Antioxidant potential of buffalo and cow milk Cheddar cheeses to tackle human colon adenocarcinoma (Caco-2) cells

Nuzhat Huma,Saima Rafiq,Aysha Sameen,Imran Pasha,Muhammad Issa Khan 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.2

Objective: The aim of present study was to assess the anti-oxidant potential of water-soluble peptides (WSPs) extract derived from buffalo and cow milk Cheddar cheeses at different stages of ripening. Methods: The antioxidant potential of WSPs extract was assessed through 2,2'-azinobis-3-ethylbenzothiazoline-6sulfonic acid (ABTS)-radical scavenging activity. In addition, impact of WSPs extract on cell viability and production of reactive oxygen species (ROS) in human colon adenocarcinoma Caco-2 (tert-butylhydroperoxide-induced) cell lines was also evaluated. Results: The ABTS-radical scavenging activity increased progressively with ripening period and dose-dependently in both cheeses. However, peptide extract from buffalo milk Cheddar cheese demonstrated relatively higher activity due to higher contents of water-soluble nitrogen. Intracellular ROS production in Caco-2 cells decreased significantly (p<0.05) till 150th day of cheese ripening and remained constant thereafter. Additionally, dose-dependent response of WSPs extract on antioxidant activity was noticed in the Caco-2 cell line. Conclusion: On the basis of current in vitro study, the Cheddar cheese WSPs extract can protect intestinal epithelium against oxidative stress due to their antioxidant activity.

Clinical Efcacy and Safety of Injectable Levetiracetam Versus Phenytoin as Second-Line Terapy in the Management of Generalized Convulsive Status Epilepticus in Children: An Open-Label Randomized Controlled Trial

Nuzhat Noureen,Saadia Khan,Asim Khursheed,Imran Iqbal,Moallah Maryam,Syed Muhammad Sharib,Neeta Maheshwary 대한신경과학회 2019 Journal of Clinical Neurology Vol.15 No.4

Background and Purpose Tere is sparsity of quality evidence for the use of drugs afer frst-line benzodiazepines in convulsive status epilepticus in children. Te aim of the study was to compare the clinical efcacy and safety of intravenous levetiracetam versus intravenous phenytoin as second-line drugs in the management of generalized convulsive status epilepticus in children. Methods Tis open-label randomized controlled trial was conducted in the Emergency Department of Te Children’s Hospital and Te Institute of Child Health, Multan, Pakistan over a period of 4 years and 6 months from January 2014 to June 2018. Tis study included 600 children with generalized convulsive status epilepticus: 300 in the 40 mg/kg levetiracetam group, and 300 in the 20 mg/kg phenytoin group. Cessation of a clinical seizure (seizure cessation rate) within 30 minutes afer the end of drug administration was the primary outcome in this study, and the presence or absence of adverse efects was noted as the secondary outcome. Data were analyzed using SPSS (version 20.0). Results Te children in the levetiracetam and phenytoin were aged 3.5±0.2 and 3.4±0.2 years (mean±SD), respectively, their seizure durations before the start of treatment were 25.1±0.6 and 23.8±0.4 minutes, and their treatment efficacies were 278/300 (92.7%) and 259/300 (83.3%). Levetiracetam was signifcantly more efective than phenytoin (p=0.012), with no signifcant diference in safety. Adverse events were observed in eight children in the phenytoin group. Conclusions Levetiracetam is signifcantly more efective than phenytoin for the treatment of convulsive status epilepticus in children who have failed to respond to benzodiazepines.

The Development of an ADDIE Based Instructional Model for ELT in Early Childhood Education

( Nuzhat Mariam ),( Chang-woo Nam ) 한국교육공학회 2019 Educational Technology International Vol.20 No.1

The core purpose of the study is to develop and validate an ADDIE model based instructional model for English Language Teaching (ELT) in early childhood classroom in Bangladesh as an aid to teachers to reconstruct their knowledge and experience more strategically, and for them to design and implement their instruction more structurally. This study is developmental in nature which has been divided in five phases as follows. Phase I: Existing methods and instructional strategy review, Phase II: Instructional model development, Phase III: Delphi 1st round, Phase IV: Delphi 2nd round and Phase V: Model validation. After reviewing relevant literature and existing strategy in phase I, the 1st version of instructional model is made phase II. Next in phase III and phase IV, two rounds of Delphi have been conducted where experts related to different concerning areas of this study reviewed the 1st version and gradually the final version of the instructional model is made. Finally, the instructional model for English teachers of early childhood classroom in Bangladesh got validated by the same Delphi panelists in Phase V. In respect with each phases of ADDIE, the instructional model elaborates the 1) representative key points, 2) instructors’ activities prescribed for the instructors, 3) supporting strategies. Both the conceptual and procedural models are included in this study for clearer identification of the whole process. Lastly the study provides some recommendations for instructors and practitioners on choosing the instructional model like doing prior need analysis, incorporating teacher training programs, training students, keeping on researching for finding effective teaching technique and tools and being open to changes etc. In addition, the study also acknowledges its limitations like not being able to consider the psychological factors due to time limitation. Finally, at the end the study points out the areas that welcome further research.

"Somehow, I will convince my people, ... . I will have to follow but I won’t accept everything”: Asian women’s empowerment via higher education

Sara Nuzhat Amin 이화여자대학교 아시아여성학센터 2018 Asian Journal of Women's Studies(AJWS) Vol.24 No.2

This paper presents findings from a three-year longitudinal qualitative case-study exploring the experiences of Asian women returning home after completing higher education. Drawing on the literature on empowerment, education and identity, analyses point to the empowering and displacing aspects of education: While education enhances choice, awareness, self-confidence, and status in the family, it also produces differences which are utilized to delegitimize and destabilise the empowerment process, through frames of culture, class and gender. The paper addresses how these women negotiate these conflicting experiences, mitigate the alienation that empowerment and education processes can trigger, and maintain their new identities and aspirations. This contributes to understanding the complexity and multidimensional nature of empowerment dynamics for women in cross-cultural higher education processes in the Global South.

Early childhood teacher certification in major countries: criteria and curriculum

마리암 누즈하트(Mariam Nuzhat ) 학습자중심교과교육학회 2022 학습자중심교과교육연구 Vol.22 No.11

목적 본 연구의 주요 목적은 미국, 영국, 캐나다 및 호주의 유아교사 자격 기준을 살펴보고, 대학의 유아교사 교육과정을 비교⋅분석하여 한국의 유아교사 교육과정에 대한 의미 있는 시사점을 제공하는 것이다. 방법 이를 위하여 미국, 영국, 캐나다, 호주, 한국의 교육 관련 부서 홈페이지에 탑재되어 있는 유아교사 자격 관련 법령 및 기준에 대한 문서들을 검색하여 수집하였다. 또한 각 대학 홈페이지에 탑재되어 있는 유아교사 교육과정을 수집하여 비교, 분석하였다. 결과 이들 국가에서 유아 교사의 자격에 대한 국가 기준은 뚜렷한 것으로 밝혀다. 선택한 대학의 커리큘럼은 기간, 학위 유형 및 과정면에서 서로 다르다. 결론 본 연구에서 나타난 결과를 토대로 유아교사 교육과정의 교과목 구성, 실습기간 및 방법, 장애아동 및 다문화가정 아동 등 다양한 배경을 가진 아동에 대한 이해, 코로나19로 인한 하이브리드교육 등을 중심으로 한국 유아교사 교육과정 개선방향을 제안하고 논의하였다. Objectives The major purpose for this study is to explore the national criteria for early childhood teachers’ eligibility in USA, England, Canada, and Australia along with comparing and analyzing the existing curriculum of Columbia University, Harvard University, University of Illinois at Chicago, Cambridge University, University of Toronto, Australian college of teachers’ aides and children. Methods The study thoroughly analyzed the curriculum based on core course, method course and elective courses, duration of teaching practicum, type of course, way of course conduction: online or offline, inclusion of courses on special child and option of bilingual extension. Results National criteria for early childhood teachers’ eligibility in these countries are found to be distinctive. The curriculums of the selected universities also differ from one another in terms of duration, degree type and courses. Conclusions The study provides implications for early childhood educators training in Korea providing recommendation for instructors and practitioners on choosing courses for generating a well fitted curriculum for better teachers training.

Do Human Papilloma Viruses Play Any Role in Oral Squamous Cell Carcinoma in North Indians?

Singh, Vineeta,Husain, Nuzhat,Akhtar, Naseem,Kumar, Vijay,Tewari, Shikha,Mishra, Sridhar,Misra, Sanjeev,Khan, M.Y. Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.16

Background: Oral squamous cell carcinoma (OSCC) is the most prevalent malignancy among males in India. While tobacco and alcohol are main aetiological factors, human papilloma virus (HPV) presence has surprisingly increased in head and neck Squamous Cell Carcinoma (HNSCC) in the past two decade but its frequency in OSCCS is still uncertain. We aim to explore the frequency of HPV and its major genotypes in North Indian patients and their association with clinicopathological and histopathological features and p16 expression pattern. Materials and Methods: The study group comprised 250 histologically proven cases of OSCC. HPV was detected by real time PCR in tumor biopsy specimens and confirmed by conventional PCR with PGMY09/PGMY11 primers. Genotyping for high-risk types 16/18 was conducted by type specific PCR. p16 expression was assessed by immunohistochemsitry. Results: HPV presence was confirmed in 23/250 (9.2%) OSCC cases, of which 30.4% had HPV 16 infection, 17.4%were positive for HPV 18 and 26.1% had co-infections. HPV presence was significantly associated with male gender (p=0.02) and habit of pan masala chewing (p=0.01). HPV positive cases also had a history of tobacco consumption in 91.3% cases. p16 over expression was observed in 39.1% of HPV positive cases but this was not significantly different from negative cases (p=0.54). Conclusions: The frequency of HPV in OSCC is low in North-India and majority of cases are associated with a tobacco habit. It appears that tobacco shows a confounding effect in HPV positive cases and use of p16 protein as a reliable marker to assess the potential etiological role of HPV in OSCC in our population is not suggested.

Chemical Composition, Nitrogen Fractions and Amino Acids Profile of Milk from Different Animal Species

Rafiq, Saima,Huma, Nuzhat,Pasha, Imran,Sameen, Aysha,Mukhtar, Omer,Khan, Muhammad Issa Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.7

Milk composition is an imperative aspect which influences the quality of dairy products. The objective of study was to compare the chemical composition, nitrogen fractions and amino acids profile of milk from buffalo, cow, sheep, goat, and camel. Sheep milk was found to be highest in fat ($6.82%{\pm}0.04%$), solid-not-fat ($11.24%{\pm}0.02%$), total solids ($18.05%{\pm}0.05%$), protein ($5.15%{\pm}0.06%$) and casein ($3.87%{\pm}0.04%$) contents followed by buffalo milk. Maximum whey proteins were observed in camel milk ($0.80%{\pm}0.03%$), buffalo ($0.68%{\pm}0.02%$) and sheep ($0.66%{\pm}0.02%$) milk. The non-protein-nitrogen contents varied from 0.33% to 0.62% among different milk species. The highest r-values were recorded for correlations between crude protein and casein in buffalo (r = 0.82), cow (r = 0.88), sheep (r = 0.86) and goat milk (r = 0.98). The caseins and whey proteins were also positively correlated with true proteins in all milk species. A favorable balance of branched-chain amino acids; leucine, isoleucine, and valine were found both in casein and whey proteins. Leucine content was highest in cow ($108{\pm}2.3mg/g$), camel ($96{\pm}2.2mg/g$) and buffalo ($90{\pm}2.4mg/g$) milk caseins. Maximum concentrations of isoleucine, phenylalanine, and histidine were noticed in goat milk caseins. Glutamic acid and proline were dominant among non-essential amino acids. Conclusively, current exploration is important for milk processors to design nutritious and consistent quality end products.

A review on nutritional composition, health benefits, and technological interventions for improving consumer acceptability of camel meat: an ethnic food of Middle East

Waqas N. Baba,Nuzhat Rasool,M. Selvamuthukumara,Sajid Maqsood 한국식품연구원 2021 Journal of Ethnic Foods Vol.8 No.-

Camel meat is an ethnic food consumed across the arid regions of Middle East and North-East Africa. It can be a potential alternative red meat for human consumption worldwide. Camel meat is nutritionally as good as any conventional meat source, in fact has an edge over beef or lamb due to its low intramuscular fat, low cholesterol content, and high iron content. Camel meat quality is a function of age, breed, and type of muscle consumed. Various techniques such as aging, low-temperature storage, and pre-treatment with antioxidants improve the quality and shelf life of camel meat. Active packaging and fermentation are promising techniques to improve consumer acceptance and shelf-life of camel meat. Very limited research is available about the use of novel pre-treatments, packaging, and processing techniques that can improve the consumer acceptability of camel meat. Due to restricted use of camel meat and its products to ethnic regions, a review highlighting the nutritional potential and strategies to improve the quality of camel meat and its products may enhance its global acceptance as an alternative source of red meat.

Bioinformatics and Systems Biology : Identification and comperative genomics of the rat protein kinases

( Julhash U. Kazi ),( Nuzhat N. Kabir ),( Jae Won Soh ) 한국생화학분자생물학회 (구 한국생화학회) 2008 생화학분자생물학회 춘계학술발표논문집 Vol.2008 No.-

Comparison of Cervical Cell Morphology Using Two Different Cytology Techniques for Early Detection of Pre-Cancerous Lesions

Moosa, Najla Yussuf,Khattak, Nuzhat,Alam, Muhammad Irfan,Sher, Alam,Shah, Walayat,Mobashar, Shumaila,Alam, Muhammad Imran,Javid, Asima Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.2

Cervical cancer is an issue of foremost importance globally, specifically affecting the developing nations. Significant advances have taken place with regard to diagnosis of cervical cancer, especially with screening. Appropriate screening measures can thus reduce the incidence of cervical cancer. The most desirable screening technique should be less invasive, easy to perform, cost-effective and cover a wide range of diagnostic icons. Manual liquid based cytology (MLBC) can be considered as one of the suitable technique for screening with the above-mentioned benefits. The aim of the current study was to compare two cervical screening techniques on the basis of different morphological parameters and staining parameters by using modified acetic acid Pap staining to see the possibility of reducing time economy involved in conventional Pap staining (CPS). The study was conducted on a total 88 cases and all were analyzed with both MLBC and CPS. Forty eight cases that were regarded as satisfactory on the basis of Bethesda system by both methods were further recruited for investigation. Their morphological parameters and staining quality were compared and scored according to a scoring system defined in the study. Quality indices was calculated for both staining procedures and smear techniques.