Time-evolving genetic networks reveal a NAC troika that negatively regulates leaf senescence in <i>Arabidopsis</i>

Kim, Hyo Jung,Park, Ji-Hwan,Kim, Jingil,Kim, Jung Ju,Hong, Sunghyun,Kim, Jeongsik,Kim, Jin Hee,Woo, Hye Ryun,Hyeon, Changbong,Lim, Pyung Ok,Nam, Hong Gil,Hwang, Daehee National Academy of Sciences 2018 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.115 No.21

<▼1><P><B>Significance</B></P><P>Leaf senescence is regulated in a complex manner, involving time-dependent interactions with developmental and environmental signals. Genetic screens have identified key regulators of senescence, particularly late-stage senescence regulators. Recently, time-course gene-expression and network analyses, mostly analyses of static networks, have predicted many senescence regulators. However, senescence is defined by time-evolving networks, involving the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks of NAM/ATAF/CUC (NAC) transcription factors, central regulators of leaf senescence in <I>Arabidopsis</I>, via time-course gene-expression analysis of NACs in their mutants. These time-evolving networks revealed a unique regulatory module of NACs that controls the timely induction of senescence-promoting processes at a presenescent stage of leaf aging.</P></▼1><▼2><P>Senescence is controlled by time-evolving networks that describe the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks for NAM/ATAF/CUC (NAC) transcription factors in <I>Arabidopsis</I> during leaf aging. The most evident characteristic of these time-dependent networks was a shift from positive to negative regulation among NACs at a presenescent stage. ANAC017, ANAC082, and ANAC090, referred to as a “NAC troika,” govern the positive-to-negative regulatory shift. Knockout of the NAC troika accelerated senescence and the induction of other <I>NAC</I>s, whereas overexpression of the NAC troika had the opposite effects. Transcriptome and molecular analyses revealed shared suppression of senescence-promoting processes by the NAC troika, including salicylic acid (SA) and reactive oxygen species (ROS) responses, but with predominant regulation of SA and ROS responses by ANAC090 and ANAC017, respectively. Our time-evolving networks provide a unique regulatory module of presenescent repressors that direct the timely induction of senescence-promoting processes at the presenescent stage of leaf aging.</P></▼2>

야생 등줄쥐 (Apodemus agrarius coreae) 간뇌의 Dopamine성 신경세포

정영길,이남섭,민성기,김무강 대한해부학회 1997 Anatomy & Cell Biology Vol.30 No.6

손상받은고양이 줄무늬체에서 축삭반응에 관한 미세구조적 연구

양남길,조영열,안의태,고정식,박경호,김진국 대한해부학회 1994 Anatomy & Cell Biology Vol.27 No.3

등줄쥐 연수의 Tyrosine Hydroxylase, Dopamine-β-Hydroxylase 및 Phenylethanolamine-N-Methyltransferase 면역반응신경세포

정영길,이남섭,민성기 대한해부학회 1998 Anatomy & Cell Biology Vol.31 No.3

전략적 제휴를 통한 통관업체의 경쟁력 강화 방안에 관한 실증연구

성남길, 이기웅 건국대학교 사회정책연구소 2009 사회과학연구 Vol.23 No.-

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식물플라크톤의 세포외배출유기물을 고려한 소양호의 1차생산과 유기물 부하

남궁현,김범철,황길순,최광순,김철구 江原大學校 附設 環境硏究所 2001 環境硏究 Vol.18 No.-

The autochthonous and allochthonous organic carbon loading were measured in Lake Soyang, to estimate the amount of carbon loading into the lake and the contribution of their sources to the lake's carbon loading. Autochthonous carbon loading was estimated from phytoplankton primary production with the extracellular organic carbon(EOC). Allochthonous loding was determined by measuring dissolved organic carbon(DOC) and particulate organic carbon(POC) concentration in the main inflowing Soyang River. Both autochthonous and allochthonous organic carbon loading were high during the summer, from July to September, and accounted for 43.2% and 71.7% of the annual loading, respectively. Primary productivity was elevated up to 1,000 mgCm^(-2)d^(-1)during summer and lowest in winter. EOC production from phytoplankton was also large in summer, resulting in a high DOC concentration in the lake water. Primary production of phytoplankton and allochthonous organic matter loading from the watershed contributed to 53.6% and 46.4% of total loading, respectively. The EOC production accounted for 4.4~21.2% of POC primary production, implying that EOC production of phytolankton must be considered in estimation of primary production.

pV47-2 다좌위탐식자를 이용한 인체 게놈에서 다형성 유전좌위의 분리

남용석,이혜린,한길로,황적준 大韓法醫學會 1997 대한법의학회지 Vol.21 No.2

Two polymorphic loci, so- called FS106 and FS185, have been isolated from the human genome, using a multilocus probe pV47-2, which is extensively used in Korea for forensic investigation such as resolving paternity disputes. Among the several plaques selected from λ Fix-II genomic libraries, fourteen clones have been characterized. Restriction maps of 14 clones were constructed to define the flanking as well as repeat parts. The repeat-free flanking DNA fragments were tested for single locus specific polymorphism, and repeat containing DNA fragments were sequenced for the design of PCR primers. None of the repeat-free flanking DNA fragments was not shown any polymorphisms by RFLP analysis. The (GGT)??-rich sequences in most of repeat containing DNA fragments were identified by sequencing analysis. Most of repetitive sequences consists of major units of (GGT)??, but a regular repetition pattern can not be found in all clones. Two sets of primers designed from flanking sequences of repeat containing DNA fragments were shown length polymorphisms by PCR analysis, when tested in 50 unrelated individuals. Three and four alleles were detected at FS106 and 0.58 for FS185. In addition, two loci, FS106 and FS185, have been mapped on chromosome 5 and 3, respectively, by somatic cell hybrid analysis.

洛東江 河口堰 建設 후 養殖 海藻類의 病害 原因에 관하여

金南吉 國立統營水産專門大學 附設 水産科學硏究所 1994 수산과학연구소보고 Vol.5 No.-

본 연구는 낙동강 하구둑 건설 이후의 해조 양식장의 병해 현상을 구명하기 위하여 1989년 9월부터 1990년 4월까지 수행하였다. 양식기간중 강수량, 일조량 기온에서 부산지방의 기상 특이성이 나타났다. 하구둑 건설 후 장림천의 영향을 직접 받는 수로 부근의 해역에서 COD와 영양염류의 농도는 증가한 반면 pH와 염분도는 감소하였다. 양식 김과 파래는 구멍갯병, 녹반병, 붉은갯병, 횐갯병. 쪼그랑병 및 부착규조에 의한 병해가 만연 하였다. 김 및 파래 발에 침착된 뻘의 양은 32.9-361.9mg/10cm였으며 침착된 뻘량과 병해율은 정의 상관관계가 나타났다. 뻘과 부착 조류는 사주와 사주 사이의 조간대에 위치한 양식어장에서 높게 나타났으며, 김과 파래의 엽면에 착생하는 부착 규조류는 Achnanthes, Licmophora, Melosira, Nitzschia, Synedra속으로 나타났다. 낙동강 하구 해역 해조류 양식장에서 발생되는 양식 해조의 병해 원인은 생활하수 및 공장폐수 등의 오염물질량의 증가와 주변 해수의 정체 그리고 단속적으로 방류되는 하천수에 의한 것으로 사료되며 특히 홍수 등의 대유량 빈도가 높을 때는 구멍갯병이 만연하는 것으로 사료된다. After the completion of the Naktong River estuary barrage, physiological damages and diseases of the cultivated marine algae in the estuary were investigated reviewing meteorological and environmental factors from September, 1989 to April, 1990. The meteorological characters in the precipitation, sunshine and air temperature were appaered at the region of Pusan during the farming period. COD and concentrations of nutrients of water seem to have increased in the just lower region of the barrage off Changrim, while pH and salinities decreased, probably due to the domestic sewage and industrial waste discharged from the Changrim stream and water stagnant after the barrage construction. The cultivated Gim, Porphyra and Parae, Enteromorpha have been infected with shot hole, green spot, red rot, white blight, crape and diatom felt. The amounts of mud adhered to the farming net were 32.9-361.9mg/10cm. There were correlations between diseases and mud. Most of the epjphytic microalgae on the farming net and thallus were genus Achnanthes, Licmopfora, Melosira, Nitzschia and Synedra, among them the dominant genus was Licmophora. Accordingly, effects of the diseases of Gim and Parae at the farming area in the estuary seem to have originated from the stagnation of water, the waste, and the discharge inflowing to farming area after the barrage construction.

기초저면의 조도가 기초의 침하 특성에 미치는 영향

유남재,김영길,박병수 강원대학교 산업기술연구소 1992 産業技術硏究 Vol.12 No.-

This research is to investigate the effect of base roughness of footing on characteristics of load-settlement curve. Parametric experiments of small scaled model test were performed with changing the properties of base roughness of model footing ; Gluing the vinyl, aluminum, sand paper, sand beneath the model footing surface. The width of model footing and relative density of soil foundation were also changed to investigate their effects on settlement characteristics of footing. The ultimate bearing capacity as well as the initial slope of load-settlement curve obtained from test results were compared with those from limit equilibrium methods proposed by Terzaghi, Hansen and Meyerhof. From test results, it was confirmed that the base roughness affected the failure mechanisms of showing different shapes of slip lines formed beneath the footing.

준설매립토지반의 압밀침하량 예측

유남재,박병수,정길수 江原大學校 産業技術硏究所 2001 産業技術硏究 Vol.21 No.A

For soils with high void ratios, the inverse method of utilizing results obtained from centrifuge model test was used to find the constitutive relation of effective stress - void ratio - permeability whereas conventional oedometer test arid constant rate of strain consolidation test were also used to fine its relation at ranges of relatively low void ratio. Results of column test about settlement of interface and pore pressure and distribution with time were compared with numerically estimated values to confirm such a constitutive relation as obtained from the inverse method. Consolidational settlement in dredged and reclaimed ground, where the consolidation was in progress, was predicted by using the numerical technique implemented with the finite strain consolidation theory.