Effect of Ionizing Radiation on Rat Tissue: Proteomic and Biochemical Analysis

Park*, Eui‐,Chul,Yoon, Jong‐,Bok,Seong*, Jin‐,Sil,Choi, Kyoung‐,Soo,Kong, Eung‐,Sik,Kim, Yun‐,Jeong,Park, Young‐,Mee,Park, Eun‐,Mi Taylor Francis 2006 Preparative biochemistry & biotechnology Vol.36 No.1

<P>Reactive oxygen species (ROS), generated by ionizing radiation, has been implicated in its effect on living tissues. We confirmed the changes in the oxidative stress markers upon irradiation. We characterized the changes in the proteome profile in rat liver after administering irradiation, and the affected proteins were identified by MALDI-TOF-MS and ESI-MS/MS. The identified proteins represent diverse sets of proteins participating in the cellular metabolism. Our results demonstrated that proteomics analysis is a useful method for characterization of a global proteome change caused by ionizing radiation to unravel the molecular mechanisms involved in the cellular responses to ionizing radiation.</P>

월악산국립공원의 곤충다양성에 관한 연구 - 노린재목, 딱정벌레목, 나비목 -

박성준(Seong-Joon Park),전용락(Yong-Lak Jeon),홍의정(Eui-Jung Hong),윤주창(Ju-Chang Yun),박서경(Seo-Kyoung Park),김병진(Byung-Jin Kim) 국립공원연구원 2010 국립공원연구지 Vol.1 No.4

본 연구는 2007년부터 2009년까지 3년 동안 월악산 모니터링 조사 지점과 동일지구에 대해 정기적인 주 간조사를 통해 주간곤충류(노린재목, 딱정벌레목, 나비목)의 종목록을 확보 하여, 월악산국립공원의 생태계에 자연적 혹은 인위적으로 변화된 내용을 측정하여 곤충자원의 체계적인 관리를 위한 자료를 제공하고자 실시되었다. 본 조 사기간 동안 채집 확인된 주간곤충류는 65과 404종(노린재목 21과 86종, 딱정벌레목 38과 244종, 나비목 6과 74종) 이 조사되었다. 따라서 문헌조사를 포함한 결과 월악산국립공원에 서식하는 것으로 확인된 주간곤충류는 멸종위기야 생동물 2종, 고유종 6종, 국외반출승인대상종 10종과 특정종 50종을 포함하여 총 72과 609종(딱정벌레목 44과 368 종, 노린재목 22과 126종, 나비목 6과 115종)으로 나타났다. 아울러 조사지점별 분석결과 S3에서 종다양성이 가장 높게 나타났으며, S10은 다른 조사지점과 다르게 종다양성이 현저하게 떨어지는 것으로 나타났다. 각 조사기간별 우점종으로는 2007년과 2009년도에 넉점박이송장벌레, 2008년도에는 더듬이긴노린재로 나타났다. This study were conducted for three years from 2007 to 2009 and at the same points as monitoring surveys, through regular surveys insects (Hemiptera, Coleoptera, Lepidoptera) to obtain the list, to measure and assess  Woraksan National Park ecosystem’s natural or artificial change, to provide data for systematic management of the contents of insects. As a result of all the collections were examined, 404 species of 65 family under 3 order were identified. Therefore, the species composition of insect over each result from Woraksan National Park’s was a total of 3 orders,  72 families, 609 species including 2 Endangered species, 6 Endermic species, 10 Management of exotic species and 50 Designated species; 368 species of family Coleoptera which is the highest, 126 species of family Hemiptera and 115 species of family Lepidoptera. Site 3 had the highest indices diversity. In contrast, Site 10 indices diversity, different  from the other survey points, were significantly low. In each survey period, dominant species included Nicrophorus quadripunctatus in 2007 and 2009. In 2008, dominant species was Pachygrontha antennata.

Bupivacaine과 ropivacaine이 Xenopus oocyte에 발현된 HERG 전류에 미치는 영향

김국성,이규승,김효신,손숙진,이상도,김광진,전병화,김윤희,박진봉 충남대학교 의과대학 의학연구소 2003 충남의대잡지 Vol.30 No.1

Bupivacaine is an amide-type local anesthetic widely used for regional anesthesia. Ropivacaine is developed as a less cardiotoxic alternatives to bupivacaine. In the present study, we have analyzed the effects of bupivacaine and ropivacaine on HERG currents expressed in Xenopus oocytes. Bupivacaine and ropivacaine(3∼1,000μM) blocked HERG currents in a concentration dependent manner. EC_(50) was 26.1±3.1μM(n_(R) 0.65±0.04) and 43.5±7.9μM(n_(H) 0.99±0.13) in bupivacaine and ropivacaine, respectively. Bupivacaine and ropivacaine did not affect the activation and deactivation kinetics of HERG channels. However, the drugs decreased the slope conductance measured from fully activated current-voltage relationship curves. These results suggest that bupivacaine and ropivacaine have a similarinhibitory effect on HERG channels, which could be a possible cellular mechanism of LQT or ventricular arrythmia by the drugs.

정신보건관련시설 입원(소)자의 주요 정신장애 유병률 : 전국 20개 시설의 입원(소)자를 대상으로

조성진,조맹제,서동우,함봉진,홍진표,배재남,이준영,이동우,박종익,전홍진,김성주,김용익 大韓神經精神醫學會 2004 신경정신의학 Vol.43 No.6

Objectives : One of the objectives of this study is to estimate the prevalence rates of psychiatric diagnoses in the combined populations of psychiatric hospitals, psychiatric nursing facilities and homeless asylums using the Korean version of the Composite International Diagnostic Interview (K-CIDI), which is a comprehensive and fully standardized interview schedule to assess psychiatric disorders for diagnosis. The Other objective is to compare with previously studied prevalence rates of Psy-chiatric diagnoses using the results of this study. Methods : The study subjects, aged from 181o 64 years, were randomly selected from 64,582 institutionalized population of psychiatric hospitals, Psychiatric nursing facilities and homeless asylums as of 30, June, 2001. Twelve trained interviewers administered the K-CIDI to the selected respondents. A total of 1,875 respondents (male 1,194, female 681) completed the interview. Results : The lifetime and one year prevalences of any diagnosis excluding nicotine dependence · withdrawal, anxiety disorder, eating disorder, somatoform disorder were 88.2% (male 88.1%, female 88.3%), and 65.8% (male 62.7%, female 73.7%), respectively. The lifetime and one year prevalences of schizophrenia and other psychotic disorders were 78.5% (male 75.3%, female 83.7%), and 56.8% (male 51.9%, female 64.8%), respectively. The lifetime and one year prevalences of alcohol use disorder (dependence/abuse) were 26.7% (male 37.9%, female 8.3%), and 8.7% (male 12.8%, female 2.1%), respectively. The lifetime and one year prevalences of mood disorder were 18.1% (male 13.5%, female 25.8%), and 10.4% (male 7.2%,female 16.9%), respectively. The lifetime and one year prevalences of schizophrenia and other psychotic disorders were comparable with the 2001 community survey by 1.19% to 1.32%, and 0.51% to 0.61%, respectively. The lifetime and one year prevalences of schizophrenia were also comparable by 0.16% to 0.28%, and 0.16% to 0.25%, respectively. There were no significant changes of prevalence rates when correction were applied to other psychiatric diagnosis. Conclusion : The results of this study could be used for evaluating the distribution of psychiatric diagnoses in mental health related facilities and for planning mental health policies.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

월악산국립공원의 곤충다양성에 관한 연구 : 노린재목, 딱정벌레목, 나비목

박성준(Seong-Joon Park),전용락(Yong-Lak Jeon),홍의정(Eui-Jung Hong),윤주창(Ju-Chang Yun),박서경(Seo-Kyoung Park),김병진(Byung-Jin Kim) 국립공원연구원 2010 국립공원연구지 Vol.1 No.3

본 연구는 2007년부터 2009년까지 3년 동안 월악산 모니터링 조사 지점과 동일지구에 대해 정기적인 주간조사를 통해 주간곤충류(노린재목, 딱정벌레목, 나비목)의 종목록을 확보 하여, 월악산국립공원의 생태계에 자연적 혹은 인위적으로 변화된 내용을 측정하여 곤충자원의 체계적인 관리를 위한 자료를 제공하고자 실시되었다. 본 조사기간 동안 채집 확인된 주간곤충류는 65과 404종(노린재목 21과 86종, 딱정벌레목 38과 244종, 나비목 6과 74종)이 조사되었다. 따라서 문헌조사를 포함한 결과 월악산국립공원에 서식하는 것으로 확인된 주간곤충류는 멸종위기야생동물 2종, 고유종 6종, 국외반출승인대상종 10종과 특정종 50종을 포함하여 총 72과 609종(딱정벌레목 44과 368종, 노린재목 22과 126종, 나비목 6과 115종)으로 나타났다. 아울러 조사지점별 분석결과 S3에서 종다양성이 가장 높게 나타났으며, S10은 다른 조사지점과 다르게 종다양성이 현저하게 떨어지는 것으로 나타났다. 각 조사기간별우점종으로는 2007년과 2009년도에 넉점박이송장벌레, 2008년도에는 더듬이긴노린재로 나타났다. This study were conducted for three years from 2007 to 2009 and at the same points as monitoring surveys, through regular surveys insects (Hemiptera, Coleoptera, Lepidoptera) to obtain the list, to measure and assess Woraksan National Park ecosystem’s natural or artificial change, to provide data for systematic management of the contents of insects. As a result of all the collections were examined, 404 species of 65 family under 3 order were identified. Therefore, the species composition of insect over each result from Woraksan National Park’s was a total of 3 orders, 72 families, 609 species including 2 Endangered species, 6 Endermic species, 10 Management of exotic species and 50 Designated species; 368 species of family Coleoptera which is the highest, 126 species of family Hemiptera and 115 species of family Lepidoptera. Site 3 had the highest indices diversity. In contrast, Site 10 indices diversity, different from the other survey points, were significantly low. In each survey period, dominant species included Nicrophorus quadripunctatus in 2007 and 2009. In 2008, dominant species was Pachygrontha antennata.

폐결핵 환자 혈청에서 수용성 Interleukin 2 수용체 및 Adenosine Deaminase 활성도에 관한 연구

고정희,박성규,백상현,박찬권,박병수,안진영,최우석,박정규 충남대학교 의과대학 지역사회의학연구소 1993 충남의대잡지 Vol.20 No.1

Interleukin-2 (IL-2) induces T cell proliferation in an autocrine manner and provides a means by which antigen triggered T cells can be clonally expanded in vitro. During the following activation, the activity of IL-2 is mediated by specific high affinity IL-2 binding membrane receptors which are expressed shortly after activation. In this process, a 42 KD-fragment (soluble IL-2R) is continuously cleaved off and circulates as a soluble marker of T lymphocyte activation. Elevated level of soluble IL-2R has been identified in the serum of patients with malignant autoimmune and allergic disorders, systemic parasitic infection, undergoing graft versus host disease, acute or chronic lymphocytic leukemia and HIV-infection. ADA (adenosine deaminase) completes the process of differentiation of T cell and is essential for progression of T cell maturation. Therefore level of ADA is to correlated with magnitude of T cell immune response. The fact that expression of sIL-2R and ADA activity increases in peripheral blood T-lymphocytes of patients with active pulmonary tuberculosis suggests that T cell activation might have a major role in the pathogenesis of pulmonary tuberculosis. In order to evaluate the T cell immune response in pulmonary tuberculosis, we measured the serum concentration of sIL-2R and ADA activity in 17 patients with current pulmonary tuberculosis, 10 chronic inactive pulmonary tuberculosis, 10 as normal controls. (1) Current pulmonary tuberculosis had significantly higher levels of sIL-2R (237.24±95.47)when compared with those of inactive tuberculosis (78.6±11.06). and the control (68.17±15.4) group. (2) ADA activity in current pulmonary tuberculosis was significantly increased (34.41±20.63) when compared with those of inactive tuberculosis (24.7±14.36) and control (17.65± 5.94) group. (3) There was good correlation between sIL-2R concentration and ADA activity in serum in current pulmonary tuberculosis group. (4) sIL-2R concentration and ADA activity was decreased significantly 6 months after anti-tuberculosis drug medication. In conclusion, sIL-2R concentration and ADA activity in serum in current pulmonary tuberculosis group was increased when compared with those of inactive pulmonary tuberculosis and the control group. By the way, T cell mediated immune response was enhanced in current pulmonary tuberculosis, but in inactive chronic pulmonary tuberculosis who had treated by antituberculosis drug medication, the concentration of sIL-2R and ADA activity was nearly normal.

NSIP와 BOOP의 고해상전산화단층촬영 소견과 비교

백상현,박재성,차장규,홍현숙,김대호,박성진,조준희,이혜경,최진수 순천향의학연구소 2003 Journal of Soonchunhyang Medical Science Vol.9 No.2

Purpose: BOOP and NSIP also share similar clinical features such as subacute flu-like illness, the finding of bronchoalveolar lavage, the result of pulmonary function test and more favourable outcome than usual interstitial pneumonia(UIP). The author try to compare the HRCT findings of NSIP with BOOP by pattern and distribution of diseases. Materials and Methods: HRCT findings of histopathologically proven NSIP(Group Ⅰ; 6, Group Ⅱ; 7, Group Ⅲ; 2) in 15 patients(5 male, 10 female, age range, 39-69 years; mean age, 53 years), BOOP in 15 patients(6 male, 9 female, age range, 26-76 years; mean age, 52 years) were reviewed retrospectively. Results: The common pattern of the two diseases was admixed consolidation, ground glass attenuation & reticular densities with/without architectural distortion(NSIP; 53.3%, BOOP; 46.7%). The main component of the pattern wan predominantly consolidation(NSIP; 46.7%, BOOP; 73.3%) and ground glass attenuation(NSIP; 40.0%, BOOP;20.0%). Architectural distotion was more provinent finding with NSIP(mild ; 3, moderate ; 4, severe; 2) compared with BOOP(mild; 7, moderate; 2, severe; 0). The predominant overall distribution was lower lung, peribronchovascular & peripheral lung field in both of the two entities(NSIP;73.3%, BOOP; 73.3%). Peribronchovascular involvement was more common with NSIP(100%) than BOOP(80%), whereas peripheral involvement was more common with BOOP(93.3%) than NSIP(80%). Conclusion: The HRCT findings of NSIP and BOOP were similar in pattern and distribution. But NIP manifested more frequently ground-glass attenuation, whereas BOOP showed more frequently consolidation. Architectural distortion was more severe with NSIP than BOOP. NSIP invoved more frequently peribronchovascular space and BOOP were more common in peripheral lung field.

지상라이다를 이용한 미기록 외래종 갯쥐꼬리풀(Spartina Alterniflora)의 분포특성과 관리방안 연구 -다도해 해상국립공원 진도 남동리 해안을 사례로-

박정원 ( Jung Won Park ),김하송 ( Ha Song Kim ),장성건 ( Seong Geon Jang ),천숙진 ( Sook Jin Chun ),육관수 ( Kwan Soo Yuk ) 한국도서학회 2015 韓國島嶼硏究 Vol.27 No.3

갯쥐꼬리풀(Spartina alterniflora)은 북미 대서양 연안 뉴펀들랜드, 캐나다 남쪽에서 아르헨티나 북부의 갯벌이 원산이며, 생장이 강한 특성으로 세계의 기수지역과 해안에서 생태공학적 용도로 도입된 바 있다. 그러나 매우 빠르게 갯벌을 피복하여 자생종의 생장을 방해하고, 염습지 종다양성을 감소 시켰고, 결과적으로 과다생육으로 갯벌 무척추동물의 서식지를 잠식해 사라지게 하여 무척추동물을 먹이로 하는 조류 또한 생태계 내에서 감소키는 등 많은 환경문제를 야기 시켰다. 우리나라에서는 2008년 전후에 다도해해상국립공원에 유입된 것으로 판단된다. 현재 다도해해상국립공원 남동리 해안에서 분포하고 있는 갯쥐꼬리풀(Spartina alterniflora)군락은 습지내 총 6개의 염생식물 군락면적(8,487.7㎡) 중 75.34%의 비율(6,394.86㎡)로 분포하고 있다. 또한 2008년에 11.54㎡ 이던 분포 면적이 2015년 현재 약 550배가 늘어난 6,394.22㎡로 조사되었고 생육지 고도 범위인 0.69m~1.1m를 요인 값으로 두고 GIS 분석한 하였다. 그 결과 앞으로 확산 될 예상 면적은 12,584㎡이며 현재보다 약 6,189.14㎡가 늘어날 것으로 예측되며 우리나라에서도 역시 확산이 빠르고 넓게 늘어날 것으로 판단된다. 향후 계속 분포역이 확대 될 것으로 예상하고 관리방안을 위하여 시범 연구를 실시하였다. 갯쥐꼬리풀군락 중 일부를 첫 번째는 뿌리째 뽑아내기와 두 번째 지상부 제거 후 포장덮기 방법 등 국외사례의 문헌을 바탕으로 방재를 실시하였으나 전체 면적을 방재하기에는 많은 인력과 시간 그리고 예산이 소요되므로 다각적인 방안을 강구하여야 한다. Spartina alterniflora is native to the Atlantic coast from Newfoundland, the south of Canada and northern Argentina. There it forms a dominant part of brackish coastal saltmarshes. Due to its strong growth, the Spartina alternifolra was introduced to the rider area and coast as a use for ecological engineering. The Spartina alterniflora community can crowd out native species, reduce the biodiversity of saltmarsh and alter the environment. As a result, the invertebrates living in mud flats disappeared due to the encroaching of Spartina alterniflora, and the birds which eat off invertebrates went along as well. The Spartina alterniflora was first introduced in South Korea in 2008 and is known to have flowed in the Dadohaehaesang National Park. Compared to 2008, in 2015 the distribution area of the Spartina alterniflora has increased 550 times. (11.54㎡ in 2008 to 6,394.22㎡ in 2015). The large increase of Spartina alterniflora calls for urgent management and additionary study on the subject. Thus, this study analyzed the geo-spatial information on the Spartina alterniflora such as its distribution area, the height of habitats and expected expansion area, and can act as the preliminary data for management.

대량의 각혈로 사망한 장티푸스 1예

한상우,유진홍,권순석,김성훈,박지찬,홍은정,최경성,박용완,장준희,안지원,박유경,강지영,박상미,신완식 대한감염학회 2004 감염과 화학요법 Vol.36 No.5

We experienced a 25 year-old male patient with typhoid fever complicated with massive hemoptysis. Pulmonary complication in typhoid fever is very rare and to our knowledge, there has been no report of hemoptysis as a main cause of death with this disease. We herein report a rare case of typhoid fever.