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Application of Ionic Liquids as Mobile Phase Modifier in HPLC
Polyakova Yulia,Koo Yoon-Mo,Row Kyung-Ho The Korean Society for Biotechnology and Bioengine 2006 Biotechnology and Bioprocess Engineering Vol.11 No.1
Ionic liquids are receiving an upsurge of interest as 'green' solvents; primarily as replacements for conventional media in chemical processes. Although ionic liquids are rather 'young' modifier, their great potential in high-performance liquids chromatography (HPLC) has already been demonstrated. This review presents an overview of the applications of ionic liquids as mobile phase modifiers in HPLC.
Barcoding and Phylogenetic Inferences in Nine Mugilid Species (Pisces, Mugiliformes)
Polyakova, Neonila,Boutin, Alisa,Brykov, Vladimir The Korean Society of Systematic Zoology 2013 Animal Systematics, Evolution and Diversity Vol.29 No.4
Accurate identification of fish and fish products, from eggs to adults, is important in many areas. Grey mullets of the family Mugilidae are distributed worldwide and inhabit marine, estuarine, and freshwater environments in all tropical and temperate regions. Various Mugilid species are commercially important species in fishery and aquaculture of many countries. For the present study we have chosen two Mugilid genes with different phylogenetic signals: relatively variable mitochondrial cytochrome oxidase subunit I (COI) and conservative nuclear rhodopsin (RHO). We examined their diversity within and among 9 Mugilid species belonging to 4 genera, many of which have been examined from multiple specimens, with the goal of determining whether DNA barcoding can achieve unambiguous species recognition of Mugilid species. The data obtained showed that information based on COI sequences was diagnostic not only for species-level identification but also for recognition of intraspecific units, e.g., allopatric populations of circumtropical Mugil cephalus, or even native and acclimatized specimens of Chelon haematocheila. All RHO sequences appeared strictly species specific. Based on the data obtained, we conclude that COI, as well as RHO sequencing can be used to unambiguously identify fish species. Topologies of phylogeny based on RHO and COI sequences coincided with each other, while together they had a good phylogenetic signal.
Polyakova, Yulia,Jin, Yinzhe,Zheng, Jinzhu,Row, Kyung Ho Taylor Francis 2006 JOURNAL OF LIQUID CHROMATOGRAPHY AND RELATED TECHN Vol.29 No.12
<P> In this paper, three nucleic acids such as cytosine, cytidine, and thymine and two amino acids, such as D-tryptophan and N-carbobenzyloxy-D-phenylalanine, were chromatographed using ionic liquid as an additive for the mobile phase in high performance liquid chromatography (HPLC). Ionic liquid, 1-butyl-3-methylimidazolium tetrafuloroborate ([BMIm]+ [BF4]-), was used. The nucleic acid eluent was 5 vol. % of the modifier (methanol:acetonitrile=95:5 (vol. %)), in 10 mM of sodium phosphate monobasic, with addition of 0.5, 2.0, and 4.0 mmol/L of ionic liquid. Separation of nucleic acids was obtained on a commercially available octadecyl silica column (4.6×150 mm i.d., and particle size 5 µm). In the case of D-amino acids, the mobile phase was 65% methanol in water, with additions of 0.5, 1.0, 2.0, 8.0, 12.0, and 15.0 mmol/L of ionic liquid. The experiments were performed on a stainless steel column, 3.9×300 mm i.d., and particle size 15 µm, packed with octadecyl-bonded silica at the laboratory. Effects of the concentration of ionic liquid for retention and separation of some nucleic and amino acids were discussed. The results showed the potential application of ionic liquid as a mobile phase additive in liquid chromatography.</P>
QSPR Models for Chromatographic Retention of Some Azoles with Physicochemical Properties
Polyakova, Yulia,Jin, Long Mei,Row, Kyung-Ho Korean Chemical Society 2006 Bulletin of the Korean Chemical Society Vol.27 No.2
This work deals with 24 substances composed of nitrogen-containing heterocycles. The relationships between the chromatographic retention factor (k) and those physicochemical properties which are relevant in quantitative structure-properties relationship (QSPR) studies, such as the polarizability $(\alpha)$, molar refractivity (MR), lipophilicity (logP), dipole moment $(\mu)$, total energy $(E_{tot})$, heat of formation $(\Delta H_f)$, molecular surface area $(S_M)$, and binding energy $(E_b)$, were investigated. The accuracy of the simple linear regressions between the chromatographic retention and the descriptors for all of the compounds was satisfactory (correlation coefficient, $0.8 \leq r \leq 1.0$). The QSPR models of these nitrogen-containing heterocyclic compounds could be predicted with a multiple linear regression equation having the statistical index, r = 1.000. This work demonstrated the successful application of the multiple linear approaches through the development of accurate predictive equations for retention factors in liquid chromatography.
Polyakova, Yulia,Mei Jin, Long,Ho Row, Kyung Taylor Francis 2006 JOURNAL OF LIQUID CHROMATOGRAPHY AND RELATED TECHN Vol.29 No.4
<P>This study evaluates retention factors of 29 nitrogen containing heterocycles using QSPR models in liquid chromatography. Some structure properties, such as the molecular connectivity indices ( 0 ?~ 5 ?), Wiener index ( W ), Kier flexibility index (?), Harary index ( H ), Balaban indices ( JX ~ JY ), and Zagreb indices ( M 1 ~ M 2 ) were obtained by theoretical molecular descriptors derived from information of chemical structures of substances. The relationship between the chromatographic retention factors and the structure descriptors were predicted using a mathematical method, regression analysis. All substances were divided into five groups based on similar structures with functional groups. For each group, the simple linear regression between each structural index and the retention factor showed good regression coefficients. The linear regression between retention factor and various orders of connectivity indices showed good regression coefficient ( r 2 =0.8~1) and, with multiple structural properties, showed r 2 =1. This study has demonstrated the successful linear regression approaches to prediction of the retention factors and some molecular descriptors of the substances.</P>
Barcoding and Phylogenetic Inferences in Nine Mugilid Species (Pisces, Mugiliformes)
Neonila Polyakova,Alisa Boutin,Vladimir Brykov 한국동물분류학회 2013 Animal Systematics, Evolution and Diversity Vol.29 No.4
Accurate identification of fish and fish products, from eggs to adults, is important in many areas. Grey mullets of the family Mugilidae are distributed worldwide and inhabit marine, estuarine, and freshwater environments in all tropical and temperate regions. Various Mugilid species are commercially important species in fishery and aquaculture of many countries. For the present study we have chosen two Mugilid genes with different phylogenetic signals: relatively variable mitochondrial cytochrome oxidase subunit I (COI) and conservative nuclear rhodopsin (RHO). We examined their diversity within and among 9 Mugilid species belonging to 4 genera, many of which have been examined from multiple specimens, with the goal of determining whether DNA barcoding can achieve unambiguous species recognition of Mugilid species. The data obtained showed that information based on COI sequences was diagnostic not only for species-level identification but also for recognition of intraspecific units, e.g., allopatric populations of circumtropical Mugil cephalus, or even native and acclimatized specimens of Chelon haematocheila. All RHO sequences appeared strictly species specific. Based on the data obtained, we conclude that COI, as well as RHO sequencing can be used to unambiguously identify fish species. Topologies of phylogeny based on RHO and COI sequences coincided with each other, while together they had a good phylogenetic signal.
Application of Ionic Liquids as Mobile Phase Modifier in HPLC
Yulia Polyakova,구윤모,노경호 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.1
Ionic liquids are receiving an upsurge of interest as ‘green’ solvents; primarily as replacements for conventional media in chemical processes. Although ionic liquids are rather “young” modifier, their great potential in high-performance liquids chromatography (HPLC) has already been demonstrated. This review presents an overview of the applications of ionic liquids as mobile phase modifiers in HPLC.