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Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis
Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1
<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>
신규간호사와 프리셉터가 인지하는 프리셉터의 교수효율성 비교
김지양,김영선,김춘실,박현숙,신미영,윤연숙,조유숙,박미미,유문숙 한국간호과학회 간호행정학회 2009 간호행정학회지 Vol.15 No.2
Objective: The purpose of this study was to compare preceptors and new graduate nurses on their perception of preceptor teaching effectiveness. Methods: The participants were 90 new nurses and 90 preceptors who worked in A medical center. The data were collected from July 1 to September 30, 2008. A cross-sectional descriptive survey was done using a structured questionnaire. Results: New nurses' perception (4.07±.44) of the preceptor teaching effectiveness was significantly higher than perception of the preceptors (3.57±.37). Fifty five percent of new graduate nurses reported a lack of coherence in the preceptor's practice guidelines. For 5 causal factors classified on the teaching effectiveness, the factor of 'Professional knowledge and ability' showed the highest score, but 'Interpersonal and communication skill' got lowest score for both group. Preceptors responded that they did not have enough time to teach well because of their heavy workloads. Conclusions: These results suggest that the preceptors need appropriate compensation and education opportunities, and new graduate nurses need consistent education by the teaching professionals. Therefore, it is important to give preceptors full charge of the preceptorship. Also, it will be necessary to develop education programs to enhance interpersonal and communication skill for preceptors and new nurses.
도재브라켓의 제거방법에 따른 법랑질표면의 주사전자현미경학적 관찰
박미숙,윤영주,김광원 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.5
도재브라켓의 제거방법에 따라 법랑질표면에 미치는 영향을 비교 평가하기 위하여 80개의 발거된 소구치를 대상으로 통법에 의해 도재브라켓을 부착시키고 일주일 후 각각 40개의 기계적 방법에 의한 제거군과 전기열전도 방법에 의한 제거군으로 구분하여 도재브라켓의 제거를 유도한 후, 그 탈락양상 및 도재브라켓과 법랑질 표면의 주사전자현미경 관찰을 시행하였으며, 전기열전도 방법에 의한 제거군을 다시 각각 10개씩 고속 tungsten carbide bur에 의한 연마군, 저속 sof-lex disc에 의한 연마군, 고속 resin polishing bur에 의한 연마군, 그리고 초음파 치속제거기에 의한 연마군으로 분류하여 잔여레진의 연마를 시행한 후, 잔여레진의 연마를 시행한 후, 잔여레진의 평가에 의해 다음과 같은 결론을 얻었다. 1) 결찰와이어 절단용 겸자를 이용한 도재브라켓의 기계적 제거시, 0.69의 평균 잔여레진 부착지수를 보임으로써 법랑질과 레진 경계부에서 파절이 가장 빈발한 양상을 보였다. 2) 전기열전도 방법을 이용한 도재브라켓의 제거시 2.19의 평균 잔여레진 부착지수를 보임으로써 브라켓과 레진 경계부에서의 파절이 가장 빈발한 양상을 보였다. 3) 기계식 방법으로 도재브라켓의 제거시, 법랑질표면의 주사현미경 관찰소견은 실험군의 7.5%에서 법랑질표면의 탈락과 패임 등의 손상을 보였다. 4) 잔여레진의 제거의 고속 resin polishing bur 사용군에서 가장 적은 잔여레진막을 보였다. The purposes of this study were to evaluate and compare the frequency of ceramic bracket fracture, frequency of enamel fracture, bond fracture site, adhesive remnant index after mechanical and electrothermal debracketing, to evaluate effectiveness of high and low speed rotary instrument and ultrasonic instrument during residual adhesive remnants removal, and to measure resin film surface(percentage) using by image analyser(Leco 300). Bond fracture site, bracket fracture, and enamel surface damage were examined by scanning electron microscope. The following results were obtained: 1. In the mechanical debracketing group, the bond failed predominantly at enamel-adhesive interface with the bulk of adhesive remaining on bracket base. 2. In the electrothermal debracketing group, the bond failed predominantly at adhesive-bracket interface with the bulk of adhesive remaining on enamel surface. 3. The most effectiveness of residual resin removal was obtained by means of the resin polishing bur and the order of scratch formation was the precedure using tungsten carbide bur, ultrasonic scaler, sof-lex disc, and polishing bur. 4. The order of the resin film surface percentage was ultrasonic scaler, tungsten carbide bur, sof-lex disc, and resin polishing bur.
일부 大學敎授와 敎職員의 心血關係疾患 危險要因 및 健康行態에 關한 硏究
윤의성,천병철,박미숙,김순덕,염용태 고려대학교 의과대학 1997 고려대 의대 잡지 Vol.34 No.3
We reviewed the health screening record(1996) of a university to compare cardiovascular risk factors and major health behavior of teaching staffs with those of other staffs. The reviewed cardiovascular risk factors were body mass index (BMI), blood pressure (BP), total cholesterol (TCH) and fasting blood sugar (FBS). Smoking, drinking, exercise and diet habits were cheked as major risk factors. We defined the obesty (BMI>25), hypertension (systolic BP 140 or diastolic BP 90 mmHg), hypercholesterolemia (TCH 230mg/dl), and hyperglucosemia (FBS 110mg/dl) , drinking group (alcohol intake 3-4 times a week and over 1 bottle of soju a time), smoking group (smoking over 10 cigarettes a day), non-exercising group(do excercise below 3-4 times a week, and below 30 min a times), unbalanced diet group(do favor salty and spicy food or do favor flesh food). The 1,341(81.7%) of 1,640 staffs were screened in 1996, the teaching staffs were 614(69.3% of 886 teaching staffs) and other staffs (mainly clerks and administrators) were 737(97.7% of 754). 41 (6.7%) of teaching staffs were female and 166(22.4%) of the other staffs were. Among the teaching staffs, there were no below age 30 but 11.5% of them were over 60; in case of the other staffs 11.3% and 2.0% were in each age group. We divided the teaching staffs into medical staffs and non-medical staffs and compare the risk factors of each group. The total number of screened medical staffs were 92(36.2%) of 254, and non-medical staffs were 522 (82.6%) of 632. The results were follows; 1) The frequency of hypertension of each group were significantly different (7.2% in teaching staff, 11.8% in other staffs) after adjusting sex, age, smoking, drinking, exercise, and diet habits by multiple logistic regression (p<0.001). Hyperglucosemia were founded 10.5% and 12.4% in each staffs and the difference were significant after adjusting age, sex and health habits(p=0.005). Teaching staffs had more hypercholesterolemic persons than other staffs(24.3%, 16.3% in each staffs, p=0.019). Obesity frequency was not significantly different. 2) The number of drinking group were 114(18.6%), 208(28.3%) in each staffs, and this difference were significantly different (CMH=38.295, p=0.001). The number of smoker were 140(22.8%) and 274(37.2%) (CMH=58.109, p=0.001). The frequency of non-exercising group and the frequency of unbalanced diet group were not significantly different. 3) The frequency of obesity were 39.1% and 30.9% in each medical and non-medical staffs (CMH=4.414, p=0.036), but this difference remains non-significant after adjusting by the health habits. The frequency of hyperglucosemia of medical staffs (17.4%) were significantly higher than non-medical staffs (9.2%) after adjusting age, sex and the health habits (p=0.0005). Other risk factors were not significantly different. 4) Medical staffs had more smoker(29.4%) than non-medical staffs(21.5%) (CMH=6.401, p=0.011) and more non-exercising persons (27.2%) then non-medical staffs(11.1%) (CMH=12.446, p=0.001). Other health behaviors are not significantly different.
윤미향,정미선,박원혁 부산대학교 사범대학 과학교육연구소 2007 科學敎育硏究報 Vol.34 No.-
The purpose of this study was to find out the difference of decision-making process based on the nature of science. The contemporary and traditional point of view group regarding the nature of science were composed and provided socio-scientific issues. Also, the decision-making process questionaries and interview about socio-scientific issues were conducted to groups. The decision-making process questionary was consisted of five steps : identification of problem, searching alternatives, generation of alternatives, analysing alternatives, and selecting alternative. The results of this study showed at the both groups' decision-making process was difference in the first four steps: the one who has contemporary point of view recognized the various conflict situation, searched the objective informations, applied a diverse of standards, and found the concert solution but the other recognized conflict situation fragmentarily, searched the subjective informations, applied limited standards, and found the realizable solution. The result to the final step, selecting alternatives, were similar in both groups because they didn't suggest the rational reason for selecting alternatives. so, it request the long terms of decision-making experience to make rational decision regardless of the nature of science.