Fermented Barley Supplementation Modulates the Expression of Hypothalamic Genes and Reduces Energy Intake and Weight Gain in Rats

PB Tirupathi Pichiah,조석호,한성규,차연수 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.4

Dietary fiber and proteins are individually known to decrease feeding, but could result greater weight management benefit when both are combined. We hypothesized that supplementing the diet with fermented barley, being rich in both dietary fiber and proteins, could lower energy intake by modulating the mRNA expression level of hypothalamic genes associated with the regulation of feeding behavior and satiety; thereby decreasing body weight gain. To test our hypothesis, four groups of Sprague Dawley rats were arranged in a 2 × 2 factorial design (n = 6), low-fat diet with either guar gum (LFD-G) or fermented barley (LFD-FB) and high-fat diet with either guar gum (HFD-G) or fermented barley (HFD-FB). Using oral gavage, fermented barley was given at a dosage of 1500 mg/kg body weight and guar gum was supplemented in an equivalent quantity to that of the fiber in the fermented barley. After 19 weeks, the fermented barley-supplemented groups showed a significant reduction in energy intake, triglyceride, body weight gain, and serum leptin, compared to the guar gum-supplemented groups in both the low- and high-fat diet groups. Likewise, the anorexigenic gene proopiomelanocortin (POMC) and cocaine and amphetamine-regulated transcript (CART) mRNA level were significantly higher in the fermented barley-supplemented groups compared to the guar gum-supplemented groups in rats fed on both high- and low-fat diets. In conclusion, fermented barley supplementation upregulated hypothalamic POMC/CART, decreased energy intake in both low- and high-fat diet groups, and prevented excessive weight gain in rats.

<i>Salicornia herbacea</i> prevents weight gain and hepatic lipid accumulation in obese ICR mice fed a high‐fat diet

Pichiah, PB Tirupathi,Cha, Youn‐,Soo John Wiley Sons, Ltd 2015 Journal of the Science of Food and Agriculture Vol.95 No.15

<P><B>Abstract</B></P><P><B>BACKGROUND</B></P><P>Foods that are rich in fat and or sodium chloride promote obesity and associated diseases, whereas intake of dietary fiber averts obesity development. <I>Salicornia herbacea</I> (SH) is a rich source of dietary fiber and high in sodium chloride; therefore, we investigated whether replacing common salt with SH in a high‐fat diet could prevent obesity development.</P><P><B>RESULTS</B></P><P>Mice were divided into five groups: group ND was fed a normal diet, group HD was fed a high‐fat diet, group HD‐NaCl was fed a high fat diet with sodium chloride 10 g kg<SUP>−1</SUP>, group HD‐CL was fed a high‐fat diet with cellulose 30 g kg<SUP>−1</SUP> and group HD‐SH was fed a high‐fat diet with SH powder 50 g kg<SUP>−1</SUP>. The amount of sodium chloride and cellulose added in the respective diet was equivalent to their amount in SH. Data from our study showed that, SH supplementation significantly decreased body weight gain, liver weight, hepatic triglyceride, serum leptin and insulin, along with the mRNA level of key lipid anabolic genes such as SREBP‐1c, PPARγ and FAS compared to the HD group.</P><P><B>CONCLUSION</B></P><P>The results of this study demonstrated that SH is a potential natural anti‐obesity agent that can be used in place of sodium chloride. © 2014 Society of Chemical Industry</P>

Association of Poly (ADP-Ribose) Polymerase 1 Variants with Oral Squamous Cell Carcinoma Susceptibility in a South Indian Population

Anil, Sukumaran,Gopikrishnan, PB,Basheer, Ashik Bin,Vidyullatha, BG,Alogaibi, Yahya A,Chalisserry, Elna P,Javed, Fawad,Dalati, MHN,Vellappally, Sajith,Hashem, Mohamed Ibrahim,Divakar, Darshan Devang Asian Pacific Journal of Cancer Prevention 2016 Asian Pacific journal of cancer prevention Vol.17 No.8

Background: Oral cancers account for approximately 2% of all cancers diagnosed each year; however, the vast majority (80%) of the affected individuals are smokers whose risk of developing a lesion is five to nine times greater than that of non-smokers. Tobacco smoke contains numerous carcinogens that cause DNA damage, including oxidative lesions that are removed effectively by the base-excision repair (BER) pathway, in which poly (ADP-ribose) polymerase 1 (PARP-1), plays key roles. Genetic variations in the genes encoding DNA repair enzymes may alter their functions. Several studies reported mixed effects on the association between PARP-1 variants and the risk of cancer development. Till now no reported studies have investigated the association between PARP-1 variants and oral squamous cell carcinoma (OSCC) risk in an Indian population. Materials and Methods: In the present case control study 100 OSCC patients and 100 matched controls were genotyped using PARP1 single nucleotide peptides (SNP's) rs1136410 and rs3219090 using TaqMan assays. Results: The results indicated significantly higher risk with PARP1 rs1136410 minor allele "C" (OR=1.909; p=0.02942; CI, 1.060-3.439). SNP rs1136410 also showed significantly increased risk in patients with smoking habit at C/C genotype and at minor allele C. Conclusions: The PAPR-1 Ala762Val polymorphism may play a role in progression of OSCC. Larger studies with a greater number of samples are needed to verify these findings.

CHRONIC HYPOKALEMIA PROMOTES DIFFEPENTLLAL EXPRESSION OF THE GASTRIC H/K -ATR ase A SUBUNIT IN RAT KIDNEY

Ahn,KY,Turner,PB,Madsen,KM,Kone 대한신장학회 1995 춘계학술대회 초록집 Vol.1995 No.-

< 구두-E-13 > Potential of mineral oil migration from recycle paper based packaging into food

( Lisman Suryanegara Fitria ),( Dede Hy Yanto ),( Raden Pb Laksana ),( Yudhi D Kurniawan ),( Widya Fatriasari ),( Euis Hermiati ) 한국목재공학회 2018 한국목재공학회 학술발표논문집 Vol.2018 No.1

Food packaging is an important part in supporting food safety rules. The fact that not all of food packaging materials meet the requirements of food packaging materials, even primary, secondary, and tertiary packaging, cause the food in the packaging is potentially contaminated. One of the primary packaging materials is a duplex carton which is a product of recycled paper. Duplex carton is the most widely used primary material because of its cheaper and more eco-friendly. However, recycled paper is likely to contain printing inks, adhesives, waxes, fluorescent dyes and bleaches, fillers, organochlorine compounds, aromatic hydrocarbons, volatile organic compounds, barrier and oil-resistant materials, amines and surfactants. One of the hazardous substances contained in primary food packaging especially duplex carton is mineral oil or mineral oil hydrocarbon (MOH). Contamination of mineral oil into food packaging can be derived from printing ink contained on recycled paper or from printing ink on the packaging itself. Animal studies show that digestion of small amounts of mineral oil compounds can accumulate in the body and damage the liver and blood vessels in the heart, lymph nodes, and can be carcinogenic. This study aims to determine the potency of mineral oil migration from recycled paper-based food packaging. Migration test method that was used was the Federal Reference Laboratory for Food Contacts at the Federal Institute for Risk Assessment (BfR). Based on the analysis result both from internal standard adition at the beginning and at the end of the process showed that there is a potency of mineral oil to migrate from recycled paper based food packaging in this case is duplex carton.

Synthesis, Characterization and In vitro Cytotoxicity of Gold Nanoparticles Using Cultural Filtrate of Low Shear Modeled Microgravity and Normal Gravity Cultured K. pneumoniae

Duraisamy Kalpana,이양수,Krishnamoorthy Srikanth,PB Tirupathi Pichiah,차연수 한국고분자학회 2014 Macromolecular Research Vol.22 No.5

The cultural filtrates of Klebsiella pneumoniae cultured under normal gravity and low shear modeledmicrogravity were potentially used to biosynthesize gold nanoparticles using the precursor gold III chloride trihydrate. The gold nanoparticles produced were spherical, of size between 16-36 and 24-50 nm with the cultural filtratesof normal and low shear modeled microgravity respectively. The characteristic plasmon resonance of gold nanoparticlesoccurred between 535-550 nm in the spectral analysis. XRD reflections (1 1 1), (2 0 0), (2 2 0), (3 1 1) indicatethe face centered cubic and crystalline nature of the biosynthesized gold nanoparticles. The FTIR study proposesthat proteins excreted into the cultural filtrate were involved in the synthesis and stabilization of gold nanoparticles. The in vitro cytotoxicity on 3T3L1, H9c2 and HepG2 cell lines showed the nontoxic and biocompatible nature ofbiosynthesized gold nanoparticles.

시험관내 조직배양을 이용한 정상 및 비정상 자궁경부조직의 형태학적 연구

남궁성은(SE Namkoog),이충원(CW Lee),이상열(SY Lee),김필배(PB Kim),김세일(SI Kim),김승조(SJ Kim) 대한산부인과학회 1984 Obstetrics & Gynecology Science Vol.27 No.9

저자들은 정상 및 비정상 자궁경부조직의 시험관내 성장양상을 관찰하기 위하여 정상경부상피를 가진 6예의 정상여성, 3예의 상피내암 및 6예의 침윤성 경부암 환자로부터 얻은 총 36예의 자궁경부조직 절편을 가지고 미세수술 현미경을 이용한 시험관내 조직배양을 시행하여 자궁경부조직의 성장양상에 관한 다음과 같은 관찰결과를 얻었다. 1. 정상경부조직군에서는 세포형태가 균일한 형태로 성장하였으며 contact inhibition이 뚜렷하였으나 jumping현상은 볼 수 없었다. 비정상 경부조직군에서는 세포형태가 균일하지 못한 형태로 성장하였으며 contact inhibition이 없어지고 뚜렷한 jumping현상을 나타내었다. 2. 정상 및 비정상 자궁경부조직군에서는 배양시작 제 2주까지는 동일한 성장속도를 보여 주었으나 제 3주부터는 비정상 경부조직군에서만 현저한 성장을 보여 주었다. 3. 배양성적은 정상경부조직군보다 비정상 경부조직군에서 배양이 더 용이하였다. In order to observe the growth pattern of uterine cervical tissue in vitro, authors performed in vitro tissue culture using dissecting microscope with 36 samples of cervical tissue fragments obtained from 6 women with normal cervical epithelium, 3 patients with carcinoma in situ and 6 patients with invasive cervical carcinoma. The following results were obtained from the observation of the growth pattern of uterine cervical carcinoma. 1. In the normal cervical tissue group, the growth pattern was regular and contact inhibition was prominent, but jumping phenomenon was not found. In the abnormal cervical tissue group, the growth pattern was irregular and contact inhibition was disappeared. The prominent jumping phenomenon was found. 2. In the normal and abnormal uterine cervical tissue group, the growth pattern was same until 2nd week from the beginning of the culture. But from the 3rd week, the rapid growth pattern revealed in the abnormal uterine cervical tissue group. 3. Tissue culture was easier and more successful in the abnormal cervical tissue group than in the normal cervical tissue group.