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Choue, Ryowon,Cho, Byung Hee Simon 생화학분자생물학회 1982 BMB Reports Vol.30 No.1
The ability of Hep-G2 cells to process [^(125)I]LDL under basal conditions was investigated. The receptor-binding and internalization of [^(125)I]LDL increased with the time of incubation in a saturable manner. After 4 h of incubation, 31.4 ng of [^(125)I]LDL was cell bound. The cells rapidly internalized [^(125)I]LDL via specific, receptor-mediated endocytosis. The amount of internalized [^(125)I]LDL reached a maximun of 96.7 ng at 2 h of incubation and remained constant for the next 2 h. The rate of degradation of internalized [^(125)I]LDL proceeded in a linear manner over the entire 4 h of incubation after an initial lag period. The effects of individial fatty acids (C18:0. C18:1, C18:2. and C18:3), differing in their degree of unsaturation. on the receptor-binding, internalization and degradation of [^(125)I]LDL were also investigated. Inclusion of 1.0 mM of each fatty acid into the culture medium significantly increased [^(125)I]LDL metabolism in Hep-G2 cells. Among the fatty acids tested, stearic acid had the least effect on the receptor-binding activity. There were no significant differences among the unsaturated fatty acids in LDL-receptor binding. The effect of individual fatty acids on the [^(125)I]LDL uptake was similar to that of the receptor-binding. showing a significantly lower effect with stearic acid. The amount of degraded material of internalized [^(125)I]LDL was the lowest with stearic acid when it was compared with unsaturated fatty acids.
( Jieun Kim ),( Ryowon Choue ),( Hyunjung Lim ) 한국임상영양학회 2016 Clinical Nutrition Research Vol.5 No.3
We aimed at assessing psychological variables and eating behaviors on quality of diet and life in South Korean women according to their weight status. Socio-psychology, eating behavior, quality of diet and quality of life data were assessed in 114 women (mean age: 34.5 ± 8.09 years). NEO Personality Inventory (NEO-PI-RS) and coping styles questionnaire were used to assess socio-psychology variables, and eating behavior was assessed using the Eating Attitudes Test-26 (EAT-26), Dutch Eating Behavior Questionnaire (DEBQ), and General Food Craving Questionnaire Test (G-FCQ-T). Quality of diet was analyzed by Diet Quality Index-international (DQI-I), and obesity-related quality of life was evaluated using the Korean Obesity-related Quality of life Scale (KOQOL). Significant differences were in the psychological variables and eating behaviors in the obese group than the normal and overweight groups (p < 0.05). The overall score of DQI-I was significantly lower in the obese group than that of their counterparts (p < 0.05). BMI was positively correlated with neuroticism, emotional eating, and obesity-related quality of life, and negatively correlated with diet quality. Neuroticism was positively correlated with emotional eating and food craving. Emotional eating was positively correlated with obesity-related quality of life. In conclusion, women with a higher BMI had significantly more problematic eating behaviors, poor diet quality and quality of life.