위발성 위장관 악성림프종 환자에서 항암치료의 효과

김찬규,신영록,김현정,배상병,이남수,이규택,박성규,원종호,홍대식,박희숙 순천향대학교 2006 Journal of Soonchunhyang Medical Science Vol.12 No.1

Purpose: The gastrointestinal (GI) tract is the most common site of extranodal non-Hodgkin's lymphoma (NHL), which is increasing in incidence, but there is no established optimal treatment modality. Thus, this study was investigated the clinicohistologic feature, the therapeutic modalities, and the prognosis for GI-NHL, as well as the factors affecting it. Methods: We retrospectively analyzed 45 patients who had been diagnosed as having GI-NHL and had been followed up from July 1994 to February 2005 at Soonchunhyang University Hospital. The patients were divided into groups according to the site of origin and to various other features, and the survivals of the various groups were compared. The modified Ann Arbor system and WHO classification were adopted for staging and histopathologic classification, respectively. Results: GI-NHL of the stomach, small bowel, ileocecal region, and colon occurred in 28 patients (62.2%), 5 patients (11.1%), 3 patients (6.7%), and 8 patients (17.8%), respectively, In one patient, the entire gastrointestinal tract was diffusely involved. The median age of patients was b5 years (25~78 years), and male-to-female ratio was 1:1.1. Fourteen patients were in stage Ⅰ, 24 in stage Ⅱ, 4 in stage Ⅲ, and 3 in stage Ⅳ. Surgical resection was performed in 19 patients, and combination chemotherapy was performed in 43 patients. Surgical resection only was performed in 4 patients, Chemotherapy only was performed in 26 patients. The expected overall 5 year survival of 45 patients was 39.6%, and there was a significant survival difference between the stages, but between sites of origin (p=0.842). The most important factors influencing the survival was the stage and other factors were not significant. Conclusion: The stomach was the most common site of GI-NHL. Most GI-NHL were localized Stage was the most important prognostic factor. However, Prospective randomized studies are needed to approve the therapeutic modality.

Effects of epigallocatechin gallate on CoCl_(2)-induced apoptosis in PC12 cells

Yang, Kyu-Ho,Mo, Hyun-Chul,Choi, Nam-Ki,Kim, Seon-Mi,Kim,Won-Jae 大韓小兒齒科學會 2006 大韓小兒齒科學會誌 Vol.33 No.1

Neuronal apoptotic events, consequently resulting in neuronal cell death, are occurred in hypoxic/ischemic condition. This cell death has been shown to be accompanied with the production of reactive oxygen species (ROS), which can attack cellular components such as nucleic acids, proteins and phospholipid. However, the underlying mechanisms of apoptosis induced in hypoxic/ischemic condition and its treatment methods are unsettled. Cobalt chloride (CoCl_(2)) has been known to mimic hypoxic condition including the production of ROS. Epigallocatechin gallate (EGCG). a green tea polyphenol, has diverse pharmacologial activities in cell growth and death. This study was aimed to investigate the apoptotic mechanism by CoCl_(2) and effects of EGCG on CoCl_(2)-induced apoptosis in PC12 cells. Administration of CoCl_(2) decreased cell survival in dose- and time-dependent manners and induced genomic DNA fragmentation. Treatment with 100 µM EGCG for 30 min before PC12 cells were exposed to 150 µM CoCl_(2), being resulted in the cell viability and DNA fragmentation being rescued. CoCl_(2) caused morphologic changes such as cell swelling and condensed nuclei, whereas EGCG attenuated morphologic changes by CoCl_(2). EGCG suppressed the apoptotic peak and a loss of Δψ_(m) induced by CoCl_(2). CoCl_(2) decreased Bcl-2 expression but Bax expression was not changed in CoCl_(2)-treated cells. EGCG attenuated the Bcl-2 underexpression by CoCl_(2). CoCl_(2) augumented the cytochrome c release from mitochondria into cytoplasm and increased caspase-8, -9 and caspase-3 activity, a marker of the apoptotic executing stage. EGCG ameliorated the incruement in caspase-8, -9 and -3 activity, and cytochrome c release by CoCl_(2). NAC (N-acetyl-cysteine), a scavenger of ROS, attenuated CoCl_(2)-induced apoptosis in consistent with those of EGCG. These results suggest, that CoCl_(2) induces apoptotic cell death through both mitochondria- and death receptor-dependent pathway and EGCG has neuroprotective effects against CoCl_(2)-induced apoptosis in PC12 cells. 신경세포자멸사는 저산소 및 허혈환경에서 일어나며 이러한 세포죽은 reactive oxidant species (ROS) 생성을 동반함이 알려져있다. 그러나, 저산소 및 허혈환경에서 일어나는 세포자멸사의 기전 및 그 치료방법은 아직 정립되어 있지 않다. CoCl_(2)는 ROS를 생성하는 등 저산소환경과 유사한 조건을 초래하는 것으로 알려져 있다. Epigallocatechin gallate (EGCG)는 녹차의 polyphenol 성분으로서 세포성장과 죽음에 다양한 약리학적 효과를 나타냄이 알려져 있다. 본 연구는 PC12 세포에서 CoCl_(2)에 의한 세포자멸사기전을 밝히고 이에 미치는 EGCG의 효과를 조사하는데 목적이 있다. Cell viability는 MTT 측정으로 조사되었고, DNA fragmentation은 DNA laddering으로 조사되었다. Bcl-2와 Bax발현 정도는 RT-PCR로, caspase-3와 -9의 활성은 spectrophotometer, caspase-8의 활성은 flow cytometry에 의해 측정되었다. 미토콘드리아에서 세포질로 분비된 cytochrome c는 western blot으로, 분해된 DNA 양과 미토콘드리아 세포막전위 (Δψ_(m))는 FACScan으로 조사되었다. CoCl_(2)투여로 PC12 세포수는 용량 및 시간 의존형태로 감소하였고, genomic DNA fragmentation이 발생하였다. CoCl_(2)투여로 야기된 cell viability의 감소와 DNA fragmentation은 EGCG 전처치에 의해 억제되었다. CoCl_(2)은 세포용적팽창과 condensed nuclei 같은 형태적 변화를 일으켰으며, apoptotic peak, Δψ_(m)감소 및 cytochrome c 유리를 야기하였다. EGCG는 CoCl_(2)에 의한 세포형태변화, apoptotic peak, Δψ_(m)소실 및 cytochrome c 유리를 억제시켰다. CoCl_(2)는 Bcl-2 발현을 감소시켰지만, Bax 발현에는 영향을 미치지 않았다. EGCG는 CoCl_(2)에 의해 야기된 Bcl-2 발현 감소를 억제시켰다. CoCl_(2)는 caspase-3, -8, 그리고 -9의 활성을 증가시켰으며, EGCG는 CoCl_(2)에 의한 세포자멸사를 억제시켰다. 본 실험결과는 PC12 세포에서 CoCl_(2)가 미토콘드리아 의존 및 death receptor의존 기전으로 세포자멸사를 일으키며, EGCG는 세포자멸사기전을 억세지킴으로 신경보호기능을 가짐을 시사하였다.

양식 넙치(Paralichthys olivaceus)에서 발생한 랩도바이러스성 질병의 병리조직학적 특징

이남실,강형길,최희정,전세규,박남규,허민도 한국어병학회 2002 한국어병학회지 Vol.15 No.1

2000년 4월경부터 (수온7-13℃) 우리나라 동해안과 남해안 육상 수조에서 사육되던 넙치에서 체색흑화, 복수저류, 비장종대의 육안적 소견을 보이는 질병이 발생하여 대량폐사를 불러왔다. 병리조직학적 소견으로, 비장과 신장의 조혈조직에서 괴사병변이 주로 나타났다. 비장과 신장의 간질조직에서 괴사소가 관찰되었으며, 임파구, 괴사세포의 침윤이 나타났다. 비장 조직의 전자현미경적 관찰에서 변성혈구 내 세포질에서 탄환형의 바이러스 입자가 관찰되었는데 직경과 길이가 각각 약 50nm, 170nm였다. 이상의 결과로 본 질병은 rhabdovirus에 의한 질병으로 판단되었다. On April of 2000 and February of 2001, a disease characterized by darkening, ascites and enlargement of the spleen occurred in flounder, Paralichthys olivaceus, farmed near northern and eastern coasts of Korea. Various degree of necrosis including liquefactive-type was observed mainly in hemopoietic tissue of spleen and kindney. Certain tissue such as branchial soft tissue including epithelium showed a similar necrotic findings. But some tissues such as intentine had several necrotic clusters of wandering cells, being possibly leukocytes. Necrotic evidences of blood cells were evident in various tissues including hemopoietic tissues. Necrobiotic bodies were frequently encountered in blood smear preparations. Bullet type-virious were detected in the spleenic cells. Each virion was about 500 nm in diameter and 170nm in length. From these results, this disease was thought to be a rhabdoviral disease.

현대시조의 형식론적 계승에 대한 비판적 검토

김남규(Kim, Nam-Kyu) 한국시학회 2015 한국시학연구 Vol.- No.44

본 논문은 현재까지 창작되고 있는 현대시조가 ‘과연 정형시인가’라는 질문과 함께, ‘음보(音譜)’라는 개념의 불분명함을 논의하면서 현대시조의 형식론적 계승을 검토하였다. 음절수가 아닌 음량(mora)으로 음보율을 설명하려는 의도에서 우리는 음량이라는 단위는 객관적 실체로 입증하지 못한 채 막연한 심리적 경향으로 제시될 수밖에 없으며, 보편적 언어 체계 안에서 변별적 자질로 인정되기 어렵다. ‘등장성(等張性)’이라는 개념과 발음에 따른 음성학적 휴지에 의한 음보라는 개념에는 이미 통사적으로나 음성학적으로나 심리적 혹은 무의식적 배려가 작동하고 있음을 알 수 있다. 또한 통사적 구분에 따라 나눠지는 경계와 설정된 음보는 정확하게 일치하지도 않으며, 시의 특성과 시인 개별 발화 그리고 독자 모두와 상관없는 추상적인 마디 설정을 그 한계로 떠맡는다. 시의 의미와 상관없는 통사 구분은 시의 호성(ambiguity)과 결정불가능성(undecidability)의 기회조차 박탈해버리고, 하나의 음보 단위 안에 해석의 여지를 가둬버린다. 이에 따라 종장의 제2음보가 초중장의 음보보다 ‘과(過)’한 구조라는 것만 규칙으로 확정지을 수 있다. 고시조에서는 강한 정서적 중량을 가진 어사가 종장 제1음보를 이뤘으나, 현재에 이르면서 종장 제1음보는 3음절이라는 조건만 남게 되었다. 탈형식의 방식으로 시조가 현재까지 변형되며 지속되어 왔기 때문이다. 따라서 새로운 시조의 음보론이 요청되며, 현재 논의되고 있는 일반 자유시에 대한 리듬 연구에서도 마찬가지로 음보에 대한 논의는 계속 새롭게 씌어져야 한다. This study examined formalistic succession of modern sijo, discussing the ambiguity of the concept of ‘metre(音譜)’ as well as questioning ‘Is modern sijo still being composed until now really a fixed verse?’ Because of the intention that foot meter is explained not by the number of syllables, but by mora, the unit of mora can not help being presented as a vague psychological tendency without any objective reality, and it is difficult to recognize the mora as a distinctive feature in universal linguistic system. It is verified that the concept of metre by phonological pause according to the concept of ‘isometricity(等張性)’ and pronunciation is already operated by psychological or unconscious consideration in terms of syntax or phonetics. In addition, boundary by syntactic division didn’t exactly match with set metre. The characteristics of the poem, the poet’s own individual articulation, and the reader all took the limitation of irrelevant abstract foot set-up. Regardless of the meaning of the poem, syntactic division deprived the chances of poetic ambiguity and undecidability and confined any other choice of interpretation inside of one single foot unit. Accordingly, it is possible to make a rule that the second foot of the last of the 3 verses sijo is ‘more(過)’ stretched structure than the other verses. Although Ancient Korean sijo had the first foot of the last verse with more emotional tone, there has remained only the rule that the first foot of the last verse has 3 syllables up to modern times. It is because the trend of avoiding the formalism has transformed and continued to be reflected on the modern sijo. Therefore, it is necessary to suggest that a new theory of sijo metre is required, and rhythm research with present discussion on the general free verse should be reviewed in terms of metre as well.

다발성 관동맥-좌심실간 미세루 1예

정성원,김남호,박은미,이상재,윤경호,유남진,이은미,박무림,오석규,정진원,박옥규 圓光大學校 醫科學硏究所 2003 圓光醫科學 Vol.18 No.1

Luria-Nebraska 神經心理檢査의 韓國版 標準化硏究(Ⅱ)

金在煥,南正鉉,金光日,金二泳,黃淳澤,김지혜,金南奎,朴康圭,林東洙,金圭泰 漢陽大學校 精神健康硏究 1990 精神健康硏究 Vol.9 No.-

재발 또는 불응성 비호즈킨 림프종 환자에서 CDME 구제항암화학요법 후 고용량 항암화학요법 및 자가말초혈액 조혈모세포이식의 효과

김세형,한강원,배상병,김찬규,이남수,이규택,박성규,원종호,홍대식,박희숙 순천향의학연구소 2004 Journal of Soonchunhyang Medical Science Vol.10 No.1

Background and objectives : The long-term survival in patients with non-Hodgkin's lymphoma (NHL) after conventional dose chemotherapy is about 35% and the rest of the patients tend to have relapse. So, in relapsed or refractory NHL, we compared the outcome of patients undergoing high-dose chemotherapy with autologous peripheral blood stem cell transplantation(APBSCT) with only salvage chemotherapy of cisplatin, dexamethasone, mitoxantrone, and etoposide(CDME). Materials and methods : From June 1993 to December 1999, 25 patients with relapsed or resistant NHL were treated with CDME regimen as salvage chemotherapy. Twelve patients were received four cycles of CDME chemotherapy, and 13 patients were received high-dose chemotherapy with APBSCT following two cycles of CDME chemotherapy. Results : The median follow-up duration was 12.8 months(range:4-68). The overall response rate was 41.7% (complete response rate 25%, partial response rate 16.7%) in 12 patients with CDME only. Thirteen patients who were treated with high-dose chemotherapy with APBSCT achieved 61.5% complete response rate and 15.4% partial response rate, with an overall response rate of 76.9%. The estimated 3-year progression-free survival rate was significantly higher among patients who received high-dose therapy than patients who received CDME only(41.5% vs 20.0%, p<0.05). And, 3-year overall survival rate was significantly higher among patients who received high-dose therapy(51.3% vs 25.0%, p <0.05). Conclusions : In relapsed or refractory NHL, CDME chemotherapy is an effective salvage chemotherapy and allow peripheral blood stem cell collection. Also, high-dose chemotherapy with APBSCT following CDME is superior to CDME salvage chemotherapy only.

흉선종 제거후에 발생한 재생 불량성 빈혈 : 증례보고

전원선,이상철,김현정,배상병,김찬규,이남수,박노진,이규택,박성규,홍대식,박희숙,원종호 순천향의학연구소 2007 Journal of Soonchunhyang Medical Science Vol.13 No.2

Thymoma is associated with myasthenia gravis, Pure red cell aplasia, and autoimmune diseases such as autoimmune hemolytic anemia, aplastic anemia, and hypogammaglobulinemia. It's association with aplastic anemia is rare and aplastic anemia appearing after surgical removal of thymoma is especially rare. The authors hereby report a case of aplastic anemia occuring in a patient who was diagnosed with thymoma and myasthenia gravis and had the tumor surgically removed. The patient was treated with anti-thymocyte globulin, cyclosporin, and prednisolone, and showed partial remission with hematologic improvements after 12 months.

가속수명실험을 이용한 케이블 절연재료의 수명특성에 관한 연구

남창우,이규철,박홍태,이태식,김경석 울산대학교 1997 공학연구논문집 Vol.28 No.1

본 연구는 고체절연재료의 수명예측의 일환으로서 가속수명실험을 통해 얻은 수명데이터의 통계학적 처리방법과 복합 스트레스와 수명과의 상호관계를 규명하기 위한 수명모델을 제시하고 있다. 가속수명실험을 통해 얻는 수명데이터는 와이블분포에 적용하고, 최소자승법과 최우추정법을 이용하여 와이블분포함수의 모수를 추정하였다. 또한 제시된 수명모델의 상수를 추정하여 복합스트레스와의 관계를 규명함으로써 수명모델의 타당성을 입증하였다. 추정결과, 주위온도와 인가전압이 상승함에 따라 수명은 현저히 짧아지고, 수명모델의 상수는 복합스트레스에 크게 의존하는 것으로 나타난다. 따라서 본 연구에서 제시된 수명모델은 고체절연재료의 수명을 나타내는데 적당한 것으로 나타났다. This paper suggests the statistic method for a lifetime estimation of solid insulating materials using the data obtained from accelerated aging test has been performed to obtain the lifetime data. The multi-stress including thermal and electrical stress has been applied to accelerate the degradation of insulating materials. The Weibull Cumulative Distribution Function has been used to treat statistically the lifetime data. Using the lifetime model of this study, we estimated the lifetime constants to show the relationship between the lifetime and the applied multi-stress. It has been shown that the lifetime model agrees well with the lifetime of solid insulating materials.

알레르기성 소인이 없는 정상인에서 특이 알레르겐항체 양성율

남해선,안현철,박준수,황규윤,박춘식 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

This study was designed to evaluate prevalence of allergen-specific antibodies in healthy adults. During Mar. ~ Aug. 2001, 1,925 subjects visited for medical check-up, were inquired to fill out a standardized questionnaire. Among them, 200 subjects who had no current and past allergic disorders were randomly selected for rhe current study and examined for allergen-specific antibodies using Korean inhalant panel of MAST-CLA allergen-specific IgE assay. Among them, 170 persons (85.0%) did not have any positive allergen-specific antibody. Each positive rate of 35 allergen specific antibodies was usually less than 3%, except Cockroach Mix (3.5%), Dermatophagoides farinae(8.5%), Dermatophagoides pteronyssinus(6.0%). Among 30 positive subjects, 19(63.3%) showed one or two allergen-specific antibodies. Although atopic subjects in non-allergic population were not common, few of them showed allergen-specific antibodies in Korean inhalant panel of MAST-CLA. Our results suggested that the standardized questionnaire and MAST-CLA could be an useful tool of a screening test for allergy, showing high specificity and sensitivity.