Donor-Acceptor (D-A)-based Conductive Polymers for Energy Storage ApplicationsAuthors: Rajkumari Linthoinganbi, Soojin Park, Dhananjaya Patra, Sungjune Park.

( Rajkumari Linthoinganbi ),( Soojin Park ),( Dhananjaya Patra ),( Sungjune Park ) 한국공업화학회 2022 한국공업화학회 연구논문 초록집 Vol.2022 No.-

Seasonal Variability of Marine Fish Diversity in Relation to Water Quality of East Midnapore Coast of West Bengal, India

Chini Deep Sankar,Mondal Niladri,Kar Avijit,Bunholi Ingrid,Singh Sourav,Ghosh Pratik,Patra Prasanta,Patra Shampa,Patra Bidhan Chandra 한국해양과학기술원 2023 Ocean science journal Vol.58 No.2

Marine fishes are one of the important factors in stabilizing the local aquatic ecosystem and regulating the nutritional socioeconomy of local fisher folks. The recent increases in anthropogenic activity, pollution and overfishing have led to the decline of marine fish species richness and their local aquatic habitats. In this study we sought to determine the inter-relationship between water quality, anthropogenic activity, and fish landing stations through a 31 km stretch of the East Midnapore coast in West Bengal, India which is known for its tourist destinations. The study was conducted monthly on different trawler fish landing sites from Dec 2018 to Dec 2021. During this period, we took fish samples and identified them. We obtained water quality data regarding Sea Surface Temperature (SST), Concentration of Chlorophyll-a (Chl-a), Turbidity, and Dissolved Oxygen (DO) in order to identify further correlation between the water quality analysis and species diversity. 154 numbers of commercially important marine fish species were documented. As per the IUCN database, 13% of the total fish species fall under the red list category and 16% of the species reveal a decreasing population trend. The availability of those red-listed fish throughout the season has been shown in the matrix plot to detect their gradual decrease in sighting. After analyzing the water quality data, we found out that DO, SST, Turbidity, and Chl-a correlate with the species richness on some sites and the water parameters are also differs during the seasons. Both fish species richness and water quality have been affected on those fish landing sites which have been subject to heavy anthropogenic loads.

Phylogenetic Relationship between Symbionts of Tubeworm Lamellibrachia satsuma and the Sediment Microbial Community in Kagoshima Bay

Patra, Ajit Kumar,Cho, Hyun Hee,Kwon, Yong Min,Kwon, Kae Kyoung,Sato, Takako,Kato, Chiaki,Kang, Sung Gyun,Kim, Sang-Jin Korean Ocean Research & Development Institute and 2016 OCEAN SCIENCE JOURNAL Vol.51 No.3

Vestimentiferan tubeworms acquire their symbionts through horizontal transmission from the surrounding environment. In the present study, we constructed a 16S rRNA gene clone library to investigate the phylogenetic relationship between diverse microbes in the sediment and symbiotic bacteria in the trophosome of the tubeworm, Lamellibrachia satsuma, from Kagoshima Bay, Japan. Two symbiotic bacterial phylotypes belonging to the classes <TEX>${\gamma}$</TEX>- and <TEX>${\varepsilon}$</TEX>-Proteobacteria were found from this tubeworm trophosome. They were very closely related to the symbionts of several other marine invertebrates. The most predominant bacteria in the sediment were <TEX>${\varepsilon}$</TEX>-Proteobacteria. A broad diversity of bacteria belonged to non-proteobacterial phyla such as Planctomycetes, Acidobacteria, and Chloroflexi was observed. The presence of sulfur oxidizers (i.e., <TEX>${\varepsilon}$</TEX>-Proteobacteria and <TEX>${\gamma}$</TEX>-Proteobacteria) and sulfur reducers (i.e., <TEX>${\delta}$</TEX>-Proteobacteria) may play a significant role in the sulfur cycle in these habitats and provide multiple sources of nutrition to the cold-seep communities. Closely related clones of <TEX>${\varepsilon}$</TEX>-Proteobacteria symbiont in the species level and of <TEX>${\gamma}$</TEX>-Proteobacteria symbiont in the genus level were found in the surrounding sediment. The similarity of symbiont clones of L. satsuma with other symbionts and free-living bacteria suggests the possibility of opportunistic symbiosis in <TEX>${\varepsilon}$</TEX>-Proteobacteria and the co-evolution of <TEX>${\gamma}$</TEX>-Proteobacteria having occurred after symbiosis with the tubeworms.

Complete gammaproteobacterial endosymbiont genome assembly from a seep tubeworm Lamellibrachia satsuma

Patra Ajit Kumar,Kwon Yong Min,Yang Youngik 한국미생물학회 2022 The journal of microbiology Vol.60 No.9

Siboglinid tubeworms thrive in hydrothermal vent and seep habitats via a symbiotic relationship with chemosynthetic bacteria. Difficulties in culturing tubeworms and their symbionts in a laboratory setting have hindered the study of host-microbe interactions. Therefore, released symbiont genomes are fragmented, thereby limiting the data available on the genome that affect subsequent analyses. Here, we present a complete genome of gammaproteobacterial endosymbiont from the tubeworm Lamellibrachia satsuma collected from a seep in Kagoshima Bay, assembled using a hybrid approach that combines sequences generated from the Illumina and Oxford Nanopore platforms. The genome consists of a single circular chromosome with an assembly size of 4,323,754 bp and a GC content of 53.9% with 3,624 protein-coding genes. The genome is of high quality and contains no assembly gaps, while the completeness and contamination are 99.33% and 2.73%, respectively. Comparative genome analysis revealed a total of 1,724 gene clusters shared in the vent and seep tubeworm symbionts, while 294 genes were found exclusively in L. satsuma symbionts such as transposons, genes for defense mechanisms, and inorganic ion transportations. The addition of this complete endosymbiont genome assembly would be valuable for comparative studies particularly with tubeworm symbiont genomes as well as with other chemosynthetic microbial communities.

Green Nanobiotechnology: Factors Affecting Synthesis and Characterization Techniques

Patra, Jayanta Kumar,Baek, Kwang-Hyun Hindawi Limited 2014 Journal of nanomaterials Vol.2014 No.-

<P>Nanobiotechnology is gaining tremendous impetus in this era owing to its ability to modulate metals into their nanosize, which efficiently changes their chemical, physical, and optical properties. Accordingly, considerable attention is being given to the development of novel strategies for the synthesis of different kinds of nanoparticles of specific composition and size using biological sources. However, most of the currently available techniques are expensive, environmentally harmful, and inefficient with respect to materials and energy use. Several factors such as the method used for synthesis, pH, temperature, pressure, time, particle size, pore size, environment, and proximity greatly influence the quality and quantity of the synthesized nanoparticles and their characterization and applications. Additionally, characterization of the synthesized nanoparticles is essential to their potential use in various drug delivery and biomedical applications. The present review highlights various parameters affecting the synthesis of nanoparticles by green nanobiotechnology and different techniques used for characterizing the nanoparticles for their potential use in biomedical and environmental applications.</P>

A novel histone deacetylase (HDAC) inhibitor MHY219 induces apoptosis via up-regulation of androgen receptor expression in human prostate cancer cells

Patra, N.,De, U.,Kim, T.H.,Lee, Y.J.,Ahn, M.Y.,Kim, N.D.,Yoon, J.H.,Choi, W.S.,Moon, H.R.,Lee, B.M.,Kim, H.S. Masson Pub. USA, Inc 2013 Biomedicine & pharmacotherapy Vol.67 No.5

Histone deacetylase (HDAC) inhibitors are a new class of anticancer agents that act by inhibiting cancer cell proliferation and inducing apoptosis in various cancer cell lines. To investigate the anticancer effect of a novel histone deacetylase (HDAC) inhibitor MHY219, its efficacy was compared to that of suberoylanilide hydroxamic acid (SAHA) in human prostate cancer cells. The anticancer effects of MHY219 on cell viability, HDAC enzyme activity, cell cycle regulation, apoptosis and other biological assays were performed. MHY219 was shown to enhance the cytotoxicity on DU145 cells (IC<SUB>50</SUB>, 0.36μM) when compared with LNCaP (IC<SUB>50</SUB>, 0.97μM) and PC3 cells (IC<SUB>50</SUB>, 5.12μM). MHY219 showed a potent inhibition of total HDAC activity when compared with SAHA. MHY219 increased histone H3 hyperacetylation and reduced the expression of class I HDACs (1, 2 and 3) in prostate cancer cells. MHY219 effectively increased the sub-G1 fraction of cells through p21 and p27 dependent pathways in DU145 cells. MHY219 significantly induced a G2/M phase arrest in DU145 and PC3 cells and arrested the cell cycle at G0/G1 phase in LNCaP cells. Furthermore, MHY219 effectively increased apoptosis in DU145 and LNCaP cells, but not PC3 cells, according to Annexin V/PI staining and Western blot analysis. These results indicate that MHY219 is a potent HDAC inhibitor that targets regulating multiple aspects of cancer cell death and might have preclinical value in human prostate cancer chemotherapy, warranting further investigation.

A novel epoxypropoxy flavonoid derivative and topoisomerase II inhibitor, MHY336, induces apoptosis in prostate cancer cells

Patra, Nabanita,De, Umasankar,Kang, Jin-Ah,Kim, Ji Mim,Ahn, Mee Young,Lee, Jaewon,Jung, Jee H.,Chung, Hae Young,Moon, Hyung Ryong,Kim, Hyung Sik Elsevier 2011 european journal of pharmacology Vol.658 No.2

<P><B>Abstract</B></P><P>Here, we reported the synthesis of a novel topoisomerase II inhibitor, MHY336, which that has strong topoisomerase-mediated anticancer activity but fewer side effects than other topoisomerase II inhibitors. The catalytic activity of MHY336 on the topoisomerase II enzyme was the same as that of the etoposide. In a cell-free system, MHY336 exhibited a potent activity on scavenging of reactive oxygen species against 3-morpholinosydnonimine hydrochloride (SIN-1)-induced oxidative stress. An <I>in vitro</I> cell-based assay demonstrated that MHY336 significantly inhibited the proliferation of three prostate cancer cell lines, LNCaP, PC-3, and DU145 cells. Notably, the cytotoxicity of MHY336 was more potent in LNCaP cells (IC<SUB>50</SUB>=1.39μM) than in DU145 (IC<SUB>50</SUB>=2.94μM) and PC3 cells (IC<SUB>50</SUB>=3.72μM). Furthermore, MHY336 treatment induced similar levels of cytotoxicity compared to doxorubicin treatment (IC<SUB>50</SUB>=1.55μM) in LNCap cells. Also, MHY336 significantly down-regulated topoisomerase II alpha expression and up-regulated p53 expression in LNCaP cells (wild-type p53), whereas it up-regulated the topoisomerase II alpha protein in both DU145 and PC3 cells (p53 mutated or deleted). MHY336 induced G2/M or S phase arrest in LNCaP cells through a well-documented topoisomerase II-dependent mechanism. Further studies using Annexin V-FITC binding assay, DAPI staining, and Western blot analyses illustrated that MHY336 markedly induced apoptotic cell death via the mitochondria-mediated intrinsic pathway in LNCaP cells. These results suggest that MHY336 is an attractive chemotherapeutic agent because of its topoisomerase II-mediated anti-tumour activity in human prostate cancer.</P>

RICCI SOLITONS AND RICCI ALMOST SOLITONS ON PARA-KENMOTSU MANIFOLD

Patra, Dhriti Sundar Korean Mathematical Society 2019 대한수학회보 Vol.56 No.5

The purpose of this article is to study the Ricci solitons and Ricci almost solitons on para-Kenmotsu manifold. First, we prove that if a para-Kenmotsu metric represents a Ricci soliton with the soliton vector field V is contact, then it is Einstein and the soliton is shrinking. Next, we prove that if a ${\eta}$-Einstein para-Kenmotsu metric represents a Ricci soliton, then it is Einstein with constant scalar curvature and the soliton is shrinking. Further, we prove that if a para-Kenmotsu metric represents a gradient Ricci almost soliton, then it is ${\eta}$-Einstein. This result is also hold for Ricci almost soliton if the potential vector field V is pointwise collinear with the Reeb vector field ${\xi}$.

Influence of granulated blast furnace slag as fine aggregate on properties of cement mortar

Patra, Rakesh Kumar,Mukharjee, Bibhuti Bhusan Techno-Press 2018 Advances in concrete construction Vol.6 No.6

The objective of present study is to investigate the effect of granulated blast furnace slag (GBS) as partial substitution of natural sand on behaviour of cement mortar. For this, the methods of factorial design with water cement (w/c) ratio and incorporation percentages of GBS as replacement of natural fine aggregate i.e., GBS(%) as factors are followed. The levels of factor w/c ratio are fixed at 0.4, 0.45, and 0.5 and the levels of factor GBS(%) are kept fixed as 0%, 20%, 40%, 60%, 80% and 100%. The compressive strength (CS) of mortar after 3, 7, 14, 28, 56 and 90 days, and water absorption (WA) are chosen as responses of the study. Analysis of variance (ANOVA) of experimental results has been carried out and those are illustrated by ANOVA tables, main effect and interaction plots. The results of study depict that the selected factors have substantial influence on the strength and WA of mortar. However, the interaction of factors has no substantial impact on CS and WA of mixes.

Immunocompetence Status of White Plumage Naked Neck versus Normally Feathered Broilers in Tropical Climate

Patra, B.N.,Bais, R.K.S.,Sharma, D.,Singh, B.P.,Prasad, R.B.,Bhushan, B. Asian Australasian Association of Animal Productio 2004 Animal Bioscience Vol.17 No.4

The study was undertaken to evaluate the effect of naked neck gene on mortality, cell mediated and humoral immune response in white plumage broiler population. The mortality of homozygous naked neck (Na/Na) broilers (11.71%) was comparatively lower than that of heterozygous naked neck (Na/na) (12.28%) and normally feathered (na/na) (13.59%) broilers. The humoral immune response was measured against (1% v/v) sheep red blood cells (SRBC) for total haemagglutinin (HA) antibody, 2-mercaptoethanol resistance (MER) or (IgG) antibody and 2-mercaptoethanol sensitive (MES) or (IgM) antibody titre on 7 days post-immunization. The titre was expressed as log2 of the highest dilution which shows complete haemagglutination. Total HA titers of Na/Na and Na/na (11.05$\pm$0.53 and 11.09$\pm$0.38) were comparatively higher than that of na/na (10.26$\pm$0.42). The MES antibody titre of Na/Na (8.50$\pm$0.53) and Na/na (7.63$\pm$0.45) broilers were significantly higher as compared to na/na (6.11$\pm$0.32) broilers. The MER titre of na/na genetic group (4.15$\pm$0.42) was significantly higher than Na/Na (2.55$\pm$0.37) and comparatively higher than Na/na (3.45$\pm$0.38) broilers. In vivo cell response to phytohaemagglutinin-P (PHA-P), measured as Foot Index (FI) in mm expressed significantly higher response in Na/na (0.473$\pm$0.05) and Na/Na (0.413$\pm$0.04) broilers as compared to na/na (0.304$\pm$0.03) broilers. The result of present study suggested that white plumage naked neck broilers had better immune response as compared to normally feathered broilers.