http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
( Ramachandran priyadharshini ),( Ngoc Phuong Thao Nguyen ),( Joon Ho Choi ),( Yun Chan Kang ),( Marimuthu Jeya ),( Jung Kul Lee ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.3
A high β-glucosidase (BGL)-producing strain, Stereum hirsutum, was identified and isolated and showed a maximum BGL activity (10.4 U/ml) when cultured with Avicel and tryptone as the carbon and nitrogen sources, respectively. In comparison with other BGLs, BGL obtained from S. hirsutum showed a higher level of activity to cellobiose (Vmax = 172 U/mg, and kcat = 281/s). Under the optimum conditions (600 rpm, 30oC, and pH 6.0), the maximum BGL activity of 10.4 U/ml with the overall productivity of 74.5 U/l/h was observed. BGL production was scaled up from a laboratory scale (7-L fermenter) to a pilot scale (70-L fermenter). When S. hirsutum was cultured in fed-batch culture with rice straw as the carbon source in a 70-L fermenter, a comparable productivity of 78.6 U/l/h was obtained. Furthermore, S. hirsutum showed high levels of activity of other lignocellulases (cellobiohydrolase, endoglucanase, xylanase, and laccase) that are involved in the saccharification of biomasses. Application of S. hirsutum lignocellulases in the hydrolysis of Pinus densiflora and Catalpa ovata showed saccharification yields of 49.7% and 43.0%, respectively, which were higher than the yield obtained using commercial enzymes.
Ramachandran, Esakky,John, Suchit Ashish,Abraham, Gerard,Bhattacharya, Partha Sarathi 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.4
The PTM3 (MADS-box) gene characterized from flower of Aspen was ectopically expressed in cotton and was found to result in desirable agronomic traits. Among several transgenic lines, the PTM3 cotton event-10 was found to flower significantly earlier than the control and yield better [Ramachandran et al. (2011)]. We report our findings on performance based on greenhouse evaluation and inheritance of PTM3 cotton event-10. The $T_1$ progeny of event-10 were grown in pots under RBD design. The progeny were confirmed by kanamycin imbibition test, PCR, and GUS assay which showed the segregation of transgene at about 3:1 ratio. GUS assay was performed with pollen from all transgenic plants; plants that expressed gus in all pollen versus those that showed segregation for expression were found to be at a ratio of 1:2. Similar to observations made in the $T_0$ generation of event-10, the agronomic evaluation of $T_1$ progeny exhibited, on an average, earliness of 13 days in flowering, 13.5 days in crop maturity, and 22% of yield enhancement. The $T_2$ progeny of homozygous lines were grown on field soil in the greenhouse under strip trial design to see the effect as observed from potted plants of $T_1$ progeny. Inheritance of transgene cassette to $T_2$ progeny was confirmed by the same tests used to analyze $T_1$ progeny. As exhibited by $T_0$ and $T_1$ progenies, the $T_2$ progeny also showed earliness of 8 days in flowering, 12 days in crop maturity, and 17% of yield enhancement. The data generated from the progeny over two generations confirms that the PTM3 cotton event-10 is superior in agronomic characters as compared to non-transgenic cotton and is of interest for breeding shorter duration varieties with improved yield.
Esakky Ramachandran,Suchit Ashish John,Gerard Abraham,Partha Sarathi Bhattacharya 한국작물학회 2011 Journal of crop science and biotechnology Vol.14 No.4
The PTM3 (MADS-box) gene characterized from flower of Aspen was ectopically expressed in cotton and was found to result in desirable agronomic traits. Among several transgenic lines, the PTM3 cotton event-10 was found to flower significantly earlier than the control and yield better [Ramachandran et al. (2011)]. We report our findings on performance based on greenhouse evaluation and inheritance of PTM3 cotton event-10. The T1 progeny of event-10 were grown in pots under RBD design. The progeny were confirmed by kanamycin imbibition test, PCR, and GUS assay which showed the segregation of transgene at about 3:1 ratio. GUS assay was performed with pollen from all transgenic plants; plants that expressed gus in all pollen versus those that showed segregation for expression were found to be at a ratio of 1:2. Similar to observations made in the T0 generation of event-10, the agronomic evaluation of T1 progeny exhibited, on an average, earliness of 13 days in flowering, 13.5 days in crop maturity, and 22% of yield enhancement. The T2 progeny of homozygous lines were grown on field soil in the greenhouse under strip trial design to see the effect as observed from potted plants of T1 progeny. Inheritance of transgene cassette to T2 progeny was confirmed by the same tests used to analyze T1progeny. As exhibited by T0 and T1 progenies, the T2 progeny also showed earliness of 8 days in flowering, 12 days in crop maturity,and 17% of yield enhancement. The data generated from the progeny over two generations confirms that the PTM3 cotton event-10is superior in agronomic characters as compared to non-transgenic cotton and is of interest for breeding shorter duration varieties with improved yield.
Ramachandran, K.,Zahoor, A.,Raj Kumar, T.,Nahm, K.S.,Balasubramani, A.,Gnana Kumar, G. Korean Society of Industrial and Engineering Chemi 2017 Journal of industrial and engineering chemistry Vol.46 No.-
<P>The tetragonal alpha-phase structured manganese dioxide nanorods were densely grown over the nitrogen doped graphite nanofibers (NGNF/MnO2) via a facile and one-pot hydrothermal technique. The amperometric results depicted that NGNF/MnO2 composite exhibited a high sensitivity of 1096 mu A mM(-1) cm(-2) and a wide linear range from 0.1 to 11 mM with the lower detection limit of 1.25 mu M toward the non-enzymatic hydrogen peroxide (H2O2) detection, owing to the synergistic effects of NGNF and MnO2. In the prospect of excellent selectivity, real sample analysis and other striking advantages, the developed non-enzymatic sensors hold promising potential for the tight monitoring of H2O(2) in the food and clinical diagnosis fields. (C) 2016 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.</P>
Synthesis, Structural Characterization, and Catalytic Activity of Flower Like ZnO Nanostructures
Ramachandran, K.,Kumar, G. Gnana,Kim, Ae Rhan,Yoo, Dong Jin Korean Chemical Society 2014 Bulletin of the Korean Chemical Society Vol.35 No.4
Tageteserecta flower like zinc oxide nanostructures composed of hexagonal nanorods were synthesized via sonochemical method at room temperature. The synthesized nanomaterials exhibited wurtzite hexagonal phase structure with the single crystalline nature. The diameter of the individual nanorods that constitute the flower shaped zinc oxide structures is in the range of 120-160 nm. The sonication time effectively determined the morphological properties of the prepared materials. The catalytic activity of prepared zinc oxide nanostructures towards N-formylation reactions were evaluated without any surface modification and the nanostructures exhibited good reaction yield with the prompt recyclability behavior.
Ramachandran Anirudh,Montenegro Michelle,Singh Maninder,Dixon Trevor,Kayani Waqas,Liang Timothy,Yu Nick,Reddy Srinivas,Liveris Anna,Manyapu Mallika,McEwan Alyssia A.,Nguyen Vincent T.,Sonenthal Necham 대한응급의학회 2022 Clinical and Experimental Emergency Medicine Vol.9 No.3
Objective Hip fractures are associated with significant morbidity and mortality. Ultrasound-guided peripheral nerve blocks are a safe method to manage pain and decrease opioid usage. The pericapsular nerve group (PENG) block is a novel, potentially superior block because of its motor-sparing effects. Through training, simulation, and supervision, we aim to determine whether it is feasible to perform the PENG block in the emergency department. Methods Phase 1 consisted of emergency physicians attending a workshop to demonstrate ultrasound proficiency, anatomical understanding, and procedural competency using a low-fidelity model. Phase 2 consisted of a prospective, observational, feasibility study of 10 patients with hip fractures. Pain scores, side effects, and opioid usage data were collected. Results The median pain score at time 0 (time of block) was 9 (interquartile range [IQR], 6.5–9). The median pain score at 30 minutes was 4 (IQR, 2.0–6.8) and 3.5 (IQR, 1.0–4.8) at 4 hours. All 10 patients required narcotics prior to the initiation of the PENG block with a median dosage of 6.25 morphine milligram equivalents (MME; IQR, 4.25–7.38 MME). After the PENG block, only 30% of the patients required further narcotics with a median dosage of 0 MME (IQR, 0–0.6 MME) until operative fixation. Conclusion In this feasibility study, PENG blocks were safely administered by trained emergency physicians under supervision. We demonstrated data suggesting a trend of pain relief and decreased opiate requirements, and further investigation is necessary to measure efficacy.
The Chicken Aorta as a Simulation-Training Model for Microvascular Surgery Training
Ramachandran, Savitha,Chui, Christopher Hoe-Kong,Tan, Bien-Keem Korean Society of Plastic and Reconstructive Surge 2013 Archives of Plastic Surgery Vol.40 No.4
As a technically demanding skill, microsurgery is taught in the lab, in the form of a course of variable length (depending on the centre). Microsurgical training courses usually use a mixture of non-living and live animal simulation models. In the literature, a plethora of microsurgical training models have been described, ranging from low to high fidelity models. Given the high costs associated with live animal models, cheaper alternatives are coming into vogue. In this paper we describe the use of the chicken aorta as a simple and cost effective low fidelity microsurgical simulation model for training.
Chiral flat bands: Existence, engineering, and stability
Ramachandran, Ajith,Andreanov, Alexei,Flach, Sergej American Physical Society 2017 Physical Review B Vol.96 No.16
<P>We study flat bands in bipartite tight-binding networks with discrete translational invariance. Chiral flat bands with chiral symmetry eigenenergy E = 0 and host compact localized eigenstates for finite range hopping. For a bipartite network with a majority sublattice chiral flat bands emerge. We present a simple generating principle of chiral flat-band networks and as a showcase add to the previously observed cases a number of new potentially realizable chiral flat bands in various lattice dimensions. Chiral symmetry respecting network perturbations-including disorder and synthetic magnetic fields-preserve both the flat band and the modified compact localized states. Chiral flat bands are spectrally protected by gaps and pseudogaps in the presence of disorder due to Griffiths effects.</P>
A Honey-Hive based Efficient Data Aggregation in Wireless Sensor Networks
Ramachandran, Nandhakumar,Perumal, Varalakshmi The Korean Institute of Electrical Engineers 2018 Journal of Electrical Engineering & Technology Vol.13 No.2
The advent of Wireless Sensor Networks (WSN) has led to their use in numerous applications. Sensors are autonomous in nature and are constrained by limited resources. Designing an autonomous topology with criteria for economic and energy conservation is considered a major goal in WSN. The proposed honey-hive clustering consumes minimum energy and resources with minimal transmission delay compared to the existing approaches. The honey-hive approach consists of two phases. The first phase is an Intra-Cluster Min-Max Discrepancy (ICMMD) analysis, which is based on the local honey-hive data gathering technique and the second phase is Inter-Cluster Frequency Matching (ICFM), which is based on the global optimal data aggregation. The proposed data aggregation mechanism increases the optimal connectivity range of the sensor node to a considerable degree for inter-cluster and intra-cluster coverage with an improved optimal energy conservation.