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          The Late Transcripts of SV40

          Roe,,Jung,Hye,Park,,Joo,Sang 한국유전학회 1988 Genes & Genomics Vol.10 No.4

          We have examined the structures and cellulardistributions of the SV40 late RNAs present in monkey cells at late times after infection. One particular RNA species, spliced at residue 373 (373-RNA), was found to be as abundant as the major late 16S RNAs. This result was unexpected since previous reports showed that the molecular ratio of the 373-spliced 19S RNA to 16S RNA is approximately 0.1 among either cytoplasmic polyadenylated or polysomal viral RNAs. Both sedimentation and electrophoretic analyses indicated that the 373-RNA was approximately 16S to 19S in size. Analysis of the structure of this novel RNA species by S1 mapping and primer extension showed that its 5′ end mapped to the major cap site at nucleotide residue 325 and that nucleotide residue 373 was joined covalently to nucleotide residue 558 as is the case with the majority of the cytoplasmic polyadenylated SV40 late 19S RNAs. However, whereas most SV40 late 16S RNA is polyadenylated and located in the cytoplasm, the majority of 373-RNA was found to lack polyA, exhibit sequence heterogeneity at its 3′ end and be located in the nucleus. The cellular distribution is reversed when the remaining 3′ splice region (around residue 1463) is deleted from this RNA. This implies that the 3' splice region, in the absence of its 5′ splice pair, inhibits the nuclear transport of this 19S spliced RNA, possibly by forming a splicing complex which is an intermediate for the formation of a complete spliceosome.

        • SCOPUSKCI등재

          The Characterization of an Abundant SV40 Late RNA Species in the Nucleus of the Infected Monkey Cell

          Roe,,Jung,Hye 한국유전학회 1986 Genes & Genomics Vol.8 No.4

          Late in the lytic cycle of infection of monkey cells SV40 produces two classes of late RNAs, 16S and 19S in size, which encode the virion proteins VP1 and VP2/3, respectively. These RNAs are made by differential splicing of the primary nuclear transcripts and exhibit heterogeneity in their 5'ends as well as splice sites utilized in processing. The mechanism of selection is not yet known. The relative abundances of the various species of spliced late RNAs present in the cytoplasm of SV40-infected monkey cells has been determined for both polysomal and polyadenylated RNA. The ratio of 16S to 19S cytoplasmic, polyadenylated RNA is approximately four. I have examined the structures and cellular distributions of the SV40 late RNAs present in monkey cells at late times after infection (48 hours after infection). One particular RNA species, spliced at residue 373 (373-RNA), was found to be as abundant as the major late 16S RNAs. This result was unexpected since previous reports showed that the molecular ratio of the 373-spliced 19S RNA to 16S RNA is approximately 0.1 among either cytoplasmic polyadenylated or polysomal viral RNAs. Both sedimentation and electrophoretic analyses indicated that the 373-RNA was approximately 16S to 19S in size. Therefore, it was not a splicing intermediate or the product of premature termination of transcription within the late leader region. Analysis of the structure of this RNA by S1 mapping and primer extension showed that its 5'end mapped to the major cap site (at nt. 325) and that nucleotide residue 373 was joined to residue 558 as is found in the majority of the cytoplasmic, polyadenylated SV40 late 19S RNAs. However, as judged by the oligo (dT)-cellulose chromatography, whereas most SV40 late 16S RNA is polyadenylated and located in the cytoplasm, the majority of 373-RNA was found to lack poly A tail, exhibit heterogeneity at its 3'end and be located in the nucleus. It is probable that the lack of poly A caused the preferential distribution of 373-RNA in the nucleus, thus regulating the amount of the functional mRNA in the cytoplasm. The structure of the 3'end of this RNA is currently under investigation. This will tell whether the 3'end is generated by transcriptional termination or by random degradation. Whether this RNA has ever been polyadenylated will be determined by the pulse-chase type of experiments.

        • SCOPUSKCI등재

          The Late Transcripts of SV40 Accumulated in The Nucleus of Infected Monkey Cells

          Roe,,Jung,Hye,Park,,Joo,Sang 한국유전학회 1988 Genes & Genomics Vol.10 No.4

          We have examined the structures and cellular distributions of the SV40 late RNAs present in monkey cells at late times after infection. One particular RNA species, spliced at residue 373 (373-RNA), was found to be as abundant as the major late 16S RNAs. This result was unexpected since previous reports showed that the molecular ratio of the 373-spliced 19S RNA to 16S RNA is approximately 0.1 among either cytoplasmic polyadenylated or polysomal viral RNAs. Both sedimentation and electrophoretic analyses indicated that the 373-RNA was approximately 16S to 19S in size. Analysis of the structure of this novel RNA species by S1 mapping and primer extension showed that its 5' end mapped to the major cap site at nucleotide residue 325 and that nucleotide residue 373 was joined covalently to nucleotide residue 558 as is the case with the majority of the cytoplasmic polyadenylated SV40 late 19S RNAs. However, whereas most SV40 late 16S RNA is polyadenylated and located in the cytoplasm, the majority of 373-RNA was found to lack polyA, exhibit sequence heterogeneity at its 3' end and be located in the nucleus. The cellular distribution is reversed when the remaining 3' splice region (around residue 1463) is deleted from this RNA. This implies that the 3' splice region, in the absence of its 5' splice pair, inhibits the nuclear transport of this 19S spliced RNA, possibly by forming a splicing complex which is an intermediate for the formation of a complete spliceosome.

        • KCI등재
        • KCI등재후보
        • SCOPUSKCI등재

          Biotin 표지 HBV DNA 를 이용한 Dot Hybridization 에 관한 연구

          노임환,이동후,노정혜 대한소화기학회 1988 대한소화기학회지 Vol.20 No.3

          A dot blot hybridization technique utilizing a biotin-labelled recombinant DNA probe was used to examine hepatitis B virus (HBV) DNA in serum. The lowest amount of HBV DNA in serum detectable by the color development of an avidin-biotin alkaline phosphatase complex was 40 picogram per 50 microliter. Validity of this method was confirmed by autoradiography using 32P-labelled and 3H-labelled HBV DNA probes. HBV DNA was found in 100% (34/34) of the HBsAg-positive and in 73.5% (25/34) of the HBsAg-negative subjects. In contrast, all nine cases showing negativity in HBV DNA were also HBsAg-negative. Correlation of Hbe antigen/antibody with HBV DNA was investigated in 19 sera of which HBsAg was negative but anti-HBc positive. Of 13 sera with anti-Hbe 10 (76.9%) cases revealed HBV DNA positivity, while four (66.7%) of six sera without anti-HBe were positive in HBV DNA. In conclusion, serum dot hybridization assay utilizing a birtinylated probe proved useful in the detection of a free form of HBV DNA regardless of the presence of HBsAg and irrespective of HBeAg-anti-HBe status. Also an anti-HBs does not always correspond to the clearance of HBV. And this indicates, in turn, that the potential infectivity of certain sera previously considered to be safe should be kept in mind. Moreover, it is emphasized that practical advantages in speed, reproducibility, and safety have made this alternative even more attractive than autoradiography using radioisotope-labelled probes.

        • KCI우수등재
        • KCI등재

          Memory and "Scraps of Truth" : Tim O`Brien`s Vietnam War Narratives 팀 오브라이언의 베트남전쟁 내러티브

          Roe,,Jae,H 한국현대영미소설학회 2001 현대영미소설 Vol.8 No.1

          본 논문은 베트남전쟁을 다룬Tim O'Brien의 작품들 - If I Die in a Combat Zone, Going After Cacciato, The Things They Carried - 에서 나타나는 베트남전쟁의 현실과 실험적인 서술방식을 논의한다. O'Brien의 작품들에는 내러티브의 형태와 베트남전쟁에 대한 작가의 시각이 불가분의 관계를 이루고있다. 전쟁당시 보병으로서의 경험을 재조명하는 과정에서, O'Brien은 미국인들이 잊어서는 안될 역사적인 교훈을 강조한다. Edward Herman과 Noam Chomsky는 베트남전쟁의 역사를 미국 제국주의와 민족학살의 역사로 설명한다. 또한, 이러한 역사가 미국매스미디어에 의해 의도적으로 변경되어서 미국인들이 베트남전쟁에 대해 잘못된 인식을 가지게 되었다고 주장한다. Jim Neilson은 이러한 잘못된 역사의식이 베트남전쟁문학에서도 나타난다고 주장한다. 이러한 현상은 문학작품들보다도 문학비평을 통해서 이루어졌다는 논지이다. 평론가와 학자들이 베트남전쟁 문학을 비역사적인, 미학적인 관점에서 분석해왔으며, 특히 역사적 현실을 불투명하게 만드는 postmodernism적인 이론에 의존하는 경향이 문제라고 지적한다. O'Brien의 작품들도 이러한 관점에서 분석되어왔는데, 본 논문은 이 작품들의 미학적, 문학이론적 측면보다는 역사적, 정치적 측면을 강조한다. 소설과 논픽션의 구분이 불투명한 O'Brien의 작품들에서 볼 수 있는 서술방식은 단지 문학적인 실험이 아니라 작가의 경험을 통해 베트남전쟁의 역사를 재조명하는 정치적인 의도가 있다는 것이 본 논문의 논지이다.

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