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Effect of Temperature on the Killing of Opisthorchis viverrini Eggs In Vitro
Parichart Boueroy,Kunyarat Duenngai,Chatanun Eamudomkarn,Panupan Sripan,Thidarut Boonmars,Benjamabhorn Pumhirunroj,Atchara Artchayasawat,Jiraporn Songsri,Kanpicha Chomphumee,Panaratana Rattanasuwan,Po 대한기생충학ㆍ열대의학회 2019 The Korean Journal of Parasitology Vol.57 No.1
Contaminated liver fluke egg in the environment has led to the high prevalence of human opisthorchiasis asso- ciated with cholangiocarcinoma in Southeast Asia. To find the effective lessening methods of Opisthorchis viverrini eggs in the contaminated environment, we investigated the temperature conditions for killing of these trematode eggs in vitro. Numerous O. viverrini eggs were obtained in the proximal part of uteri of adult worms from experimental hamsters. Ma- ture eggs with miracidium were allocated by experimental groups (2 control: positive and negative and 4 treatment: 50, 60, 70, and 80°C) with 0.85% saline, and treated by the experimental plan. Eggs in each experimental groups were ob- served under the confocal microscope after stain with Propidium Iodide (PI) to evaluate the effect of temperatures. Eggs in 70 and 80°C groups were all killed after over 10 min heated. Majority of eggs in 60°C (10, 15, and 30 min heated), 70 and 80°C (5 min heated) groups were inactivated. However in 50°C group, below half of eggs were to be killed in all time lapse (10, 15 and 30 min). In order to prevent O. viverrini infection and cholangiocarcinoma, direct treatment of sewage by heating at 70 or 80°C at least 10 min is essential. Therefore, treatment of O. viverrini eggs at a high temperature is a po- tential method for controlling egg contamination in sewage.
Current Status and Breeding Perspectives of Major Vegetable Crops in Thailand
김민경,장정희,Parichart Potchanasin,채원병,유은하,권택윤 한국국제농업개발학회 2019 韓國國際農業開發學會誌 Vol.31 No.1
The climate of Thailand is appropriate for year-round production of vegetables whose domestic market share has continuously increased and production contributes significantly to the export. However, technology of vegetable breeding and cultivation is still in its early stage of development, compared to developed countries. Information on the major crops and their leading cultivars or varieties is prerequisite for establishing appropriate breeding strategies and developing cultivation technologies for vegetables. This article provides the current state of five major vegetables in Thailand such as chili (Capsicum annuum L. and C. frutescens L.), cabbage (Brassica oleracea L. var. capitata), shallot (Allium ascalonicum L.), yard long bean (Vigna sesquipedalis L.) and lettuce (Lactuca sativa L.), with their leading cultivars or varieties and traits that consumers in Thailand prefer. Breeding perspectives of these major vegetables in Thailand were also discussed.
Theerak Winakon,Ditthakit Parichart,Puttamuk Thamrongjet,Chuawong Pitak 한국농업기계학회 2023 바이오시스템공학 Vol.48 No.4
Purpose The use of photocatalytic titanium dioxide ( TiO2) nanoparticles (NPs) in agriculture has been considered a potential framework for increasing crop yields. However, studies on the usage of TiO2 NPs for rice (Oryza sativa) production showed disparities in the effects of TiO2. The objectives of this study are to elucidate the effects of TiO2 and to evaluate the use of TiO2 NP for rice production in Thailand, by investigating the influence of TiO2 NP sizes and quantities on the early development of rice. Methods Seeds of two rice cultivars (cv. Thai Pathum Thani Fragrant and Thai Jasmine 105) were exposed to TiO2 suspensions of varying diameters and concentrations and transferred to Petri plates for early growth observation. The morphology of TiO2 was characterized by electron microscopy (EM) and dynamic light scattering (DLS). The accumulation of TiO2 was characterized by inductively coupled plasma mass spectrometry (ICP-MS). Results TiO2 exposure had negligible impacts on seed germination but positively affected seedling height, fresh and dry weight, and survival rate. Four hundred ninety-nanometer TiO2 NPs provided the best growth improvement compared to 100-nm and 23-nm NPs. Higher concentrations, up to 1000 mg/L, of TiO2 led to greater seedling height and weight. The increase in seedling growth quantities also correlated to TiO2 bioaccumulation and chlorophyll content in rice leaves, suggesting photosynthesis promotion as a possible mechanism for growth enhancement. Conclusion Four hundred ninety-nanometer TiO2 NPs improve the early stage of rice growth more than smaller-sized TiO2 NPs that significantly agglomerate in water. Higher concentrations, up to 1000 mg/L, further enhance the growth.
박민영,김찬성,박동주,Parichart Pattanamekar,김은미 대한교통학회 2010 大韓交通學會誌 Vol.28 No.1
The study developed physical distribution channel choice models reflecting decision making of the firms and studied how choice decision factors influence selection of distribution channel. The distribution channel survey data in Korea was used to do empirical study. As a choice set, distribution channels were classified into two main choice channels: direct and indirect channels. In addition, indirect channels were classified into other three channels according to the type of intermediate point: distribution center, wholesale store, and agency. This study developed choice models by applying both binary and multinomial logit model with various set of factors. The results showed that the developed logit models seemingly reflect distribution channel choice behaviors. The hypothesis tests on how each factor influences choice of distribution channel were performed and discussed as well. 최근 국내에서는 민간 분야뿐만 아니라 공공물류에의 활용을 위해 좀 더 신뢰성 있고 개선된 화물수요추정방법에 대한 관심이 높아지고 있다. 현재 화물수요분석의 기초자료로 활용되고 있는 지역 간 화물기종점통행량(OD)자료는 각 화물의 생산지와 소비지 사이의 이동과 전환관계가 복잡함에도 불구하고 출발지와 목적지 사이의 환적시설이나 물류시설을 노드로 구분하지 않고 각 존의 물동량에 포함시킴으로써 화물의 정확한 이동이나 전환관계를 파악하지 못하게 하는 한계점을 지니고 있다. 본 연구의 목적은 이러한 인식하에 개별의사결정자인 기업에 의해 이동되는 화물의 물적인 흐름을 분석하기 위한 물적 유통경로 선택모형을 개발하는 것이다. 이는 개별회사의 의사결정에 있어 매우 거시적인 개념이며, 개별의사결정자인 기업이 유통경로를 선택할 때 어떠한 요소를 고려하는 지를 살펴보고자 하였다. 본 연구에서는 2007 화물유통경로조사의 내수화물자료를 중심으로 기초통계분석을 수행하고 물적 유통경로 선택모형을 구축하였다. 또한 구축된 모형이 합리적인지 평가하기 위해 최종모형에 대해 유통경로선택이론을 기초로 가설검증을 수행하고 적중률을 산출하였다. 선정된 최종모형의 가설 검증결과, 제조업체는 유통경로선택시 도착지점의 시장특성, 업종 및 품목특성, 경로특성, 기업의 크기 및 경제력 등을 중요하게 고려하는 것으로 나타났다.
Kaewpitoon, Soraya J,Rujirakul, Ratana,Wakkuwattapong, Parichart,Matrakool, Likit,Tongtawee, Taweesak,Panpimanmas, Sukij,Pengsaa, Prasit,Jomkoa, Darawan,Joosiri, Apinya,Kaewpitoon, Natthawut Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.6
Opisthorchis viverrini is still a serious problem in Northeastern and Northern Thailand. Active surveillance is required to determine updated data for further prevention and control planning. Therefore, this study aimed to examine the prevalence and risk factors for O. viverrini in three provinces, Northeastern Thailand. A cross-sectional survey was conducted during October 2015 to March 2016 at Kaeng Sanam Nang district of Nakhon Ratchasima province, Waeng Noi district of Khon Kaen province, and Khon Sawan district of Chaiyaphum province, Thailand. Stool samples were examined by using a modified Kato-Katz Thick smear technique. From a total of 978 participants screened, O. viverrini infection was found in 1.74%, the majority opf positive cases being male (6.62%), age group 51-60 years old (4.21%), educated at primary school (8.43%), occupied with agriculture (9.62%),having an income <4,000 baht per month (4.82%), and living in Khon Sawan district (8.43%). Participants had a high knowledge level (42.43%), good attitude level (34.76%), and fair level (38.04%). The present study indicates the O. viverrini infection rate is low, but elderly males with primary school education involved in agriculture are still frequently effected particularly in Khon Sawan district. Therefore, this risk group requires behavior modification and continued monitoring.
Kaewpitoon, Soraya J,Rujirakul, Ratana,Wakkuwattapong, Parichart,Matrakool, Likit,Tongtawee, Taweesak,Norkaew, Jun,Kujapun, Jirawoot,Kampangsri, Wilas,Kaewpitoon, Natthawut Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.4
Background: Liver fluke infection is associated with cholangiocarcinoma; the bile duct cancer found frequently in the northeast and north of Thailand. Prevention and control particularly requires health education and behavior change. Objective: This study aimed to improve health behavior among village health volunteers (VHV) regarding liver fluke exposure in an epidemic area. Materials and Methods: A quasi-experimental study was performed during July 2015 to January 2016 in Sang Kha district of Surin province, Thailand. A total of 67 VHVs underwent a health education program (HEP) and data were collected on knowledge, attitude, and practice (KAP) before and after participation for HEP 3 months with a pre-designed questionnaire. The Students paired T-test was used for comparisons of mean KAP levels before/after the intervention. Results: The results revealed that knowledge (P-value=0.004), attitude (P-value=0.004), and practice level (P-value=0.000) were significantly improved after participation in the HBP. Attitude was significantly associated with knowledge (r=0.266, p<0.05), and practice (r=0.348, p<0.01). Conclusions: The implementation of health education among VHVs is feasible and increases their KAP. This improvement should have potential in liver fluke prevention and control in local communities in rural Thailand.
Kaewpitoon, Soraya J,Rujirakul, Ratana,Wakkuwattapong, Parichart,Benjaoran, Fuangfa,Norkaew, Jun,Kujapun, Jirawoot,Ponphimai, Sukanya,Chavenkun, Wasugree,Kompor, Porntip,Padchasuwan, Natnapa,Kaewpitoo Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.6
A quasi-experimental study was conducted to develop a health education modification program based on self-efficacy and motivation regarding liver flukes and cholangiocarcinoma development in Keang Sanam Nang district, Nakhon Ratchasima province, Thailand. A total of 36 individuals were invited to participate in the program and were screened for population at risk of liver fluke infection and cholangiocarcinoma using SUT-OV-001 and SUT-CCA-001. Development of health education modification program regarding liver fluke and cholangiocarcinoma prevention included 3 steps: (1) preparation, (2) health education program, and (3) follow-up and evaluation. The study was implemented for 10 weeks. Pre-and-post-test knowledge was measured with questionnaires, Kuder-Richardson-20: KR-20 = 0.718,and Cronbach's Alpha Coefficient = 0.724 and 0.716 for percection and outcome expectation questionnaires. Paired and independent t-tests were applied for data analysis. The majority of the participants were female (55.6%), aged between ${\leq}50$ and 60 years old (36.1%), married (86.1%), education level of primary school (63.9%), agricultural occupation (80.6%), and income <4,000 Baht (44.4%). The results revealed that after the health education program, the experimental group had a mean score of knowledge, perception, and outcome expectation regarding liver fluke and cholangiocarcinoma prevention significantly higher than before participation and in the control group. In conclusion, this successful health education modification program for liver fluke and cholangiocarcinoma, therefore may useful for further work behavior modification in other epidemic areas.