Holstein 초유 중 Lactoferrin, IgA, IgG₁, IgG₂ 정량과 미생물의 성장에 미치는 영향

랜친핸드,배형철,남명수,Renchinthand, Gereltuya,Bae, Hyoung-Churl,Nam, Myoung-Soo 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.4

본 실험은 홀스타인 젖소의 초유에 함유된 lactoferrin, IgA, $IgG_1,\;IgG_2$의 함량과 병원성미생물의 항균효과, 유산균증식효과를 알아보기 위하여 수행하였다. 홀스타인종 젖소 초유의 lactoferrin, IgA, $IgG_1,\;IgG_2$의 함량은 여름철의 경우 분만 후 첫째 날에 각각 0.30 mg/mL, 0.37 mg/mL, 4.00 mg/mL, 0.37 mg/mL, 겨울철의 경우 분만 후 첫째 날에 각각 1.16 mg/mL, 2.60 mg/mL, 13.35 mg/mL, 1.30 mg/mL로 측정되었다. 무열처리한 초유와 $65^{\circ}C$에서 30분간 열처리한 초유에서 배양한 병원성 미생물의 변화는 E. coli 균주에 대하여 초유의 항균활성이 나타났다. 초유 첫째 날, 둘째 날, 세째 날의 유산균수 성장촉진 효과는 B. longum, L. acidophilus S. themophilus의 상업용 혼합균주와 L. acidophilus, L. casei, L. bulgaricus, L. lactis subsp. cremoris에서 모두 정상유보다 균수가 증가하였다. 초유 첫째 날, 둘째 날, 세째 날의 pH는 배양 15시간 후 B. longum, L. acidophilus S. themophilus의 상업용 혼합균주와 L. acidophilus, L. casei, L. bulgaricus, L. lactis subsp. cremoris에서 각각 4.97-5.22 및 5.96-6.47로 나타났으나, 정상유에서는 각각 4.89, 6.50-6.81이었다. 초유 첫째 날, 둘째 날, 세째 날의 산도는 B. longum, L. acidophilus S. themophilus의 상업용 혼합균주와 L. acidophilus, L. casei, L. bulgaricus, L. lactis subsp. cremoris에서 각각 0.75-0.88% 및 0.29-0.48%로 나타났으나, 정상유에서는 각각 0.70%, 0.20-0.25% 이었다. This experiment was carried out to measure the content of lactoferrin, IgA, $IgG_1,\;IgG_2$, in Holstein colostrum, and to test the effect of it's colostrum on the antibacterial activity to pathogenic bacteria and the growth stimulation of lactic acid bacteria. Colostrum was collected at the first, second, and third day after parturition in summer and winter season. The levels of lactoferrin, IgA, $IgG_1,\;and\;IgG_2$ in Holstein cow colostrum were 0.30 mg/mL, 0.37 mg/mL, 4.00 mg/mL, 0.37 mg/mL, respectively, on the first day of the summer season whereas they were 1.16 mg/mL, 2.60 mg/mL, 13.35 mg/mL, 1.30 mg/mL on the first day of the winter season, postpartum. Heat treated ($65^{\circ}C$ for 30 min) or non-treated colostrum showed antibacterial activity toward Escherichia coli. The growth of commercial mixed strains (Bifidobacterium longum, Lactobacillus acidophilus, Streptococcus themophilus), L. acidophilus, L. casei, L. bulgaricus, and L. lactis subsp. cremoris were improved in first, second and third day colostrum compared to normal milk. Commercial miked strains (B. longum, L. acidophilus S. themophilus) lowered the pH to 4.97-5.22 and 4.89 while increasing the titratable acidity to 0.75-0.88% and 0.70% in colostrum and normal milk, respectively. However, L. casei, L. bulgaricus, L. lactis subsp. cremoris lowered the pH to 5.96-6.47 and 6.5-6.8 while increasing the titratable acidity to 0.29-0.48% and 0.20-0.25% in colostrum and normal milk, respectively.

국내산 초유의 현황과 이용에 관한 연구

배형철,랜친핸드,나석한,최성현,남명수,Bae, Hyung-Churl,Renchinthand, Gereltuya,Na, Seuk-Han,Choi, Seong-Hyun,Nam, Myoung-Soo 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.4

Questionnaire on utilization of colostrum was carried out in 33 farms. Highest milking cow numbers were 20-40 heads in 45.5% of the farms. Calf numbers were 20-40 heads in 39.4% of the dairy farms and 40-60 heads in 42.4% of the farms. The amount of colostrum production per day was 20-30 kg in 30.3% of the farms and 30-40 kg in 30.3% of the farms. Colostrum intake per calf per day was 4 kg in 45.5% farms. Surplus colostrum after intake by calf was farms (85%) was wasted in 67% of the farms. More than 90% of dairy farms recognize an necessity to use surplus colostrum. Selling price of surplus colostrum was below 1,000 won per kg at 15 dairy farms and 1,000-2,000 won per kg at 4 dairy farms. Colostrum collection and use should be done by the dairy farms of antibiotics free. Quality control of colostrum should be done by testing antibiotics, microbacterium, chemical composition, somatic cells and etc. The colostrum is subjected to cool down below $4^{\circ}C$ just after milking and process for the colostrum products.

발효초유사료 급여가 자돈의 성장에 미치는 영향

나석한,최성현,랜친핸드,배형철,남명수,Na, Seuk-Han,Choi, Seong-Hyun,Renchinthand, Gereltuya,Bae, Hyoung-Churl,Nam, Myoung-Soo 한국축산식품학회 2008 한국축산식품학회지 Vol.28 No.3

This study was carried out to assess the fermentation properties of lactic acid bacteria (LAB) isolated from bovine colostrum and effects of feeding fermented colostrum feed on the growth to piglet. A total of 427 colonies were isolated from bovine colostrum on the BCP plate count agar. These LAB isolated were subcultured in 10% reconstituted skim milk, and seven strain thereof were selected for their highest acid productions, and two strain thereof were finally selected for their excellent sugar utilization. These strains were identified as Streptococcus thermophilus and Streptococcus macedonicus based on l6S rDNA sequencing data, named S. thermophilus CNB-11 and S. macedonicus CNB-11 respectively. For fermentation profiles, sugar utilization, acid production and viable cell counts were excellent in S. thermophilus CNB-11 as compared with S. macedoniclts CNB-11 after 48 hour. The effect of feeding fermented colostrum feed 0.5% using S. thermophilus CNB-11 was investigated for growth rate, analysis of blood and incidence of diarrhea. 24 heads of piglets were divided into two groups: the experimental and the control of 12 animals each. The average growth rate in the pigs fed fermented colostrum feed was higher 16.73% compared with control diet (p<0.05). There were no differences in the concentrations of blood glucose, cholesterol, albumin and globulin in pigs fed fermented colostrum feed as compared with control piglets. Incidence of diarrhea was no in pigs fed fermented colostrum feed as compared with control piglets.

항원감작식품과 어린이 알레르기 질환과의 관계

문은경,배형철,남명수,Moon, Eun-Kyoung,Bae, Hyung-Churl,Renchinthand, Gereltuya,Nam, Myoung-Soo 한국축산식품학회 2007 한국축산식품학회지 Vol.27 No.3

2005년 3월부터 2006년 4월까지 어린이 74명(남 47, 여 27, 1-4세)을 대상으로 식품에 대한 IgE 특이 항체량을 검사하였다. 알레르기 증상을 나타낸 어린이의 연령은 영 유아(0-3세)가 34명, 4-6세가 24명, 7-9세가 8명, 10세 이상이 8명으로, 나이가 어릴수록 알레르기 증상을 보이는 어린이가 많은 것으로 나타났다. 검사한 어린이가 21종류의 식품 중 3종류, 4종류, 5종류의 식품에 반응을 보인 어린이는 각각 10명, 21명, 15명으로 나타났다. IgE 항체반응에서 class 2 미만으로 나타난 어린이의 식품은 콩 28명, 우유 29명, 치즈 21명, 난백 7명, 돼지고기 18명으로 나타났고 class 2 이상은 콩 20명, 우유 17명, 치즈 24명, 난백 20명, 돼지고기 21명으로 나타나 이러한 식품들이 주로 식품 알레르기와 관련이 있는 것으로 판단된다. 가족력에서는 알레르기 증상을 나타낸 비율이 남아는 45.5%, 여아는 50%로 절반 정도로 나타났고, 가족 중 알레르기 질병의 병력은 아토피 피부염은 26%, 아토피 비염은 10.5%,아토피 피부염과 아토피 비염은 31.5%, 두드러기 21.0%, 천식 10.5%이었다. 섭식력은 모유와 분유를 혼합하여 섭취한 어린이가 분유만 섭취한 어린이, 모유만 섭취한 어린이보다 높았다. 알레르기 유발식품으로 제한하는 식품은 계란과 우유 12.5%, 우유 2.5%, 계란 10.0%등이었다. We have investigated the relationship between food allergen sensitization and allergic disease in 74 child (male 47, female 27) patients from 0 to 14 years of age diagnosed with allergic disease. The age distribution for the study was: newborn to 3 years old, 34 children; 4 to 6 years old, 24 children; 7 to 9 years old, 8 children and above 10 years old, 8 children. Of the 74 children, 10 children were allergic to 3 of the 21 types of foods tested, 21 children were allergic to 4 types and 15 children were allergic to 5 types. The results of specific IgE tests for class 2 (0.070-3.49 IV/mL, IgE density in serum) showed that 29 children were allergic to milk, 28 children to bean, 21 children to cheese, 7 children to egg, and 18 children to pork, while over class 2, 20 children were allergic to bean, 17 children to milk, 24 children to cheese, 20 children to egg, and 21 children to pork. A questionnaire was used to survey family allergy history and diet patterns for 40 child (male 22, female 18) patients with allergic disease. The frequencies of a family history of allergy were 45.5% for males and 50.0% for females. The allergic diseases included atopic dermatitis: 26.0%, atopic nasitis: 10.5%, atopic dermatitis + atopic nasitis : 31.5%, hives: 21.0%, and asthma: 10.5%. Children on diets of mixed breast feeding and infant formula were more allergic than those on either breast feeding or infant formula feeding. Eliminated allergenic foods were egg + milk: 12.5%, egg: 10.0%, and milk 2.5%.