Jeong ),( Hee Cheol Kang ),( Nam In Baek ) 한국응용생명화학회(구 한국농화학회) 2011 Journal of Applied Biological Chemistry (J. Appl. Vol.54 No.1
The bark of Machilus thunbergii was extracted with 80% aqueous methanol (MeOH), and the concentrated extract was partitioned using ethyl acetate (EtOAc), butanol (n-BuOH), and H2O, successively. From the EtOAc fraction, five lignans were isolated through the repeated silica gel, octadecyl silica gel (ODS) and, Sephadex LH-20 column chromatography. Based on nuclear magnetic resonance (NMR), mass spectroscopy (MS), and infrared spectroscopy (IR) spectroscopic data, the chemical structures of the compounds were determined to be machilin A (1), machilin F (2), licarin A (3), nectandrin A (4), and nectandrin B, (5). This study presents comparative account of five lignans from M. thunbergii bark contributing inhibition of low density lipoprotein (LDL), ACAT-1, and ACAT-2. Compounds 2-5 showed varied degree of antioxidant activity on LDL with IC50 values of 2.1, 11.8, 15.3, and 4.1 μM. Compounds 1, 2, and 3 showed inhibition activity on ACAT-1 with values 63.4±6.9% (IC50=66.8 μM), 53.7±0.9% (IC50=109.2 μM), and 78.7±0.2% (IC50= 40.6 μM), respectively, at a concentration of 50 mg/mL, and on ACAT-2 with values 47.3±1.5% (IC50=149.7 μM), 39.2±0.2% (IC50=165.2 μM), and 52.1±1.0% (IC50=131.0 μM), respectively, at a concentration of 50 mg/mL.
Sabina Shrestha,이대영,박지해,조진경,이동성,Bin Li,김윤철,전용진,연승우,백남인 한국식품과학회 2013 Food Science and Biotechnology Vol.22 No.4
Nepalese sumac (Rhus parviflora) is a wild edible fruit used for the treatment of various ailments including neurological complications and stomach disorders in the traditional medicinal system of south Asia (Ayurveda). Four flavonoids were isolated from ethyl acetate (EtOAc)fraction of Nepalese sumac fruits and their chemical structures were determined on the basis of NMR, fast atom bombardment mass spectrometry (FAB/MS), and IR. The efficiency of isolated compounds in attenuating glutamateinduced cell death in an immortalized mouse hippocampal cell line (HT-22) and inhibition of cycline dependent kinase 5 (Cdk5) were investigated. Among the compounds,flavanols, fustin (1) and taxifolin (2), an aurone, aureusidin (3), and a biflavonoid, agathisflavone (4) were found to have protective effect against glutamate induced oxidative injury in HT22 cells. Aureusidin (3), a Cdk5/p25 inhibitor (IC50 3.5 μM), was the most potent neuroprotector with an EC50 value of 11.90 μM.
( Sabina Shrestha ),( Ji Hae Park ),( Jin Gyeong Cho ),( Dae Young Lee ),( Ji Hyun Kang ),( Hua Li ),( Tae Sook Jeong ),( Somi Kim Cho ),( Dong Sun Lee ),( Nam In Baek ) 한국응용생명화학회(구 한국농화학회) 2015 Journal of Applied Biological Chemistry (J. Appl. Vol.58 No.2
Fruits of Rhus parviflora were extracted with 80% aqueous methanol (MeOH), and the concentrated extract was partitioned using ethyl acetate (EtOAc), n-butanol (n-BuOH), and H2O, successively. Purification of EtOAc fraction led to isolation of fifteen polyphenols of which structures were identified by spectroscopic methods including 2D-NMR. Most compounds apart from compound 10 inhibited low density lipoproteinoxidation within IC50 value of 10 μM. Among compounds, taxifolin (2), quercetin 3-O-α-L-rhamnopyranoside (13), agathisflavone (5) sulfuretin (4), and aureusidin (3) showed IC50 values 0.9, 0.8, 5.8, 2.9, and 2.4 μM which were of highly significant in comparison positive control butylated hydroxytoluene with IC50 value of 2.1 μM. The results indicate fruits of R. parviflora as a source of antihypercholesterolemic compounds.
Shrestha, Sabina,Lee, Dae-Young,Park, Ji-Hae,Cho, Jin-Gyeong,Seo, Woo-Duck,Kang, Hee Cheol,Jeon, Yong-Jin,Yeon, Seung-Woo,Bang, Myun-Ho,Baek, Nam-In The Korean Society for Applied Biological Chemistr 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.5
Chrysoeriol-7-O-${\beta}$-D-glucopyranoside (1), luteolin-7-O-${\beta}$-D-glucopyranoside (2), quercetin-3-O-${\beta}$-D-glucopyranoside (3), quercetin-3-O-${\beta}$-D-galactopyranoside (4), and quercetin-3-O-${\alpha}$-L-rhamnopyranoside (5) were isolated for the first time from the fruits of Rhus parviflora. The chemical structures of the compounds were determined using nuclear magnetic resonance, fast atom bombardment mass spectrometry, and infrared spectroscopy. Compound 4 (hyperin) inhibited cyclin dependent kinases (CDK2 and CDK5) in vitro with $IC_{50}$ values of 21.02 and $10.28{\mu}M$, respectively.
Cytotoxic and Neuroprotective Biflavonoids from the Fruit of Rhus parviflora
Shrestha, Sabina,Park, Ji-Hae,Lee, Dae-Young,Cho, Jin-Gyeong,Seo, Woo-Duck,Kang, Hee Cheol,Yoo, Ki-Hyun,Chung, In-Sik,Jeon, Yong-Jin,Yeon, Seung-Woo,Baek, Nam-In The Korean Society for Applied Biological Chemistr 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.4
Six biflavonoids, succedaneaflavanone (1), mesuaferrone B (2), rhusflavanone (3), rhusflavone (4), agathisflavone (5), and cupressuflavone (6), were isolated from the fruits of Rhus parviflora. The chemical structures of the compounds were determined based on NMR, fast atom bombardment mass spectrometry, and IR. Biflavonoid compounds were evaluated for cytotoxicity against human cancer cell lines, including human colon carcinoma (HCT-116), human breast carcinoma (MCF-7), and human cervical carcinoma (HeLa). Biflavonoids 2, 3, and 5 showed significant cytotoxicity with $IC_{50}$ values of $17.25{\mu}M$ (mesuaferrone B against HCT-116), $17.50{\mu}M$ (rhusflavone against MCF-7), and $15.20{\mu}M$ (agathisflavone against HeLa). Compound 5 showed inhibition of ${\beta}$-secretase activity at a $10{\mu}M$ concentration. Compound 6 showed inhibition of cyclin-dependent kinases (CDK2 and CDK5) with $IC_{50}$ values of 18.58 and $9.29{\mu}M$, respectively.
Sabina Shrestha,박지해,이대영,Jin-Gyeong Cho,Jong-Su Yoo,Seung-Woo Yeon,Hee Cheol Kang,백남인 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.6
Stigmast-5-en-3β,7β-diol (1), stigmast-5-en-3β,7α-diol (2), (+)-isolariciresinol-9-O-β-D-xylopyranoside (3), procyanidin B2 (4), and epicatechin-(4β→8)-epicatechin-(4β→6)-epicatechin (5) were isolated for the first time from the bark of Machilus thunbergii. The chemical structures of the compounds were determined based on nuclear magnetic resonance, electronic ionization mass spectrometry, fast atom bombardment mass spectrometry, and IR. Compound 4 demonstrated inhibitory activity against cyclin-dependent kinases (CDK2, CDK4, and CDK5) in vitro with IC50 values 17.22, 1.08, and 0.26 μM, respectively.
Sabina Shrestha,Ji-Hae Park,이대영,Jin-Gyeong Cho,En-ji Cui,정인식,Byoung-Mog Kwon,Moon-Hee Cho,Tae-Sook Jeong,Nam-In Baek 한국응용생명화학회 2011 Applied Biological Chemistry (Appl Biol Chem) Vol.54 No.2
A new xanthene was isolated from the methanolic extract of Desmostachya bipinnnata through repeated silica gel and octadecyl silica gel column chromatographies. Based on spectroscopic data including nuclear magnetic resonance, fast atom bombardment mass spectrometry, and infrared spectroscopy, chemical structure of the compound was determined to be 2,6-dihydroxy-7-methoxy-3H-xanthen-3-one. The compound exhibited inhibitions of signal transducer and activator of transcription 3-dependent luciferase activity in HCT-116 colon cancer cell line with IC50 value of 5 μM and low-density lipoprotein-oxidation with IC50 value of 27.2 μM.
Sabina Shrestha,이대영,Ji-Hae Park,Jin-Gyeong Cho,Woo-Duck Seo,Hee Cheol Kang,Yong-Jin Jeon,Seung-Woo Yeon,Myun-Ho Bang,백남인 한국응용생명화학회 2012 Applied Biological Chemistry (Appl Biol Chem) Vol.55 No.5
Chrysoeriol-7-O-β-D-glucopyranoside (1), luteolin-7-O-β-D-glucopyranoside (2), quercetin-3-O-β-D-glucopyranoside (3), quercetin-3-O-β-D-galactopyranoside (4), and quercetin-3-O-α-L-rhamnopyranoside (5) were isolated for the first time from the fruits of Rhus parviflora. The chemical structures of the compounds were determined using nuclear magnetic resonance,fast atom bombardment mass spectrometry, and infrared spectroscopy. Compound 4 (hyperin) inhibited cyclin dependent kinases (CDK2 and CDK5) in vitro with IC50 values of 21.02 and 10.28 μM, respectively.