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      • KCI등재

        Molecular cloning and expression analysis of a myosin light chain 1 (MLC‐1) gene from Indian meal moth Plodia interpunctella (Lepidoptera: Pyralidae)

        Qiu‐Ning Liu,Dan-Dan Bian,Chun-Lin Zhou,Bo-Ping Tang 한국곤충학회 2015 Entomological Research Vol.45 No.6

        Myosin light chain 1 (MLC‐1) protein acts in the organization, dynamics and transport processes associated with the cytoskeleton. In this work, an MLC‐1 gene was cloned and characterized from the Indian meal moth, Plodia interpunctella (Lepidoptera: Pyralidae). The isolated PiMLC‐1 cDNA is 913 bp, including a 5′‐untranslated region (UTR) of 79 bp, 3′‐UTR of 381 bp and an open reading frame (ORF) of 453 bp encoding a polypeptide of 150 amino acids, which contains two calcium binding domains (EF‐hands). The deduced PiMLC‐1 protein sequence has 39–94% comparison with other individuals. The qPCR analysis revealed that PiMLC‐1 was expressed in the four developmental stages (egg, larva, pupa and adult) and in all tissues tested, suggesting that it plays an important role in development of P. interpunctella. Based on the MLC‐1 amino acids, phylogenetic analysis showed a similar topology with the traditional classification, suggesting the potential value of the MLC‐1 protein in phylogenetic inference.

      • KCI등재

        『春秋左氏傳』所程規的人文精神

        沈秋雄 연세대학교 인문과학연구소 1999 人文科學 Vol.80 No.-

        以上通過春秋時代鬼神與人二者地位之升降, 以及當時各國賢士大夫對於天道陰陽一流事件的詮釋二端, 藉以說明『春秋左氏傳』一書所呈現的人文精神. 全書中類似的事例還有不少, 本文只是聯發其端緖, 其他的例子就不再擧了. 而要說明『春秋左氏傳』一書所呈顯的人文精神, 除了本文所提示的二端以外, 當然還可以從其他方面觀察, 全面論述, 且俟諸異日. 至於於晩近學者所重視的人與自然之間的和諧對應開題. 由於不在本文的範圍之內, 這裡也就略去不談了.

      • SCIESCOPUSKCI등재

        PCR Identification and Phylogenetic Analysis of Trichomonas gallinae from Domestic Pigeons in Guangzhou, China

        Qiu, Shen-Ben,Lv, Meng-Na,He, Xi,Weng, Ya-Biao,Zou, Shang-Shu,Wang, Xin-Qiu,Lin, Rui-Qing The Korean Society for Parasitology 2017 The Korean Journal of Parasitology Vol.55 No.3

        Avian trichomoniasis caused by Trichomonas gallinae is a serious protozoan disease worldwide. The domestic pigeon (Columba livia domestica) is the main host for T. gallinae and plays an important role in the spread of the disease. Based on the internal transcribed spacers of nuclear ribosomal DNA of this parasite, a pair of primers (TgF2/TgR2) was designed and used to develop a PCR assay for the diagnosis of T. gallinae infection in domestic pigeons. This approach allowed the identification of T. gallinae, and no amplicons were produced when using DNA from other common avian pathogens. The minimum amount of DNA detectable by the specific PCR assay developed in this study was 15 pg. Clinical samples from Guangzhou, China, were examined using this PCR assay and a standard microscopy method, and their molecular characteristics were determined by phylogenetic analysis. All of the T. gallinae-positive samples detected by microscopic examination were also detected as positive by the PCR assay. Most of the samples identified as negative by microscopic examination were detected as T. gallinae positive by the PCR assay and were confirmed by sequencing. The positive samples of T. gallinae collected from Guangzhou, China, were identified as T. gallinae genotype B by sequencing and phylogenetic analyses, providing relevant data for studying the ecology and population genetic structures of trichomonads and for the prevention and control of the diseases they cause.

      • An Increase in Synaptic NMDA Receptors in the Insular Cortex Contributes to Neuropathic Pain

        Qiu, Shuang,Chen, Tao,Koga, Kohei,Guo, Yan-yan,Xu, Hui,Song, Qian,Wang, Jie-jie,Descalzi, Giannina,Kaang, Bong-Kiun,Luo, Jian-hong,Zhuo, Min,Zhao, Ming-gao AAAS 2013 Science signaling Vol.6 No.275

        <P><B>Stopping the Pain</B></P><P>Damage to the central or peripheral nervous system can trigger the development of neuropathic pain, which can manifest as painful sensations in response to stimuli that are not normally painful. Qiu <I>et al.</I> found that mice that had developed neuropathic pain after peripheral nerve injury showed changes in synaptic plasticity and increased abundance of synaptic NMDA receptors in the insular cortex, a region of the brain that is activated by acute and chronic pain. Using pharmacological inhibitors and transgenic mice, they mimicked these changes in vitro with insular cortical slices and thus identified the signaling pathway responsible. Mice injected with NMDA receptor inhibitors showed reduced behavioral signs of neuropathic pain after peripheral nerve injury. Thus, blocking NMDA receptor function in the insular cortex may prevent the development of neuropathic pain.</P>

      • Sensitivity of Gastric Cancer Cells to Chemotherapy Drugs in Elderly Patients and Its Correlation with Cyclooxygenase-2 Expression

        Qiu, Zhen-Qin,Qiu, Zhen-Rong Asian Pacific Organization for Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.8

        Objective: To explore the sensitivity of gastric cancer cells to chemotherapy drugs in elderly patients and its correlation with cyclooxygenase-2 (COX-2) expression in cancer tissue. Materials and Methods: Forty-three elderly patients with gastric cancer (observation group) and 31 young patients with gastrointestinal tumors (control group) who were all diagnosed by pathology and underwent surgery in the 89th Hospital of Chinese People's Liberation Army were selected. Drug sensitivity testing of tumor cells in primary culture was carried out in both groups using a methyl thiazolyl tetrazolium (MTT) method, and the expression of COX-2 and the factors related to multi-drug resistance (MDR) in cancer tissue were assessed by immunohistochemistry. Results: The inhibition rates (IR) of vincristine (VCR), 5-fluorouracil (5-FU), oxaliplatin (L-OHP), mitomycin (MMC) and epirubicin (eADM) on tumor cells in the observation group were dramatically lower than in the control group, with statistical significance (P<0.05 or P<0.01). The positive rates of COX-2, glutathione s-transferase-${\pi}$ (GST-${\pi}$) and P glycoprotein (P-gp) expression in cancer tissue in the observation group were all higher than in control group (P<0.05), while that of DNA topoisomerase $II{\alpha}$ ($TopoII{\alpha}$) expression lower than in the control group (P<0.01). In the observation group, COX-2 expression in cancer tissue had a significantly-positive correlation with GST-${\pi}$ and P-gp (r=0.855, P=0.000; r=0.240, P=0.026), but a negative correlation with $TopoII{\alpha}$ (r=-0.328, P=0.002). In the control group, COX-2 expression in cancer tissue was only correlated with P-gp positively (r=0.320, P=0.011). Bivariate correlation analysis displayed that COX-2 expression in cancer tissue in the observation group had a significantly-negative correlation with the IRs of 5-FU, L-OHP, paclitaxel (PTX) and eADM in tumor cells (r=-0.723, P=0.000; r=-0.570, P=0.000; r=-0.919, P=0.000; r=-0.781, P=0.000), but with hydroxycamptothecine (HCPT), VCR and 5-FU in the control group (r=-0.915, P=0.000; r=-0.890, P=0.000; r=-0.949, P=0.000). Conclusions: Gastric cancer cells in elderly patients feature stronger MDR, which may be related to high COX-2 expression.

      • SCIESCOPUSKCI등재

        An Improved Lightweight Two-Factor Authentication and Key Agreement Protocol with Dynamic Identity Based on Elliptic Curve Cryptography

        Qiu, Shuming,Xu, Guosheng,Ahmad, Haseeb,Xu, Guoai,Qiu, Xinping,Xu, Hong Korean Society for Internet Information 2019 KSII Transactions on Internet and Information Syst Vol.13 No.2

        With the rapid development of the Internet of Things, the problem of privacy protection has been paid great attention. Recently, Nikooghadam et al. pointed out that Kumari et al.'s protocol can neither resist off-line guessing attack nor preserve user anonymity. Moreover, the authors also proposed an authentication supportive session initial protocol, claiming to resist various vulnerability attacks. Unfortunately, this paper proves that the authentication protocols of Kumari et al. and Nikooghadam et al. have neither the ability to preserve perfect forward secrecy nor the ability to resist key-compromise impersonation attack. In order to remedy such flaws in their protocols, we design a lightweight authentication protocol using elliptic curve cryptography. By way of informal security analysis, it is shown that the proposed protocol can both resist a variety of attacks and provide more security. Afterward, it is also proved that the protocol is resistant against active and passive attacks under Dolev-Yao model by means of Burrows-Abadi-Needham logic (BAN-Logic), and fulfills mutual authentication using Automated Validation of Internet Security Protocols and Applications (AVISPA) software. Subsequently, we compare the protocol with the related scheme in terms of computational complexity and security. The comparative analytics witness that the proposed protocol is more suitable for practical application scenarios.

      • KCI등재

        Preparation and characterization of the antimony telluride hexagonal nanoplates

        Qiu-li Yuan,Qiu-lin Nie,De-xuan Huo 한국물리학회 2009 Current Applied Physics Vol.9 No.1

        Nanostructured thermoelectric materials are of much interest for improving the thermoelectric figure of merit. In the present work, Sb2Te3 hexagonal nanoplates were successfully fabricated via a facile hydrothermal method. The products were characterized by means of X-ray diffraction, field-emission scanning electron microscope, transmission electron microscope and energy-dispersive X-ray spectroscopy. The results show that the typical Sb2Te3 crystal is hexagonal in shape with about 35 nm in thickness and 300 nm in edge length. The mechanism for formation of Sb2Te3 hexagonal nanoplates is primarily discussed. Nanostructured thermoelectric materials are of much interest for improving the thermoelectric figure of merit. In the present work, Sb2Te3 hexagonal nanoplates were successfully fabricated via a facile hydrothermal method. The products were characterized by means of X-ray diffraction, field-emission scanning electron microscope, transmission electron microscope and energy-dispersive X-ray spectroscopy. The results show that the typical Sb2Te3 crystal is hexagonal in shape with about 35 nm in thickness and 300 nm in edge length. The mechanism for formation of Sb2Te3 hexagonal nanoplates is primarily discussed.

      • SCIESCOPUSKCI등재

        A review on thermohydraulic and mechanical-physical properties of SiC, FeCrAl and Ti<sub>3</sub>SiC<sub>2</sub> for ATF cladding

        Qiu, Bowen,Wang, Jun,Deng, Yangbin,Wang, Mingjun,Wu, Yingwei,Qiu, S.Z. Korean Nuclear Society 2020 Nuclear Engineering and Technology Vol.52 No.1

        At present, the Department of Energy (DOE) in Unite State are directing the efforts of developing accident tolerant fuel (ATF) technology. As the first barrier of nuclear fuel system, the material selection of fuel rod cladding for ATFs is a basic but very significant issue for the development of this concept. The advanced cladding is attractive for providing much stronger oxidation resistance and better in-pile behavior under sever accident conditions (such as SBO, LOCA) for giving more coping time and, of course, at least an equivalent performance under normal condition. In recent years, many researches on in-plie or out-pile physical properties of some suggested cladding materials have been conducted to solve this material selection problem. Base on published literatures, this paper introduced relevant research backgrounds, objectives, research institutions and their progresses on several main potential claddings include triplex SiC, FeCrAl and MAX phase material Ti3SiC2. The physical properties of these claddings for their application in ATF area are also reviewed in thermohydraulic and mechanical view for better understanding and simulating the behaviors of these new claddings. While most of important data are available from publications, there are still many relevant properties are lacking for the evaluations.

      • KCI등재

        Density functional theory and experimental studies of Cu2+ activation on a cyanide-leached sphalerite surface

        Tingsheng Qiu,Yuanqing He,Xianhui Qiu,Xiuli Yang 한국공업화학회 2017 Journal of Industrial and Engineering Chemistry Vol.45 No.-

        Density functional theory calculations,flotation behavior, X-ray photoelectron spectroscopy, and infraredspectra were combined to reveal the Cu2+ activation mechanism on a cyanide (CN )-leached sphaleritesurface. Low CN adsorption enhanced the activation capability of Cu2+ on the sphalerite surface,whereas the hydrophilicity of the sphalerite increased with increasing CN coverage, which hindered Cu2+ adsorption. As the amount of chemically adsorbed Cu2+ on the sphalerite surface increased, Cu2+ beganto desorb CN , leading to the same result, which is consistent with the low CN coverage.

      • KCI등재

        An Improved Lightweight Two-Factor Authentication and Key Agreement Protocol with Dynamic Identity Based on Elliptic Curve Cryptography

        Shuming Qiu,Guosheng Xu,Haseeb Ahmad,Guoai Xu,Xinping Qiu,Hong Xu 한국인터넷정보학회 2019 KSII Transactions on Internet and Information Syst Vol.13 No.2

        With the rapid development of the Internet of Things, the problem of privacy protection has been paid great attention. Recently, Nikooghadam et al. pointed out that Kumari et al.’s protocol can neither resist off-line guessing attack nor preserve user anonymity. Moreover, the authors also proposed an authentication supportive session initial protocol, claiming to resist various vulnerability attacks. Unfortunately, this paper proves that the authentication protocols of Kumari et al. and Nikooghadam et al. have neither the ability to preserve perfect forward secrecy nor the ability to resist key-compromise impersonation attack. In order to remedy such flaws in their protocols, we design a lightweight authentication protocol using elliptic curve cryptography. By way of informal security analysis, it is shown that the proposed protocol can both resist a variety of attacks and provide more security. Afterward, it is also proved that the protocol is resistant against active and passive attacks under Dolev-Yao model by means of Burrows-Abadi-Needham logic (BAN-Logic), and fulfills mutual authentication using Automated Validation of Internet Security Protocols and Applications (AVISPA) software. Subsequently, we compare the protocol with the related scheme in terms of computational complexity and security. The comparative analytics witness that the proposed protocol is more suitable for practical application scenarios.

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