SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis

Mao Jianxin,Wang Di,Wang Dong,Wu Qi,Shang Qiliang,Gao Chu,Wang Huanbo,Wang Han,Du Mu,Peng Pandi,Jia Haoruo,Xu Xiaolong,Wang Jie,Yang Liu,Luo Zhuojing 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-

Mitochondrial dysfunction plays a major role in the development of intervertebral disc degeneration (IDD). Sirtuin 5 (SIRT5) participates in the maintenance of mitochondrial homeostasis through its desuccinylase activity. However, it is still unclear whether succinylation or SIRT5 is involved in the impairment of mitochondria and development of IDD induced by excessive mechanical stress. Our 4D label-free quantitative proteomic results showed decreased expression of the desuccinylase SIRT5 in rat nucleus pulposus (NP) tissues under mechanical loading. Overexpression of Sirt5 effectively alleviated, whereas knockdown of Sirt5 aggravated, the apoptosis and dysfunction of NP cells under mechanical stress, consistent with the more severe IDD phenotype of Sirt5 KO mice than wild-type mice that underwent lumbar spine instability (LSI) surgery. Moreover, immunoprecipitation-coupled mass spectrometry (IP-MS) results suggested that AIFM1 was a downstream target of SIRT5, which was verified by a Co-IP assay. We further demonstrated that reduced SIRT5 expression resulted in the increased succinylation of AIFM1, which in turn abolished the interaction between AIFM1 and CHCHD4 and thus led to the reduced electron transfer chain (ETC) complex subunits in NP cells. Reduced ETC complex subunits resulted in mitochondrial dysfunction and the subsequent occurrence of IDD under mechanical stress. Finally, we validated the efficacy of treatments targeting disrupted mitochondrial protein importation by upregulating SIRT5 expression or methylene blue (MB) administration in the compression-induced rat IDD model. In conclusion, our study provides new insights into the occurrence and development of IDD and offers promising therapeutic approaches for IDD.

Removal of trichloroethylene DNAPL trapped in porous media using nanoscale zerovalent iron and bimetallic nanoparticles: Direct observation and quantification

Wang, Qiliang,Jeong, Seung-Woo,Choi, Heechul Elsevier 2012 Journal of hazardous materials Vol.213 No.-

<P><B>Highlights</B></P><P>► TCE DNAPL removal inside pores using NZVI or bimetals in a 2-D system was visualized. ► Presence of nitrate and humic substances decrease the TCE DNAPL removal efficiency. ► Presence of ethanol increases the TCE DNAPL removal efficiency. ► Metal catalysts enhance the TCE DNAPL removal using NZVI in a short term reaction. ► Metal catalysts do not increase the DNAPL removal efficiency for a long term reaction.</P> <P><B>Abstract</B></P><P>Direct trichloroethylene (TCE) dense non-aqueous phase liquid (DNAPL) removal inside pore areas using nanoscale zerovalent iron (NZVI) and bimetallic nanoparticles were first investigated in a water-saturated porous glass micromodel. Effects of nitrate, aqueous ethanol co-solvent, humic substance, and elapsed time on TCE DNAPL removal using NZVI were studied by direct visualization. The removal efficiency was then quantified by directly measuring the remaining TCE DNAPL blobs area using an image analyzer. As ethanol content of co-solvent increased, TCE DNAPL removal by NZVI was also increased implying sequential TCE DNAPL removal mechanisms: as dissolved TCE was degraded by NZVI, TCE dissolution from TCE blobs would be then facilitated and the TCE blob areas would be eventually reduced. The presence of nitrate and humic substance hindered the NZVI reactivity for the TCE DNAPL removal. In contrast, the TCE DNAPL removal efficiency was enhanced using bimetallic nanoparticles in a short-term reaction by generating atomic hydrogen for catalytic hydro-dechlorination. However, all TCE DNAPL removal efficiencies reached the same level after long-term reaction using both NZVI and bimetallic nanoparticles. Direct TCE DNAPL observation clearly implied that TCE blobs existed for long time even though all TCE blobs were fully exposed to NZVI and bimetallic nanoparticles.</P>

Fabrication and Characteristics of TiO2 Nanotubes on Hemispherical Diamond Films Using ZnO Nanorods as Template

Qiliang Wang,Qi Yu,Junsong Liu,Taotao Ai,Hongdong Li 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2015 NANO Vol.10 No.6

Zinc oxide (ZnO) nanorods (NRs) have been synthesized as a template to fabricate TiO2 nanotubes (TiNTs) on hemispherical diamond film by liquid-phase deposition (LPD) method. The process concerning the formation of TiNTs was analyzed. Based on the results of XRD and Raman spectroscopy, it is demonstrated that TiNTs was successfully obtained after removing ZnO NRs by chemical etching at room temperature. The TiNTs on hemispherical diamond film show higher performance photocatalysis, examined by the degradation of the reactive yellow 15(RY15) solution. The corresponding mechanism is discussed.

Controllable synthesis, characterization, and magnetic properties of nanoscale zerovalent iron with specific high Brunauer-Emmett-Teller surface area

Wang, Qiliang,Kanel, Sushil Raj,Park, Hosik,Ryu, Anna,Choi, Heechul Springer-Verlag 2009 JOURNAL OF NANOPARTICLE RESEARCH Vol.11 No.3

Tomboy: New Womanhood, Stardom, and Chinese Cinema in the 1930s

Wangli HUANG,Qiliang HE 성균관대학교 동아시아학술원 2020 Sungkyun Journal of East Asian Studies Vol.20 No.2

This article explores Tomboy (Huashen guniang 化身姑娘, 1936), arguably one of the most viewed films in China in the mid-1930s, and Yuan Meiyun’s 袁美雲 (1917–1999) stardom in prewar Shanghai. Tomboy’s popularity was a testimony to the rise of female stardom and female fandom in this decade. The 1930s also saw both the final triumph of the rally for women’s rights since the May Fourth period and the Nationalist Party’s pro-family, anti-woman backlash. Tomboy catapulted Yuan Meiyun to superstardom in the context of the cacophonous debates on new femininity in China in the relatively conservative mid-1930s. This article thus argues that because Yuan Meiyun exemplified a new type of woman who was modern, attractive, and independent but exempted herself from modern life’s vices and hedonism, her eclectic womanhood both on the screen and in the media narrative gained widespread acclaim and thereby contributed to her success as a film star.

Measurement of unsteady transition on a pitching airfoil using dynamic pressure sensors

Yongwei Gao,Qiliang Zhu,Long Wang 대한기계학회 2016 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.30 No.10

Unsteady boundary layer transition on a pitching OA209 airfoil in a wind tunnel was detected by using pressure fluctuation measurement at different oscillation frequency. Thirty Kulite dynamic pressure transducers flush-mounted on the airfoil surface recorded pressure signatures, and root mean square of pressure signatures were calculated. Results indicated that the criterion of transition for static airfoil defined as the peak of root mean square of pressure fluctuation was still suitable for detection of transition on a pitching airfoil. Fixed transition experiment for pitching airfoil was performed to validate the conclusion. Effect of oscillation frequency on transition was investigated. For small reduced frequency, the hysteresis loop is larger near leading edge. With increasing in the oscillation frequencies, the transition was promoted and relaminarization was enhanced.

Sorption and desorption kinetics of PAHs in coastal sediment

신원식,오상화,Qiliang Wang,송동익 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.1

Sorption and desorption kinetics of PAHs (naphthalene, phenanthrene and pyrene) in coastal sediment were investigated. Several kinetic models were used to analyze the kinetics: one-site mass transfer model (OSMTM),pseudo-first-order kinetic model (PFOKM), pseudo-second-order kinetic model (PSOKM), two compartment firstorder kinetic model (TCFOKM) and modified two compartment first-order kinetic model (MTCFOKM). Among the models, the MTCFOKM was the best in fitting both sorption and desorption kinetic data, and therefore could predict the most accurately. In MTCFOKM, the fast sorption fraction (f'1, s) increased with the hydrophobicity (Kow) of the PAHs,whereas the fast desorption fraction (f '1, d) decreased. The fast sorption rate constant (k'1, s) was much greater than the slow sorption rate constant (k'2, s). Effect of aging on the desorption kinetics was also analyzed. The f '1, d in MTCFOKM decreased but the slow desorption fraction (f '2, d) increased with aging, indicating that slow desorption is directly related to aging.

Gd effect on microstructure and properties of the Modified-690 alloy for function structure integrated thermal neutron shielding

Zhang Cheng,Pan Jie,Wang Zixie,Wu Zhaoyu,Mei Qiliang,Ding Qianxue,Gao Jing,Xiao Xueshan 한국원자력학회 2023 Nuclear Engineering and Technology Vol.55 No.5

The new Modified-690Gd alloy, namely as Nie30Cr-(10-x) Fe-xGd (x ¼ 0.5, 1.0, 1.5,2.0, 3.0 wt%) for function structure integrated thermal neutron shielding has been prepared and characterized. The Modified-690Gd alloy was mainly composed of g austenite matrix and (Ni, Cr, Fe)5Gd precipitated along grain boundaries. The new Modified-690Gd alloy had great mechanical properties, which had the tensile strength exceeding 620 MPa and the elongation being above 50%. Meanwhile, this alloy had excellent weldability and good corrosion resistance in boric acid. The new Modified-690Gd alloy is expected to be a kind of high efficiency thermal neutron shielding materials

Characterization and performance test of phenolphthalein poly(aryl ether ketone) fractionated by incremental poor solvent fractionation

Zengxu Qian,Jingwei Jiang,Yunlong Sun,Qiliang Yuan,Zhichao Wang,Zhipeng Wang,Guangyuan Zhou 한국고분자학회 2023 Macromolecular Research Vol.31 No.8

Though phenolphthalein poly(aryl ether ketone) (PEK-C) has gained attention as a special engineering plastic materials by virtue of its good thermal stability and favorable mechanical properties, its applications are limited due to its wide PDI during polymerization, which result in poor mechanical properties of PEK-C. In order to investigate the effect of molecular weight and polydispersity index (PDI) on PEK-C, an incremental poor solvent fractionation method was used to fractionate PEK-C. The process produced a series of grades with decreasing molecular weight and PDI. The LCP (linear cloud point) equation was combined with the grading method to find the optimal PEK-C/DMAc/ Non-solvent additives (NSA) system, which can be used to improve the grading efficiency. The obtained results showed that the incremental poor solvent fractionation method with the PEK-C/DMAc/EA system made the molecular weight distributed in the range of 25 ~ 113 kDa, PDI mostly distributed in the range of 1.1 ~ 1.4, and the narrowest PDI to reach 1.13, which could not be obtained through the condensation reaction. Meanwhile, we can also achieve expanded production. By fractionation, the elastic modulus of PEK-C film could be increased by up to 22.6%, elongation at break by up to 38.3%, the fracture strength by up to 38.2%, and impact strength of the sheest by up to 41.2%, while the polymer still maintained good thermal and UV shielding properties. This unique Polymer/Solution/NSA system can yield an effective design strategy for controlling its molecular weight and PDI and improving its mechanical performance.

Intra Mode Selection for Depth Map Coding in 3D-HEVC

Qiuwen Zhang,Ming Chen,Lixun Huang,Qiliang Zhu,Xiaobing Wang,Yong Gan 한국산학기술학회 2014 SmartCR Vol.4 No.5

3D high efficiency video coding was recently standardized by the Joint Collaborative Team on 3D Video coding as an extension of high efficiency video coding. In the current 3D high efficiency video coding method, new depth intra modes including Depth Modeling Modes and Region Boundary Chain coding are used for depth map coding. These partition-based intra modes achieve the highest possible coding efficiency, but take an extremely long time, which hinders the practical application of the new standard. In this paper, we propose an efficient intra mode selection algorithm for depth map coding in 3D high efficiency video coding. It makes use of the coding information from a spatial neighboring depth map treeblock and the co-located texture video treeblock to predict the depth map intra mode treeblock and terminate its mode decision process early. The experimental results show that the proposed intra mode selection algorithm can achieve the average computational savings of about 40% with a negligible loss of rate distortion performance in 3D high efficiency video coding intra-only configuration.