http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Boung-Jun Oh,Moon-Kyung Ko,Kwnag Sang Kim,김영순,Hyun Hwa Lee,전웅배,Kyung Hoan Im 한국분자세포생물학회 2003 Molecules and cells Vol.15 No.3
Unripe mature green fruits of pepper (Capsicum annuum) are susceptible to Colletotrichum gloeosporioides, whereas ripe red fruits are not. We established this pepper-C. gloeosporioides interaction as a model system to study the fungal resistance that develops during ripening of nonclimacteric fruit. Histochemical examination of transverse sections suggested that fungal invasion 24 h after inoculation (HAI) and colonization 48 HAI are critical events that differentiate between resistant and susceptible interactions. Based on this observation, we used messenger RNA differential display to isolate defense-related genes differentially expressed at 24 and 48 HAI. RNA gel blot analysis showed that six out of eighty cloned cDNAs were differentially expressed after infection of ripe fruit. The proteins encoded by these six clones, ddP1, ddP3, ddP4, ddP6, ddP13, and ddP47, showed significant homology to aldehyde dehydrogenase, P23 protein, NP24 protein, cytochrome P450 protein, esterase, and MADS-box protein, respectively, and may be involved in the resistance of ripe fruit to C. gloeosporioides infection
Evaluation of Genetic Diversity among the Genus Viola by RAPD Markers
Oh, Boung-Jun,Ko, Moon-Kyung,Lee, Cheol-Hee The Plant Resources Society of Korea 2006 한국자원식물학회지 Vol.19 No.6
The genetic diversity among the genus Viola was evaluated using the random amplified polymorphic DNA (RAPD) method. A total of 142 distinct amplification fragments by 18 random primers were scored to perform the cluster analysis with UPGMA. Viola species from the subsection Patellares were clustered into group I to IV. The groups from I to IV were consistent with its morphological taxonomy, series Pinnatae, Chinensis, Variegatae, and Patellares in the subsection Patellares, respectively. Even though V. albida and V. albida var. takahasii were classified in Chinensis, they were assigned into group I. The cluster analysis separated other subsections from Patellares in the section Nomimium. Interestingly, V. verecunda and V. grypoceras in subsections Biobatae and Trigonocarpae, respectively, were clustered into group C with a high similarity coefficient. Therefore, RAPD analysis can be used for providing an alternative classification system to identify genotypes and morphological characters of Viola species.
Oh Boung-Jun 한국자원식물학회 2003 한국자원식물학회 학술심포지엄 Vol.2003 No.-
Clonal propagation of high-value forest trees through somatic embryogenesis (SE) has the potential to rapidly capture the benefits of breeding or genetic engineering programs and to improve raw material uniformity and quality. A major barrier to the commercialization of this technology is the low quality of the resulting embryos. Several factors limit commercialization of SE for Corsican pine, including low initiation rates, low culture survival, culture decline causing low or no embryo production, and inability of somatic embryos to fully mature, resulting in low germination and reduced vigour of somatic seedlings. The objective was to develop a Corsican pine maturation medium that would produce cotyledonary embryos capable of germination. Treatments were arranged in a completely randomized design. Data were analyzed by analysis of variance, and significant differences between treatments determined by multiple range test at P=0.05. Corsican pine (Pinus nigra var. maritima) cultures were initiated on modified !P6 medium. Modifications of the same media were used for culture multiplication and maintenance. Embryogenic cultures were maintained on the same medium semi solidified with 2.5 g/l Gelrite. A maturation medium, capable of promoting the development of Corsican pine somatic embryos that can germinate, is a combination of iP6 modified salts, 2% maltose, 13% polyethylene glycol (PEG), 5 mg!l abscisic acid (ABA), and 2.5 g/l Gelrite. After initiation and once enough tissue developed they were grown in liquid medium. Embryogenic cell suspensions were established by adding 0.951.05 g of 10- to 14-day-old semisolid-grown embryogenic tissue to 9 ml of liquid maintenance media in a 250ml Erlenmeyer flask. Cultures were then incubated in the dark at 2022<TEX>$^{\circ}$</TEX>C and rotated at 120 rpm. After 2.53 months on maturation medium, somatic embryos were selected that exhibited normal embryo shape. Ten embryos were placed horizontally on 20 ml of eithe
Isolation and characterization of Bacillus amyloliquefaciens from Allomyrina dichotoma larva feces
Hyo-Song Nam,Sun-Am Kim,Boung-Jun Oh,Young-Cheol Kim 한국응용곤충학회 2011 한국응용곤충학회 학술대회논문집 Vol.2011 No.10
A bacterium was isolated from feces of Allomyrina dichotoma larva that feeds on well-rotted sawdust of woods. The isolate was identified as a Gram-positive and spore-forming stain. We named the isolate as Bacillus amyloliquefaciens on the basis of morphological and biochemical properties as well as 16S rRNA gene sequences. The culture of bacteria beneficially increased root and shoot growth of tomato, pepper and cucumber plants compared to the distilled water control. In addition, the bacterial culture strongly inhibited the mycelial growth of several fungal phytopathogens. The drop collapse assay with this culture showed a surfactant activity that is a major indicator for the selection of biocontrol agent. Also, a bacterium has ability of wastewater treatment. These data demonstrate the potential application of B. amyloliquefaciens as a biocontrol agent.
제비꽃속 제비꽃아절의 유전적 다양성과 RAPD 분석에 의한 계열별 marker의 개발
진연희,오병준,이철희,고문경 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 1996 연구보고서 Vol.1 No.-
제비꽃아절에 속하는 종들의 유전적 근연관계를 구명하기 위하여 30 종류의 종들과 그들의 교잡종을 사용하여 RAPD 분석을 실시하였다. 선발된 13개의 primers는 56개의 informative marker를 증폭하였으며, 이를 바탕으로 UPGMA 분석을 하여 Group A와 B로 집괴하였다. 제비꽃계열에 속하는 대부분의 종들(19종)과 남산제비꽃계열에 속한 남산제비꽃이 A그룹에 속했으며, A그룹은 A-Ⅰ, A-Ⅱ, A-Ⅲ의 3개의 아그룹으로 구분되었다. 이 dendrogram을 분석한 결과 각각의 아그룹들은 어느 정도 형태적인 특성과 일치하였다. 그러나 형태적 분류에 의해서는 다른 계열에 속하는 태백제비꽃, 단풍제비꽃과 남산제비꽃은 높은 유사도지수를 보이며 아그룹 A-Ⅲ에 함께 집괴되었다. 알록제비꽃계열에 속하는 7종류의 식물들과 뫼제비꽃계열에 속하는 자주잎제비꽃은 B그룹으로 집괴되었다. 화업제비꽃과 자주잎제비꽃의 양친을 구명하기 위하여 선발된 10개의 random primers로 RAPD 분석을 하여 44개의 informative bands를 얻었다. 이를 바탕으로 한 UPGMA 분석의결과 화업제비꽃은 자주잎제비꽃의 한쪽친으로 생각되는 남산제비꽃과 상대적으로 높은 유사도지수를 보였다. 또한 제비꽃과 호제비꽃 중의 하나가 화업제비꽃이나 자주잎제비꽃의 한쪽친인 것으로 생각된다. RAPD 분석에 의한 제비꽃속 식물의 유전적 분류는 전통적인 방법인 형태적 특성에 의한 분류와 대체적으로 일치하는 경향이었다 The genetic relationships of Viola in the Subsection Patellares were analyze using random amplified polymorphic DNA (RAPD) method. A total of 56 PCR products amplified by 13 random primers were used to perform for the cluster analysis with UPGMA. Nineteen Viola from the series Chinensis and V. chaerophylloides from the series Pinnatae were clustered into group A, and were subdivided into subgroups, A-Ⅰ, A-Ⅱ, and A-Ⅲ. Each subgroups to some extent corresponding to morphological characteristics were readily distinguished on this dendrogram. However, V. albida, V. albida var. takahasii, and V. chaerophylloides were clustered into A-Ⅲ with a high similarity coefficient, although they are classified into different series by morphological characteristis. Seven Viola species in he series Variegatae and V. violacea in the series Patellares were clustered into group B. In order to deduce putative parents of V. ibukiana and V. vioacea, ten primers selected from the previous study amplified 44 informative polymorphic products. From the cluster analysis, V. ibukiana had a relative high similarity coefficienct with V. chaerophylloides which might be one of putative parents of V. violacea. One of V. mandshurica and V. yedoensis may serve as another putative parents of V. ibukiana and V. violacea. Genetic relationships of Viola derived using data from RAPD analysis might be in agreement with conventional taxonomy from morphological characteristics