Mortierella nana의 균체지질중의 γ-Linolenic acid 함량에 미치는 비타민의 영향

박준권,임무현 대구대학교 산업기술연구소 2003 産業技術硏究 Vol.14 No.3

Mortiella nana속의 미생물을 이용한 균체지질중의 GLA생산에서 배양배지내 butyric acid, 수용성 및 지용성 비타민류를 첨가하여 이들 미량물질의 인자가 균의 생육, 지질생산 및 균체지질중의 지방산 변화와 GLA 함량변화에 미치는 영향을 검토하였다. Mortiella nana속의 배양에 각종 수용성비타민을 첨가한 결과, thiamin, riboflavin, niacinamide, pyridoxin의 첨가구에서 균체지질과 GLA함량이 높았다. 수용성 비타민중 thiamin의 농도를 달리하여 첨가한 결과 0.5㎍/㎖의 첨가구에서 지질함량 및 GLA 함량이 높게 나타났다. Ribofalvin, niacinamide, pyridoxin을 각각 농도별로 첨가한 경우 균체지질 함량 면에서 대조구보다 높았으나 GLA함량은 대조구보다 낮게 나타나, 균체지질의 생산에는 유리하게 작용하였지만 GLA의 생산에는 영향을 미치지 못하였다. n-alkane계의 butyric acid를 농도별로 첨가후 배양한 결과 05㎍/㎖의 농도의 첨가구에서 지질생산량은 0.43g이었고 GLA생산량에서는 18.5%로 무첨가구보다 2배이상의 생산량을 보여 균체지질 및 GLA생산에 큰 영향을 주는 것으로 나타났다. 지용성 비타민 중 tocopherol과 squalene을 배지에 첨가후 배양한 결과, 모든 농도의 첨가구에서 지질 생산량 및 GLA생산이 무첨가구보다 낮게 나타났다. 배양배지의 초기 pH의 영향을 조사한 결과 pH 6.0의 배양 처리구가 지질함량 0.45g, GLA생산량이 17.95%로 가장 최적의 초기배양 pH였으며 배지의 양과 배양속도를 달리하여 산소공급의 영향을 조사한 결과, 100rpm에서 150㎖의 배지로 배양한 처리구가 균체지질 및 GLA생산량이 높았으며, 배양 6일째 처리구가 균체 지질 및 GLA 생산성이 가장 높게 나타났다. The effects of water soluble and fat soluble vitamins on the content of γ-linolenic acid(GLA) in the mycelial lipid by culture of Mortiella nana, were investigated. Addition of water soluble vitamins, thiamin, riboflavin niacinamide and pyridoxin, in the culture medium of Mortiella nana resulted in the increase of mycelial lipid and GLA content, In the addition of 0.5㎍/㎖ thiamin to the basal medium, mycelial lipid and GLA content were the highest. In the each addition concentration of riboflavin niacimide and pyridoxin, mycelial content were more higher than control, but GLA content were more lower. It was addition of riboflavin niacinamide and pyridoxin found that mycelial lipid production was favorable, but GLA production was not affected. In the addition of 0.5㎍/㎖ butyric acid as the n-alkane to the basal medium containing 0.5㎍/㎖ thiamin, mycelial content was 0.43g/g dry cell weight and It was found 18.5% of GLA production that addition of 0.5㎍/㎖ was more twice higher than blank. It was appeared that addition of butyric acid was affected to mycelial lipid and GLA production. In the addition of fat soluble vitamin, tocopherol and squalene, mycelial lipid and GLA production were lower than blank at all added concentration. At the influence of intial pH of culture medium, pH 6.0 of culture medium was optimum, the following results were 0.45g of mycelium lipid and 17.95% of GLA production. The influence of oxygen applying with the various of medium volume and two shaking speed were also examed, When cultivation was cultured for 6 days with 150㎖ volume of medium at 100rpm, mycelium lipid and GLA production were the highest.

재래(흑색) 염소의 비육에 관한 연구 : Ⅰ. 일측 갑상선 제거가 체중, 체온 및 맥박에 미치는 영향 Ⅰ. Effect of Uni-lateral Thyroidectomy on Body Weight, Body Temperature and Pulse Rate

박무현,권해병,양일석 慶尙大學校 1975 論文集 Vol.14 No.-

In order to investigate the relationship of body weight, body temperature and pulse rate to hypothyroidism in the state of uni-lateral thyroidectomy of Korean native goat, a brief observation was carried out. Twenty healthy male adult goats (7 months old) were used in the present experiment dividing into 2 groups, the control with 5 and the experimental with 15, respectively. Individuals of the experimental group were received uni-lateral thyroidectomy by surgical operation, while individuals of the control group, on the other hand, were received sham-operation. Measurements of body weight, body temperature and pulse rate after the operation were performed in duration of 48 days by 2 days interval. The results obtained could be summerized as follows. 1. There were no significant changes in body weight of experimental group compared to that of control group. 2. Uni-laterally thyroidectomized goat showed no changes in body temperature, which is seemed to he doe to a compensatory effect. 3. Statistical calculation did not show any difference in pulse rate between control and experimental group. The present experiment can he concluded that uni-lateral thyroidetomy had not any effect on the changes body weight, body temperature and pulse rate.

절수에 의한 Mongolian Gerbil 장기중량변화에 관한 분석연구

김무강,이기훈,이강이,송치원,이경열,권효정,박미선,정승혁,이행연,김명철 충남대학교 수의과대학 동물의과학연구소 2000 動物醫科學硏究誌 Vol.8 No.-

In this study, authors measured the each organ weight of the long term water deprived Mongolian gerbil, after then we calculated the mean, standard deviation, variance coefficient of the real measured organ weight and induced the organ weight change quantity, rate of quantity, deviation between measured and theological organ weight, deviation rate, sum of deviation rate, organ weight change rate, rate of organ weight by the mathematical formula. The results obtained as follow 1. The weight of the brains, lung, and testis were abruptly decreased after water deprived until the 5th day, after then slowly decreased until the 20th day. 2. The weight of the heart was decreased gradually from the first day until the 20th day. 3. The weight of the liver was abruptly decreased until the 10th day, after then the more slowly decreased until the 20th day. 4. The weight of the thymus, spleen, kidney, and pancreas were abruptly decreased until the 15th day, after then increased slowly until 20th day but the weight of the kidney was abruptly increased from the 10th day. 5. The organ weight change rate and the rate of the each day organ weight were very diversified.

K 대학병원 서어비스에 대한 소비자 만족도

이태섭,박종원,이무식,윤능기,이충원,서석권 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.3

Authors administered a questionnaire to 300 clients visited to the K University Hospital during August in 1992 to investigate the degree and related factors of consumer's satisfaction with the hospital services. Clients consisted of 82 in-patients and 158 out-patients, 52.4 percent of them was male. Four dimensions of satisfaction were assessed which were medical management, administration, environment and attitude. In-patients reported as dissatisfied with the waiting time during receipt, investigative medical procedures and pharmacy (64.7%), lack of conveniency facilities for patients and their guardians(48.8%) and provision of conveniency facilities in the ward(42.4%), unsanitary state of out- and in-hospital(41.5%). Out-patients reported dissatisfaction with the course of medical management and the time needed(70.3%), the waiting time during receipt, investigative medical procedures and pharmacy(68.3%), lack of conveniency facilities for patients and guardians(42.4%). But both in-patients and out-patients were satisfied with medical equipments, doctor's diagnosis reliability and medical effects, technician's attitude more than 50%. Canonical correlation analysis was done to find related sociodemographic factors to the four dimensions of satisfaction. Of the four canonical functions, the first was only statistically significant in in-patients. Its eigen value was 0.56 and its canonical correlation coefficient was 0.60. Satisfaction with environment and medical management was selected as important concept in dependent variables. Standardized canonical function of environment and medical management were 0.76 and 0.35 respectively. It was related with woman with high educational attainment and increasing number of hospital visits. In out-patients, the first, second and third function were statistically significant and their eigen values were 0.18, 0.4, 0.08 respectively which were small compared to those of in-patients. Their canonical correlation coefficient were 0.40, 0.35, 0.28 respectively. Low degree of environment but high degree of medical management satisfaction was noted in the first function which was related with old age, woman and professional occupation. Low degree of satisfaction with administration(second function) was related with city resident, non-professional occupation and young age. High degree of medical management but low degree of administration satisfaction(third function) was related with surgical visitor, man and non-professional occupation. The direction of hospital managerial strategies could be planned and general characteristics of dissatisfactory clients might be suggested with these results.

복합열화환경 하에서의 단면복구재의 철근부식특성 평가에 관한 실험적 연구

김영덕,박종호,조봉석,김용로,권영진,김무한 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

The duties of the restorative material are to bear up against stress and to protect reinforcement corrosion. So the restorative material are estimated by various kinds of strength, permeability and etc. But to evaluate performance of the restorative material accurately, it is necessary to estimate their reinforcement corrosion directly in environment of combined deterioration. In this study, to evaluate performance of restorative materials, we compare their properties in terms of compressive and bending strength and permeability of water and air with actual reinforcement corrosion like ratio of corrosion area, weight reduction and corrosion velocity of steel bar under environment of complex deterioration. The results showed that strength and permeability of restorative materials are similar but their reinforcement corrosions are very different. And there is a case that strength and permeability of restorative material are better than normal concrete but reinforcement corrosions are worse.

국제수도협회(IWA) Specialist Conference 참관기 "Nano and Micro Particles in Water and Wastewater Treatment"

이상협,권순범,박규홍,한무영 대한상하수도학회 2003 상하수도학회지 Vol.17 No.5

Fosfomycin의 항균효과 및 capillary electrophoresis에 의한 측정법에 관한 연구

윤효인,박승춘,이규승,권진욱,조준형,신광순,김무열,허강준,조명행 충남대학교 수의과대학 동물의과학연구소 1996 動物醫科學硏究誌 Vol.4 No.-

Fosfomycin, a low molecular antibiotic, is to be easily synthesized, therefore to be possibly used in veterinary sectors due to econimic reasons. In this study, we determined the antibacterial activities of fosfomycin against some domestic animal-origin pathogens, and its combined effects with some important antibacterials available. We also compared detection methods of fosfomycin from various measuring instruments. The results obtained through this experiment were as follows: 1. Antibacterial activities of fosfomycin against the pathogens used in this experiment were appeared fairly low. We found the antobacterial activities depanded on the kinds of media and the addition of some material thereins, which suggests the importance of appropriate medium so as to judge the antibacterial activity of fosfomycin. 2. Fosfomycin showed varying antibacterial effects in combination with other antibacterials, which should be taken into consideration when combining other antobacterials especially in the clinical settings. Of the combined antivacterials, fosfomycin had synergistic effects in combination with amoxicillin but antagonistic effects with oxytetracyline. 3. Conventional detection methods using HPLC, GC and spectrophotometer were not appropriate to measure fosfomycin. Capillary electrophoresis by the way of the indirect ultraviolet detection method was good enough to detect fosfomycin in the range of expected concentration in tissues, with the limit of quantitation (LOQ) of 1.25 ㎍/㎖. We recommend the eletrophoresis method could be used to detect fosfomycin, thereby being able to set the optimal dosage for animals and providing the measures in relation with the residual concentration in the meat.

동해지방의 천연 Zeolite 의 건조제로서의 흡착특성

김종택,이무섭,박순권,홍상표 경북대학교 산업기술연구소 1988 産業技術硏究誌 Vol.16 No.-

Some selected natural zeolites obtained from Kyungpook coastal area were modified by HCI, NaOH and NH_4OH solutions and exposed to the atmosphere of various vapour pressure within the desiccator. Generally, silica gel exhibited as good moisture adsorbent at high humidity and as poor adsorbent at low humid atmosphere. However, moisture adsorption capacities of mordenite such as Na^+-K_111, NH_4^+-K_111, Na^+-Y_11 exceeded the silica gel at the lower moisture pressure than 16 torr. NH_4^+-MOrdenite proved itself to be best adsorbent in the closed system whose moisture pressure diminished as the time eclipsed. This system was most closely simulated to the working conditions of those commercial dryer in the package.

종합검진센터 내원자에서 관상동맥질환 위험요인들의 분포

이충원,이종영,박종원,윤능기,김영조,이현우,이무식,서석권 啓明大學校 醫科大學 1993 계명의대학술지 Vol.12 No.3

1991년3월부터 8월사이에 대구시에 소재한 1개의 대학병원의 종합검진센타에 내원해서 종합검진을 받은 남자 422명(69.9%)과 여자 182명(30.1%)을 대상으로 하여 심헐관계질환의 위험요인들의 전체적인 분포를 보았다. 평균 연령은 남자가 43.3(표준편차, 10.3), 여자가 44.4(표준편차, 10.8)였다. 남자에서 연령에 따라 0.05 수준에서 통계적인 유의성을 보인 위험인자는 혈청 총콜레스테롤(TC). 트리글리세라이드(TC), 고밀도 지단백콜레스테롤(HDL), 저밀도 지단백콜레스테롤(LDL), 수축기혈압, 비체중, A형행동양상이었으며 생활습관으로서는 음주, 흡연, 규칙적인 운동, 우유와 커피섭취, 수면 등이었다. 여자에서는 TC, TG, LDL, 수축기와 확장기혈압, 비체중 그리고 커피섭취와 수면 등이었다. 남자에서 총콜레스테롤의 평균은 181.7mg/dl(표준편차, 32.2), 여자는 182.5mg/이(표준편차, 42.2)였다. 대부분의 위험인자들은 구미의 수준에 미치지 못했으나 남자에서 흡연율이 전체적으로 61.8%로 높으 수준이었으며 특히, 20~29세가 72.9%, 30~39세가 75.2%로서 다른 연령군에 비해 상대적으로 높았다. 여자에서는 전체적으로 6.0%에 지나지 않았다. 확장기혈압 90mmHg 이상을 고혈압자로 정의했을 때의 유병률이 여자 전체대상자에서 24.7%로 높았으며 50-59세는 표본수가 적어서 문제가 되었으나 53.1%였다. 어떤 인구집단내에서 총콜레스테롤의 평균이 200mg/dl 미만이면 고혈압과 흡연의 인구집단의 수준에 관계없이 관상동맥질환(coronary heart disease)의 발생은 드문 것으로 보고가 되고 있으므로 당분간 관상동맥질환 발생률의 급속한 절대적인 증가는 힘드리라 사료된다. 그러나 이러한 결과는 본 연구의 대상자들이 대표성을 지니지 못하며 건강검진센터에 자발적으로 내원한 사람들이므로 해석에 주의를 요한다. Authors examined the distributions of the risk factors for the coronary heart disease in the 422 male (69.9%) and 182 female (30.1%) visitors to the health examination center of a university hospital located in Taegu March to August 1991. Mean age of males was 43.3(standard deviation, SD 10.3) and that of females was 44.4(SD 10.8). Total cholesterol(TC), triglyceride (TG), high-density lipoprotein and low-density lipoprotein cholesterol(LDL), systolic blood pressure, Quetelet index, and Type A Behavior Pattern, and some of life habit variables(alcohol intake, smoking, regular exercise, milk and coffee intake and sleeping) were statistically significant in age groups of the males(P<0.05). In the females, TC, TG and LDL, systolic and diastolic pressure, Quetelet index, coffee intake and sleeping were statistically significant in age groups (P<0.05). Mean of total cholesterol was 181.7㎎/dl(SD,32.2) in males and that of females was 182.5㎎/dl(SD,42.2). Most of the risk factors levels were lower than the Euro-Americans', while smokers were high with 61.8%, especially 72.9% in 20-29 age group and 75.2% in 30-39 age group in males. In females, smokers were just 6.0%. Hypertensives defined by more than 90mmHg diastolic pressure were 24.7% in females, particularly 53.1% in 50-59 age group, but size of the strata was rather small(N=49). In the light of the report that coronary heart disease is uncommon irrespective of population levels of smoking and hypertension, where average total blood cholesterol level in a population is low(<200mg/dl), it is not likely that the absolute increase of the number of the coronary heart disease will increase markedly in the near future. But cautions should be exercised in interpreting the results of this study due to the lack of representativeness and volunteerism. .

진행성 비호지킨 림프종 환자에서 ICE 구제 요법에 의한 조혈모세포 가동화 후 자가 말초혈액 조혈모세포이식

이제중,이병환,김여경,변정래,이일권,박무림,정익주,김형준 대한조혈모세포이식학회 2003 대한조혈모세포이식학회지 Vol.8 No.1

연구배경: 비호지킨 림프종에서 자가 말초혈액 조혈모세포이식술이 폭넓게 이용됨으로 인해서, 높은 치료효과뿐만 아니라 조혈모세포의 가동화 효율이 높은 구제 요법이 요구되었다. 방법: 본 연구에서는 진행성 비호지킨 림프종에서 ICE 요법을 이용하여 조혈모세포를 가동화시킨 후 자가 말초혈액 조혈모세포이식이 시행하였던 환자를 후향적으로 분석하였다. 결과: 대상 환자의 중앙 연령은 38세(범위, 16~61)였으며, ICE 요법은 환자당 4주기(범위, 1~6주기)가 투여되었고, 투여 간격은 중앙값이 24일(범위, 16~36일)이었다. 고위험도 관해군을 제외한 13예의 환자 중 완전반응은 7예(53.8%), 부분반응은 4예(30.8%), 진행성질환은 2예(15.4%)를 보였다. 치료에 따른 3~4등급의 혈액학적 독성은 중성구감소증이 13예(81.3%), 혈소판감소증이 7예(42.8%)에서 관찰되었다. 조혈모세포 채집은 ICE 요법 후 중앙값이 12일(범위, 6~20일)에 시행되었고, 각 ICE 요법당 채집한 단핵구치는 중앙값이 5.75× 10^(8)/체중, CD34^(+) 세포는 중앙값이 1.25× 10^(6)/체중, CFU-GM치는 중앙값이 1.19× 10^(5)/체중이었다. 조혈모세포이식은 11예에서 시행되었고, 중앙 추적기간 401일에 평가한 2년 전체생존율은 70.1±14.7%, 1년 및 2년 무사건생존율은 각각 60.7±15.4%와 32.3±17.1%를 보였다. 결론: ICE 요법은 재발성/불응성 비호지킨 림프종 환자에서 높은 치료 반응률과 만족할 만한 조혈모세포 가동화를 보여 주었지만, 치료에 대한 순응도가 낮아서 투여 간격이 연장되는 문제점을 안고 있어서, 우리나라 환자의 실정에 적합한 구제요법에 대한 연구가 필요할 것으로 생각된다. Background: Due to the extensive application of high-dose chemotherapy with autologous peripheral blood stem cell transplantation (PBSCT), a salvage chemotherapeutic regimen with high response rate as well as effective capacity of PBSC mobilization is needed in non-Hodgkin's lymphoma (NHL). Methods: We analyzed the applicability of ICE (ifosphamide, carboplatin, and etoposide) regimen in NHL patients who underewent autologous PBSCT. Results: The median age was 38 years (range, 16~61 years), the patients received median 4 cycles (range, 1~6 cycles) of ICE regimen, and the median interval between each chemotherapeutic cycle was 24 days (range, 16~36 days). There were 7 (53.8%) complete responses, 4 (30.8%) partial responses, and 2 (15.4%) progressive diseases after ICE regimen. Toxicity included grade 3/4 neutropenia and throm bocytopenia in 13 (81.3%) and 7 (42.8%) patients, respectively. PBSC collection began on median day 12 (range, 6~20 days) after ICE therapy. The median number of mononuclear cells, CD34+ cells, and CFU-GM was 5.75× 10^(8)/kg, 1.25× 10^(6)/kg, and 1.19× 10^(5)/kg, respectively. With a median follow- up of 401 days, the patients who underwent autologous PBSCT had overall survival with 70.1±14.7% at 2 year and event free survival with 60.7±15.4% and 32.3±17.1% at 1 year and 2 years, respectively. Conclusion: ICE chemotherapy is an effective cytoreduction and mobilization regimen in patients with NHL, but profound myelosuppression with delayed recovery might pose difficulties in applying for Korean patients. Further evaluation for appropriate salvage regimens in Korean patients should be needed.