Aspergillus sp.BH-31에 의한 Acid Amylase의 생산 및 효소학적 성질

곽병혁,양철영,이오택 한국산업식품제조학회 1998 산업식품제조학회지 Vol.2 No.1

토양시료에서 amylase를 강력하게 생산하는 균주를 분리 및 동정하여 최적 배양조건을 검토하였고 또한 효소를 정제하여 그 성질을 조사한 결과를 요약하면 다음과 같다. 균주는 형태학적, 배양학적 및 생리적 특성을 조사한 결과 Asprgillus sp.로 추정되었다. 배지조성을 soluble starch 5%, peptone 2%, Mg SO_(4)ㆍ 7H₂_(2)O 0.025%, KCl 0.01%로 하여 35℃, pH 3.0, 4일간 배양시 효소생산이 극대화되었다. 본 균주가 생산한 amylae를 정제하기 위한 과정으로 90% ethanol 침전, Sephadex G-100 gel filteration을 실시하였다. 효소의 최적 pH는 4.0이며, pH에 대한 안정성은 pH 4.0에서 가장 안정하였다. 최적 작용온도는 60℃에서 2시간 정도 열 안정성이 있었다. K^(+), Fe^(++)등의 이온에 의해서는 효소반웅이 촉진되었으며, Ag^(+).Cd^(++)등의 이온에 의해서는 효소활성이 억제되었다. 반응속도에 미치는 기질의 영향은 Lineweaver-Burk polt 상의 Km값은 4.0mg/㎖이었다. A saccharifying acid Amylase producing Aspergillius sp. strain BH-31 was isolated from soil, and conditions for the enzyme production, partial purification procedure and enzymatic properties were investigated. Acid-stable amylase production was maximized when the microorganism was grown in medium (initial pH 3.0) containing 5% soluble starch, 2% peptone, 0.025% MgSO_(4). 7H_(2)O, and 0.01% KCl for 4 days at 35℃. The enzyme was partially purified acid stable amylase were as follow: Optimal pH and temperature were 4.0 and 60℃. The enzyme was activated by K^(+), and Ba^(++) ion but inhibited by Ag^(+) and Cd^(++) ion. The Km value of amylase for soluble starch was 4.0mg/ml.

Evaluation of Vitamin $B_{6}$ Status and Korean RDA in Korean College Students Following a Uncontrolled Diet

Oho, Youn-Ok,Kim, Young-Nam The Korean Nutrition Society 2002 Nutritional Sciences Vol.5 No.1

The vitamin $B_{6}$ status of 49 healthy college student (women, aged 20-26 y) was estimated for evaluation of vitamin $B_{6}$ status and the Korean Recommended Dietary Allowance (RDA) for vitamin $B_{6}$. The average daily vitamin $B_{6}$ intake of the subjects was 0.86 $\pm$ 0.289 mg/d or 61.43 $\pm$ 24.10% of Korean RDA. The average ratio of vitamin $B_{6}$ intake to daily protein intake was 0.014 $\pm$ 0.003 mg/g protein. Foods from animal and plaint sources provided 34.25 $\pm$ 18.62% and 65.78 $\pm$ 18.72%, respectively, of total vitamin $B_{6}$. Plasma pyridoxal 5'-phosphate (PLP) concentration was significantly (p<.01 - p<.001) positively correlated to intakes of all other nutrients except vitamin C. However, no significant correlation was found between plasma PLP and nutrient intake. Vitamin $B_{6}$ intake only tended to have a positive correlation with plasma PLP concentration. Plasma total cholesterol was correlated to plasma PLP concentration (p<.05). Plasma PLP had no correlation with levels of glucose, triglyceride, and albumin. These results confirm that the present Korea RDA for vitamin $B_{6}$ of 1.4mg/d based on 0.02 mg/g protein is adequate.

Re-expansion of the Lung and Pneumothorax induced by Radiation Therapy in Lung Cancer with Atelectasis-One Case Report : One Case Report

Oho, Yoon-Kyeong,Oh, Keum-Tak 朝鮮大學校 附設 醫學硏究所 1991 The Medical Journal of Chosun University Vol.16 No.1

진행된 원발성 폐암 환자에서 국소 병변으로 인하여 심한 증상을 호소할 때, 증상완화를 위하여 종종 방사선 치료를 시행하게 된다. 최근 보고에 따르면 종양에 의한 기도 폐쇄로 무기폐를 동반한 비소세포성 폐암 환자에서 적절한 방사선 치료로 60% 이상에서 무기폐가 재팽창되었다고한다. 저자들은 폐암으로 인해 우측폐의 완전허탈을 보였던 환자에서 심한 호흡곤란의 완화를 위해 응급 방사선 치료를 시행하여, 허탈되었던 폐가 재팽창되면서 소량의 기흉이 발생되고,특별한 처치없이 수일후 기흉이 완전히 재흡수된 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Radiation therapy is frequently used to lung cancer of any cell type when it has a localized problem. Atelectasis from tumor obstruction is a common problem in the management of lung cancer. Recent data indicate that more than 60% of patients with non-small cell lung cancer presenting with atelectasis can have this problem relieved by appropriate radiation therapy. We report a case in which radiation therapy was used for relieving complete bronchial obstruction, and the collapsed lung was re-expanded but a small pneumothorax was developed due to re-expansion and reabsorbed spontaneously.

군사전략(軍事戰略)에 미치는 사회심리적(社會心理的) 요소(要素)

황기오 ( Ki Oho Hwang ) 국방대학교 안보문제연구소 1959 국방연구 Vol.4 No.-

Evaluating the Feasibility of Ohmic Heating to Cook Instant Rice Cake: Analysis of heat transfer characteristics, energy efficacy and textural qualities

Chang Ho Oho,Hwi Un Son,Yeon-Ji Jo,Sung Hee Park 한국산업식품공학회 2017 학술대회 및 심포지엄 Vol.2017 No.11

Ohmic heating is one of advanced thermal processing techniques which utilize conversion of electrical energy into heat. In our study, a feasibility of ohmic heating was tested to cook instant rice cake to improve energy efficacy as an alternative heating methods of conventional electrical kettle. Ohmic heating was conducted using customized ohmic cell (7.5×4.5×9.5 cm) equipped with titanium electrodes. Instant rice cakes in soup were ohmically heated up to 100°C at different electric fields (9, 12, 15 & 18 V/cm) and temperature holding times (60, 80, 100 & 120 s). Thermocouple was placed into both soup and rice cake to evaluate the temperature profile and energy efficacy. Temperature, voltage and current across the sample were measured and recorded at every 3 s using data acquisition system (DAQ). Mathematical model was developed to calculate the internal energy generation rate (QR, W). Internal energy generation rate (QR, W) was integrated versus temperature come-up time (s) to compute the total internal energy dose (ET, J) using MATLBA software. For energy efficacy (Eff), it was calculated ratio of total internal energy dose (ET, J) to heat quantity (Qh, J). During ohmic heating, temperature come-up time was significantly reduced as a function of elevated electric field (P<0.05). For example, 9V/cm of electric field showed 6.2±0.4 min of temperature come-up time up to 100°C. Higher electric field at 18 V/cm reduced temperature come-up time to 1.9±0.1 min. The electric field of 15 V/cm showed the best energy efficacy as 0.78 which meant 78% of electrical energy was converted into thermal energy for heating. In the texture profile analysis, the most preferable harness was found as 6.191 N at 15 V/cm and 100 s holding time. Our study showed the potential of ohmic heating to cook instant rice cakes for home meal replacement (HMR) and outdoor foods.

무선 환경의 의료 인증 시스템

오근탁(GuanTack Oho),이윤배(YunBae Lee),이성태(SungTae Lee) 한국정보과학회 2004 한국정보과학회 학술발표논문집 Vol.31 No.2Ⅰ

일반 사용자가 현재 접할 수 있는 무선인터넷은 SMS를 활용한 문자 형식의 정보제공이 대부분이고 유선의 인터넷과 같이 멀티미디어 서비스 그리고 실시간 전송속도, 사용자 환경, 거기에 보안 서비tm까지 유선의 인터넷 서비스와는 버교가 되지 않게 그 이용환경이 열악한 상황이다. 오늘날 원격 환경의 진료시스템이 개발되고 있는데 , 이들 시스템은 미래의 원격진료 즉, 병원에 직접가지 않고 집에서 바로 혈압, 심박수 등을 검사 받을 수 있는 시스템 개발의 기본이 되고 있다. 그리고 정보통신의 발전으로 모바일 PC 즉, 개인 휴대용 단말기 (PDA:Personal Digital Assistants)가 의료 분야에서 PC를 대체하여 이동성, 편리성을 제공하는 전자 차트를 선보이고 있다. 그러나 PDA 는 작은 몸체로 이동성 및 편리성 등이 PC 보다 뛰어나지만 , 해상도가 큰 이미지, 높은 처리 속도를 요구하는 작업 등을 처리하기에는 효율성이 낮은 문제점이 있다. 또한 정보를 공유할 수 있는 데이터를 무선 환경으로 처리 해야 하기 때문에 환자와 관련된 의료 영상 즉, MRI 사진이나 X-ray 사진 등을 의료 환경에 이용 하는 데는 보안 의 문제점을 가지고 있다. 따라서 본 논문에서는 매우 빠르게 발전하고 있는 진단과 치료기술을 이러한 의료를 필요로 하는 사람들에게 제공하는 접근성의 보장 문제를 해결할 수 있는 대안으로 무선 환경의 의료인증시스템을 제안 하고자 한다.

박하 器內培養에서 비타민과 inositol이 精油生産과 構成成分에 미치는 效果

Byung in Oho(吳炳仁),Young am Chae(蔡永岩) 한국육종학회 1991 한국육종학회지 Vol.23 No.4

This study was carried out to investigate effects of inositol and vitamines on the production of essential oil and its composition in order to know information for raising production of essential oil in Mentha piperita callus cultures. The results indicated that addtional concentration of myo-inositol increased the production of essential oil in Lin-Staba while it was decreased in MS-modify media. The menthol production was decreased in two media, although cell growth was increased in both media. When vitamin was added in twice as much concentration as basic media, the production of essential oil was increased in LinStaba while decreased in MS-modify media. The menthol production was decreased in two media increased with vitamin concentration.

영국의 신자유주의와 자유당의 사회개혁입법 ( 1908 - 1914 )

오인영(In Young Oho) 영국사학회 2001 영국연구 Vol.5 No.-

N/A

서평 : 박우룡, 《전환시대의 자유주의: 영국의 신자유주의와 지식인의 사회개혁》(신서원, 2003), Pp. 368

오인영 ( In Young Oho ) 영국사학회 2003 영국연구 Vol.10 No.-

결합 구형 루프 공진기를 이용한 IMT - 2000 단말기용 RF필터에 관한 연구

오태성(T. S. Oho),이영훈(Y. H. Lee),권원현(W. H. Kwon) 한국통신학회 2000 한국통신학회 학술대회논문집 Vol.2000 No.7