RISS 검색 - 국내학술지논문

무료
기관 내 무료
유료

내보내기
내책장담기
한글로보기

정확도순

내림차순

내림차순

10개씩 출력

1
Oral glucose tolerance test for preoperative assessment of liver function in liver resection

Saravanan Manickam Neethirajan,Raghavendra Rao Rachapoodivenk 한국간담췌외과학회 2017 Annals of hepato-biliary-pancreatic surgery Vol.21 No.1
- 원문보기
- 복사/대출신청
Backgrounds/Aims: We intended to determine the role of the Oral glucose tolerance test (OGTT), in addition to volumetry, in preoperative assessment of patients undergoing liver resection. Methods: This was a prospective study conducted at a tertiary care hospital, between February 2009 and February 2011. OGTT curve (parabolic/linear), linearity index (LI) and Parenchymal Hepatic Resection Rate (PHRR) were correlated with postoperative outcomes in terms of postoperative liver failure (PLF), by 50-50 criteria, morbidity, mortality and hospital stay. Results: Of the 33 patients included in the study, 23 (69.7%) patients underwent major liver resections. Hepatocellular carcinoma (30.3%) was the leading indication. The overall postoperative morbidity rate was 72.7%, but major complications occurred in 3 (9.1%) patients only. There was no 90-day mortality. The 50-50 criteria were met by 3 patients undergoing major resection. Significant correlation was noted between the linear OGTT curve and the overall hospital stay (12.1 days vs. 9.6 days in parabolic; p=0.04). Patients with linear OGTT met the 50-50 criteria more often (18%) than those having a parabolic curve (4.5%; p=0.25). Although the OGTT was more often linear with occurrence of morbidity (41.7% vs 11.1%), major morbidity (66.7% vs 30%) and PLF by 50-50 criteria (66.7% vs 30%), it was not statistically significant. The linearity index was marginally lower (0.9 vs 1.2) in the presence of major morbidity and PLF by 50-50 criteria. Conclusions: Linear OGTT affects the PLF and major morbidity, therein impacting the hospital stay. OGTT LI and PHRR can help predict postoperative outcome for a given extent of liver resection.
2
An in vitro Microfluidic Gradient Generator Platform for Antimicrobial Testing

Matthew DiCicco,Suresh Neethirajan 한국바이오칩학회 2014 BioChip Journal Vol.8 No.4
Methicillin Resistant Staphylococcus pseu-dintermedius (MRSP) biofilm-related infections arecurrently a leading concern for veterinary hospitals, asthese types of infections are highly resistant to assaultsby both the immune system and antimicrobial thera-pies, impeding their clearance. Research suggests thatfosfomycin, a low molecular weight bactericidal anti-biotic, has the potential to effectively penetrate andsubsequently disrupt/destroy biofilm layers. Our studyutilized a fabricated microfluidic gradient generatorplatform as an assay to perform a quantitative assess-ment of varying concentrations of a selected antimi-crobial agent against MRSP biofilm formed under phy-siologically relevant conditions. Our results verifiedthe feasibility of using a microfluidic device for rapidantimicrobial testing against biofilms, which was suc-cessful in demonstrating that fosfomycin is an effec-tive agent that can disrupt established MRSP biofilms. Additionally, Atomic Force Microscopy (AFM) analy-sis revealed that the cell walls of MRSP cells withinthe biofilms were disrupted by fosfomycin treatment,which speaks to the mechanism of action and the anti-microbial efficacy of this agent. This study providescompelling evident that microfluidic device and nano-scale AFM imaging-based investigations of biofilmscan aid in the study of biofilm-related infectious dis-eases.
3
Detection of protein kinase using an aptamer on a microchip integrated electrolyte-insulator-semiconductor sensor

Chand, Rohit,Han, Dawoon,Neethirajan, Suresh,Kim, Yong-Sang Elsevier 2017 Sensors and actuators. B Chemical Vol.248 No.-
- 원문보기
Abstract Herein, we developed a microchip electrolyte-insulator-semiconductor (EIS) sensor for the capacitive detection of protein kinase A (PKA). EIS sensing is customarily performed in a Teflon cell to define the sensing area. However, in this work, a rapid prototyping technique was followed to integrate polymeric microchip, a reference electrode, and the EIS sensor. The aptameric peptide was used for one-step and label-free detection of PKA enzyme. The thiolated PKA-specific aptamer was immobilized on the gold nanoparticles decorated EIS sensor surface. The detection of PKA in microchip was based on the change in surface charge of EIS sensor. We also analyzed the ability of microchip-EIS sensor to distinguish between buffers at different pH. An average sensitivity of 96mV/pH for a pH range of 5–9 was obtained. The quantitative detection of PKA was performed by analyzing the capacitance-voltage curve after the aptamer-PKA interaction. The EIS sensor showed a detection limit of 2U/mL with a relative linearity from 10U/mL to 80U/mL for the detection of PKA. This study proposes an integrated and point-of-care applicable biosensor for the rapid diagnosis. Highlights <UL> <LI> The EIS sensor integrated with microchip and reference electrode was fabricated. </LI> <LI> The microchip-EIS was used for detection of protein kinase. </LI> <LI> The change in surface charge alters the VG of EIS sensor. </LI> <LI> PKA was efficiently detected on-chip using aptamer. </LI> <LI> The microchip-EIS was highly specific, selective and stable. </LI> </UL> Graphical abstract [DISPLAY OMISSION]