Basic phenotyping platform for genetically engineered mouse at KRIBB

Hae Rim Kim,Eun Kyoung Kim,Yong Sub Byun,Kang Ju Seong,Ju Young Lee,Yu Rim Kim,Min Young Lee,Sang Mi Cho,Taek Chang Lee,Hae Jin Lee,Young Ho Kho,Ok Seong Moon,Young Won Seo,Ki Hoon Lee,Hoyoung Ghang,W 한국실험동물학회 2015 한국실험동물학회 학술발표대회 논문집 Vol.2015 No.8

정보시스템 효과성 평가모형의 구축

임성택(Seong Taek Rim),한영춘(Young Choon Han) 한국데이타베이스학회 1996 한국데이타베이스학회 공동 학술대회 Vol.1996 No.11

Quantification of the impact of dumped manure waste from livestock farming to groundwater quality using stable N and O isotopes of <SUP>NO<SUB>3</SUB></SUP>- in conjunction with hydrochemical data

Seong-Jae Yoon,Kyoung-Ho Kim,Ho-Rim Kim,Tae-Seung Kim,Hyun-Goo Kim,Sen Hwa Park,Seong-Taek Yun 대한지질학회 2015 대한지질학회 학술대회 Vol.2015 No.10

가축 전염병 발생에 따른 소와 닭 사체의 화학적 처리 방법의 적용

Taek Geun Lee,오연수,Young-Seung Ko,Da-Yun Bae,Dong-Seob Tark,Chaekwang Rim,Ho-Seong Cho 한국동물위생학회 2022 韓國家畜衛生學會誌 Vol.45 No.2

In the event of an outbreak of a livestock epidemic, it has been considered that the existing burial-centered carcass disposal method should be improved ecofriendly for prevention of leachate and odors from burial basically in regard of pathogen inactivation. Therefore, the aim of this study is whether it was possible to treat the carcass of cattle and chickens using the chemical carcass treat-ment method. It was conducted to establish detailed treatment standards for the chemical treatment method of cattle and chicken carcasses based on the results of the proof of the absence of infectious diseases in cattle chickens. After inoculating cattle carcass with 10 pathogens (foot and mouth disease virus, bovine viral diarrhea virus, Mycobacterium bovis , Mycobacterium avium subsp. paratuber-culosis , Brucella abortus , Bacillus anthracis , Clostridium chauvoei , Clostridium perfringens , Esche-richia coli , and Salmonella Typhimurium) and chicken carcasses with low pathogenic avian influenza virus, Clostridium perfringens type C, E. coli and Salmonella Typhimurium, these were treated at 90°C for 5 hours in a potassium hydroxide liquid solution corresponding to 15% of the body weight. This method liquefies all cadaveric components and inactivates all inoculated pathogens by PCR and culture. Based on these results, it was possible to prove that chemical treatment of cattle and chicken carcasses is effective in killing pathogens and is a safe method without the risk of disease transmis-sion. The chemical treatment method of livestock carcasses can be suggested as an alternative to the current domestic burial-centered livestock carcass treatment method, preventing environmental pol-lution, and contributing to public health.

담낭관의 하부접합과 연관된 원발성 중복종양 1예

김성진,이진,강승식,박혜림,손진희,주상언,계세협,곽상택,김권수,정선화 대한소화기내시경학회 1998 Clinical Endoscopy Vol.18 No.4

Recently it has been discovered that anomalous union of pancreaticobiliary duct (AUPBD) may play an important role in the pathogenesis of bile duct cancer. Similar to this fact, there were a few reports on the clinical significance of the low junction of the cystic duct (LJCD). LJCD refers to the situation whereby the cystic duct enters the common duct at a low position between the upper margin of the pancreas and the duodenal opening of the bile duct. The pathogenetic mechanism in LJCD is similar to AUPBD in that the pancreatic juice refluxes to the bile duct, mixes with biles, and then generates the mutagens, which act as inflammatory substances and carcinogens on the epithelium of the bile duct. A 51-year-old male who experienced dyspepsia was hospitalized due to his abnormally functioning liver and dilated common bile duct. We confirmed primary cholangiocarcinoma on the common hepatic duct and gallbladder carcinoma associated with LJCD by ERCP and pathologic review of surgical specimen. We report a case of synchronous type double primary cancer associated with LJCD with a brief review of the literatures.

그레이브스병에서 치료에 따른 폐동맥압의 변화

남택만,조한수,이진서,송영림,김두만,두영철,박철영,정인경,홍은경,이성진,오기원,김현규,유재명,최문기,유형준,박성우 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.5

연구배경: 갑상선기능항진증에 의한 갑상선중독증 환자들은 호흡곤란을 호소하며, 그 원인으로 호흡근의 근력 약화, 좌심실부전으로 인한 폐모세혈관의 울혈, 기도저항의 증가, 갑상선종으로 인한 기관의 압박, 호흡기능 이상 등이 거론되고 있다. 폐동맥고혈압이 동반된 그레이브스병 환자가 여러 증례가 보고되었으며, 폐동맥고혈압 환자에게서 갑상선 자가항체와 갑상선기능저하증의 빈도가 높음이 알려지면서 갑상선중독증이 동반된 그레이브스병 환자에게서 관찰되는 호흡곤란의 한 원인으로 폐동맥압 증가가 작용할 가능성이 제시되고 있다. 이에 저자 등은 그레이브스병 환자를 대상으로 폐동맥압을 측정하고 치료 전후의 폐동맥압의 변화를 전향적으로 연구하였다. 방법: 갑상선중독증이 동반된 그레이브스병 환자와 정상 갑상선기능을 나타낸 대조군을 대상으로 갑상선기능검사 및 갑상선 자가항체를 측정하고, 심초음파를 이용하여 치료 전후로 폐동맥압을 측정하여 폐동맥압과 갑상선기능 및 갑상선 자가항체와의 관계, 폐동맥압의 변화 등을 관찰하였다. 결과: 1. 연구대상은 대조군 10명 (남녀비 1:9, 관해 상태의 그레이브스병 3명, 갑상선종 3명, 정상인 4명), 그레이브스병 환자 26명 (남녀비 7:19)이었다. 2. 대조군과 치료 전 그레이브스병 환자의 폐동맥압은 각각 23.5±2.32 mmHg, 29.6±10.3 mmHg이었고, 치료 전의 폐동맥압과 혈청 갑상선자극호르몬 결합억제 면역글로불린 (TBII) 농도는 유의한 양의 상관관계를 보였다. 3. 26명의 그레이브스병 환자 중에서 10명 (38.5%)이 폐동맥고혈압 (기준: 폐동맥압 > 30 mmHg)으로 진단되었다. 4. 치료 전후로 폐동맥압을 측정한 13명은 폐동맥압이 치료 전 29.6±10.3 mmHg에서 치료 후 폐동맥압 22.2±6.48 mmHg로 의미있게 감소하였다. 결론: 갑상선중독증을 보이는 그레이브스병 환자의 약 40% 정도에서 폐동맥고혈압이 발견되어 폐동맥고 혈압은 그레이브스병에 흔하게 동반하는 질환으로 생각된다. 향후 그레이브스병 환자에서 관찰되는 폐동맥압의 증가와 관련한 병인, 발생기전 및 임상적 의의 등에 대한 연구가 필요할 것으로 생각된다. Background: Exertional symptoms, dyspnea and impaired effort tolerance are common in patients with Graves' disease. Proposed explanations include: high-output left heart failure, ineffective oxygen utilization and respiratory muscle weakness. In addition, pulmonary hypertension has also been reported in patients with Graves' disease. A high prevalence of hypothyroidism and positive thyroid autoantibody were also observed in patients with pulmonary arterial hypertension. Therefore, the pulmonary artery pressure in patients with Graves' disease was evaluated. Methods: Two-dimensional and Doppler echocardiographic examinations (Hewlett Packard Sonos 2500) were performed to determine the pulmonary artery (PA) pressure in 26 Graves' disease patients, both before and after treatment (23 patients with propylthiouracil and 3 with RAI), and in 10 euthyroid controls. The changes in the PA pressure after treatment were evaluated in 13 patients with Graves' disease, who became euthyroid after treatment. Results: The pulmonary artery pressure was increased in the untreated Graves' disease patients compared to the normal controls (23.5±2.32 vs. 29.6±10.3 mmHg). 38.5% of the Graves' disease patients (10/26) showed pulmonary arterial hypertension (PA>30 mmHg) and the serum TBII level was higher in the Graves' disease patients with pulmonary arterial hypertension than in those with normal PA pressure (P<0.05). In the Graves' patients who became euthyroid after treatment, the PA pressure was significantly decreased. Conclusion: 38.5% of the untreated Graves' disease patients showed pulmonary arterial hypertension, and the pulmonary artery pressure was significantly decreased in those who became euthyroid after treatment. The pathogenesis and clinical importance of pulmonary arterial hypertension in Graves' disease requires further studies (J Kor Soc Endocrinol 18:465∼472, 2003).

A Phenotypic Characterization of a Mutant Strain with Yellow Belly Derived from C57BL/6J Mice

Hae Rim Kim(김해림),Eun-Kyoung Kim,Yong Sub Byun,Hye Min Choi,Ju Seong Kang,Taek-Chang Lee,Young-Won Seo,Won-Kee Yoon,Hyoung-Chin Kim,Ki-Hoan Nam 한국실험동물학회 2014 한국실험동물학회 학술발표대회 논문집 Vol.2014 No.2

지하수 내 질소 오염원 판별을 위한 질산염 동위원소 및 수화학 자료의 단계별 분류

윤성재(Seong-Jae Yoon),윤성택(Seong-Taek Yun),김경호(Kyung-Ho Kim),김호림(Ho-Rim Kim) 대한지질학회 2014 대한지질학회 학술대회 Vol.2014 No.10

Assessment of nitrogen application limits in agro-livestock farming areas using quantile regression between nitrogen loadings and groundwater nitrate levels

Kim, Ho-Rim,Yu, Soonyoung,Oh, Junseop,Kim, Kyoung-Ho,Oh, Yun-Yeong,Kim, Hyun Koo,Park, Sunhwa,Yun, Seong-Taek Elsevier 2019 Agriculture, ecosystems & environment Vol.286 No.-

<P><B>Abstract</B></P> <P>The excessive application of nitrogen in agro-livestock farming areas has led to serious groundwater contamination around the world; thus, the efficient control of N loads is crucial to manage nitrate contamination of groundwater. In this study, to suggest an optimal N application limit as a key guideline of N management, we examine the impact of anthropogenic N loading on nitrate levels of shallow groundwater, using a large dataset (<I>n =</I> 4,000) collected in 2012–2014 from 100 agro-livestock farming districts in South Korea. Not considering the time lag and legacy problem, quantile regression is performed to overall assess the relationship between nitrate contamination and anthropogenic N input across the full range of conditional distribution of groundwater nitrate concentrations because of heteroscedasticity. As a result, positive gradients (<I>β<SUB>τ</SUB> </I>) meaning the rates of changes are found between groundwater nitrate concentrations and land-derived N loads at all quantiles; <I>β<SUB>τ</SUB> </I> increases as the quantile is higher and is as large as 36.48 ± 10.73 mg NO<SUB>3</SUB> <SUP>−</SUP>/L per land-derived N input (on a log scale) at the 90% quantile. The quantile map indicates that the high gradient (i.e., the large sensitivity to N loading) is related to agricultural land use, low elevation, and low topographic slopes. In fact, groundwater nitrate concentrations increase as the percentage of agricultural land increases but the percentage of forest, elevation and slope angle decreases, which suggests that land use, elevation, and slope should be considered when attempting to evaluate N application limits. This study suggests the N limits below 170 kg ha<SUP>-1</SUP> year<SUP>-1</SUP> based on the drinking water standard (44.3 mg/L as NO<SUB>3</SUB> <SUP>−</SUP>) at the quantiles higher than 70% which mostly occur in flat agricultural lowland areas. Our method used in this study can be applied elsewhere to establish efficient management practices for groundwater nitrate contamination, considering local environmental factors.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An empirical relationship between N inputs and groundwater nitrate levels over S. Korea. </LI> <LI> Results of quantile regression is useful to suggest optimal N application limits. </LI> <LI> Nitrate contamination is more sensitive at higher quantiles toward low-lying lands. </LI> <LI> N application limit below 170 ha<SUP>−1</SUP> year<SUP>-1</SUP> is suggested for typical agricultural areas. </LI> </UL> </P>

제주도 지하수 질산염 농도의 시·공간적 변화 특성: 장기(1993-2015) 모니터링 자료의 평가

김호림(Ho-Rim Kim),오준섭(Junseop Oh),도현권(Hyun-Kwon Do),이경진(Kyung-Jin Lee),현익현(Ik-Hyun Hyun),오상실(Sang-Sil Oh),감상규(Sang-Kyu Kam),윤성택(Seong-Taek Yun) 대한자원환경지질학회 2018 자원환경지질 Vol.51 No.1

1993년부터 2015년까지 관측된 제주도 지하수 장기모니터링 관측정(N = 4,835)에서 수집된 지하수 수질자료(N = 21,568)를 기반으로 질산성질소의 시공간적 변동 특성을 평가하였다. 제주도 지하수의 질산성질소 농도의 중앙값은 2.5 mg/L로서 다른 국가나 대륙의 조사 결과에 비해 다소 높거나 유사한 것으로 나타났다. 또한 지하수 용도, 행정구역 및 고도 별로 유의한 차이를 보였다. 특히, 산간 지역에 비해 저지대 해안가에 위치한 농업 및 주거지역에서 농도가 높음을 확인하였다. Mann-Kendall 및 Sen’s slope 분석을 활용한 질산성질소 농도의 추세 분석 결과, 하류 저지 대에 비해 중산간지역에서의 질산성질소 농도 증가 경향이 뚜렷하였다. 제주도 내 토지 피복의 시계열 변화 특성과 결부 지어 보면, 중산간지역의 오염 증가 추세는 농업지역의 확장 등 인위적 활동 증가에 기인한 결과로 판단된다. 반면,기지정된 지하수자원특별관리구역에서는 전반적으로 질산성질소 농도의 감소 경향이 나타났는데, 이는 지하수 관리 측면에서 수질관리를 위한 적극적인 정책이 유효함을 시사한다. 본 연구에서는 제주도 지하수의 질산성질소 오염관리를 위한 적정 방안을 제안한다. The spatio-temporal variations of nitrate concentrations in groundwater of Jeju Island were evaluated by an analysis of time series groundwater quality data (N = 21,568) that were collected from regional groundwater monitoring (number of wells = 4,835) for up to 20 years between 1993 and 2015. The median concentration of NO 3 -N is 2.5 mg/L, which is slightly higher than those reported from regional surveys in other countries. Nitrate concentrations of groundwater in wells tend to significantly vary according to different water usage (of the well), administrative districts, and topographic elevations: nitrate level is higher in low-lying agricultural and residential areas than those in high mountainous areas. The Mann-Kendall trend test and Sen’s slope analysis show that nitrate concentration in mid-mountainous areas tends to increase, possibly due to the expansion of agricultural areas toward highland. On the other hand, nitrate concentrations in the Specially Designated Groundwater Quality Protection Zones show the temporally decreasing trend, which implies the efficiency of groundwater management actions in Jeju. Proper measures for sustainable groundwater quality management are suggested in this study.