Detection of Genital HPV Infection Using Urine Samples: a Population Based Study in India

Sabeena, Sasidharanpillai,Bhat, Parvati,Kamath, Veena,Mathew, Mary,Aswathyraj, Sushama,Devadiga, Santhosha,Prabhu, Suresha,Hindol, Maity,Chameetachal, Akhil,Krishnan, Anjana,Arunkumar, Govindakarnavar Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.3

Background: Cervical cancer is the second commonest cancer among Indian women and its association with human papilloma virus (HPV) is well established. This preventable cancer accounts for the maximum number of cancer related deaths among rural Indian women. Unlike in developed countries there are no organized cervical cancer screening programmes in India due to lack of resources and manpower. Objective: To detect genital HPV infection using urine samples among asymptomatic rural women in the age group of 18-65 years. Materials and Methods: The study area chosen was Perdoor village in Udupi Taluk, Karnataka State and all the women in the age group of 18-65 years formed the study cohort. A cross sectional study was conducted by house visits and 1,305 women were enrolled in the study. After taking written informed consent a data sheet was filled and early stream random urine samples were collected, transported to a laboratory at 4OC and aliquoted. Samples were tested using nested HPV PCR with PGMY09/11 and GP5+/6+ primers. Positive cases were genotyped by sequence analysis. Results: Study participants included 1,134 sexually active and 171 unmarried women with a mean age at marriage of 22.1 (SD=3.9) years. Study area showed high female literacy rate of 86.6%. Five urine samples tested positive for HPV DNA (0.4%). Conclusions: We found very low genital HPV infection rate among women from monogamous community. This is the first major population based study carried out among asymptomatic rural women to detect genital HPV infectio from Karnataka using urine samples.

Knowledge, Attitude and Practice Concerning Human Papilloma Virus Infection and its Health Effects among Rural Women, Karnataka, South India

Sabeena, Sasidharanpillai,Bhat, Parvati V,Kamath, Veena,Aswathyraj, Sushama,Arunkumar, Govindakarnavar Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.12

Background: Cervical cancer is one of the commonest cancers among women all over the world. The association of cervical cancer with human papilloma virus (HPV) is well established. Knowledge about the causal relationship between HPV and cervical cancer is important to make appropriate, evidence-based health care choices. In this context we conducted a community based study among women about the knowledge, attitude and practice about HPV infections and their health effects. Materials and Methods: A cross sectional interview based house to house survey was conducted with a validated data collection tool covering sociodemographic factors, knowledge, attitude and practice about HPV and its health effects, among 1020 women from a rural village, Perdoor, in Udupi district, Karnataka, India in 2013-14. Results: The mean age of participants was 38.9 years (SD=12.6). Study participants showed a high literacy rate (85.7%). Only 2.4% of sexually exposed women had undergone Pap smear testing. Partners of 4.4%women had undergone circumcision and they belonged to the Muslim community. Male condom usage was reported by 26 women (2.6%). However, none of the participants had heard of HPV and its health effects. Conclusions: This community based study found complete ignorance about HPV among rural South Indian women in spite of a high literacy level.

A comprehensive review of phytochemistry, pharmacology and toxicology of the genus Aconitum L.

Sabeena Ali,Rekha Chouhan,Phalisteen Sultan,Qazi Parvaiz Hassan,Sumit G. Gandhi 경희대학교 융합한의과학연구소 2023 Oriental Pharmacy and Experimental Medicine Vol.23 No.2

The genus Aconitum (family—Ranunculaceae) accounts for approximately 400 species worldwide, of which some are considered medicinally very relevant. Many species have been reported to possess significant pharmacological properties and high therapeutic index for curing various diseases. Traditionally, Aconitum species have been used for the treatment of different human ailments, especially, neuronal disorders, pain and inflammation, kidney related disorders, rheumatism, diabetes, hysteria and cardiac depression. These medicinal properties have been attributed to various classes of secondary metabolites found in different Aconitum species. Among them, C19 and C20-diterpenoid alkaloids are the most dynamic group of compounds, comprising of more than 450 alkaloids in various species across the genus. However, further detailed phytochemical investigations of Aconitum species could provide new therapeutic natural products of Aconitum source that could be employed in health and medicine. The present study aims to frame a well structured portfolio to provide contemporary insights of the genus Aconitum. The retrospection of all the available information on Aconitum associated with its various attributes was carried out by searching different electronic databases viz. Elsevier, PubMed, Web of Science, Scifinder, ScienceDirect, ResearchGate, Google Scholar, Bio One, PLOS. The review summarizes the findings on traditional uses, phytochemistry, and pharmacology of various species of the genus. The toxicology and safety of Aconitum species for human consumption as herbal drugs has also been discussed.

Human papillomavirus (HPV) DNA detection in uterine cervix cancer after radiation indicating recurrence: a systematic review and meta-analysis

Sasidharanpillai Sabeena,Santhosh Kuriakose,Binesh Damodaran,Nagaraja Ravishankar,Govindakarnavar Arunkumar 대한부인종양학회 2020 Journal of Gynecologic Oncology Vol.31 No.2

Objective: The causal association of human papillomavirus (HPV) in uterine cervical cancer was well established and this oncogenic virus was reported to be a biomarker for overall recurrence and central pelvic recurrence. The objective of the present systematic review and meta-analysis was to assess the role of HPV DNA testing in early detection of recurrence among cervical cancer survivors after radiotherapy. Methods: We performed a systematic review and meta-analysis by means of searching electronic databases for published articles between January 1984 and June 2018, on the basis of standard systematic review guidelines prescribed by major agencies namely Cochrane Collaboration (https://www.cochrane.org) and Campbell Collaboration (https://www.campbellcollaboration.org). The meta-analysis component was further modified appropriately for the synthesis of sensitivity and specificity results. Results: A total of 1,055 cervical cancer cases who had received pelvic radiation with or without chemotherapy from ten cohort studies were evaluated. The overall pooled sensitivity and specificity of HPV DNA testing was 0.84 (95% confidence interval [CI]= 0.66–0.94) and 0.35 (95% CI=0.20–0.54) respectively. The positive likelihood ratio was 1.3 (95% CI=1.0–1.7) and the negative likelihood ratio was 0.45 (95% CI=0.18–1.10) with an estimated diagnostic odds ratio of 3 (95% CI=1–9). Conclusion: The screening for HPV DNA testing during follow-up facilitates early detection of recurrence after radiotherapy.

Cardioprotective Effect of Squalene on Lipid Profile in Isoprenaline-Induced Myocardial Infarction in Rats

R. Anandan,K.H. Sabeena Farvin,S. Hari Senthil Kumar,K.S. Shiny,Suseela Mathew,T.V. Sankar,P.G. Viswanathan Nair 한국식품영양과학회 2006 Journal of medicinal food Vol.9 No.4

We studied the cardioprotective effect of squalene on isoprenaline-induced myocardial infarction in male al-bino rats with respect to changes in the levels of lipid components in plasma and heart tissue. Prior administration of 2% squa-lene in feed for 45 days significantly reduced the isoprenaline-induced elevation in the levels of cholesterol, triglycerides, andfree fatty acids in plasma and heart tissue of rats following myocardial infarction. It exerted an anitlipidemic effect by re-ducing the level of low-density lipoprotein cholesterol with a parallel rise in the level of high-density lipoprotein cholesterolin plasma of experimental rats. A tendency to prevent the isoprenaline-induced depletion of phospholipids in the myocardiumof experimental rats was also observed. In the present study, the pretreatment with squalene significantly counteracted the iso-prenaline-induced lipid peroxidation and maintained the rats at near normal status. The results of the present study indicatethat the overall cardioprotective effect of squalene is probably related to an inhibition of lipid accumulation by its hypolipi-demic properties and/or its antioxidant properties.