소아치과의 새로운 비전 - 우식 없는 건강한 치아를 보존하기 위해: 소아치과에서의 CAMBRA 사용

Ramos-Gomez, Francisco,Kim, Young Jae,Ng, Man-Wai,Yang, Stephanie 대한소아치과학회 2013 大韓小兒齒科學會誌 Vol.40 No.1

최근 2세에서 5세 사이 소아의 우식 발생율이 상당히 증가하고 있다. 유아기우식증(Early Childhood Caries)은 향후 영구치의 우식 발생 가능성에 대한 예측인자이며 사회 저소득층과 다문화 가정 자녀에서 특히 발생빈도가 높은데, 이는 충분히 예방과 관리가 가능하다. 만1세때부터 정기적으로 치과를 방문하고 이 때 우식위험도를 체계적으로 평가하여 환자들에게 맞춤형 예방 전략을 제시할 수 있기 때문이다. 유아기우식증의 예방을 위한 CAMBRA (Caries Management by Risk Assessment)에 대해 가장 최신의 개념과 사용법을 소개하고자 한다. 소아치과의사는 CAMBRA를 통해 개개인의 위험인자를 분석하여 연령에 따른 적절한 예방법을 수립하고 향후 우식 발생시 치료방향의 지침을 정하게 된다. Early childhood caries prevalence has increased significantly in children ages 2~5 years. ECC disproportionately affects lower socioeconomic and minority groups, is a predictor for future decay, but is preventable and manageable. Caries risk assessment systematically derives a patient's caries risk and is important during an infant oral health visit beginning at age 1. Information obtained through a risk assessment can guide a disease management care path tailored to an individual's age and risk to effectively treat and manage one's caries disease process.

Effects of Processing Conditions on the Protein Quality of Fried Anchovy Kamaboko Engraulis japonica

Ramos, Leny R. Ordonez,Choi, Nam-Do,Ryu, Hong-Soo The Korean Society of Fisheries and Aquatic Scienc 2012 Fisheries and Aquatic Sciences Vol.15 No.4

The effects of processing and frozen storage conditions on the quality of anchovy Engraulis japonica fried surimi gels were investigated. Protein content decreased after surimi gel processing from 19.6% (raw meat) to 12.1% (kamaboko) due to the added ingredients and change in water content. Lipid content decreased from 2.8% (raw meat) to 1.3% in minced and 0.5% in surimi, but fried kamaboko showed a 6.9 % lipid level. Thiobarbituric acid values and thiobarbituric acid reactive substances levels were highest in kamaboko samples, 89.5 and 1.9 mg/g solid, and increased gradually with storage time to 101.8 and 4.6 mg/g solid, respectively. In vitro protein digestibility increased from 79.2% in raw anchovy to 88.5% in kamaboko samples. Levels of trypsin inhibitor decreased gradually with processing and during storage time from 2.43 in raw anchovy to 0.31 mg/g solid in the kamaboko sample after 60 days of frozen storage. No noticeable changes in total essential amino acid was observed during processing conditions. Computed protein efficiency ratio for kamaboko was highest (2.59) compared with whole anchovy (1.96), minced (1.94) and surimi (2.50). Fresh fried anchovy kamaboko showed similar values of hardness, springiness, gumminess and chewiness to commercial surimi gel, but a higher values were seen for fracturability and adhesiveness, and lower values for cohesiveness and resilience. The frozen and thawed anchovy kamaboko showed higher values for all of these rheological parameters compared with fresh and commercial kamaboko. Anchovy kamaboko showed the lowest lightness (62.9) and redness (0.16) and similar yellowness (11.9) compared with commercial kamaboko. Frozen storage and vacuum packaging were effective maintaining the shelf life of anchovy kamaboko within 30 days, but were not effective after 45 days due to fat oxidation.

Overexpression and secretion of AgaA7 from Pseudoalteromonas hodoensis sp. nov in Bacillus subtilis for the depolymerization of agarose

Ramos, K.R.M.,Valdehuesa, K.N.G.,Cabulong, R.B.,Moron, L.S.,Nisola, G.M.,Hong, S.K.,Lee, W.K.,Chung, W.J. IPC Science and Technology Press ; Elsevier Scienc 2016 Enzyme and microbial technology Vol.90 No.-

<P>Interest in agar or agarose-based pharmaceutical products has driven the search for potent agarolytic enzymes. An extracellular beta-agarase (AgaA7) recently isolated from Pseudoalteromonas hodoensis sp. nov was expressed in Bacillus subtilis, which was chosen due to its capability to overproduce and secrete functional enzymes. Phenotypic analysis showed that the engineered B. subtilis secreted a functional AgaA7 when fused with the aprE signal peptide (SP) at the amino-terminus. The maximum agarolytic activity was observed during the late logarithmic phase. To further improve the secretion of AgaA7, an expression library of AgaA7 fused to different naturally occurring B. subtilis SPs was created. The amount of AgaA7 secreted by the clones was compared through activity assay, immuno-blot, and purification via affinity chromatography. Although the aprE SP can readily facilitate the secretion of AgaA7, other SPs such as yggA, pel, and lipA were relatively more efficient. Among these SPs, lipA was the most efficient in improving the secretion of AgaA7.The use of B. subtilis as host for the expression and secretion of agarolytic and other hydrolytic enzymes can be a useful tool in the field of white biotechnology. (C) 2016 Elsevier Inc. All rights reserved.</P>

Identification and characterization of a thermostable endolytic β-agarase Aga2 from a newly isolated marine agarolytic bacteria <i>Cellulophaga omnivescoria</i> W5C

Ramos, Kristine Rose M.,Valdehuesa, Kris Niñ,o G.,Nisola, Grace M.,Lee, Won-Keun,Chung, Wook-Jin Elsevier 2018 New biotechnology Vol.40 No.2

<P><B>Abstract</B></P> <P>Research on the enzymatic breakdown of seaweed-derived agar has recently gained attention due to the progress in green technologies for marine biomass utilization. The enzymes known as agarases catalyze the cleavage of glycosidic bonds within the polysaccharide. In this study, a new β-agarase, Aga2, was identified from <I>Cellulophaga omnivescoria</I> W5C. Aga2 is one of four putative agarases from the W5C genome, and it belongs to the glycoside hydrolase 16 family. It was shown to be exclusive to the <I>Cellulophaga</I> genus. Agarase activity assays showed that Aga2 is an endolytic-type β-agarase that produces tetrameric and hexameric neoagaro-oligosaccharides, with optimum activity at 45°C and pH 8.0. Zinc ions slightly enhanced its activity while manganese ions had inhibitory effects even at very low concentrations. Aga2 has a K<SUB>m</SUB> of 2.59mgmL<SUP>−1</SUP> and V<SUB>max</SUB> of 275.48Umg<SUP>−1</SUP>. The K<SUB>cat</SUB> is 1.73×10<SUP>2</SUP> s<SUP>−1</SUP>, while the K<SUB>cat</SUB>/K<SUB>m</SUB> is 8.04×10<SUP>6</SUP> s<SUP>−1</SUP> M<SUP>−1</SUP>. Aga2 also showed good thermostability at 45°C and above, and retained >90% of its activity after repeated freeze-thaw cycles. Bioinformatic analysis of its amino acid sequence revealed that intrinsic properties of the protein (e.g. presence of certain dipeptides and the relative volume occupied by aliphatic amino acids) and tertiary structural elements (e.g. presence of salt bridges, hydrophobic interactions and H-bonding) contributed to its thermostability.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Endolytic β-agarase Aga2 was identified from <I>Cellulophaga omnivescoria</I> W5C. </LI> <LI> Aga2 optimum activity was at 45°C, pH 8.0, with NA4 and NA6 as major products. </LI> <LI> Aga2 was thermostable at high temperatures as well as after many freeze-thaw cycles. </LI> <LI> Intrinsic properties and tertiary structural elements confer the thermostability. </LI> </UL> </P>

The Philippine Experience : Its Challenges and Contribution to the Pacific Region

Ramos-Shahani, Leticia 仁荷大學校 國際關係硏究所 1986 The Pacific Century Vol.1986 No.-

Neuro-Fuzzy Modelling and Stable PD Controller for Angular Position in Steering Systems

Ramos-Fernández Julio C.,López-Morales Virgilio,Márquez-Vera Marco A.,Pérez Juan Manual Xicotencatl,Suarez-Cansino Joel 한국자동차공학회 2021 International journal of automotive technology Vol.22 No.6

The precision agriculture and soil tillage are tasks which can be achieved by Automated Tractors (AT) through the integration of several servomechanisms. In order to reach a high autonomy under various work conditions of the AT, control laws’ design and tuning are paramount. The aim of this work is to develop an angle position controller for steering systems applied to an AT. We introduce an automatic electronic steering system by using a fuzzy model, obtained through an Adaptive Neuro-Fuzzy Inference System (ANFIS) algorithm training. A proportional derivative (PD) controller is also tuned through a Takagi-Sugeno fuzzy model (T-S). Furthermore, several closed-loop stability tests were carried out, in numerical simulation and real-time implementation. The feasibility of our methodology is illustrated through the tracking of several angles’ profiles in a real test scenario. For the tracking of a trajectory or several set-points, we have obtained mean errors about 0.6545 and 0.8651 degrees, respectively. Some mechatronic integrations to convert a conventional tractor into a basic Autonomous Agriculture Off-road Tractor (AAOT) are also shown.

Overexpression and characterization of a novel α-neoagarobiose hydrolase and its application in the production of D-galactonate from <i>Gelidium amansii</i>

Ramos, Kristine Rose M.,Valdehuesa, Kris Niñ,o G.,Maza, Perry Ayn Mayson M.,Nisola, Grace M.,Lee, Won-Keun,Chung, Wook-Jin Elsevier Science B.V., Amsterdam. 2017 PROCESS BIOCHEMISTRY Vol.63 No.-

<P><B>Abstract</B></P> <P>An α-neoagarobiose hydrolase (α-NABH) from <I>Cellulophaga</I> sp. W5C, designated as AhgI, was identified, purified, and characterized. Its 1227 base pairs of coded sequence translate into a 408-amino acid protein that belongs to the GH117 family. Multiple sequence alignment of AhgI with other known α-NABHs showed 83% homology with AhgA from <I>Zobellia galactanivorans</I>. AhgI had an apparent molecular weight of 45kDa and was highly active at pH 7.0 and 20°C. The <I>K</I> <SUB>m</SUB> and <I>V</I> <SUB>max</SUB> values for neoagarobiose (NA2) were 1.03mM and 10.22U/mg, respectively. Apart from NA2, the enzyme showed activity against other neoagaro-oligosaccharides such as neoagarotetraose (NA4) and neoagarohexaose (NA6). AhgI was then employed in a prototype process to produce D-galactonate from <I>Gelidium amansii</I>. Agar from <I>G. amansii</I> was hydrothermally extracted and then enzymatically hydrolyzed by sequential addition of β-agarases and AhgI. The final hydrolysate containing D-galactose was then utilized for the microbial production of D-galactonate. This is believed to be the first report on the identification and characterization of an α-NABH derived from <I>Cellulophaga</I> species and its subsequent application in the synthesis of a value-added chemical directly from marine macroalgae.</P> <P><B>Highlights</B></P> <P> <UL> <LI> α-Neoagarobiose hydrolase was identified from <I>Cellulophaga</I> sp. W5C. </LI> <LI> Optimum conditions for enzyme activity are pH 7.0 and 20°C. </LI> <LI> AhgI was active towards neoagarobiose, neoagarotetraose, and neoagarohexaose. </LI> <LI> AhgI was applied for the hydrolysis of agar and subsequent D-galactonate production. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

Guide for Current Nutrigenetic, Nutrigenomic, and Nutriepigenetic Approaches for Precision Nutrition Involving the Prevention and Management of Chronic Diseases Associated with Obesity

Ramos-Lopez, Omar,Milagro, Fermí,n I.,Allayee, Hooman,Chmurzynska, Agata,Choi, Myung Sook,Curi, Rui,De Caterina, Raffaele,Ferguson, Lynnette R.,Goni, Leticia,Kang, Jing X.,Kohlmeier, Martin,Mart S. Karger AG 2017 Journal of nutrigenetics and nutrigenomics Vol.10 No.1

<P>Chronic diseases, including obesity, are major causes of morbidity and mortality in most countries. The adverse impacts of obesity and associated comorbidities on health remain a major concern due to the lack of effective interventions for prevention and management. Precision nutrition is an emerging therapeutic approach that takes into account an individual's genetic and epigenetic information, as well as age, gender, or particular physiopathological status. Advances in genomic sciences are contributing to a better understanding of the role of genetic variants and epigenetic signatures as well as gene expression patterns in the development of diverse chronic conditions, and how they may modify therapeutic responses. This knowledge has led to the search for genetic and epigenetic biomarkers to predict the risk of developing chronic diseases and personalizing their prevention and treatment. Additionally, original nutritional interventions based on nutrients and bioactive dietary compounds that can modify epigenetic marks and gene expression have been implemented. Although caution must be exercised, these scientific insights are paving the way for the design of innovative strategies for the control of chronic diseases accompanying obesity. This document provides a number of examples of the huge potential of understanding nutrigenetic, nutrigenomic, and nutriepigenetic roles in precision nutrition.</P>

UNCONVENTIONAL DYNAMIC EFFECTS IN PLASTIC WATER PIPE SYSTEMS

RAMOS HELENA,COVAS DIDIA,ALMEIDA A. B. DE 한국수자원학회 2005 한국수자원학회 학술대회 Vol.2005 No.1

Formation of ghost images due to metal objects on the surface of the patient's face: A pictorial essay

Ramos, Barbara Couto,da Silva Izar, Bruna Raquel,Pereira, Jessica Lourdes Costa,Souza, Priscilla Sena,Valerio, Claudia Scigliano,Tuji, Fabricio Mesquita,Manzi, Flavio Ricardo Korean Academy of Oral and Maxillofacial Radiology 2016 Imaging Science in Dentistry Vol.46 No.1

Panoramic radiographs are a relatively simple technique that is commonly used in all dental specialties. In panoramic radiographs, in addition to the formation of real images of metal objects, ghost images may also form, and these ghost images can hinder an accurate diagnosis and interfere with the accuracy of radiology reports. Dentists must understand the formation of these images in order to avoid making incorrect radiographic diagnoses. Therefore, the present study sought to present a study of the formation of panoramic radiograph ghost images caused by metal objects in the head and neck region of a dry skull, as well as to report a clinical case in order to warn dentists about ghost images and to raise awareness thereof. An understanding of the principles of the formation of ghost images in panoramic radiographs helps prevent incorrect diagnoses.