Red light-emitting diode irradiation regulates oxidative stress and inflammation through SPHK1/NF-κB activation in human keratinocytes

Sun, Qiaochu,Kim, Hye-Eun,Cho, Hyejoung,Shi, Shuhan,Kim, Byungkuk,Kim, Okjoon Elsevier 2018 Journal of photochemistry and photobiology Biology Vol.186 No.-

<P><B>Abstract</B></P> <P>Oxidative stress, in which the amount of oxidants exceeds the capacity of antioxidant defense system, is a well-accepted pathogenesis of several human diseases. Light-emitting diode irradiation (LEDI) is an efficient strategy to counteract this condition. The biological effect of phototherapy, using visible light, has attracted recent attention especially in dermatological practice. However, little is known about the molecular mechanism of the anti-oxidant and anti-inflammatory effects of red light irradiation. We evaluated these effects of LEDI in HaCaT human keratinocyte cells under phorbol-12-myristate-13-acetate (PMA) induced reactive oxygen species (ROS). Microarray analysis revealed changes in 309 genes after LEDI. LEDI at 625 nm produced ROS scavenging and anti-inflammatory effects. One of the most important genes identified by microarray analysis was sphingosine kinase-1 (SPHK1), which is a key molecule in sphingolipid metabolism. SPHK1 knock-down drastically reduced ROS scavenging efficiency as well as expression levels of inflammation-related proteins in PMA-treated HaCaT cells. These results not only indicate the potential for the clinical application of 625-nm LEDI in treating skin disorders via ROS and/or inflammation, but also suggest SPHK1 as a potential therapeutic target in phototherapy.</P> <P><B>Highlights</B></P> <P> <UL> <LI> LEDI at 625 nm produced ROS scavenging and anti-inflammation. </LI> <LI> Sphingosine kinase-1 (SPHK1) was identified by microarray analysis in the experimental conditions. </LI> <LI> LEDI 625 nm has the potential for treating skin disorder via ROS and/or inflammation through SPHK1/NF-κB pathway. </LI> </UL> </P> <P><B>Graphical Abstract</B></P> <P>[DISPLAY OMISSION]</P>

치약개발 경향 분석 - 조성물에 관하여 -

황동구,나리수,조혜중,김서진,김혜은,SUNQIAOCHU,지형준,안규현,최홍란,김옥준 대한구강악안면병리학회 2016 대한구강악안면병리학회지 Vol.40 No.1

Along with toothbrushes, toothpaste has been used for a long time to improve the personal oral hygiene. Toothpaste was firstly introduced to Korea through as a form of powder by the Japanese company Lion Corporation in 1889 and in 1954, the LakHee Chemistry company released Korea’ s first ever tube type cream toothpaste called lucky toothpaste, and afterwards we got to where we are today in terms of toothpaste. As a variety of products are released and customer choices become more diverse, there needs to be a development of toothpaste that can fulfill customers’ needs and requests. Therefore, this study analyzes toothpastes and related patents submitted to the Patent Office from 1962 to June 2015, thereby examining the changes in toothpaste development trends up to the present day. This study was searched using the keyword ‘toothpaste’ in the patent, utility model search engine at Kipris(www.kipris.or.kr), a patent search site, and categorized a total of 2,465 patents, which excluded those rejected by the Patent Office and those unrelated to toothpaste, using titles, full texts of publications and announcements. Research results showed that in the past, from 1960 to 1989, patents related to toothpaste containers took up the highest ratio of 35%. However, after 1990, patents pertaining to toothpaste composites and functionality made up the highest percentage of 54%, and from 2010 to 2015, it occupied 72% of the whole; therefore, we analyzed in detail the patents related to composites and functionality. Taking a look at the objectives and effects of patents having to do with composites and functionality, we found out that from 1964 to June 2015, patents related to Base, a basic component of toothpaste, and those having to do with periodontal disease took up the highest percentages of 25% each, and from 2010~2015 in particular, patents concerning periodontal diseases were submitted 3% more than those about Base. Composites aiming to prevent and treat periodontal diseases can be divided into four different categories, based on components; among those, there were a total of 171 (57%) patents related to living organism and herbal medicine extracts, 107 (36%) patents using compounds, 16 (5%) patents using bamboo salt as well as salt, and 5 (2%) patents using mineral components such as gold, silver. Looking at these investigation results, it can be thought that with the increasing development of toothpaste related to periodontal diseases, there’ s a great amount of interest about toothpaste of effective periodontal disease prevention and treatment purposes. Along with this, we thought that research about how effective periodontal disease toothpastes actually are is also necessary.

좌측 경부 상방에 발생한 비전형 지방종선 종양

송두헌,엄찬용,임영관,김병국,김혜은,조혜중,SUNQIAOCHU,안규현,SHISHUHAN,김옥준 대한구강악안면병리학회 2017 대한구강악안면병리학회지 Vol.41 No.2

The occurrence of atypical lipomatous tumor of the head and neck is rare, and clinical and histologically differential diagnosis of the tumor is needed for other similar diseases. Herein we report atypical lipomatous tumor arising in neck area. And literature review was done. Complete excision with negative margins followed by long-term follow-up is recommended as the treatment of choice for these uncommon entities.

Application to Frequency Domain Optical Coherence Tomography (OCT) to Dental composite resin restored tooth Sang

전상미,이영희,지형준,조혜중,김서진,김혜은,SUNQIAOCHU,안규현,최홍란,김옥준 대한구강악안면병리학회 2016 대한구강악안면병리학회지 Vol.40 No.4

Composite resins are developed as restorative materials to improve esthetics and mechanical properties. To improve the physical properties of resin material, resin filler have to be added. However, no imaging method is adopted for resin filler distribution. Optical coherence tomography (OCT) is a optical imaging technique to delineate microscopic structures within biological tissue. The OCT application to dental composites resin and its filler is not described yet. So, this new and advanced optical method is needed for clinical application for evaluation of dental composite resin. To analyze the spatial distribution of dental composite resin and to evaluate the resin restoration in cavity, frequency domain optical coherence tomography (FD-OCT) was used for their analysis. Resin restored tooth was prepared. For morphological observation, serially sectioned teeth, conventional X-ray taking and micro computed-tomography (CT) images were compared with OCT images. The experiment has done to evaluate the success of the resin restoration using 3 dimensional structure OCT image. In this research, OCT is evaluated as a new technique to image resin restoration. The evaluation of resin restored tooth was performed by OCT. Inappropriate restoration such as marginal adaptation, large porosities, internal integrity and poor contour could be detected. Resin filler also could be checked by OCT. The distribution, number, regularity and size of resin filler can be differentiated from several commercial products. Considering the characteristics of the OCT, it can be used to evaluate the defects of resin restoration, resin filler distribution, and internal integrity between resin material and tooth structure. The OCT can be considered to be a new and advanced method for the evaluation of resin restorations.

구강캔디다증 환자의 항진균 치료

김해영,강재국,임영관,김병국,김혜은,조혜중,SUNQIAOCHU,안규현,SHISHUHAN,김옥준 대한구강악안면병리학회 2017 대한구강악안면병리학회지 Vol.41 No.2

The aim of this study was to examine the effects and the sensitivity of antifungal therapy for patients with oral candidiasis and to investigate the relationship among the signs & symptoms of patients and the ratio change of Candida species to antifungal therapy. Candida fungus culture test with ChromeIDTM Candida agar (CAN2) was carried out more than twice for 10 patients who visited Oral Medicine department of Chonnam National University Dental Hospital during the period from Dec. 2015 to Aug. 2016. After culturing the smear sample before and after antifungal therapy in ChromeIDTM Candida agar (CAN2), the number of colonies was counted to compare. Patients were divided into 5 group according to the therapeutic effects of the antifungal agents used: 1) high susceptibility to nystatin, 2) low susceptibility to nystatin, 3) high susceptibility to fluzonazole, 4) low susceptibility to fluzonazole, and 5) increased ratio of new Candida species. Although nystatin is used as first-line therapy in oral candidiasis, it is desirable to use fluconazole if patients had a history of the low sensitivity to nystatin or chemotherapy. Even if the patient's symptoms and signs are improved, there is a possibility of oral candidiasis recurring, so that clinicians should be careful during the treatment with antifungal agents.

구강질환을 가진 환자들의 캔디다균종의 분포와 채취 방법에 따른 배양 결과 비교

김윤규,강재국,임영관,김병국,김혜은,조혜중,SUNQIAOCHU,안규현,SHISHUHAN,김옥준 대한구강악안면병리학회 2017 대한구강악안면병리학회지 Vol.41 No.2

The purpose of this study is to analyze the distribution of Candida species in patients with oral disease and clarify the distinction of Candida culture test according to its isolation technique. 75 samples was isolated from 42 patients who visited Chonnam National University Dental Hospital due to oral disease from December 2015 to August 2016. For isolating the candida sampling, saliva sampling and oral swabbing were used. Acquired sampling was cultured in CHROMagar Candida Culture Medium, which indicates the candida species as color. Of the 42 patients, C. albicans was the most frequently isolated species in 39 patients. For 17 patients out of 21 who underwent saliva sampling and oral swabbing simultaneously, oral swabbing was quantitatively underestimated comparing to saliva sampling. 12 samples in 21 samples having particular Candida species were not isolated by oral swabbing. Considering the possibility of fungal infection in various oral disease, it is recommended to perform not only oral swabbing but saliva sampling when isolating Candida.