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Madhaiyan, Munusamy,Poonguzhali, Selvaraj,Senthilkumar, Murugaiyan,Pragatheswari, Dhandapani,Lee, Jung-Sook,Lee, Keun-Chul International Union of Microbiological Societies 2015 International journal of systematic and evolutiona Vol.65 No.2
<P>Three novel bacterial strains, designated Vu-144<SUP>T</SUP>, Vu-7 and Vu-35, were isolated on minimal medium from rhizosphere soil of field-grown cowpea and subjected to a taxonomic study using a polyphasic approach. Cells of the strains were Gram-stain-negative, non-motile, non-spore-forming, coccoid rods, and formed non-pigmented colonies. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Vu-144<SUP>T</SUP> was affiliated with an uncultivated lineage of the phylum <I>Bacteroidetes</I>. Its closest phylogenetic neighbour was the recently described species <I>Niastella populi</I>, a member of the family <I>Chitinophagaceae</I>, with just 90.7 % sequence similarity to the type strain. The only isoprenoid quinone detected was menaquinone 7 (MK-7). The fatty acid profiles showed large amounts of iso-C<SUB>15 : 0</SUB>, iso-C<SUB>17 : 0</SUB> 3-OH and iso-C<SUB>15 : 1</SUB> G and minor amounts of summed feature 3 (C<SUB>16 : 1</SUB>ω7<I>c</I> and/or iso-C<SUB>15 : 0</SUB> 2-OH), C<SUB>16 : 0</SUB> and other fatty acids, allowing the differentiation of the strains from other genera. The G+C content of the genomic DNA of the three strains ranged from 43.1 to 44.3 mol%. In addition to phosphatidylethanolamine, the major polar lipids were three unidentified aminophospholipids (APL1–APL3), two unidentified phospholipids (PL1, PL2) and three unidentified lipids (UL1–UL3). Biochemical test patterns also differed from those of <I>Niastella populi</I> and members of other genera. All three isolates showed plant-growth-promoting properties, e.g. the ability to produce indole-3-acetic acid and NH<SUB>3</SUB> and to solubilize phosphate, utilized 1-aminocyclopropane 1-carboxylate (ACC) as a sole source of nitrogen and possessed the ACC deaminase enzyme. The novel isolates readily colonized roots and stimulated growth of tomato and cowpea under glasshouse conditions. Inoculated plants showed a 45–60 % increase in dry matter weight with respect to uninoculated controls. On the basis of the evidence from our polyphasic study, isolate Vu-144<SUP>T</SUP> represents a novel genus and species in the family <I>Chitinophagaceae</I>, for which the name <I>Arachidicoccus rhizosphaerae</I> gen. nov., sp. nov. is proposed. The type strain of <I>Arachidicoccus rhizosphaerae</I> is Vu-144<SUP>T</SUP> ( = KCTC 22378<SUP>T</SUP> = NCIMB 14473<SUP>T</SUP>).</P>
Madhaiyan, M,Poonguzhali, S,Senthilkumar, M,Pragatheswari, D,Lee, K-C,Lee, J-S N.V. Swets en Zeitlinger 2013 Antonie van Leeuwenhoek Vol.103 No.3
<P>A novel plant-associated obligate methylotrophic bacterium, designated strain Ca-68(T), was isolated from the rhizosphere soil of field-grown red pepper from India. The isolates are strictly aerobic, Gram negative, motile rods multiplying by binary fission and formaldehyde is assimilated via the ribulose monophosphate pathway. A comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a clade with the species Methylobacillus flagellatus, Methylobacillus glycogens and Methylobacillus pratensis, with which it showed pairwise similarity of 97.8, 97.4 and 96.2 %, respectively. The major fatty acids are C(16:0), C(10:0) 3OH and C(16:1) ω7c. The G+C content of the genomic DNA is 59.7 mol%. The major ubiquinone is Q-8. Dominant phospholipids are phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Based on 16S rRNA gene sequence analysis and DNA-DNA relatedness (14-19 %) with type strains of the genus Methylobacillus, the novel isolate was classified as a new species of this genus and named Methylobacillus rhizosphaerae Ca-68(T) (=KCTC 22383(T) = NCIMB 14472(T)).</P>