Effects of Exogenous Elicitors on Triterpenoids Accumulation and Expression of Farnesyl Diphosphate Synthase Gene in Inonotus obliquus

Pei Lin,Zheng-Fei Yan,Chang-Tian Li 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.4

Exogenous elicitors can influence accumulation of triterpenoids by regulating FPS (farnesyl pyrophosphate synthase) in Inonotus obliquus, such as birch bark (BB), birch rhizosphere soil (BS), Frankia alni (FA), and Rhizobium indigoferae (RI). Among them, the highest yield of biomass (15.6 mg/mL) could be detected by treatment with RI at 2 μg/mL for 10 d, about 2.1-fold of the control. Results showed the significant effect of elicitors on accumulation of triterpenoids by regulating FPS expression level. RI stimulated mycelium to achieve the highest accumulation of triterpenoids about 48.2 mg/g, which was about over 4-fold of the control (9.5 mg/g). Its effect on FPS expression level was greater than that of others, which was about over 10-fold of the control. However, accumulation of triterpenoids and FPS expression level were clearly down-regulated under treatment with BS. In addition, two endogenous factors (H2O2 and NO) in I. obliquus could affect accumulation of triterpenoids by regulating FPS expression level. Effect of RI on H2O2 and NO contents were higher than that of others, about 3.5-fold of the control. The minimum value of H2O2 content was detected by treatment with BS for 10 d, which was about 0.5-fold of the control. RI that assist with CAT (catalase) and SNP (sodium nitroprusside dehydrate) could stimulate triterpenoid contents to achieve highest accumulation, which was about 5.5-fold of the control. Fluctuation of H2O2 and NO content seems to play a pivotal role in accumulation of triterpenoids by regulating FPS expression level.

Dynamic Heart

Pei Lin,Feng Shu 한국디자인학회 2023 한국디자인학회 국제초대전도록 Vol.2023 No.10

An Enhancement of Antibacterial Activity and Synergistic Effect of Biosynthesized Silver Nanoparticles by Eurotium cristatum with Various Antibiotics

Pei Lin,Fu-Qing Wang,Chang-Tian Li,Zheng-Fei Yan 한국생물공학회 2020 Biotechnology and Bioprocess Engineering Vol.25 No.3

In this study, biosynthesis of silver nanoparticles (AgNPs) by using Eurotium cristatum, isolated from Fuzhuan brick-tea and its antibacterial activity has been demonstrated. AgNPs were characterized at 425 nm as maximum absorbance peak by ultraviolet-visible spectrophotometry. The images of transmission electron microscopy revealed that AgNPs are spherical shape with at 15-20 nm in size. The X-ray diffraction pattern corresponding to planes (111), (200), (220), (311), and (222) demonstrated the crystalline nature of AgNPs. Fourier transform infrared spectrum showed that functional groups involved in reduction of silver ions to metal nanoparticles. For antibacterial application, AgNPs showed antibacterial activity against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Candida albicans, and Bacillus subtilis. It also acted synergistically with various antibiotics to inhibit growth of pathogenic strains, which produced an effect greater than the sum of their individual effects. For neomycin with no resistance to C. albicans, it combined with AgNPs, which had significant synergistic effect against C. albicans, with maximum inhibitory zone at 20.9 mm, which was 2.5-fold greater than that of AgNPs alone (8.2 mm). Other antibiotics combined with AgNPs also existed similar synergistic effect. Therefore, AgNPssynthesized by E. cristatum could enhance antibacterial activity in combination with antibiotics against pathogenic strains through synergistic effects. It might provide a new strategy for treatment of resistant bacteria.

Existence-and-Uniqueness and Mean-square Boundedness of the Solution to Stochastic Control Systems

Peilin Lu,Caixia Gao 한국전산응용수학회 2013 Journal of applied mathematics & informatics Vol.31 No.3

This paper mainly deals with the stochastic control system, the existence and uniqueness of solutions and the behavior of solutions are investigated. Firstly, we obtain sufficient conditions which guarantee the existence and uniqueness of solutions to the stochastic control system. And then, boundedness of the solution to the system is achieved under mean-square linear growth condition.

EXISTENCE-AND-UNIQUENESS AND MEAN-SQUARE BOUNDEDNESS OF THE SOLUTION TO STOCHASTIC CONTROL SYSTEMS

Lu, Peilin,Cao, Caixia The Korean Society for Computational and Applied M 2013 Journal of applied mathematics & informatics Vol.31 No.3

This paper mainly deals with the stochastic control system, the existence and uniqueness of solutions and the behavior of solutions are investigated. Firstly, we obtain sufficient conditions which guarantee the existence and uniqueness of solutions to the stochastic control system. And then, boundedness of the solution to the system is achieved under mean-square linear growth condition.

Great Transformation: End of the Villages : A Study of the Villages Located in Southern Urban China

Li Peilin 한국사회학회 2003 한국사회학회 기타간행물 Vol.- No.-

CONSUMPTION STRATIFICATION IN CHINA: AN IMPORTANT TOOL IN STIRRING UP ECONOMY

LI PEILIN,ZHANG YI 서울대학교 사회발전연구소 2000 Journal of Asian Sociology Vol.29 No.2

The Clinical Characteristic and Management of Patients with Nocardiosis in a Tertiary Hospital in China

Liu Peilin,Wang Zhiqian,Jian Zijuan,Liu Xuan,Li Yanming,Yan Qun,Zhong Baiyun,Liao Mengting,Liang Xianghui,Liu Wenen 한국미생물·생명공학회 2023 Journal of microbiology and biotechnology Vol.33 No.5

Nocardiosis is an uncommon opportunistic bacterial infection which becomes a significant health problem due to its increasing incidence and high mortality rate. However, many nocardiosis patients are underdiagnosed by physicians. To summarize the clinical characteristics and management of nocardiosis would help with better diagnosis and prognosis of nocardiosis. This retrospective study was conducted based on the medical records of nocardiosis patients between January 2015 and December 2021 in a tertiary hospital in China. Overall, 44 nocardiosis patients with 54 specimens were included. The patients consisted of 26 males and 18 females with a mean age of 50.4 ± 13.2 years. Among 44 patients, 26 (59.1%) were previously given immunosuppressive therapy. Connective tissue diseases (CTDs) were the most common underlying disease (16/44). The most frequent infection sites were the lungs (17/44) and skin or soft tissues (8/44). Common symptoms included cough (23/44), expectoration (18/44), fever (15/44), and subcutaneous abscesses (15/44). Forty-five out of 54 specimens (83.3%) required over 48 hours of culture time for nocardiosis detection. Thirty-six patients were cured or improved, 5 patients were discharged from the hospital due to poor prognosis, and 1 patient died. The average diagnosis time of poor prognosis cases was 19.7 days, which was significantly longer than those of improved or cured patients (7.3 days). Immunosuppressed patients comprise a large part of nocardiosis cases, which is worth attention in clinical practice. Early diagnosis, specifically through prolonged cultivation time of specimen, could help achieve better prognosis of nocardiosis patients.

中国傳統《楚辞》硏究小考

?沛霖(Zhao Peilin),李宇正(Lee Woo-jeong) 중국어문학연구회 2007 중국어문학논집 Vol.0 No.47

본 연구는 기존의 楚辭硏究의 성과와 면모를 돌아보고 총괄함으로서 초사연구의 새로운 지향을 모색하고자 한다. 그 중요한 성과와 특징을 개괄해 보면, 초사연구사는 대략 漢에서 唐, 宋, 그리고 元에서 淸의 세 단계로 정리가 가능하다. 漢에서 唐에 이르는 기간에 초사는 책으로 정리되었으며, 屈原과 그의 작품에 관한 기초적인 자료가 소개되었다. 이를 토대로 한 초보적인 해석이 이루어지고, 굴원의 高尙한 人格이 높이 평가되었다. 魏晉에 이르러 문학에 대한 관념이 발전함에 따라 초사를 바라보는 시각이 ‘經學’에서 ‘文學’으로 변화되기는 하였지만, 대체로 이 시기는 楚辭學이 성립되는 시기로 생각된다. 宋代에는 ‘疑古思辨’의 기풍이 성행하였으며, 초사 연구도 그 영향을 받아 새로운 성과를 도출하였다. 전대의 연구 성과를 계승하기는 하였지만, 굴원의 고상한 인격 가운데 ‘忠君愛國’을 강조함으로서 봉건윤리의 쳬계 속에 편입시켜 그 한계를 드러내기도 하였다. 그러나, 초사를 남방문화의 산물로 파악하여 초사연구를 심화시킨 것이나, ‘疑古思辨’의 기풍 아래 기존의 견해를 반박하고 새로운 견해를 제기하는 과감한 治學態度는 주목할 만하다. 元에서 淸에 이르는 시기에는 초사에 관한 연구서도 많이 출간되었고, 새로운 관점도 많이 제기되었을 뿐 아니라, 이들 사이에 격렬한 논쟁이 전개되었다. 우선 “朕?國家”라는 관념이 부정됨에 따라, 굴원사상의 중점이 봉건윤리화한 ‘忠君愛國’에서 ‘憂國憂民’으로 바뀌었다. 아울러 초사의 예술적인 形象이나 構造를 주목하는 한편, 文字、訓?、審音、考證 등의 과학적인 연구방법을 적용하였다. 그리고 굴원의 생애와 작품의 배경에 관한 연구도 치밀하게 전개하여 역사와 사회의 관점에서 초사에 대한 이해의 심도를 제고하였다. 淸末에 서양의 문학관이 중국에 들어옴에 따라 문학에 대한 관념이 크게 변화하였고, 초사를 바라보는 시각도 변화함에 따라 각종 초사연구가 이루어지고, 전통 초사연구의 시각을 벗어나는 다양한 관점이 제기되었다. 그러나 총체적으로 볼 때. 이들 연구조차도 기존의 연구 성과를 종합적으로 정리한 것이 대부분이며, 학계 전체의 관심과 동의를 이끌어 낼만한 연구는 드물다고 본다. 즉, 초사연구는 아직도 봉건적인 시각에서 그다지 벗어나 있지 않으며, 그 속박으로부터 자유롭지 못하다. 연구 방법에 있어서도 오랫동안 축적되어온 牽强附會나 主觀臆斷의 비합리성이라는 그늘에서 완전히 벗어난 것은 아니라고 생각한다.

Influence of Lateral Pressure on the Mechanical Performance of Bolted Specimens under Biaxial Compression

Shuxue Ding,Peilin Jiang,Jing Qiao,Hua Nan,Lei Xu,Jiaqi Guo,Sijiang Wei 대한토목학회 2024 KSCE Journal of Civil Engineering Vol.28 No.1

The influence of lateral pressure (2) on the stability of the surrounding rock of roadway increases after excavation. The rock bolt is usually used to improve the stability of the surrounding rock. Therefore, it is important to understand the biaxial compressive behaviors of the bolted samples influenced by lateral pressure. In this study, biaxial compression experiments are carried out to investigate the biaxial compressive properties of bolted rock samples influenced by lateral pressure. The results show that bolting can improve the peak strength, inhibit the post-peak strength attenuation and reduce the pre-peak brittleness of the bolted rock mass. With the increase of lateral pressure, the strength and elastic modulus of the bolted samples increases approximately parabolic and linearly, respectively. The failure modes of the bolted samples influenced by lateral pressure can be divided into splitting failure and compression-shear failure. A reinforcement mechanism of bolt is observed, namely axial action, axial and transverse action at the pre- and post-peak stages of the bolted samples. Based on a modified true triaxial Mohr-Coulomb strength criterion and this bolt reinforcement mechanism, a new method for calculating the equivalent cohesion and equivalent internal friction angle of the bolted specimens under biaxial compression is proposed. The research results of this paper have certain reference value for the stability control of roadway